Research Article | Volume: 6, Issue: 1, Jan-Feb, 2018

Nonconventional induction strategies for production of recombinant human tumor necrosis factor-alpha in Escherichia coli

Tapan Kumar Singha Pooja Gulati Sanjay Kumar   

Open Access   

Published:  Jan 17, 2018

DOI: 10.7324/JABB.2018.60105
Abstract

The present study investigates the production kinetics of recombinant human tumor necrosis factor-alpha (TNF-α) in Escherichia coli under three different nonconventional inducible expression systems, namely, salt, the thermal, and auto-inducible system in batch culture in a bioreactor. A codon-optimized synthetic gene of human TNF-α was inserted into plasmid pET-14b under T7 promoter to obtain its plasmid-based expression in E. coli. Very high specific yield (~47% of total cellular protein (TCP)) was obtained with auto-inducible expression system. The thermal and salt-induced expressions could yield up to ~30% and ~17% of TCP, respectively. Maximum specific productivities were reported to be 0.092 g/gDCW/h, 0.1 g/gDCW/h, and 0.079 g/gDCW/h for auto-inducible, thermal-inducible, and salt-inducible expression systems, respectively.


Keyword:     Tumor necrosis factor-alpha Expression kinetics Auto-induction Thermal induction Salt induction Escherichia coli.


Citation:

Singha TK, Gulati P, Kumar S. Nonconventional induction strategies for production of recombinant human tumor necrosis factor-alpha in Escherichia coli. J App Biol Biotech. 2018;6(1):23-27. DOI: 10.7324/JABB.2018.60105

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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