Research Article | Volume: 5, Issue: 1, Jan-Feb, 2017

Effect of Artificially Induced Drought Stress on Seedlings of Pinus roxburghii Sarg

Ashish Tewari Amit Mittal   

Open Access   

Published:  Jan 20, 2017

DOI: 10.7324/JABB.2017.50113

Water relation and growth parameters of drought stressed seedlings of Chir pine (Pinus roxburghii Sarg.) were studied over a one year period. The seedlings were subjected to three drought cycles of 9, 18, 27 days and one control level (watered every alternate day) in a polyhouse. Pre-dawn (5.30-6.30 AM) and Mid-day water potential was estimated using a pressure chamber. The bench drying method was followed for developing P-V curves for estimating components of water potential. Osmotic adjustment was estimated as the difference between the osmotic potential at zero and full turgor of seedlings kept at control and those given 27 days drought. Across drought cycles the pre- dawn water potential ranged between -0.59 MPa and -2.12 MPa and the mid-day water potentials were always lower than pre-dawn water potentials. The Osmotic adjustment was – 1.17 MPa at full turgor and -2.0 MPa at zero turgor. The seedlings growth was positively related to soil moisture and water potential. The daily change in water potential (difference between pre-dawn and mid-day water potential) declined with increasing drought and was nominal (less than -0.2 MPa) in seedlings subjected to 18 and 27 days drought cycle. Needle characteristics were severely affected by water stress. In Pinus roxburghii Sarg seedlings osmotic adjustment may be an important adaptive mechanism that assists it to invade and grow on dry inhospitable sites.

Keyword:     Osmotic adjustment Water potential Pinus roxburghii Moisture stress.


Tewari A, Mittal A. Effect of Artificially Induced Drought Stress on Seedlings of Pinus roxburghii Sarg. J App Biol Biotech. 2017; 5 (01): 076-078. DOI: 10.7324/JABB.2017.50113

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Worbes M, Blanchart S, Fichtler E. Relations between water balance, wood traits and phenological behavior of tree species from a tropical dry forest in Costa Rica multi factorial study. Tree Physiology. 2013; 33: 527-53.

2. Arndt S.K, Clifford S.C, Wanek W, Jones H.G, Popp M. Physiological and morphological adaptation of the fruit tree Ziziphus rotundifolia in response to progressive drought stress. Tree Physiology. 2001; 21:705-715.

3. Craine J.M, Engelbrecht B.M.J, Lusk C.H, McDowell N.G, Poorter H. Resource limitation tolerance and the future of ecological plant classification. Front Plant Science. 2012; 3:246,

4. Zobel D.B, Singh S.P. Himalayan Forests and ecological generalization. BioScience. 1997; 47: 35-745.

5. Ralhan P.K, Khanna R.K, Singh S.P, Singh J.S. Phenological characteristics of the tree layer of Kumaun Himalayan forests. Vegetatio. 1985; 60: 91-101.

6. Ralhan P.K, Khanna R.K, Singh S.P. Dynamics of nutrients and leaf mass in central Himalayan trees and shrubs. Ecology. 1987; 68:1974-1983.

7. Singh S.P, Tewari A, Singh S.K, Pathak G.C. Significance of phenologically asynchronous populations of the central Himalayan oaks in drought adaptation. Current Science. 2000; 79(3):353-357

8. Zobel D.B, Singh S.P. Tree water relations along the vegetational gradient in the Himalayas. Current Science. 1995; 68: 742-745.

9. Poudyal K, Jha P.K., Zobel D.B., Thapa C.B. Patterns of leaf conductance and water potential of five Himalayan tree species. Tree Physiology. 2004; 24: 689-699.

10. Shrestha B.B, Jha P.K, Zobel D.B. Water relations and phenology of Pinus roxburghii Sarg. in the Churia hills, central Nepal. International Journal of Ecology & Environmental Science. 2006a; 32 (2):183−192.

11. Teskey R.O, Hinckley T.M. Moisture effects of water stress on trees. In: Henessery TC, Dougherty PM, Kossuth SV, Johnson JD, editors. Stress physiology and forest productivity, Netherlands : M. Nijhoff E-Publishing , Dordrecht; 1986, p.9-33.

12. Vivin P, J.M. Guehl, Clément A, Aussenac G. The effects of elevated CO2 and water stress on whole plant CO2 exchange, carbon allocation, and osmoregulation in oak seedlings. Ann. Sci. For. 1996; 53: 447-459.

13. Pant H, Tewari A. Carbon Sequestration in Chir-Pine (Pinus roxburghii Sarg.) Forests under various disturbance levels in Kumaun Central Himalaya. Journal Forestry Research. 2014; 25 (2): 401-405.

14. Pinto C. A, David J. S, Cochard H, Caldeira M. C, Henriques M. O, Quilho T, Paco T. A, Pereira J. S, David T. S. Drought-induced embolism in current-year shoots of two Mediterranean evergreen oaks. Forest Ecology and Management. 2012; 285:1-10.

15. Bargali K, Tewari A. Growth and water relation parameters in drought-stressed Coriaria nepalensis seedlings. Journal of Arid Environment. 2004; 58: 505-512.

16. Edwards D.R, Dixon M.A. Mechanisms of drought response in Thuja occidentalis L.I: water stress conditioning and osmotic adjustment. Tree Physiology. 1995; 15:121-127.

17. Sharma N, Kumar A, Ram J, Tewari A. Water relation study on seedlings of Quercus leucotrichophora in a greenhouse. Ecology Environment & Conversation. 2001; 7(3):301-305.

18. Snedecor G.W, Cochran W.G. Statistical methods, Oxford and IBH Publishing Co., New Delhi; 1967.

19. Mathson W.J, R.A Haack. The role of drought in outbreaks of plant eating insects. Bioscience. 1987; 37: 110-118.

20. Lopez R, Aranda I, Gil L. Osmotic adjustment is a significant mechanism of drought resistance in Pinus pinaster and Pinus canariensis. Forest Systems. 2009; 18 (2): 159- 166.

21. Bisht K. Growth of Quercus leucotrichophora A. camus and Pinus roxburghii Sarg. seedlings in relation to nutrient and water. Proceedings of Indian National Science Academy. 1993; 59: 71-78.

Article Metrics

152 Absract views 182 PDF Downloads 334 Total views

Related Search

By author names

Citiaion Alert By Google Scholar

Similar Articles