Research Article | Volume: 4, Issue: 6, Nov-Dec, 2016

Molecular Phylogeny of Balsams (Genus Impatiens) Based on ITS Regions of Nuclear Ribosomal DNA Implies Two Colonization Events in South India

P. P. Shajitha N. R. Dhanesh P. J. Ebin Laly Joseph Aneesha Devassy Reshma John Jomy Augustine Linu Mathew   

Open Access   

Published:  Nov 05, 2016

DOI: 10.7324/JABB.2016.40601

The present investigation is the first attempt of its kind to analyze the phylogenetic association of Impatiens species of South India by placing them in the global Internal Transcribed Spacer (ITS) phylogeny of Impatiens. Representative samples from all the seven sections of genus Impatiens reported from South India were collected. Total genomic DNA was extracted from fresh plant leaf and Polymerise Chain Reaction (PCR) was carried out using ITS specific forward and reverse primers. Forty seven sequences from representatives of seven sections of Impatiens species from South India and 45 Impatiens sequences from other centers of Impatiens diversity were obtained from GenBank and used to reconstruct the evolutionary relationships within the genus Impatiens. Maximum Parsimony (MP) analysis was conducted by using PAUP*v.4.0b10. Bayesian Inference (BI) analysis was carried out in MrBayes v.3.2.2. Molecular phylogeny of South Indian Impatiens spp. based on ITS sequences showed the same association as that of morphological taxonomy. This study including all representative extant sections of South India, indicates that South India was colonized from Southeast Asia by two independent dispersal events i.e., once by ancestral species of sections Scapigerae, Epiphyticae, Tomentosae, Sub-Umbellatae and Racemosae and another time by ancestral species of sections Microsepalae and Annuae.

Keyword:     Impatiens species Molecular Phylogeny Internal Transcribed Spacer Maximum Parsimony Bayesian Inference.


Shajitha PP, Dhanesh NR, Ebin PJ, Joseph L, Devassy A, John R, Augustine J, Mathew L. Molecular Phylogeny of Balsams (Genus Impatiens) Based on ITS Regions of Nuclear Ribosomal DNA Implies Two Colonization Events in South India. J App Biol Biotech. 2016; 4 (06): 001-009. DOI: 10.7324/JABB.2016.40601

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Janssens S, Geuten K, Yuan YM, Song Y, Kupfer P, Smets E. Phylogenetics of Impatiens and Hydrocera (Balsaminaceae) using chloroplast atpB-rbcL spacer sequences. Systematic Botany. 2006; 31(1):171-180.

2. Yuan YM, Song YI, Geuten K, Rahelivololona E, Wohlhauser S, Fischer E, Kupfer P. Phylogeny and biogeography of Balsaminaceae inferred from ITS sequences. Taxon. 2004; 53(2):391-391.

3. Grey-Wilson C. Impatiens in Papuasia, Studies in Balsaminaceae. Kew Bull. 1980a; 34: 661-688.

4. Rao R, Ayyangar K, Sampathkumar R. On the karyological characteristics of some members of Balsaminaceae. Cytologia. 1986; 51:251-260.

5. Bhaskar V. Taxonomic Monograph on Impatiens L. (Balsaminaceae) of Western Ghats, South India: The Key Genus for Endemism. Karnataka, India: Centre for Plant Taxonomic Studies; 2012.

6. Kuang RP, Duan LD, Gu JZ, Cai XZ, Cong YY, Liu KM. Impatiens liboensis sp. nov. (Balsaminaceae) from Guizhou, China. Nordic Journal of Botany. 2014; 32(4):463-467.

7. Luo Q, Wang TJ, Zhao LH. Impatiens shimianensis sp. nov. (Balsaminaceae) from Sichuan, China. Nordic Journal of Botany. 2015; 33(2):204-208.

8. Gogoi R, Borah S. Impatiens paramjitiana, a new species of Balsaminaceae from Arunachal Pradesh, India. Phytotaxa. 2014; 175(3):171-175.

9. Jones K, Smith J. The cytogeography of Impatiens L. (Balsaminaceae). Kew Bulletin. 1966; 20:63-72.

10. Grey-Wilson C. Impatiens of Africa, Rotterdam Balkema. Kew Bulletin. 1980b; 34:221-227.

11. Bhaskar V. The genus Impatiens L. in south India: endemism and affinities. Indian Forester. 1981; 107:368-376.

12. Yu SX, Janssens SB, Zhu XY, Lidén M, Gao TG, Wang W. Phylogeny of Impatiens (Balsaminaceae): integrating molecular and morphological evidence into a new classification. Cladistics. 2015; 1-19.

13. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (ed) PCR Protocols: A Guide to Methods and Applications. Academic Press, New York. 1990; 315-322.

14. Thomson JD, Higgins DG, Gibson TJ. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position- specific gap penalties and weight matrix choice. Nucleic Acids Research. 1994; 22:4673-4680.

15. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and program for Windows 95/98/NT. In Nucleic acids symposium series. 1999; 41:95-98.

16. Swofford DL. PAUP* version 4.0b10. Phylogenetic analysis using patsimony (*and other methods), Sinauer, Sunderland, MA. 2002.

17. Darriba D, Taboada GL, Doallo R, Posada D. jModelTest 2: more models, new heuristics and parallel computing. Nature methods. 2012; 9(8):772-772.

18. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A. MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice across a Large Model Space. Systematic Biology. 2012; 61(3):539-42. doi:10.1093/ sysbio/sys029. 4.

19. Rambaut A. FigTree. v. 1.4. 2: tree drawing tool. 2014.

20. Janssens, SB, Knox EB, Huysmans S, Smets EF, Merckx VS. Rapid radiation of Impatiens (Balsaminaceae) during Pliocene and Pleistocene: result of a global climate change. Molecular Phylogenetics and Evolution. 2009; 52(3), 806-824.

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