Research Article | Volume: 5, Issue: 1, Jan-Feb, 2017
Control of Fusarium oxysporum infection in transgenic tobacco carrying oxalate descarboxilase gene
Fusarium oxysporum is a widespread necrotrophic plant pathogen. Its infection affects several crop plants such as potato, tomato, cotton, banana, coffee, strawberry and sugarcane. The fungus produces oxalic acid, which triggers apoptosis in plants by a reactive oxygen species-dependent mechanism. Tobacco plants were genetically modified to express a gene coding for the enzyme oxalate descarboxilase (OxDc), isolated from Flammulina velutipes. The transgenic plants showed tolerance to Fusarium oxysporum. Even under lower expression of the gene, plants were able to control F. oxysporum infection. Thus, we demonstrated that the expression of the oxdc gene is a good alternative for the development of F. oxysporum resistant crops.
Amaral DLAS, Pinto NA, Souza VC, Aragão FJL, Santos MO. Control of Fusarium oxysporum infection in transgenic tobacco carrying oxalate descarboxilase gene. J App Biol Biotech. 2017; 5 (01): 079-083. DOI: 10.7324/JABB.2017.50114
1. Grover A, Gowthaman R. Strategies for development of fungus-resistant transgenic plants. Current Science. 2003, 84: 330-340.
2. Garcíaâ€Guzmán, G, Heil M. Life histories of hosts and pathogens predict patterns in tropical fungal plant diseases. New Phytologist. 2014, 201:1106-1120.
3. Koike ST, Gordon TR. Management of Fusarium wilt of strawberry. Crop Protection. 2015, 73:67-72.
4. Pietro AD, Madrid MP, Caracuel Z, Delgadoâ€Jarana J, Roncero MIG. Fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. Molecular Plant Pathology. 2003, 4(5):315-325.
5. Favaron F, Sella L, D'ovidio R. Relationships among endopolygalacturonase, oxalate, pH, and plant polygalacturonase-inhibiting protein (PGIP) in the interationc between Sclerotinia sclerotiorum and soybean. Molecular Plant Microbe Interactions. 2004, 17:1402-1409.
6. Cessna SG, Sears VE, Dickman MB, Low PS. Oxalic acid, a pathogenicity factor for Sclerotinia sclerotiorum, suppresses the oxidative burst of the host plant. The Plant Cell Online. 2000; 12(11):2191-2199.
7. Dutton MV, Evans, CS. Oxalate production by fungi: Its role in pathogenicity and ecology in the soil environment. Canadian Journal of Microbiology. 1996, 42:881-895.
8. Guimarães RL, Stotz H. Oxalate production by Sclerotinia sclerotiorum deregulates guard cells during infection. Plant physiology. 2004,136(3):3703-3711.
9. Kim KS, Min JY, Dickman MB. Oxalic acid is an elicitor of plant programmed cell death during Sclerotinia sclerotiorum disease development. Molecular Plant-Microbe Interactions. 2008, 21 (5):605-612.
10. Liang X, Moomaw EW, Rollins JA. Fungal oxalate decarboxylase activity contributes to Sclerotinia sclerotiorum early infection by affecting both compound appressoria development and function. Molecular Plant Pathology. 2015, 16(8):825-836.
11. Silva LF, Dias CV, Cidade LC, Mendes JS, Pirovani CP, Alvim FC, Pereira GAG, Aragão FJL, Cascardo JCM, Costa MG. Expression of an oxalate decarboxylase impairs the necrotic effect induced by Nep1 -like protein (NLP) of Moniliophthora perniciosa in transgenic tobacco. Molecular Plant-Microbe Interactions. 2011, 24:839-848.
12. Mehta A, Datta A. Oxalate decarboxylase from Collybia velutipes: Purification, characterization and cDNA cloning. Journal of Biological Chemistry. 1991, 266:23548-23553.
13. Kesarwani M, Azam K, Natarajan A, Mehta A, Datta A. Oxalate Decarboxylase from Collybia velutipesMolecular cloning and its overexpression to confer resistance to fungal infection in transgenic tobacco and tomato. Journal of Biological Chemistry. 2000, 275:7230-7238.
14. Dias BBA, Cunha WG, Morais LS, Vianna GR, Rech EL, Capdeville GD, Aragão FJL. Expression of an oxalate decarboxylase gene from Flammulina spin transgenic lettuce (Lactuca sativa) plants and resistance to Sclerotinia sclerotiorum. Plant Pathology. 2006, 55:187-193.
15. Cunha WG, Tinoco MLP, Pancoti HL, Ribeiro RE, Aragão FJL. High resistance to Sclerotinia sclerotiorum in transgenic soybean plants transformed to express an oxalate decarboxylase gene. Plant Pathology 2010; 59(4):654-660.
16. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962, 15:473-497.
17. Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 1987, 19:11-15.
18. Dickson MH, Hunter JE. Modification of methods for screening for white mold resistance in greenhouse. Annual Report of the Bean Improvement Cooperative. 1983, 26:85-86.
19. Shaner G, Finney RE. The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology 1977, 67: 1051-1056.
20. Weinhold A, Kallenbach M, Baldwin IT. Progressive 35S promoter methylation increases rapidly during vegetative development in transgenic Nicotiana attenuata plants. BMC Plant Biology, 2013, 13:99.
21. Swarupa V, Ravishankar KV, Rekha A. Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana. Planta. 2014, 239(4):735-751.
22. Liu Z, Luan Y, Li J, Yin Y. Expression of a tomato MYB gene in transgenic tobacco increases resistance to Fusarium oxysporum and Botrytis cinerea. European Journal of Plant Pathology, 2015, 144(3):607-617.
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