Research Article | Volume: 5, Issue: 6, Nov-Dec, 2017

Optimization of fermenting medium by statistical method for production of alkaline protease by Bacillus licheniformis MZK05M9

Md. Arafat Al Mamun Md. Mahmuduzzaman Mian Mohammad Saifuddin Shakila Nargis Khan Md. Mozammel Hoq   

Open Access   

Published:  Nov 09, 2019

DOI: 10.7324/JABB.2017.50604
Abstract

To optimize the fermentation medium for production of alkaline protease by Bacillus licheniformis MZK05M9 (BlM9) molasses as carbon source, soybean meal as nitrogen source and the salts NaCl, MgSO4.7H2O and K2HPO4 were selected by Plackett-Burman approach. The Response Surface Methodology (RSM) based on Central Composite Design (CCD) revealed that the optimum values for the tested variables were found as (% w/v) molasses 0.92, soybean meal 0.79, NaCl 0.125, MgSO4 0.125 and K2HPO4 0.59 with the protease activity 761 U/ml predicted by statistical software Minitab Version 17. The experimental value was found as 765 U/ml. The granular size of soybean meal 4.7 mm supported the enzyme production 5 % higher than that of the mixed sizes between 6 to 4 mm. Fermentation in 7 l bioreactor exhibited the enzyme activity 1020 U/ml after 28 h. The statistically optimized medium based on cost-effective agro-industrial C and N sources yielded a high productivity 36428 U/l h of protease by the mutant strain of B. licheniformis.


Keyword:     Bacillus licheniformis MZK05M9 Alkaline protease Molasses Soybean meal Granular size.


Citation:

Al Mamun MA, Mian MM, Saifuddin M, Khan SN, Hoq MM. Optimization of fermenting medium by statistical method for production of alkaline protease by Bacillus licheniformis MZK05M9. J App Biol Biotech. 2017;5(6):24-28. DOI: 10.7324/JABB.2017.50604

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Reference

Shivasharanappa K, Hanchinalmath JV, Sundeep YS, Borah D, Talluri VS. Optimization and production of alkaline proteases from agro byproducts using a novel Trichoderma viridiae strain VPG 12 isolated from agro soil. Int Lett Nat Sci 2014;14:77-84.

2. Reddy LV, Wee YJ, Yun JS, Ryu HW. Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett-Burman and response surface methodological approaches. Bioresour Technol 2008;99:2242-9.

3. Raja CA, Prabhahar C. Screening and optimization of alkaline protease productivity from Bacillus sp. Agt from tannery effluent. Int J Pharm Biol Arch 2012;3:244-8.

4. Hoq MM, Mamun AA, Shishir MA, Khan MM, Akand MN, Khan SN. Bioprocess Development for Eco-Friendly Microbial Products and Impacts on Bio-Industry Establishment in Bangladesh, Proceedings of International Conference on Biotechnology, 25-26 May; 2013.

5. Davatia N, Najafib MB. Overproduction strategies for microbial secondary metabolites: A review. Int J Life Sci Pharm Res 2013;3:23-37.

6. Mamun MA, Khan MM, Akand MN, Khan SN, Hoq MM. Characterization of an alkaline protease with high quality bating potential in leather processing from Bacillus licheniformis MZK05M9 mutant. Int J Biol Res 2015;3:36-41.

7. Puri S, Beg QH, Gupta R. Optimization of alkaline protease production from Bacillus sp. by response surface methodology. Curr Microbiol 2002;44:286-90.

8. Li J, Ma C, Ma Y, Li Y, Zhou W, Xu P. Medium optimization by combination of response surface methodology and desirability function: an application in glutamine production. Appl Microbiol Biotechnol 2007;74:563-71.

9. Xiao ZJ, Liu PH, Qin JY, Xu P. Statistical optimization of medium components for enhanced acetoin production from molasses and soybean meal hydrolysate. Appl Microbiol Biotechnol 2007;74:61-8.

10. Bhunia B, Basak B, Dey A. A review on production of serine alkaline protease by Bacillus spp. J Biochem Technol 2012;3:448-57.

11. Salaheen S, Mamun MA, Khan SN, Hoq MM. Improvement of Bacillus licheniformis MZK05 by mutation for increased production of keratinase. Dhaka Univ J Biol Sci 2015;24:17-23.

12. Kreger A, Lockwood D. Detection of extracellular toxin produced by Vibrio vulnificus. Infect Immun 1981;33:588-90.

13. Dey G, Mitra A, Banerjee R, Maiti BR. Enhanced production of amylase by optimization of nutritional constituents using response surface methodology. Biochem Eng J 2001;7:227-31.

14. Chauhan B, Gupta R. Application of statistical experimental design for optimization of alkaline protease production from Bacillus sp. RGR-14. Process Biochem 2004;39:2115-22.

15. Nadeem M, Qazi JI, Baig S, Syed QA. Effect of medium composition on commercially important alkaline protease production by Bacillus licheniformis N-2. Food Technol Biotechnol 2008;46:388-94.

16. Saxena R, Singh R. Statistical optimization of conditions for protease production from Bacillus sp. Acta Biol Szeged 2010;54:135-41.

17. Irfan M, Nadeem M, Syed Q, Nawaz W, Baig S, Qureshi AM. Relationship of process parameters for the production of alkaline protease by Bacillus sp. Int J Agric Vet Med Sci 2010;4:114-20.

18. Zambare VP, Nilegaonkar SS, Kanekar PP. Protease production and enzymatic soaking of salt-preserved buffalo hides for leather processing. IIOAB Lett 2013;3:1-7.

19. Saurabh S, Jasmine I, Pritesh G, Kumar SR. Enhanced productivity of serine alkaline protease by Bacillus sp. Using soybean as substrate. Malaysia J Microbiol 2007;3:1-6.

20. Oberoi R, Beg QK, Puri S, Saxena RK, Gupta R. Characterization and wash performance analysis of an SDS-stable alkaline protease from a Bacillus sp. World J Microbiol Biotechnol 2001;17:493-7.

21. Lakshmi BK, Sri PV, Devi KA, Hemalatha KP. Screening, optimization of production and partial characterization of alkaline protease from haloalkaliphilic Bacillus sp. Int J Res Eng Technol 2014;3:435-43.

22. Wahyuntari B, Hendrawati H. Properties of an extracellular protease of Bacillus megaterium DSM 319 as deilating aid of hides. Microbiol Indones 2012;6:77-82.

23. Zambare VP, Nilegaonkar SS, Kanekar PP. Production of an alkaline protease by Bacillus cereus MCM B-326 and its application as a dehairing agent. World J Microbiol Biotechnol 2007;23:1569-74.

24. Naidu KS, Devi KL. Optimization of thermostable alkaline protease production from species of Bacillus using rice bran. Afr J Biotechnol 2005;4:724-6.

25. Olajuyigbe FM. Optimized production and properties of thermostable alkaline protease from Bacillus subtilis SHS-04 grown on groundnut (Arachis hypogaea) meal. Adv Enzyme Res 2013;1:112-20.

26. Younis MA, Hezayen FF, Nour-Eldein MA, Shabeb MS. Production of protease in low-cost medium by Bacillus subtilis KO strain. Glob J Biotechnol Biochem 2009;4:132-7.

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