Published:  Jun 19, 2017DOI: 10.7324/JABB.2017.50306
One of the reasons for the plaque formation in Alzheimer’s Disease (AD) is the metal induced aggregation of Aβ(1-42). Its C-terminal hydrophobic residues are generally found inside the membrane; but the exposed regions (1-28) are predominantly ligand interacting and believed to be responsible for onset of aggregation events. Recent evidences have indicated that the smaller fragments of Aβ like (17-28), (1-16) and (1-10) are also produced in presence of secretases and elastase. In this background, the current work focuses upon assessing the binding patterns of the residues contained in the smaller fragments (such as 1-16) with metals like zinc, copper, aluminium, and small molecules like betaine and curcumin, via Circular Dichroism (CD) and computational docking methods. The CD data and in silico exercises offer valuable information about the determinants that take part in ligand binding and thus contribute to the wealth of knowledge towards appreciating the triggering events related to aggregation patterns of AD. These results not only provide insights into the mechanism that underlie the formation of toxic fragments, but also suggest design of molecules that could function as plausible breakers of the progression of Alzheimer’s disease (AD).
Narayan P, Jagadeesh Kumar D, Govinda Raju M, Nagendra H G, Easwaran KRK. CD and Computational studies on Aβ (1-16) suggests determinants of ligand binding and plausible prevention of metal induced toxicity via Betaine like molecules. J App Biol Biotech. 2017; 5 (03): 030-038. DOI: 10.7324/JABB.2017.50306
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