Research Article | Volume: 4, Issue: 4, July-August, 2016

Evaluation of Beauveria sp strains, conidial concentration and immersion times on mortality rate of bovine tick (Boophilus sp).

Oscar Miguel Domínguez-GaldámezMaría Ángela Oliva-Llaven Gabriela Aguilar-Tipacamú Paula MendozaNazar Benigno Ruiz-Sesma Gerardo Uriel Bautista-Trujillo José Miguel Culebro-Ricaldi Federico Antonio Gutiérrez-Miceli   

Open Access   

Published:  Aug 26, 2016

DOI: 10.7324/JABB.2016.40407

Ticks (Boophilus sp) are one of the main problems that affect the performance of bovine production in tropic and sub-tropical. Organophosphate acaricides are the usual tick control method, however the ecological damage and cross-resistance to those acaricides is leading to the search for alternatives. Beauveria bassiana can be used as a biological control, however, laboratory bioassays with ticks showed mortality of 20 to 50% only. The aim of this investigation was to study the effect of five strains of Beauveria sp, two conidial concentrations and three immersion times on mortality rate of ticks. Five strains of Beauveria sp isolated in México were used and cultivated in liquid medium until 107 and 108 conidia/mL were obtained. For in vitro bioassays, ticks were collected from bovine, immersed in conidial suspensions for 20, 30 and 40 seconds. Mortality rate was recorded daily up to 10 days.Strain type was the main factor and 23-D Beauveria strain had the highest value (100%) of mortality rate after ten days of incubation with ticks. The best immersion time was 20 s and the best conidial concentration was 108 conidial/ml. In conclusion, native Beauveria sp strains could be an alternative viable for ticks control in bovine, however field test and Beauveria identification are necessary.

Keyword:     Bovine ticksbiological controlin vitro bioassaysnative Beauveria sp strains.


Domínguez-Galdámez OM, Oliva-Llaven MA, Aguilar-Tipacamú G, Mendoza-Nazar P, Ruiz-Sesma B, Bautista-Trujillo GU, Culebro-Ricaldi M, Gutiérrez-Miceli FA. Evaluation of Beauveria sp strains, conidial concentration and immersion times on mortality rate of bovine tick (Boophilussp). J App Biol Biotech. 2016; 4 (04): 064-068. DOI: 10.7324/JABB.2016.40407

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Torgerson PR, Macpherson, CNL. The socioeconomic burden of parasitic zoonoses: Global trends. Veterinary Parasitology.2011; 182: 79-95. doi:

2. Perry AS, Perry I, Yamamoto I,Ishaaya R, Perry Y. Environment: Retrospects and Prospects. Springer Science & Business Media, 14/03/2013 - 261p.

3. Xia J, Zhang C-R, Zhang S, Li F-F, Feng M-G. Analysis of whitefly transcriptional responses to Beauveria bassiana infection reveals new

insights into insect-fungus interactions. PLoS ONE. 2013;8: e68185. doi:10.1371/journal.pone.0068185

4. Muchenje Dzama K, Chimonyo M, Raats JG, Strydom PE. Tick susceptibility and its effects on growth performance and carcass characteristics of Nguni, Bonsmara and Angus steers raised on natural pasture. Animal Cambridge Journals. 2008;2:288-298. DOI: 10.1017/S1751731107001036

5. Andrew YL, R DaveyRJ, Miller George JE. Resistance to coumaphos and diazinon in Boophilus microplus (Acari: Ixodidae) and evidence for the involvement of an oxidative detoxification mechanism. J. Med. Entomol. 2003;40: 482-490

6. Sujeetha JA, RP, Sahayaraj K. Role of entomopathogenic fungus in pest management. In K. Sahayaraj (Ed.), Basic and Applied Aspects of Biopesticides. New Delhi: Springer India. 2014; pp. 31-46.

7. Daniel C, Wyss E. Field applications of Beauveria bassiana to control the European cherry fruit fly Rhagoletiscerasi. J Appl Entomol. 2010; 134: 675-681.

8. Torrado-Leon E, Montoya-Lerma J, Valencia-Pizo E.Sublethal effects of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina: Hyphomycetes) on the whitefly Bemisiatabaci (Gennadius)(Hemiptera: Aleyrodidae) under laboratory conditions. Mycopathologia. 2006; 162: 411-419.

9. Wraight S, Carruthers R, Jaronski S, Bradley C, Garza C. Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomycesfumosoroseus for microbial control of the silverleaf whitefly, Bemisiaargentifolii. Biol Control. 2000; 17: 203-217

10. Xiao G, Ying S-H, Zheng P, Wang Z-L, Zhang S, Xie X-Q, Feng M-G. Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana. Scientific Reports. 2012;2,483.doi:10.1038/srep00483.

11. Campos RA, JT Boldo, IC Pimentel, V Dalfovo, WL Araújo, JL Azevedo, MH Vainstein,Barros NM. Endophytic and entomopathogenic strains of Beauveria sp to control the bovine tick Rhipicephalus (Boophilus) microplus Genetics and Molecular Research. 2010; 9: 1421-1430.

12. Cañedo V, Ames T. Manual de Laboratorio para el Manejo de Hongos Entomopatógenos. Lima, Perú; Centro Internacional de la Papa (CIP). 2004. Lima, Perú, 62 p.

13. Faria M, Wraight SP. Biological control of Bemisiatabaci with fungi. Crop Prot. 2001; 20: 767-778.

14. Toledo AV, de Remes Lenicov AMM, Lastra CCL.Histopathology caused by the entomopathogenic fungi, Beauveria bassiana and Metarhiziumanisopliae, in the adult planthopper, Peregrinusmaidis, a maize virus vector. J Insect Sci. 2010; 10: 35.

Article Metrics
151 Views 52 Downloads 203 Total



Related Search

By author names

Similar Articles