Research Article | Volume: 5, Issue: 6, Nov-Dec, 2017

Optimization of fermenting medium by statistical method for production of alkaline protease by Bacillus licheniformis MZK05M9

Md. Arafat Al Mamun Md. Mahmuduzzaman Mian Mohammad Saifuddin Shakila Nargis Khan Md. Mozammel Hoq   

Open Access   

Published:  Nov 09, 2019

DOI: 10.7324/JABB.2017.50604

To optimize the fermentation medium for production of alkaline protease by Bacillus licheniformis MZK05M9 (BlM9) molasses as carbon source, soybean meal as nitrogen source and the salts NaCl, MgSO4.7H2O and K2HPO4 were selected by Plackett-Burman approach. The Response Surface Methodology (RSM) based on Central Composite Design (CCD) revealed that the optimum values for the tested variables were found as (% w/v) molasses 0.92, soybean meal 0.79, NaCl 0.125, MgSO4 0.125 and K2HPO4 0.59 with the protease activity 761 U/ml predicted by statistical software Minitab Version 17. The experimental value was found as 765 U/ml. The granular size of soybean meal 4.7 mm supported the enzyme production 5 % higher than that of the mixed sizes between 6 to 4 mm. Fermentation in 7 l bioreactor exhibited the enzyme activity 1020 U/ml after 28 h. The statistically optimized medium based on cost-effective agro-industrial C and N sources yielded a high productivity 36428 U/l h of protease by the mutant strain of B. licheniformis.

Keyword:     Bacillus licheniformis MZK05M9 Alkaline protease Molasses Soybean meal Granular size.


Al Mamun MA, Mian MM, Saifuddin M, Khan SN, Hoq MM. Optimization of fermenting medium by statistical method for production of alkaline protease by Bacillus licheniformis MZK05M9. J App Biol Biotech. 2017;5(6):24-28. DOI: 10.7324/JABB.2017.50604

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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