Research Article | Volume: 5, Issue: 2, March-April, 2017

Comparative proteome analysis and thermal stress induced changes in the embryo of poly- and bi-voltine strains of Bombyx mori

Punyavathi Muzafer A. Bhat Manjunatha H. Boregowda   

Open Access   

Published:  Mar 20, 2017

DOI: 10.7324/JABB.2017.50209

Proteins are the ultimate operating molecules producing the physiological effect in all the stages of an organism. As a consequence, proteome of the embryo play a pivotal role in determining the biological structures besides cellular organization and morphogenetic movements that occur during embryo development. But, changes in the environment temperature for few hours whether that alter the embryo development, protein expression and hatching of infants are unknown in Bombyx mori L. Hence, eggs of poly- and bi-voltine breeds were exposed to heat shock temperature of 35, 40 and 45°C for 2 h to measure tolerance level and differential expression of proteins in different age of the embryos. To facilitate comparative analysis among different breeds a comprehensive proteome map was constructed considering protein profile of different day’s embryos. Interestingly, ~10 to 21 protein bands were recorded during embryo development - from ovipostion till hatching - along with few new protein bands on day-6, which were differ in their intensity in different silkworm strains. Interestingly, no change in the protein pattern was observed in different days embryos exposed to thermal stress at 35 and 40°C. But degradation of 18 and 19 kDa proteins were recorded in all the days of embryos exposed to 45°C while four major protein bands remain unaltered. Further, the percent of hatching was slightly declined in 35 and 40°C and zero hatching was recorded in all the stages of eggs HS at 45°C. This clearly indicates that differential expression/degradation of proteins as a response to thermal stress not only influences the growth and development but also determine hatching of embryo. Accordingly, we suggest that embryonic protein map constructed in the present study shall be a valid reference for comparative analysis and hatching of embryos as one of the key traits to measure the rate of thermotolerance in the existing or new strains/breeds of B. mori.

Keyword:     Bombyx mori Densitometry Embryo Global warming Heat shock Proteome.


Punyavathi, Bhat MA, Manjunatha HB. Comparative proteome. Comparative proteome analysis and thermal stress induced changes in the embryo of poly- and bi-voltine strains of Bombyx mori. J App Biol Biotech. 2017; 5 (02): 059-067. DOI: 10.7324/JABB.2017.50209

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. King AM, MacRae TH. Insect heat shock proteins during stress and diapause. Annu. Rev. Entomol. 2015; 60:59–75.

2. Nazia C, Mal Reddy N, Basavaraja HK, Dandin B. Alteration in qualitative and quantitative characters in bivoltine silkworm, Bombyx mori L. due to thermal stress. Indian J. Seric. 2005 1: 100-106.

3. Manjunatha HB, Rajesh RK, Aparna HS. Silkworm thermal biology: A review of heat shock response, heat shock proteins and heat acclimation in the domesticated silkworm, Bombyx mori. J. Insect Science. 2010; 10:204 available online:

4. Tazima. In: The Silkworm: an important laboratory tool. (Ed. By Tazima Y) Kodansha Ltd., Tokyo. 1978; 213-245.

5. Myohara M. Fate mapping of the larval silk glands of Bombyx mori by UV laser irradiation of the egg at fertilization. Dev. Genes and Evol. 2003; 213:178-181.

6. Manjunatha HB, Hosagoudar SR, Inamdar SR. Biomolecular changes and somatic mutations induced by UV laser irradiation at embryonic stage of Bombyx mori. Int. J. Radiat. Biol. 2006; 82: 648-657.

7. Hosagoudar SR, Manjunatha HB. Picosecond UV laser induced morphological, biochemical and biological changes in Bombyx mori. Iran. J. Radiat. Res. 2011; 9: 127-137.

8. Walsh DA, Klein NW, Hightower LE, Edwards MJ. Heat shock and thermotolerance during early rat embryo development. Teratology. 1987; 36: 181-191.

9. Potter KA, Davidowitz G, Woods HA. Cross-stage consequences of egg temperature in the insect Manduca sexta. Funct. Ecol. 2010; 1365: 2435.

10. Woods HA, Bonnecaze RT. Insect eggs at a transition between diffusion and reaction limitation: temperature, oxygen and water. J. Theor. Biol. 2006; 243: 483-492.

11. Narasimhanna MN. Manual on silkworm egg production. Central silk board, Bangalore. 1988; 110-111.

12. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem.1951; 193: 265-275.

13. Weber K, Osborn J. The reliability of molecular weight determination by dodecyl sulphate-polyacrylamide gel electrophoresis. J. Biol. Chem. 1969; 244: 4406-4412.

14. Vasudha BC, Aparna HS, Manjunatha HB. Impact of heat shock on heat shock proteins expression, biological and commercial traits of Bombyx mori. Insect Sci. 2006; 13: 243-250.

15. Fan L, Lin J, Zhong Y, Liu J. Shotgun proteomic analysis on the diapause and non-diapause eggs of domesticated silkworm Bombyx mori. PLoS ONE 2013; 4:e60386. doi:10.1371/journal.pone.0060386.

16. Suresh Kumar N, Harjeet Singh. Evaluation of the reproductive potential of bivoltine silkworm hybrids of Bombyx mori L under high temperature and high humidity and high temperature and low humidity conditions of the tropics. Univ. J. Environ. Res. Tech. 2012; 2: 443-449.

17. Manjunatha HB, Zamood A, Vasudha BC, Aparna HS. Heat shock response and analysis of egg proteins in new bivoltine strains of Bombyx mori. Sericologia. 2005; 45:403-408.

18. Coulon BΜ, Mathelin J. Variations in the rate of synthesis of heat shock proteins HSP70, between laying and neurula, the diapausing embryo of the silkworm Bombyx mori. Sericologia. 1991; 31: 295–300.

19. Welte MA, Tetrault JM, Dellavalle RP, Lindquist S. A new method for manipulating Trans genes: engineering heat tolerance in a complex, multi-cellular organism. Curr. Biol. 1993; 3: 842-853.

20. Ebrahimi N, Talebi AA, Fathipour Y. Effects of short-term heat shock of eggs on the development and fecundity of Plutella xylostella (L.) (Lepidoptera: Plutellidae). J. Crop Prot. 2015; 1: 73-83.

21. Aparna HS, Kundapur RR, Manjunatha HB. Molecular characterization of heat shock proteins 90 (HSP83?) and 70 in tropical strains of Bombyx mori. Proteomics 2010; 10: 2734-2745.

22. Liu WW, Yang P, Chen XM, Xu DL, Hu YH. Cloning and expression analysis of four heat shock protein gene in Ericrus plea (Homoptera: Coccidae), J. Insec. Sci. 2014; 14: 142.

23. Li ZW, Li X, Yu QY, Xiang ZH, Kishino H, Zhang Z. The small heat shock protein (sHSP) genes in the silkworm, Bombyx mori, and comparative analysis with other insect sHSP genes. BMC Evol. Biol 2009; 9:215.

24. Sasibhushan S, Ponnuvel KM, Vijayaprakash NB. Diapause specific gene expression in the eggs of multivoltine silkworm Bombyx mori, identified by suppressive subtractive hybridization. Comp. Biochem. Physiol. 2012; B 161:371-79.

25. Joy O, Gopinathan KP. Heat shock response in mulberry silkworm races with different thermotolerances, J. Biosci. 1995; 20: 499–513.

26. Taha RH. Impact of thermal stress on the haemolymphal proteins, biological and economical characters of the silkworm, Bombyx mori L. Egypt. Acad. J. Biolog. Sci. 2013; 1: 113-122.

27. Dohino T, Mizuno T, Mizuniwa S, Yoneda M, Miyazaki I. Heat and cold tolerance of various aged eggs of Bactrocera dorsalis and B. cucurbitae (Diptera: Tephritidae). Res. Bull. Pl. Prot. Japan. 2014; 50: 63-69.

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