Research Article | Volume: 4, Issue: 3, May-June, 2016

Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh

Md. Jakirul Islam A. T. M. Mahbub-E-Elahi Tanvir Ahmed Md. Kamrul Hasan   

Open Access   

Published:  Jun 21, 2016

DOI: 10.7324/JABB.2016.40308

The objectives of this study were to isolate the associated Salmonella spp. from cloacal swabs of broiler and their antibiogram studies. A total of 80 cloacal swabs comprising of 50 samples of apparently healthy broiler and 30 samples of diarrheic broiler were collected from different poultry farms at Sylhet, Bangladesh during January to June 2013. The samples were subjected for isolation and identification of Salmonella spp. through a series of conventional bacteriological studies like study of morphology, staining properties, and biochemical characteristics. In results, 48% (n= 24/50) swab samples of healthy broiler and 66.7% (n= 20/30) diarrheic broiler were found to be associated with Salmonella spp. Among the 44 positive Salmonella isolates 47.73% (n=21) were Salmonella pullorum, 36.36% (n=16) isolates were Salmonella gallinarum and the rest 15.91% (n=7) isolates were Salmonella typhimurium. Sensitivity test was conducted against 10 commonly used antibiotics, of which Penicillin-G, Erythromycin, Ampicillin, and Bacitracin were found to be resistant, and Ceftriaxone, Gentamycin, and Choloramphenicol showed considerably better sensitivity as compared to others. It is concluded that Salmonella spp. are present in broiler, and the bacteria can be clinically controlled by using Ceftriaxone, Gentamycin, and Choloramphenicol.

Keyword:     IsolationIdentificationSalmonella spp.Anti-biogram studyBroiler farm.


Islam MJ, Mahbub-E-Elahi ATM, Ahmed T, Hasan MK. Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh. J App Biol Biotech. 2016; 4 (03): 046-051. DOI: 10.7324/JABB.2016.40308

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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