Research Article | Volume: 5, Issue: 3, May-June, 2017
Dagaa (Rastrinoebola argentea) protein hydrolysate as a nitrogen source in microbial culture media
Dagaa is a small pelagic fish found in Lake Victoria. It is used as a nutrient source in animal feeds and human consumption. Utilization of fish protein hydrolysate as a nutrient in culture media has been explored in several fish species and dagaa protein hydrolysate (DPH) can also be a candidate due to its rich protein and lipid content. Potential of DPH as a nitrogenous source in microbial growth media was assayed using the following bacteria; Escherichia coli, Pseudomonas aeroginosa, Bacillus subtilis and Rhodobacter capsulatus and two fungi, Metarhizium anisopliae and Beauveria bassiana. Cell growth was evaluated using turbidity and biomass in liquid cultures. DPH formulated media produced similar cell growth when compared to positive controls made up of Luria Bertani (LB) for bacteria and Sabouraud’s dextrose broth (SDB) for fungi. This indicates that formulated DPH media can be considered as an alternative media for microbial culture.
Pere K, Mbatia BN, Muge E, Wekesa VW. Dagaa (Rastrinoebola argentea) protein hydrolysate as a nitrogen source in microbial culture media. J App Biol Biotech. 2017; 5 (03): 008-012.
1. Nair AJ. Introduction to Biotechnology and Genetic Engineering. In Introduction to Biotechnology and Genetic Engineering. Infinity Science Press Llc. 2008 P. 689-699.
2. Asimov SI, Horn SJ, Eosin VGH.Use of hydrolysates from Atlantic cod (Gadusmorhua L.) viscera as a complex nitrogen source for lactic acid bacteria. FEMS Microbiol. Letters.2005; 248(1), 65–68.
3. Martone CB, Pérez BorlaO, Sánchez J J. Fishery by-product as a nutrient source for bacteria and archaea growth media. Biores. Technol. J.2005; 96 (3), 383–7. http://doi.org/10.1016/j.biortech.2004.04.008
4. Ovissipour M, Safari R, Motamedzadegan A, Rasco B, Pourgholam R, Mohagheghi E, Molla AE. Use of hydrolysates from yellow fin tuna Thunnus albacares fisheries by-product as a nitrogen source for bacteria growth media. Internat. Aquatic Res. 2009; 1(1), 73-77.
5. Olivieri, R., Sabbatini, F., Kontakou, M., Tagliaferri, L., Giglioli, A., &Rappuoli, R. (2016). U.S. Patent No. 9,284,526. Washington, DC: U.S. Patent and Trademark Office.
6. Beaulieu L, Desbiens M, Thibodeau J,Thibault S. Pelagic fish hydrolysates as peptones for bacterial culture media. Can. J.Microbiol. 2009; 55(11), 1240-1249.
7. Safari R, Nasrollahzadeh Saravi H, Pourgholam R, Motalebi AA, Ghoroghi A. Use of Hydrolysates from Silver Carp (Hypophthalmichthys molitrix) Head as Peptone for Vibrio anguillarum and Optimization Using Response Surface Method (RSM). J.Aquatic Food Product Technol. 2011;20 (2), 247–257. http://doi.org/10.1080/10498850.2011.562064
8. Fallah M, BahramS, Javadian SR.Fish peptone development using enzymatic hydrolysis of silver carp by-products as a nitrogen source in Staphylococcus aureus media. Food Sci. Nutrit. 2015;3 (2), 1-5. http://doi.org/10.1002/fsn3.198
9. Shu G, Zhang Q, Chen H, Wan H, Li H. Effect of Five Proteases Including Alcalase, Flavourzyme, Papain, Proteinase K And Trypsin On Antioxidative Activities Of Casein Hydrolysate From Goat Milk. Acta Universitatis Cibiniensis. Series E: Food Technology, 2015; 19 (2), 65-74.
10. Klompong V, Benjakul S, Kantachote D,Shahidi F. Characteristics and use of yellow stripe trevally hydrolysate as culture media. J. Food Sci.2009; 74(6), 219-225.
11. Nyeko D. Challenges in sharing of Lake Victoria Fisheries Resources: Policies, Institutions and Processes. LVFO Regional Stakeholder’s Conference Kampala. Sponsored by LVFO, European Union and CTZ, 2008.
12. SaleheM, Mlaponi E, Onyango PO, Mrosso Hilary DJ. Contribution of Lake Victoria Dagaa fishery in East and Central African fish trade. 16th IIFET Conference, July 16-20, 2012, Dar es Salaam, Tanzania
13. Owaga EE, Onyango CA, Njoroge KA. Influence of selected washing treatments and drying temperatures on proximate composition of dagaa (Rastrineobola argentea), a small pelagic fish species. Afr. J. Food Agric. Nutrit. Dev. 2010; 10, (7).
Year
Month