The biocontrol measures against pathogens are widely recognized as a step towards organic agriculture. In the present study, nine species of endophytic fungi and four plant pathogenic fungi were tested against antagonistic activity of a root endophyte, Trichoderma viride. Out of nine endophytic fungi, eight were inhibited. The inhibitory effect was maximum against Alternaria sp. and Pythium sp. The minimum zone was noticed against Nigrospora sp. after 14 days of incubation. It showed no inhibitory effect against an isolate of Aspergillus sp. and overgrew it after 9 days of incubation. T. viride showed antagonistic activity against all the four plant pathogenic fungi. The antagonistic zone was highest against Colletotrichum capsici and lowest was observed in Alternaria solani after 14 days of incubation. The study suggests the potential of an endophytic strain of fungus and it may be used in biocontrol programmes of plant diseases in the region.
Talapatra K, Das AR, Saha AK, Das P. In vitro antagonistic activity of a root endophytic fungus towards plant pathogenic fungi. J App Biol Biotech. 2017; 5 (02): 068-071. DOI: 10.7324/JABB.2017.50210
1. Vinale FK, Sivasithamparam EL, Ghisalberti R, Marra SL, Lento M. Trichoderma plant pathogens interactions. Soil Biology and Biochemistry. 2008; 40:1-10.
2. Cook RJ, Granados RR. Biological control: making it work. In: MacDonald MJF, editor. Agricultural biotechnology at the crossroads. Ithaca (NY). National Agricultural Biotechnology Council. 1991; 213-227.
3. Barakat RM, Al-Masri MI. Bilogical control of gray mold disease (Botrytis cinerea) on tomato and bean plants by using local isolates of Trichoderma harzianum. Dirasat Agricultural Sciences. 2005; 32(2):145-156.
4. Harman GE. Overview of mechanisms and uses of Trichoderma spp. Phytopathology. 2006; 96(2):190-194.
5. Harman GE, Howell CR, Viterbo A, Chet I, Lorito M. Trichoderma species opportunistic, a virulent plant symbionts. Nature Reviews Microbiology. 2004; 2:43-56.
6. Shoresh M, Harman GE, Mastouri F. Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review of Phytopathology. 2010; 48:21-43.
7. Cummings NJ, Ambrose A, Braithwaite M, Bissett J, Roslan HA, Abdullah J, Stewart A, Agbayani FV, Steyaert J, Hill RA. Diversity of root-endophytic Trichoderma from Malaysian Borneo. Mycological Progress. 2016; 15:50 DOI 10.1007/s11557-016-1192-x.
8. Campanile G, Ruscelli A, Luisi N. Antagonistic activity of endophytic fungi towards Diplodia corticola assessed by in vitro and in planta tests. European Journal of Plant Patholology. 2007; 117:237-246.
9. Domsch KH, Gams WH, Anderson TH. 1980. Compendium of soil fungi, Vol-1, Academic Press (London).
10. Ellis MB. 1971. Dematiaceous hypomycetes. CAB International. Wallingford. UK.
11. Watanabe T. Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species (2nd ed.). 2002. CRC Press LLC, NW Corporate Blvd, Boca Raton, Florida.
12. Dennis C, Wesbter J. Antagonistic properties of species-group of Trichoderma. III. Hyphal Interactions. Transactions of the British Mycological Society. 1971; 57:363-369.
13. Ziedan El-Sayed HE, Farrag Eman SH, Sahab AF. Effect of Trichoderma harzianum against Thielaviopsis paradoxa and their pathological potential on date palm seedlings. Journal of Agricultural Technology. 2015; 11(4):913-923.
14. Naik BS, Shashikala J, Krishnamurthy YL. Study on the diversity of endophytic communities from rice (Oryza sativa L.) and their antagonistic activities in vitro. Microbiological Research. 2009; 164:290-296.
15. Terhonen E, Kerio S, Sun H, Asiegbu FO. Endophytic fungi of Norway spruce roots in boreal pristine mire, drained peatland and mineral soil and their inhibitory effect on Heterobasidion parviporum in vitro. Fungal ecology. 2014; 9:17-26.
16. Martínez-Álvarez P, Alves-Santos FM, Diez JJ. In vitro and in vivo interactions between Trichoderma viride and Fusarium circinatum. Silva Fennica. 2012; 46(3):303-316.
17. Mukherjee PK, Raghu K. Effect of temperature on antagonistic and biocontrol potential of Trichoderma sp. on Sclerotium rolfsii. Micropathologia. 1997; 139:151-155.
18. El-Katatny MH, El-Katatny MS, Fadl-Allah EM, Emam AS. Antagonistic effect of two isolates of Trichoderma harzianum against postharvest pathogens of tomato (Lycopersicon esculentum). Archives of Phytopathology and Plant Protection. 2011; 44(7):637-654.
19. Fajola AO, Alasoadura SO. Antagonistic effects of Trichoderma harzianum on Pythium aphanidermatum causing the Damping off disease of tobacco in Nigeria. Mycopathologia. 1975; 57(1):47-52.
20. Rajathilagam R, Kannabiran B. Antagonistic effects of Trichoderma viride against anthracnose fungus Colletotrichum capsici. Indian Phytopathology. 2001; 54(1):135-136.
21. Ganie SA, Ghani MY, Nissar Q, Shabir-u-Rehman. Bioefficacy of plant extracts and biocontrol agents against Alternaria solani. African Journal of Microbiology Research. 2013; 7(34):4397-4402.
22. Naglot A, Goswami S, Rahman I, Shrimali DD, Yadav KK, Gupta VK, Rabha AJ, Gogoi HK, Veer V. Antagonistic potential of native Trichoderma viride strain against potent tea fungal pathogens in North East India. The Plant Pathology Journal. 2015; 31(3):278-289.
23. Sundaramoorthy S, Balabaskar P. Biocontrol efficacy of Trichoderma spp. against wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici. Journal of Applied Biology and Biotechnology. 2013; 1 (03): 36-40.
24. Benitez T, Rincn AM, Limn MC, Codn AC. Biocontrol mechanisms of Trichoderma strains. International Microbiology. 2004; 7:249-260.
25. Elad Y. Biological control of foliar pathogens by means of Trichodema harzianum and potential modes of action. Crop Protection. 2000; 19:709-714.
26. Zeilinger S, Galhaup C, Payer K, Woo SL, Mach R.L, Fekete C, Lorito, M, Kubicek CP. Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genetics and Biology. 1999; 26:131-140.
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