Research Article | Volume: 4, Issue: 6, Nov-Dec, 2016

Recent Advances in substrate utilization for fermentative hydrogen Production

Pankaj K. Rai   

Open Access   

Published:  Nov 05, 2016

DOI: 10.7324/JABB.2016.40608

In many ways, hydrogen is considered to be the ideal fuel for the future. Biohydrogen production processes are considered as the most environmentally friendly in comparison to others. Biological hydrogen production processes (both dark- and photo- fermentation) are considered most favorable. Due to their high conversion efficiency and versatility of the substrate they can utilize. The use of waste materials as substrate not only generates energy but it also helps in the bioremediation. The potential utilization of waste material for H2 production is being investigated extensively. The present review article aims to summarize the recently used substrates for fermentative biohydrogen production.

Keyword:     Biohydrogen waste water food waste algal biomass cellulosic waste.


Rai PK. Recent Advances in substrate utilization for fermentative hydrogen Production. J App Biol Biotec. 2016; 4 (06): 059-067. DOI: 10.7324/JABB.2016.40608

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Das D, Veziroglu TN. Hydrogen production by biological processes: a survey of literature. Int J Hydrogen Energy. 2001; 26: 13-28.

2. Kapdan IK, Kargi F. Bio-hydrogen production from waste materials. Enzy Microbial Technol. 2006; 38: 569-582.

3. Rai PK, Singh SP. Biological production of clean energy: Hydrogen. In: Recent Advances in Microbiology S. P. Tiwari, Rajesh Sharma and Rajeeva Gaur, Editors. Nova Science Publishers Inc. New York, USA; 2013 p. 55-84.

4. Venkata Mohan S. Harnessing of biohydrogen from wastewater treatment using mixed fermentative consortia: process evaluation towards optimization. Int J Hydrogen Energy. 2009; 34: 7460-7474.

5. Wang Y, Wang H, Feng X, Wang X, Huang J. Biohydrogen production from cornstalk wastes by anaerobic fermentation with activated sludge. Int J Hydrogen Energy. 2010; 35: 3092-3099.

6. Fang HHP., Li Chenlin, Zhang Tong. Acidophilic biohydrogen production from rice slurry. Int J Hydrogen Energy. 2006;31:683-692,

7. Seifert K, Waligorska M, Laniecki M. Hydrogen generation in photobiological process from dairy waste water. Int J Hydrogen Energy. 2010; 35: 9624-9629.

8. Eroglu E, Gunduz U, Yucel M, Turker L, Eroglu I. Photobiological hydrogen production from olive mill wastewater as sole substrate sources. Int J Hydrogen Energy. 2004; 29: 163-171.

9. Rai PK, Singh SP, Asthana RK. Biohydrogen Production from cheese whey wastewater in a two-step anaerobic process. Appl Biochem Biotechnol. 2012; 167: 1540-1549.

10. Melis A, Zhang L, Forestier M, Ghirardi ML, Seifert M. Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol. 2000; 122:127-133.

11. Hallenbeck PC, Ghosh D. Advances in fermentative biohydrogen production: the way forward. Trends Biotechnol. 2009; 27: 287-297.

12. Rai PK. Hydrogen production from dairy and agro wastes by integrating dark- and photo- fermentation. Ph.D thesis, Banaras Hindu University, Varanasi-22005, India. 2013.

13. Kim MS, Lee TJ, Yoon YS, Lee IG, Moon KW. Hydrogen production from food processing wastewater and sewage sludge by anaerobic dark fermentation combined with photofermentation. In: Miyake J, Matsunaga T, Pietro AS, editors. Biohydrogen II. Amsterdam: Elsevier 2001; p. 263-272

14. Cappelletti BM, Reginatto V, Amante ER, Antônio RV. Fermentative production of hydrogen from cassava processing wastewater by Clostridium acetobutylicum. Ren Energy. 2011; 36:3367-3372.

15. Rai PK, Singh SP, Asthana RK. Dairy waste based fermentative H2 production. J Microbial World. 2011; 13: 207-213.

16. Rai PK, Singh SP, Asthana RK. Prospects of utilizing dairy waste for biohydrogen production. Int J Biotechnol Biosci. 2011; 1: 263-270.

17. Rai PK, Asthana RK, Singh SP. Optimization of photo- hydrogen production based on cheese whey spent medium. Int J Hydrogen Energy. 2014; 39: 7597-7603.

18. Xiao ND, Chen YG, Chen AH, Feng LY. Enhanced biohydrogen production from protein wastewater by altering protein structure and amino acids acidification type. Scientific Reports. 2014: 4, 3992; DOI: 10.1038/srep03992.

19. Jayalakshmi J, Joseph K, Sukumaran V. Bio hydrogen generation from kitchen waste in an inclined plug flow reactor. Int J Hydrogen Energy. 2009; 34:8854-8858.

20. Hwang JH, Choi JA, Abou-Shanab RAI, Min B, Song H, Kim Y, Lee ES, Jeon BH. Feasibility of hydrogen production from ripened fruits by a combined two-stage (dark/dark) fermentation system. Bioresour Technol. 2011; 102: 1051-1058.

21. Elbeshbishy E, Hafez H, Nakhla G. Ultrasonication for biohydrogen production from food waste. Int J Hydrogen Energy. 2011; 36: 2896-2903.

22. Zhang R, El Mashad HM, Hartman K, Wang F, Liu G, Choate C, Gamble P. Characterization of food waste as feedstock for anaerobic digestion. Bioresourc Technol. 2007; 98: 929-939.

23. Lee DY, Ebie Y, Xu KQ, Li YY, Inamori Y. Continuous H2 and CH4 production from high-solid food waste in the two-stage thermophilic fermentation process with the recirculation of digester sludge. Bioresourc Technol. 2010; 101: 542-547.

24. Kim DH, Kim SH, Shin HS. Hydrogen fermentation of food waste without inoculums addition. Enzyme Microb Technol. 2009; 45:181-87.

25. Kim SH, Shin HS. Effects of base- pretreatment on continuous enriched culture for hydrogen production from food waste. Int J Hydrogen Energy. 2008; 33:5266-5274.

26. Han SK, Shin HS. Biohydrogen production by anaerobic fermentation of food waste. Int J Hydrogen Energy. 2004; 29: 569-577.

27. Vijayaraghavan K, Ahmad D, Ibrahim MK. Biohydrogen generation from jackfruit peel using anaerobic contact filter. Int J Hydrogen Energy. 2006; 31:569-579.

28. Yasin HMN, Noraini AR, Hasfalina CM, Yusoff MZM, Hassan MA. Microbial characterization of hydrogen-producing bacteria in fermented food waste at different pH values. Int J Hydrogen Energy. 2011; 36: 9571-9580.

29. Danko AS, Pinheiro F, Abreu AA, Alves MM. Effect of methanogenic inhibitors, inocula type, and temperature on biohydrogen production from food components. Environ Eng Manage J. 2008; 7:531-536.

30. Elbeshbishy E, Hafez H, Dhar BR, Nakhla G. Single and combined effect of various pretreatment methods for biohydrogen production from food waste. Int J Hydrogen Energy. 2011; 36: 11379-11387.

31. Shin HS, Youn JH, Kim SH. Hydrogen production from food waste in anaerobic mesophilic and thermophilic acidogenesis. Int J Hydrogen Energy. 2004; 29:1355-1363.

32. Chu CF, Li YY, Xu KQ, Ebie Y, Inamori Y, Kong HN. A pH- and temperature-phased two-stage process for hydrogen and methane production from food waste. Int J Hydrogen Energy. 2008;33:4739-46.

33. Kim DH, Kim SH, Jung KW, Kim MS, Shin HS. Effect of initial pH independent of operational pH on hydrogen fermentation of food waste. Bioresour Technol. 2011; 102:8646-8652.

34. Venkata Mohan, Mohanakrishna G, Goud RK, Sarma PN. Acidogenic fermentation of vegetable based market waste to harness biohydrogen with simultaneous stabilization. Bioresourc Technol. 2009; 100: 3061-68.

35. Kim DH, Kim SH, Kim KY, Shin HS. Experience of pilot-scale hydrogen producing anaerobic sequencing batch reactor (ASBR) treating food waste. Int J Hydrogen Energy. 2010; 35:1590-1594.

36. Reddy MV, Chandrasekhar K, Venkata Mohan S. Influence of carbohydrates and proteins concentration on fermentative hydrogen production using canteen based waste under acidophilic microenvironment. J Biotechnol. 2011; 155:387-395.

37. Kumar G, Bakonyi P, Periyasamy S, Kim SH, Nemestóthy N, Bélafi-Bakó K. Lignocellulose biohydrogen: practical challenges and recent progress. Ren Sustain Energy Reviews. 2015; 44, 728-737.

38. Van Wyk JPH, Mohulatsi M. Biodegradation of wastepaper by cellulase from Trichoderma viride. Bioresour Technol. 2003; 86:21-23.

39. Yang H, Guo L, Liu F. Enhanced bio-hydrogen production from corncob by a two-step process: dark- and photo-fermentation. Bioresour Technol. 2010; 101:2049-2052.

40. Sriroth K, Chollakup R, Chotineeranat S, Piyachomkwan K, Christopher GO. Processing of cassava waste for improved biomass utilization. Bioresour Technol. 2000; 71:63-69.

41. Manish S, Banerjee R. Comparison of biohydrogen production processes. Int J Hydrog Energy. 2008; 33:279-286.

42. Phowan P, Danvirutai P. Hydrogen production from cassava pulp hydrolysate by mixed seed cultures: Effects of initial pH, substrate and biomass concentrations. Biomass Bioenergy. 2014; 64; 1-10.

43. Cheng J, Su H, Zhou J, Song W, Cen K. Hydrogen production by mixed bacteria through dark and photo fermentation. Int J Hydrogen Energy. 2011; 36: 450-457,

44. Rai PK, Singh SP, Asthana RK. Biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation. Bioresour Technol. 2014; 152:140-146.

45. Kumar G, Sivagurunathan P, Chen Chin-Chao, Lin Chiu-Yue. Batch and continuous biogenic hydrogen fermentation of acid pretreated de-oiled Jatropha waste (DJW) hydrolysate. RSC Adv. 2016; 6:45482-91.

46. Kumar G, Sen B, Sivagurunathan P, Lin Chiu-Yue. High rate hydrogen fermentation of cello-lignin fraction in de-oiled jatropha waste using hybrid immobilized cell system. Fuel 2016; 182:131-140.

47. Kawaguchi H, Hashimoto K, Hirata K, Miyamoto K. H2 production from algal biomass by a mixed culture of Rhodobium marinum A-501 and Lactobacillus amylovorus. J Biosci Bioeng. 2001; 91:277-282.

48. Kumar K, Roy S, Das D. Continuous mode of carbon dioxide sequestration by C. sorokiniana and subsequent use of its biomass for hydrogen production by E. cloacae IIT-BT 08.Bioresour Technol. 2013;145:116-122.

49. Roy S, Kumar K, Ghosh S, Das D. Thermophilic biohydrogen production using pre- treated algal biomass as substrate. Biomass Bioenergy. 2014; 61:157-166.

50. Nguyen MT, Choi SP, Lee J, Lee JH, Sim SJ. Hydrothermal acid pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. J Microbiol Biotechnol. 2009; 19:161-166.

51. Nguyen TAD, Kim KR, Nguyen MT, Kim MS, Kim D, Sim SJ. Enhancement of fermentative hydrogen production from green algal biomass of Thermotoga neapolitana by various pretreatment methods. Int J Hydrogen Energy. 2010; 35:13035-13040.

52. Park JH, Yoon JJ, Park HD, Kim YJ, Lim DJ, Kim SH. Feasibility of biohydrogen production from Gelidium amansii. Int J Hydrogen Energy. 2011; 36:13997-14003.

53. Jung KW, Kim DH, Shin HS. Fermentative hydrogen production from Laminaria japonica and optimization of thermal pretreatment conditions. Bioresour Technol. 2011; 102:2745-2750.

54. Jung KW, Kim DH, Kim HW, Shin HS. Optimization of combined (acid + thermal) pretreatment for fermentative hydrogen production from Laminaria japonica using response surface methodology (RSM). Int J Hydrogen Energy. 2011; 36: 9626-9631.

55. Liu CH, Chang CY, Cheng CL, Lee DJ, Chang JS. Fermentative hydrogen production by Clostridium butyricum CGS5 using carbohydrate-rich microalgal biomass as feedstock. Int J Hydrogen Energy. 2012; 37: 15458-15464.

56. Park JH, Cheon HC, Yoon JJ, Park HD, Kim SH. Optimization of batch dilute-acid hydrolysis for biohydrogen production from red algal biomass. Int J Hydrogen Energy. 2013; 38: 6130-6136.

57. Liu H, Wang G. Fermentative hydrogen production from macroalgae Laminaria japonica using anaerobic mixed Bacteria. Int J Hydrogen Energy. 2014; 39: 9012-9017.

58. Nayak BK, Roy S, Das D. Biohydrogen production from algal biomass (Anabaena sp. PCC 7120) cultivated in airlift photobioreactor. Int J Hydrogen Energy. 2014; 39:7553-7560.

59. Subhash G and Venkata Mohan SV. Deoiled algal cake as feedstock for dark fermentative biohydrogen production: An integrated biorefinery approach. Int J Hydrogen Energy. 2014; 39: 9573-9579.

60. Ferchichi M, Crabbe E, Hintz W, Gill GH, Almadidy A. Influence of culture parameters on biological hydrogen production by Clostridium saccharoperbutylacetonium ATCC 27021. World J Microbiol Biotechnol. 2005; 21: 855-862.

61. Yang P, Zhang R, McGarvey JA, Benemann JR. Biohydrogen production from cheese processing wastewater by anaerobic fermentation using mixed microbial communities. Int J Hydrogen Energy. 2007; 32: 4761-4771.

62. Azbar N, Dokgöz FTC, Keskin T, Korkmaz KS, Syed HM. Continuous fermentative hydrogen production from cheese whey wastewater under thermophilic anaerobic conditions. Int J Hydrogen Energy. 2009; 34:7441-7447.

63. Oh SE, Logan BE. Hydrogen and electricity production from a food processing wastewater using fermentation and microbial fuel cell technologies. Water Res. 2005; 39: 4673-4682.

64. Davila-Vazquez G, Cota-Navarro CB, Rosales-Colunga LM, León-Rodríguez A, Razo- Flores E. Continuous biohydrogen production using cheese whey: improving the hydrogen production rate. Int J Hydrogen Energy. 2009; 34:4296-4304.

65. Amorim NCS, Alves I, Martins JS, Amorim ELC. Biohydrogen Production from Cassava Wastewater in an Anaerobic Fluidized Bed Reactor. Brazilian J Chem Eng. 2014; 31:603-612.

66. Han SK, Shin HS. Biohydrogen production by anaerobic fermentation of food waste. Int J Hydrogen Energy. 2004; 29:569-577.

67. Wang CH, Lin PJ, Chang JS. Fermentative conversion of sucrose and pineapple waste into hydrogen gas in phosphate-buffered culture seeded with municipal sewage sludge. Process Biochem. 2006; 41:1353-1358.

68. Doi T, Matsumoto H, Abe J, Morita S. Feasibility study on the application of rhizosphere microflora of rice for the biohydrogen production from wasted bread. Int J Hydrogen Energy. 2009; 34: 1735-1743.

69. Li SL, Lin JS, Wang YH, Lee ZK, Kuo SC, Tseng IC, Cheng SS. Strategy of controlling the volumetric loading rate to promote hydrogen-production performance in mesophilic-kitchen-waste fermentor and the microbial ecology analyses. Bioresourc Technol. 2011; 102:8682-8687.

70. Pattra S, Sangyoka S, Boonmee M, Reungsang A. Biohydrogen production from the fermentation of sugarcane bagasse hydrolysate by Clostridium butyricum. Int J Hydrogen Energy. 2008; 33:6058-6065.

71. Panagiotopoulos IA, Bakker RR, De Vrije T, Koukios EG, Classen PAM. Pretreatment of sweet sorghum bagasse for hydrogen production by Caldi-cellulosiruptor saccharolyticus. Int J Hydrogen Energy. 2010; 35:7738-7747.

72. Wang Y, Wang H, Feng X, Wang X, Huang J. Biohydrogen production from cornstalk wastes by anaerobic fermentation with activated sludge. Int J Hydrogen Energy. 2010; 35:3092-3099.

73. Nasirian N, Almassi M, Minaei S, Widmann R. Development of a method for biohydrogen production from wheat straw by dark fermentation. Int J Hydrogen Energy. 2011; 36:411-420.

74. Cui M and Shen J. Effects of acid and alkaline pretreatments on the biohydrogen production from grass by anaerobic dark fermentation. Int J Hydrogen Energy. 2012; 37:1120-1124.

75. Han H, Wei L, Liu B, Yang H, Shen J. Optimization of biohydrogen production from soybean straw using anaerobic mixed bacteria. Int J Hydrogen Energy. 2012; 37:13200-13208.

76. Lay CH, Sen B, Chen CC, Wu JH, Lee SC, Lin CY. Co-fermentation of water hyacinth and beverage wastewater in powder and pellet form for hydrogen production. Bioresour Technol. 2013; 135:610-615.

77. Zhao L, Cao GL, Wang AJ, Guo WQ, Ren HY, Ren NQ. Simultaneous saccharification and fermentation of fungal pre-treated cornstalk for hydrogen production using Thermoanaerobacterium thermosaccharolyticum W16. Bioresour Technol. 2013; 145:103-107.

78. Song ZX, Li XH, Li WW, Bai YX, Fan YT, Hou HW. Direct bioconversion of raw corn stalk to hydrogen by a new strain Clostridium sp. FS3. Bioresour Technol. 2014; 157:91-97.

79. Shi X, Jung KW, Kim DH, Ahn YT, Shin HS. Direct fermentation of Laminaria japonica for biohydrogen production by anaerobic mixed cultures. Int J Hydrogen Energy. 2011; 36:5857-5864.

80. Ho KL, Lee DJ, Su A, Chang JS. Biohydrogen from lignocellulosic feedstock via one-step process. Int J Hydrogen Energy. 2012; 37: 15569-74.

81. Yun YM, Jung KW, Kim DH, Oh YK, Shin HS. Microalgal biomass as a feedstock for biohydrogen production. Int J Hydrogen Energy. 2012; 37:15533-15539.

82. Ferreira AF, Marques AC, Batista AP, PASS Marques, Gouveia L, Silva CM. Biological hydrogen production by Anabaena sp – yield, energy and CO2 analysis including fermentative biomass recovery. Int J Hydrogen Energy. 2012; 37:179-190.

83. Cheng J, Xia A, Liu Y, Lin R, Zhou J, Cen K. Combination of dark- and photo-fermentation to improve hydrogen production from Arthrospira platensis wet biomass with ammonium removal by zeolite. Int J Hydrogen Energy. 2012; 37: 13330-13337.

84. Xia A, Cheng J, Lin R, Lu H, Zhou J, Cen K. Comparison in dark hydrogen fermentation followed by photo hydrogen fermentation and methanogenesis between protein and carbohydrate compositions in Nannochloropsis oceanica biomass. Bioresour Technol. 2013; 138:204-213.

85. Shi X, Kim DH, Shin HS, Jung KW. Effect of temperature on continuous fermentative hydrogen production from Laminaria japonica by anaerobic mixed cultures. Bioresour Technol. 2013; 144:225-231.

86. Batista AP, Moura P, Marques PASS, Ortigueira J, Alves L, Gouveia L. Scenedesmus obliquus as feedstock for biohydrogen production by Enterobacter aerogenes and Clostridium butyricum. Fuel. 2014; 117:537-543.

Article Metrics

170 Absract views 166 PDF Downloads 336 Total views

Related Search

By author names

Citiaion Alert By Google Scholar