Research Article | Volume: 4, Issue: 4, July-August, 2016

Bioconversion of waste newspaper into fermentable sugars at different temperatures with different Aspergillus niger cellulase concentrations

J Boitumelo M Sibiya J Pieter H van Wyk   

Open Access   

Published:  Aug 21, 2016

DOI: 10.7324/JABB.2016.40408

Used newspaper is a major component of wastepaper that forms part of waste cellulosic materials which exhibits the potential to be developed as a resource of bio-energy. An initial step in utilizing the bio-energy potential of used newspaper is to bioconvert the cellulose compound into fermentable sugars such as glucose. This step can be performed with a hydrolytic enzyme such as cellulase from A. niger. Various amounts of used wastepaper have been treated with different concentrations of cellulase from A. niger at different incubation temperatures. Optimum degradation was observed at 50°C with the highest cellulase concentration and during degradation of the highest amount of wastepaper incubated.

Keyword:     Cellulose cellulase newspaper sugars bioenergy A. niger.


Sibiya JBM, Wyk JPH. Bioconversion of waste newspaper into fermentable sugars at different temperatures with different Aspergillus niger cellulase concentrations. J App Biol Biotech. 2016; 4 (04): 069-074. DOI: 10.7324/JABB.2016.40408

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Klem D, Heublein B, Fink H, BahnA. Cellulose: Fascinating Biopolymer and Sustainable Raw Material. Angewandte Chemie International Edition. 2005; 44(22):3358-3393.

2. Peresin MS, Habibi Y, Zoppe IO, Pawlak JJ, Rojas OJ. Nanofiber composites of polyvinyl alcohol and cellulose nanocrystals: Manufacture and characterization. Biomacromolecules. 2010; 11(3): 674-681.

3. Angles MN, Dufresne A. Plasticized starch/tunicin whiskers nanocomposites, I, Structural analysis. Macromolecules. 2009; 33(22): 8344-8353.

4. Roman M, Winter WT. Effect of sulfate groups from sulfuric acid hydrolysis on the thermal behavior of bacterial cellulose. Biomacromolecules. 2004; 5(5):1671-1677.

5. Garcia De Rodriguez NL, Thielemans W, Dufresne A. Sisal cellulose whiskers reinforced polyvinyl acetate nanocomposites. Cellulose. 2006; 13(3): 261-270.

6. Uddin AJ, Araki J, Gotoh Y. Characterization of the poly (vinylalcohol) cellulose whiskers gel spun fibers. Composites A: Applied Science and Manufacturing. 2011; 42(7): 741-747.

7. Dong H, Strawhecker KE, Snyder JF, Orlicki TA, Reiner R S, Rudie A W. Cellulose nanocrystals as a reinforcing material forelectrospun poly (methyl methacrylate) fibers: formation, properties and nanomechanical characterization carbohydrate. Carbohydrate Polymers. 2012; 87 :2488-2495.

8. Zang Y, Yue D, Nie Y. Greenhouse gas emissions from two stage land filling of municipal solid waste. Atmospheric Environment. 2012; 55: 139-143.

9. Li Y, Zhao X, Li Y, Li X. Waste incineration industry and development policies in China. Waste Management. 2015; 46: 234-241.

10. Sun Y, Yang G, Jia Z, Wen C, Zhang L. ACTA hydrolysis of corn stover using hydrochloric acid: Kinetic modelling and statistical optimization. Chemical Industry and Chemical Engineering Quarterly. 2014; 20 (4): 531-539.

11. Girisuta B, Janssen LPBM, Heeres HJ. Kinetic study on the acid - catalyzed hydrolysis of cellulose to Levulinic acid. Industrial Engineering and Chemical Research.2007; 46 (6):1696 - 1708.

12. Bensah EC, Mewsah M. Chemical pretreatment methods for ethanol: Technologies and innovation. International Journal of Chemical Engineering. 2013; 20(13):21-40.

13. Patagundi BI, Shivasharan CT, Kaliwal BB. Isolation and characterization of cellulose producing bacteria from soil. International Journal of Current Microbiology and Applied Sciences. 2014; 3(50): 59 - 69.

14. Chouhan PK. Production of fungal cellulose from agriculture soil isolates using CMC as substrate under submerge fermentation technology. World Journal of Pharmacy and Pharmaceutical Sciences. 2014; 3(2): 1976 - 1985.

15. Gupta R, Lee YY. Mechanism of cellulase reaction on pure cellulosic substrates. Biotechnology and Bioengineering. 2008; 102(6): 1570 - 1581. DOI: 10. 1002/bit. 22195.

16. Juturu V, Wu JC. Microbial cellulases: Engineering, production and applications. Renewable and Sustainable Energy Reviews. 2014; 33:118-203.

17. Trudeau DL, Lee TM, Arnold FH. Engineered thermo stable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures. Biotechnology and Bioengineering. 2014; III(12): 2390 - 2397.

18. Ibrahim ASS, Diwany EL. Isolation and identification of new cellullases producing thermophilic bacteria from an Egyptian hot spring and some properties of crude enzyme. Australian Journal of Basic and Applied Sciences. 2007; 1(4): 473-478.

19. Miller GL. Use of dinitrosalicylic acid reagent for the determination of reducing sugars. Analytical Chemistry. 1959; 31(3):426-428.

20. Adsul M, Soni SK, Bhargona SK, Bawsai V. Facile approach for the dispersion of regenerated cellulose in aqurous system in the form of nanoparticles. Biomacromolecules. 2012; 13(9):2890-2895.

21. Gericke M, Fardim P, Heinre T. Ionic liquids -promising but challenging solvents for homogeneous derivatization of cellulose. Molecules.2012;17(6): 7458 - 7502.

22. Chander KR, Mehta R, Gupta R, Sharma KK. FED batch enzymatic saccharification of newspaper cellulosic improves the content in the hydrolyzates and eventually the ethanol fermentation by Saccharomyces cerevisiae. Biomass and Bioenergy. 2010; 34: 1189 - 1194.

23. Nedjma S, Djidjelli H, Boukerrow A, Benachour D, Chibani N. Deinked and acetylated fiber of newspaper. Journal of Applied Polymer Science. 2013; 127(2):4795-4801.

24. Lee D H, Cho EY, Kim CJ, Kim SB. Pretreatment of waste newspaper using ethylene glycol for bioethanol production. Biotechnology and Bioprocess Engineering. 2010;15(6):1094 - 1101.

25. JinC, Han S, Li J, Sun Q. Fabrication of cellulose - based aerogels from waste newspaper without any pretreatment and their use for absorbents. Carbohydrate Polymers. 2015;123:150-156.

26. Boshoff S, Gottumukkala LD, Van Rensburg E, Gorgens J. Paper sludge (PS) to bioethanol: Evaluation of virgin and recycle mill sludge for low enzyme, high-solids fermentation. Bioresource Technology. 2016; 203:103-111.

27. Sharada R, Venkateswarlu G, Venkateshwar S, Anand Rao M. Production of cellulose - A Review. International Journal of Pharmaceuticals, Chemical and Biological Sciences. 2013; 3(4):1070-1090.

28. Van Wyk JPH, Sibiya JBM. Saccharification of ink covered office paper by different concentrations of cellulase from Trichoderma viride. Journal of Chemical and Pharmaceutical Research. 2014; 6(10): 9-17.

Article Metrics
72 Views 51 Downloads 123 Total



Related Search

By author names

Similar Articles

Biocatalysis of agro-processing waste by marine Streptomyces fungicidicus strain RPBS-A4 for cellulase production

Rajanikanth Akurathi, Damodharam Thoti

Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

Punam Yadav , Suresh Korpole, Gandham S Prasad, Girish Sahni , Jyoti Maharjan, Lakshmaiah Sreerama, Tribikram Bhattarai

Sequestration and purification of essential enzyme from Pleurotus florida compost waste and its application studies

S Karthika Devi, N Prabhu , S Monika, N M Swapna, S Vaishali, T Gajendran

Production and purification of extracellular fungal cellulases using agricultural waste

Abishna Burugu, Dheerendra Kumar Suman, Chandrasekhar Chanda

Optimization and statistical modeling of microbial cellulase production using submerged culture

Pratibha Maravi, Anil Kumar

Bioprospecting for extracellular enzymes from endophytic bacteria isolated from Vigna radiata and Cajanus cajan

Namita Bhutani, Rajat Maheshwari, Pradeep Kumar, Rakhi Dahiya, Pooja Suneja

Isolation and identification of bacteria with cellulose-degrading potential from soil and optimization of cellulase production

Shweta Ashok Bhagat, Seema Sambhaji Kokitkar

Upsurge production of cellulase from maize stover under soildstate conditions mediated by Streptomyces enissocaesilis DQ026641

Ashok Sudarshan, Siddanna Renuka, Sirasagar Reshma, Bhalerao Shilanjali, Dayanand Agsar

Solid-state fermentation of groundnut (Arachis hypogaea) shell using Trichoderma sp., tape yeast, and tempeh yeast to produce cellulase

Muhammad Yusuf Abduh, Chalil Rizqullah Ramadhan, Alfanny Putri Fadhlilah, Siti Dhiffah Nabilah Abdul, Khairul Hadi Burhan

Isolation and Characterization of Cellulase-Producing Myxobacterial Strain from the Unique Niche of Mirgund Wetland from the North-Western Himalayas

Daljeet Singh Dhanjal, Simranjeet Singh, Vijay Kumar, Praveen C. Ramamurthy, Chirag Chopra, Atif Khurshid Wani, Reena Singh, Joginder Singh

Role of calcium in increasing tolerance of Hyacinth bean to salinity

Myrene R. D’souza, Devaraj V. R.

Differential metabolic responses associated with drought tolerance in Egyptian rice

Amira Hassanein, Eman Ibrahim, Rania Abou Ali, Hanan Hashem

Estimation of solids and biodegraded metabolites in the raw and processed slurry of the two-phase Nisargruna biogas plant

Shubhada Nayak, Sharad Kale

Recent advances in the processing of Napier grass (Pennisetum purpureum Schumach) as a potential bioenergy crop for bioethanol production

Priya Chamoli, Samiksha Jhildiyal, Palak Agrawal, Navin Kumar, Pallavi Singh