Research Article | Volume: 5, Issue: 4, July-August, 2017

Fermentative Production of Microbial Enzymes and their Applications: Present status and future prospects

Viswanath Vittaladevaram   

Open Access   

Published:  Aug 14, 2017

DOI: 10.7324/JABB.2017.50414

Microbial enzymes are widely used in different industries mainly because of vast availability of sources. Microbial enzymes could be genetically modified and are considered as economical in comparison to plant and animal enzymes. Production of microbial enzymes by application of fermentation procedures involves microbial propagation to get desired product. The process of fermentation is classified based on specific parameters. Microbial enzymes exhibit wide variety of applications in different industries like food, wine, dairy, baking, milling, beverages, and cereals. There are different techniques employed to produce microbial enzymes using downstream processing methods that are aimed at enzyme purification and recovery. The improvement in concentration, purity and percentage of recovery of enzymes can be achieved based on standard principles which are microbial sources, improvement of strain and application of membrane augmented downstream processing method to improve specific activity of enzyme. The article reviews on principles include microbial sources, methods of strain improvement and modern techniques associated with improvement of enzyme activity and recovery process. The application of microbial enzymes in various industries and their importance in biotechnology is highlighted.

Keyword:     Enzyme activity Enzyme recovery Membrane filtration technique Microbial sources Strain improvement.


Vittaladevaram V. Fermentative Production of Microbial Enzymes and their Applications: Present status and future prospects. J App Biol Biotech. 2017; 5 (04): 090-094.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Amro A, Soheir SR. Degradation of castor oil and lipase production by Pseudomonas aeruginosa. Journal of Agricultural & Environmental Sciences. 2009; 5(4):556–563.

2. Ahlawat S,Dhiman SS, Battan B,Mandhan RP,Sharma J. Pectinase production by Bacillus subtilis and its potential application in biopreparation of cotton and micropoly fabric. Process Biochemistry. 2009; 44(5):521–526.

3. Sabir S, Bhatti HN, Zia MA, Sheikh MA. Enhanced production of glucose oxidase using Penicillium notatum and rice polish. Food Technology and Biotechnology.2007; 45(4):443–446.

4. Sharma R, Chisti Y, Banerjee UC. Production, purification, characterization, and applications of lipases. Biotechnology advances. 2001; 19(8):627–662.

5. Genckal H, Tari, C. Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats. Enzym. Microb. Technol. 2006; 39:703–710.

6. Kalishwaralal K, Gopalram S, Vaidyanathan R, Deepak V, Pandian SRK, Gurunathan S. Optimization of α-amylase production for the green synthesis of gold nanoparticles. Colloid. Surf. B. Interf. 2010; 77:174-180.

7. Sarrouh B,Santos TM,Miyoshi A,Dia R, Azevedo V. Up-To-Date insight on industrial enzymes applications and global market. Journal of Bioprocessing & Biotechniques. 2012; S:4-002.

8. Sivaramakrishnan S, Gangadharan D, Nampoothiri KM, Soccol CR, Pandey A. α-amylases from microbial sources – An overview on recent developments. Food Technol. Biotechnol. 2006; 44:173–184.

9. Gurumurthy DM, Neelagund SE. Molecular characterization of industrially viable extreme thermostable novel alpha-amylase of geobacillus sp Iso5 Isolated from geothermal spring. J. Pure Appl. Microbiol. 2012; 6:1759–1773.

10. Nigam P, Pandey A. Eds. Biotechnology for Agro-Industrial Residues Utilisation; Publisher Springer Science Business Media B.V. 2009; pp. 1–466.

11. Chirumamilla RR, Muralidhar R, Marchant R, Nigam P. Improving the quality of industrially important enzymes by directed evolution. Mol. Cell. Biochem. 2001; 224:159–168.

12. Chudasama CJ, Jani SA, Jajda HM, Pate HN. Optimization and production of alkaline protease from Bacillus thuringiensis CC7. J. Cell Tissue Res. 2010; 10:2257–2262.

13. Vijayalakshmi S, Venkat Kumar S, Thankamani V. Optimization and cultural characterization of Bacillus RV.B2.90 producing alkalophilic thermophilic protease. Res. J. Biotechnol. 2011; 6:26–32.

14. Li Y, Niu D, Zhang L, Wang Z, Shi G. Purification, characterization and cloning of a thermotolerant isoamylase produced from Bacillus sp. CICIM 304. J. Ind. Microbiol. Biotechnol. 2013; 40:437–446.

15. Li Q, Yi L, Marek P, Inverson BL. Commercial Proteases: Present and future. FEBS Lett. 2013; 587:1155-1163.

16. Verma N, Thakur S, Bhatt AK. Microbial Lipases: Industrial Applications and Properties. International Research Journal of Biological Sciences. 2012; 1:88-92.

17. Das S, Singh S, Sharma V, Soni ML. Biotechnological applications of industrially important amylase enzyme. International Journal of Pharma and Bio Sciences. 2011; 2(1):486–496.

18. Binod P, Palkhiwala P, Gaikaiwari R. Industrial enzymes: present status and future perspectives for India: present scenario and perspectives. J Sci Ind Res. 2013; 72:271–286.

19. Adrio JL, Demain AL. Microbial enzymes: tools for biotechnological processes. Biomolecules. 2014; 4(1):117–139.

20. Choi JM, Han SS, Kim HS. Industrial applications of enzyme biocatalysis: current status and future aspect. Biotechnol Adv. 2015; 33:1443–1454.

21. Maria Alice ZC,Bernardo DR.White Biotechnology for Sustainable Chemistry. Royal Society of Chemistry: E-Publishing Inc; 2016, pp. 198.

22. Binod P, Piyush P, Raghavendra G, Madhavan KN, Arvind D, Kakali D, Ashok P. Industrial enzymes-Present status and future perspectives for India. Journal of Scientific and Industrial Research. 2013;72:271-286.

23. Nigam P. Microbial enzymes with special characteristics for biotechnological applications. Biomolecules.2015; 3(3): 597–611.

24. Seo YB, Park J, Huh IY, Hong SK, Chang YK. Agarose hydrolysis by two-stage enzymatic process and bioethanol production from the hydrolysate. Process Biochem. 2016; 51:759–764.

25. Rodríguez V, Asenjo JA, Andrews BA.Design and implementation of a high yield production system for recombinant expression of peptides. Microb Cell Fact. 2014; 13:1–10.

26. Rajendra S,Manoj K,Anshumali M,Praveen KM. Microbial enzymes: industrial progress in 21st century. Biotech. 2016; 6(2):174.

27. Adrio JL, Demain AL. Microbial Enzymes: Tools for Biotechnological Processes. Biomolecules. 2014; 4: 117-139.

28. Arnold LD, Preeti V. Production of recombinant proteins by microbes and higher organisms. Biotechnology Advances.2009; 27: 297-306.

29. Bueno MM, Thys RCS, Rodrigues RC. Microbial enzymes as substitutes of chemical additives in baking wheat flour—Part II: combined effects of nine enzymes on dough rheology. Food Bioproces. Technol. 2016; 9:1598–1611.

30. Liu Y, Lin S, Zhang X, Liu X, Wang J, Lu F. A novel approach for improving the yield of Bacillus subtilis transglutaminase in heterologous strains. J. Ind. Microbiol. Biotechnol. 2014; 41:1227–1235.

31. Vengadaramana A. Industrial Important Microbial alpha-Amylase on Starch-Converting Process., Sch. Acad. J. Pharm.2013; 2(3):209-221.

32. Singh R, Kumar M, Mittal A, Mehta PK. Microbial enzymes: Industrial progress in 21st century. Biotech. 2016; 6:174.

33. Tiwari SP, Srivastava R, Singh CS, Shukla K, Singh RK, Singh P, Singh R, Singh NL,Sharma R. Amylases: An overview with special reference to alpha amylase. Journal of Global Biosciences. 2015; 4(1):1886-1901.

34. Sundarram A, Murthy TPK. α-Amylase Production and Applications: A Review Journal of Applied & Environmental Microbiology. 2014; 2(4):166-175.

35. Garg G, Singh A, Kaur A, Singh R, Kaur J, Mahajan R. Microbial pectinases: an ecofriendly tool of nature for Industries. Biotech. 2016; 6(1):47-59.

36. Aguilar-Toalá JE, Santiago-López L, Peres CM, Peres C, Garcia HS, Vallejo-Cordoba B, González-Córdova AF, Hernández-Mendoza A. Assessment of multifunctional activity of bioactive peptides derived from fermented milk by specific Lactobacillus plantarum strains. Journal of Dairy Science. 2016; 100:65–75.

37. Agyei D, Ongkudon CM, Wei CY, Chan AS, Danquah MK. Bioprocess challenges to the isolation and purification of bioactive peptides. Food and Bioproducts Processing. 2016; 98:244–256.

38. Zhang Q, Han Y, Xiao H. Microbial α-amylase: A biomolecular overview. Process Biochemistry. 2017; 53:88–101.

39. Hussain I, Siddique F, Mahmood MS, Ahmed SI. A review of the microbiological aspect of alpha-amylase production. Int J Agric Biol. 2013; 15:1029–34.

40. Suganthi R, Benazir JF, Santhi R, Ramesh Kumar V, Anjana Hari, Nitya Meenakshi, Nidhiya KA, Kavitha G, Lakshmi R. Amylase Production by Aspergillus Niger Under Solid State Fermentation Using Agroindustrial Waste. International Journal of Engineering Science and Technology. 2011; 3(2):1756-1763.

41. Patel A, Singhania R, Pandey A. Novel enzymatic processes applied to the food industry. Curr. Opin. Food Sci. 2016; 7:64–72.

42. Andualema B, Gessesse A. Microbial lipases and their industrial applications: review. Biotechnology.2012; 11:100-118.

43. Dalmaso GZL, Ferreira D, Vermelho AB. Marine extremophiles: a source of hydrolases for biotechnological applications. Mar. Drugs. 2015; 13:1925–1965.

44. Dionisi HM, Lozada M, Olivera NL. Bioprospection of marine microorganisms: biotechnological applications and methods. Rev. Argent. Microbiol. 2012; 44:49–60.

45. Ferrer M, Martínez-Martínez M, Bargiela R, Streit WR, Golyshina OV, Golyshin PN. Estimating the success of enzyme bioprospecting through metagenomics: current status and future trends. Microb. Biotechnol. 2016; 9:22–34.

46. Zhang C, Kim SK. Application of marine microbial enzymes in the food and pharmaceutical industries. Adv. Food Nutr. Res. 2012; 65:423–435.

47. Pallavi Pogaku P, Bhavani T, Manjusha, Saritha K. Lipases in Environmental Management. Int. J. Curr. Res. Aca. Rev. 2017; 5(4):106-109.

48. Mehta A, Saun NK, Gupta R. Purification and Characterization of Lipase from thermophilic Geobacillus sp, Current Biotechnology. 2016; 5 (1):81-89.

49. Muthumari GM, Thilagavathi S, Hariram N. Industrial Enzymes: Lipase Producing Microbes from Waste Volatile Substances. Int J Pharm Sci Res. 2016; 7(5):2201-08.

50. Sundarram A, Krishna Murthy TP. α-Amylase production and applications: A Review. Journal of Applied & Environmental Microbiology. 2014; 2(4):166–175.

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