Research Article | Volume: 6, Issue: 1, Jan-Feb, 2018

Location and histopathology of seed-borne bacterial pathogen Pseudomonas syringae pv. pisi carried by pea seeds

Ashwani Kumar Verma Kailash Agrawal   

Open Access   

Published:  Jan 17, 2018

DOI: 10.7324/JABB.2018.60104
Abstract

The present study aims to determine the location and histopathology of seed-borne bacterial pathogen Pseudomonas syringae pv. pisi associated with pea seeds. The pea seeds categorized (asymptomatic, moderately discolored, and shrivelled discolored seeds) and seeds of each category were assayed for the presence of bacterial pathogen P. syringae pv. pisi. Seed samples carrying high incidence (89.5% and 91.34%) of the bacterial pathogen were used for the microtome sectioning individually. It was observed that the cells of P. syringae pv. pisi were found in large numbers in radicle and hilum region and space between seed coat and spermoderm in shrivelled discolored seed category while in moderately discolored seed category bacterial cells were less in numbers and found into the inner side of seed coat and endosperm. Bacterial colonization within seed tissue caused necrosis, formation of lytic cavities, and reduction in cell contents.


Keyword:     Categorization Incidence Pea seeds Pseudomonas syringae pv. pisi Symptoms.


Citation:

Verma AK, Agrawal K. Location and histopathology of seed-borne bacterial pathogen Pseudomonas syringae pv. pisi carried by pea seeds. J App Biol Biotech. 2018;6(1):20-22. DOI: 10.7324/JABB.2018.60104

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text
Reference

1. Anonymous. Food and agriculture organization of the United Nations; 2017. Available from: http://www.fao.org/faostat/en/#home. [Last accessed on 2017 May].

2. Verma AK, Agrawal K. Estimation of changes in enzymatic activity due to infection of seed-borne bacterial pathogens associated with pea (Pisum sativum L.) seeds. Int J Curr Adv Res 2016;5:1320-2.

3. Agarwal VK, Sinclair JB. Principles of Seed Pathology. 2nd ed. Boca Raton, FL, USA: CRC Press, Inc.; 2014. p. 539.

4. Agarwal VK. Seed Health. Lucknow: International Book Distributing Co.; 2006. p. 554.

5. Jain R, Agrawal K. Bio-efficacy of plant extracts against Pseudomonas syringae pv. syringae causing leaf spot of cluster bean. Ann Pl Protec Sci 2011;19:106-12.

6. Johanson DA. Plant Microtechniques. Vol. 11. New York: Tata McGraw Hill Book Company; 1940. p. 523.

7. Agarwal VK, Sinclair JB. Principles of Seed Pathology. Boca Raton, Florida, U.S.A: CRC Press, Inc.; 1987. p. 168.

8. Song WY, Kim HM, Hwang CY, Schaad NW. Detection of Acidovorax avenae sp. avenae in rice seeds using BIO-PCR. J Phytopathol 2004;152:667-76.
https://doi.org/10.1111/j.1439-0434.2004.00914.x

9. Godika S, Agrawal K, Singh T. Histopathological and biochemical changes in seeds of sunflower infected with Pseudomonas syringae. In: Proceeding. of Indian Phytopathological Society-Golden Jubilee: International conference. Integrated plant Disease Management of Sustainable Agriculture. Vol. II. New Delhi: IARI; 2000. p. 1131-2.

10. Verma AK, Arora P, Agrawal K. Incidence of bacterial blight pathogen Pseudomonas syringae pv. pisi in pea seeds grown in Rajasthan, India. Legume Res 2016;39:1034-7.
https://doi.org/10.18805/lr.v0iOF.8606

11. Sharma DK, Agrawal K. Incidence and histopathology of Ralstonia solanacearum in tomato seeds. J Mycol Plant Pathol 2010;40:115-9.

12. Adorada DL, Stodart BJ, Pangga IB, Ash GJ. Implications of bacterial contaminated seed lots and endophytic colonization by Pseudomonas fuscovaginae on rice establishment. Plant Pathol 2015;64:43-50.
https://doi.org/10.1111/ppa.12243

13. Sharma M, Kumar D, Agrawal K, Singh T, Singh, D. Colonization of pigeonpea seed by Xanthomonas campestris pv. cajani. J Mycol Plant Pathol 2001;31:216-9.

14. Rudolph K, Burr TJ, Mansfield J, Stead DE, Vivian A, Kietzell JV. Pseudomonas syringae pathovars and related pathogens. Kluwer academic publisher, USA: Springer Science & Business Media; 2012. p. 665.

15. Dutta B, Avci U, Hahn MG, Walcott RR. Location of Acidovorax citrulli in infested watermelon seeds is influenced by the pathway of bacterial invasion. Phytopathology 2012;102:461-8.
https://doi.org/10.1094/PHYTO-10-11-0286-R

16. Skoric V. Bacterial blight of pea: Overwintering, dissemination and pathological histology. Phytopathology 1927;17:611-27.

17. Rashid AQ. Detection of seed-borne Pseudomonas syringae pv. syringae in wheat. Plant Varieties Seeds 1995;8:47-54.

Article Metrics

50 Absract views 94 PDF Downloads 144 Total views

Related Search

By author names

Citiaion Alert By Google Scholar