Research Article | Volume: 4, Issue: 5, Sep-Oct, 2016

Effects of Vitex doniana leaf extract on aluminium-induced toxicity in male albino Wistar rats

O. E. Yakubu ,  O. F. C. Nwodo,  C. Imo,  M. Abdulrahaman,  L. B. Uyeh   
O. E. Yakubu1 2, O. F. C. Nwodo2, C. Imo1, M. Abdulrahaman3, L. B. Uyeh4

1Department of Biochemistry, Federal University Wukari, Nigeria.

2Department of Biochemistry, University of Nigeria, Nsukska, Nigeria.

3Department of Microbiology, Kogi State University,Anyigba Nigeria.

4Department of Medical Biochemistry, Cross River University of Technology, Calabar, Nigeria.

Open Access   

Published:  Oct 23, 2016

DOI: 10.7324/JABB.2016.40506
PDF [ 0.3 MB]
Request Permission
Share
Download Citation
Print
Download PDF
Abstract

This study was conducted to determine the effect of ethanolic leaf extract of Vitex doniana on the toxicity induced by aluminium chloride in male Wistar albino rats. A total of 15 male albino rats were used for the study and toxicity was induced using 100mg/kg body weight (bw) AlCl3. They were grouped into three (five rats in each group): normal control, AlCl3 control and AlCl3 + 100mg Vitex doniana respectively. The normal control group was fed with standard rat chow only, while the AlCl3 control group was orally administered 100mg/kgbw AlCl3, and the AlCl3 + V.D group was orally administered 100mg/kgbw extract following administration of AlCl3. The study was conducted for 7 days after which the effect of the extract was assessed following the animal sacrifice. Oral dose of 100mg/kg bw extract of Vitex doniana did not show any significant change in TBARS level of liver and testes in the experimental groups, while the AST, ALT and ALP levels in AlCl3 control and AlCl3 + 100mg Vitex doniana showed significant increase (p<0.05) and non-significant increase (except ALT which increased significantly) with control group respectively. The PCV and Hb levels did not show any significant (p>0.05) change from the control group, while there was no significant difference in bilirubin level among the different experimental groups. More research should therefore be done on this study to further understand the usefulness of ethanolic leaf extract of Vitex doniana, and encourage its application in herbal medicine.


Keyword:     Vitex doniana Aluminum Chloride toxicity Albino rats ethanolic extract.

Citation:

Yakubu, O. E., Nwodo, O. F. C., Abdulrahaman, M. and Uyeh, L. B. Effects of Vitex doniana leaf extract on aluminium-induced toxicity in male albino Wistar rats. J App Biol Biotech. 2016; 4 (05): 037-040. DOI: 10.7324/JABB.2016.40506

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.
HTML Full Text
Reference

1. Lira R, Casas A, Rosas-López R, Paredes-Flores M, Pérez-Negrón E, RangelL- Landa S, Solís L, Torres I, Dávila P. Traditional knowledge and useful plant richness in the Tehuacán-Cuicatlán Valley, Mexico, Econ. Bot. 2009; 63:271-287.

2. Mirutse G, Zemeda A, Thomas E, Zerihum W. An ethnobotanical study of medicinal plants used by the Zay people in Ethiopia, J Ethnopharmacol. 2003; 85:43-52.

3. Sofowora AE. The state of medicinal plant research in Nigeria, Ibadan University Press, Nigeria, 1993; pp. 283.

4. Gilani AH, Rahman AU. Trends in ethnopharmacology. J ofEthopharmacol; 2005;100:43-49.

5. Babalola EO. The persistence of African Traditional medicine in contemporary Nigeria Society. AfricanMarburgensia. 1993;26:4.

6. Ladeji O, Okoye ZSC. Anti-hepatotoxic properties of Vitex doniana bark extract. Pharm. Biol. 1996;34(5):355-358.

7. Shahraki MR, Zahedi AS, Sarkaki AR. The Effect of Aluminum Injection in Lateral Ventricle on Sex Hormones in Male Rat, Shiraz E-Medical Journal . 2004; 5:1-10.

8. Yousef MI, and Salama AF. Propolis protection from reproductive toxicity caused by aluminium chloride in male rats. Food and Chemical Toxicology. 2009; 47:1168-1175.

9. Guo CH, Lin CY, Yeh MS, Hsu GW. Aluminum-induced suppression of testosterone through nitric oxide production in male mice, Environment Toxicology Pharmacology. 2005; 19:33-40.

10. Reitman S, Frankel S. Amer. J. Clin. Path. 1957. 28:56.

11. Jendrassik L, Gróf P. Vereinfachte photometrische Methoden zur Bestimmung des Blutbilirubins. Biochem Zeitschrift;1938; 297:82-9.

12. Turner T, Lysiak JJ. Oxidative Stress: A Common Factor in Testicular Dysfunction. Journal of Andrology. 2008; 29: 488-498.

13. Hoffman D. MedicalHerbalism: The Science and Practice of Herbal Medicine. Rochester, VT: Healing Arts Press. 2003;13:62-120.

14. Hala AH, Inas ZA,Gehan MK. Grape seed extract alleviate reproductive toxicity caused by aluminium chloride in male rats. Journal of American Science. 2010; 6(12):1201-1204.

15. Türkez H, GeyikoÄŸlu F, Çolak S. The protective effect of boric acid on aluminum-inducedhepatotoxicity and genotoxicity in rats. Turk J Biol. 2011; 35: 293-301.

16. Christie S, Walker A. Vitex agnus-castus L. (1) A review of its traditional and modern therapeutic use; (2) current use from a survey of practitioners. Eur J Herbal Med. 1999; 3: 29-45.

17. Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes, Oxidative Medicine and Cellular Longevity. 2008;1:15-24.

Article Metrics
139 Views 45 Downloads 184 Total

Year

Month

Related Search

By author names