Characterization of a pollen-specific agp1-like protein in Arabidopsis thaliana

Tian Wu; Feng Feng; Changhui Ye; Yingzhi Li

doi:10.7324/JABB.2016.40602

Abstract References Article Metrics Similar Articles Request Permission Related Search Citation Alert By Google Scholar Comment On This Article

Abstract

In plants, pollen tube germination occurs widely in flowering plants. In Arabidopsis thaliana, it has been reported ARABINOGALACTAN PROTEIN 1 (AGP1) plays an important role in pollen tube germination. The expression of arabinogalactan protein in Arabidopsis pollen tubes has been extensively studied. Herein, we characterized an Arabidopsis AGP1-LIKE PROTEIN (ALP), which is shown to have 47% homology at the amino acid level to At1g24520.1 (BCP1), POLLEN PROTEIN1 in Brassica campestris. BCP1, which is highly expressed in both tapetum and microspores, is essential for pollen fertility. Transgenic Arabidopsis transformed with an ALP promoter-driven β-glucuronidase (GUS) construct exhibited strong GUS activity in the pollen and young siliques, in good agreement with the RT-qPCR analysis. To further understand the function of ALP, the ALP-RNAi lines were generated for further investigations. Phenotypic deficiency of siliques was observed in the ALP-RNAi line, in comparison to the wild type. Furthermore, scanning electron microscopy suggested defects in the ALP-RNAi line and in vivo pollen germination showed reduced ability in the ALP-RNAi line. Taken together, our results suggested the important role of ALP in pollen and seed development.

Keyword: agp1-like protein Arabidopsis development pollen pollen tube siliques

Citation:

Wu T, Feng F, Ye C, Li Y. Characterization of a pollen-specific agp1-like protein in Arabidopsis thalian. J App Biol Biotech. 2016; 4 (06): 010-014. DOI: 10.7324/JABB.2016.40602

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

Reference

1. Borg M, Brownfield L, Twell D. Male gametophyte development: a molecular perspective. J Exp Bot.2009; 60: 1465-1478.

2. Piffanelli P, Ross JH, Murphy D. Biogenesis and function of the lipidic structures of pollen grains. Sex Plant Reprod. 1998; 11: 65-80.

3. Piffanelli P, Ross JH, Murphy DJ. Intra- and extracellular lipid composition and associated gene expression patterns during pollen development in Brassica napus. Plant J. 1997; 11: 549-562.

4. Pina C, Pinto F, Feijo JA, Becker JD. Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation. Plant Physiol. 2005; 138: 744-756.

5. Quilichini TD, Friedmann MC, Samuels AL, Douglas CJ. ATP-binding cassette transporter G26 is required for male fertility and pollen exine formation in Arabidopsis. Plant Physiol. 2010; 154: 678-690.

6. Franklin-Tong VE. Signaling in pollination. Curr Opin Plant Biol. 1999; 2: 490-495.

7. Gu F, Nielsen E. Targeting and regulation of cell wall synthesis during tip growth in plants. J Integr Plant Biol. 2013; 55: 835-846.

8. Guan Y, Guo J, Li H, Yang Z. Signaling in pollen tube growth: crosstalk, feedback, and missing links. Mol Plant. 2013; 6: 1053-1064.

9. Farmer EE, Weber H, Vollenweider S. Fatty acid signaling in Arabidopsis. Planta. 1998; 206: 167-174.

10. Wang X. Lipid signaling. Curr Opin Plant Biol. 2004; 7: 329-336.

11. Aarts MG, Keijzer CJ, Stiekema WJ, Pereira A. Molecular characterization of the CER1 gene of arabidopsis involved in epicuticular wax biosynthesis and pollen fertility. PlantCell. 1995; 7: 2115-2127.

12. Coimbra S, Almeida J, Junqueira V, Costa ML, Pereira LG.Arabinogalactan proteins as molecular markers in Arabidopsis thaliana sexual reproduction. J Exp Bot. 2007; 58: 4027-4035.

13. Coimbra S, Jones B, Pereira LG. Arabinogalactan proteins (AGPs) related to pollen tube guidance into the embryo sac in Arabidopsis. Plant Signal Behav. 2008; 3: 455-456.

14. Seifert GJ, Roberts K. The biology of arabinogalactan proteins. Annu Rev Plant Biol. 2007; 58: 137-161.

15. Pereira AM, Masiero S, Nobre MS, Costa ML, Solis MT, Testillano PS, et al. Differential expression patterns of arabinogalactan proteins in Arabidopsis thaliana reproductive tissues. J Exp Bot.2017; 65: 5459-5471.

16. Willats W, Knox J. A role for arabinogalactanâ€proteins in plant cell expansion: evidence from studies on the interaction of βâ€glucosyl Yariv reagent with seedlings of Arabidopsis thaliana. Plant J.1996; 9: 919-925.

17. Majewska-Sawka A, Nothnagel E. The multiple roles of arabinogalactan proteins in plant development. Plant Physiol.2000; 122: 3-10.

18. Xu H, Knox RB, Taylor PE, Singh MB. Bcp1, a gene required for male fertility in Arabidopsis. Proc Natl Acad Sci USA 1995; 92: 2106-2110.

19. Theerakulpisut P, Xu H, Singh MB, Pettitt JM, Knox RB. Isolation and developmental expression of Bcp1, an anther-specific cDNA clone in Brassica campestris. Plant Cell. 1991; 3: 1073-1084.

20. Clough SJ, Bent AF. 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1998;16: 735-743.

21. Jefferson RA, Kavanagh TA, Bevan MW. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987; 6: 3901-3907.

22. Taylor LP, Hepler PK. Pollen germination and tube growth. Annu.Rev.Plant Biol.1997; 48: 461-491.

23. Wilson ZA, Zhang DB. From Arabidopsis to rice: pathways in pollen development. J Exp Bot. 2009; 60: 1479-1492.

24. Ariizumi T, Toriyama K. Genetic regulation of sporopollenin synthesis and pollen exine development. Annu Rev Plant Biol. 2001; 62: 437-460.

25. Becker JD, Boavida LC, Carneiro J, Haury M, Feijo JA. Transcriptional profiling of Arabidopsis tissues reveals the unique characteristics of the pollen transcriptome. Plant Physiol. 2003; 133: 713-725.

26. da Costa-Nunes JA, Grossniklaus U. Unveiling the gene-expression profile of pollen. Genome Biol. 2003; 5: 205.

27. Hirano K, Aya K, Hobo T, Sakakibara H, Kojima M, Shim RA, et al. Comprehensive transcriptome analysis of phytohormone biosynthesis and signaling genes in microspore/pollen and tapetum of rice. Plant Cell Physiol. 2008; 49: 1429-1450.

28. Mascarenhas JP. The Biochemistry of Angiosperm Pollen Development. Botanical Review. 1975; 41: 259-314.

29. Evans DE, Taylor PE, Singh MB, Knox RB. The interrelationship between the accumulation of lipids, protein and the level of acyl carrier protein during the development of Brassica napus L. pollen. Planta. 1992; 186: 343-354.

30. Johnson-Brousseau SA, McCormick S. A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically-expressed genes. Plant J. 2004; 39: 761-775.

31. McCormick S, Twell D, Vancanneyt G, Yamaguchi J. Molecular analysis of gene regulation and function during male gametophyte development. Symp Soc Exp Biol. 1991; 45: 229-244.

32. Dobritsa AA, Lei Z, Nishikawa S, Urbanczyk-Wochniak E, Huhman DV, Preuss D et al. LAP5 and LAP6 encode anther-specific proteins with similarity to chalcone synthase essential for pollen exine development in Arabidopsis. Plant Physiol. 2010; 153: 937-955.

Article Metrics

100 Views 50 Downloads 150 Total

Year

Month

Related Search

By author names

Wu T [PubMed] [Google Scholar ]

Feng F [PubMed] [Google Scholar ]

Ye C [PubMed] [Google Scholar ]

Li Y [PubMed] [Google Scholar ]

By article title