Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh

Md. Jakirul Islam; A. T. M. Mahbub-E-Elahi; Tanvir Ahmed; Md. Kamrul Hasan

doi:10.7324/JABB.2016.40308

Abstract

The objectives of this study were to isolate the associated Salmonella spp. from cloacal swabs of broiler and their antibiogram studies. A total of 80 cloacal swabs comprising of 50 samples of apparently healthy broiler and 30 samples of diarrheic broiler were collected from different poultry farms at Sylhet, Bangladesh during January to June 2013. The samples were subjected for isolation and identification of Salmonella spp. through a series of conventional bacteriological studies like study of morphology, staining properties, and biochemical characteristics. In results, 48% (n= 24/50) swab samples of healthy broiler and 66.7% (n= 20/30) diarrheic broiler were found to be associated with Salmonella spp. Among the 44 positive Salmonella isolates 47.73% (n=21) were Salmonella pullorum, 36.36% (n=16) isolates were Salmonella gallinarum and the rest 15.91% (n=7) isolates were Salmonella typhimurium. Sensitivity test was conducted against 10 commonly used antibiotics, of which Penicillin-G, Erythromycin, Ampicillin, and Bacitracin were found to be resistant, and Ceftriaxone, Gentamycin, and Choloramphenicol showed considerably better sensitivity as compared to others. It is concluded that Salmonella spp. are present in broiler, and the bacteria can be clinically controlled by using Ceftriaxone, Gentamycin, and Choloramphenicol.

Keyword: Isolation Identification Salmonella spp.Anti-biogram study Broiler farm.

Citation:

Islam MJ, Mahbub-E-Elahi ATM, Ahmed T, Hasan MK. Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh. J App Biol Biotech. 2016; 4 (03): 046-051. DOI: 10.7324/JABB.2016.40308

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Reference

1. Saleh M, Seedorf J, Hartung J. Total count of bacteria in the air of three different laying hen housing systems. Dtsch Tierarztl Wochenschr. 2003; 110:94-97.

2. Das PM, Rajib DMM, Noor M, Islam MR. Relationship analysis on the proportional incidence of poultry disease in greater Mymensingh of Bangladesh. In: Proceeding of the 4th International Poultry Show & Seminar organized by World’s Poultry Science Association, Bangladesh Branch. 2005; pp.35-39.

3. Mead GC. Prospects for competitive exclusion treatment to control Salmonella and other food home pathogen in poultry. Veterinary Journal. 2000; 159:111-123.

4. Begum F, Khan MSR, Chowdhury KA, Rahman MM, Amin MM. Studies on immune response of chickens to fowl typhoid vaccine. Bangladesh Journal Microbiology. 1993; 10:51-56.

5. Haider MG, Hossain MG, Hossain MS, Chowdhury EH, Das PM, Hossain MM. Isolation and characterization of Enterobacteria Associated with Health and Disease in Sonali Chickens. Banganladesh Journal of Veterinary Medicine. 2004; 2(1):15-21.

6. Talha AFSM, Hossain MM, Chowdhury EH, Bari ASM, Islam MR, Das PM. Poultry diseases occurring in Mymensingh district of Bangladesh. The Bangladesh Veterinarian. 2001; 18:20-23.

7. Opitz HM, El-Begearmi M, Flegg P, Beane D. Effectiveness of five feed additives in chicks infected with Salmonella enteritidis phage type 13A. Poultry Research. 1993; 2:147-153.

8. Rahman H, Kumar A, Murugkar HV, Bhattacharyya DK, Shome BR, Shome R. Some epidemiological features of Salmonella typhimurium isolates from north east India. Indian Journal of Animal Sciences. 2004; 74(6):609-611.

9. Kapil A. The challenge of antimicrobial resistance: need to contemplate. Indian Journal of Medical Research. 2004; 121:83-91.

10. Carter GR, John R Cole Jr. Diagnostic procedure in Veterinary bacteriology and Mycology. 1985; pp.551-552, 556.

11. Cheesbrough M. Medical laboratory manual for tropical countries. Vol. 2. Microbiology. 1985; pp.400-480.

12. Merchant IA, Packer RA. Veterinary bacteriology and virology, 7th edn. The Iowa University Press, Ames, Iowa, USA. 1967; 286-306.

13. Bauer AW, Kirby WM, Sherris JC. Antibiotic susceptibility testing by a standard single disc method. American Journal of Clinical Pathology. 1966; 45:493-496.

14. Jakaria ATM, Islam MA, Khatun MM. Prevalence, characteristics and antibiogram profiles of Escherichia coli isolated from apparently healthy chickens in Mymensingh, Bangladesh. Microbes Health. 2012; 1:27-29.

15. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 21th informational supplement. CLSI document M100-S21. Clinical and Laboratory standards Institute. Wayne, pa. 2011; pp.114-117.

16. Gast RK. Paratyphoid Infections. In Diseases of Poultry, 10th ed; Calnek BW, Barnes HJ, Beard CW, McDoughald LR, Saif YM, Eds. Iowa State University press: Ames, IA, USA. 1997; pp.97–121.

17. Ahmed AKM, Islam MT, Haider MG, Hossain MM. Sero prevalence and pathology of naturally infected Salmonellosis in poultry with isolation and identification of causal agents. Journal of the Bangladesh Agricultural University. 2008; 6(2):327-334.

18. Hossain MA, Aalbaek B, Christensen JP, Elisabeth H, Islam MA, Pankaj K. Observations on experimental infection of Salmonella gallinarum in Fayoumi and Hyline layer chickens. Bangladesh Journal of Progressive Agriculture. 2006; 14:85-89.

19. Amin MM. Isolation and identification of Salmonella from wild birds, rodents and insects. M.S. thesis, Department of Microbiology and Hygiene, Faculty of Veterinary Sciences, Bangladesh Agricultural University, Mymensingh. 1969; pp.1-48.

20. Buxton A, Fraser G. Animal Microbiology Vol. 1. Blackwell Scientific Publications, Oxford, London, Edinburg, Melbourne. 1977; pp.93-157.

21. Hossain KM. Characterisation of bacteria isolated from diarrheic calves. M.S Thesis, Department of Microbiology and Hygiene. Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. 2002; pp.1-72.

22. Habrun B, Mitak M. Monitoring for faecal Salmonella spp. in poultry. Zagreb, Croatia: Croatian Veterinary Institute, Poultry Centre. Hrvatska. 2003; pp.161-163.

23. Lee TJ, Kim KS, Kwon YK, Kang MS, Mo IP, Kim JH, Tak RB. Prevalent characteristics of fowl typhoid in Korea. Journal of Veterinary Clinical Pathology. 2003; 20:155-158.

24. Sujatha K, Dhanalakshmi K, Rao AS. Isolation and characterization of Salmonella gallinarum from chicken. Indian Veterinary Journal. 2003; 80:473-474.

25. Muktaruzzaman M, Haider MG, Ahmed AKM, Alam KJ, Rahman MM, Khatun MB, Rahman MH, Hossain MM. Validation and refinement of Salmonella pullorum (SP) colored antigen for diagnosis of Salmonella infections in the field. International Journal Poultry Science. 2010; 9:801-808.

26. Freeman BA. Burrows Textbook of Microbiology. 22th edn. In: W. B. Saunders Company, Philadelphia, London, Toronto, Mexico City, Rio de Janerio, Sydney, Tokyo. 1985; pp.464-475.

27. Kwon YK, Kim A, Kang MS, Her M, Jung BY, Lee KM, Jeong W, Kwon JH. Prevalence and characterization of Salmonella gallinarum in the chicken in Korea during 2000 to 2008. Poultry Science. 2010; 89:36-42.

28. Sultana K, Bushra MA, Nafisa I. Evaluation of antibiotic resistance in clinical isolates of Salmonella typhi from Islamabad. Pakistan Journal of Zoology. 1992; 27:185-187.

29. Ferdous TA, Kabir SML, Amin MM, Hossain KMM. Identification and Antimicrobial Susceptibility of Salmonella species isolated from washing and rinsed water of Broilers in pluck shops. International Journal of Animal and Veterinary Advances. 2013; 5(1):1-8.

30. Roy SR, Rahman MB, Hassan J, Nazir KHMNH. Isolation and identification of bacterial flora from internal organs of Broiler and their antibiogram studies. Microbes and Health. 2012; 1(2):72-75.

31. Khan MFR, Rahman MB, Khan MSR, Nazir KHMNH, Rahman M. Antibiogram and plasmid profile analysis of isolated Poultry Salmonella of Bangladesh. Pakistan journal of biological Science, 2005; 8(11):1614-1619.

32. Saifullah MK, Mamun MM, Rubayet RM, Nazir KHMNH, Zesmin K, Rahman MT. Molecular detection of Salmonella spp. isolated from apparently healthy pigeon in Mymensingh, Bangladesh and their antibiotic resistance pattern. Journal of Advanced Veterinary and Animal Research. 2016; 3(1):51-55.

33. Manie T, Khan S, Veith W, Bro¨Zel VS, Gouws PA. 1998. Antimicrobial resistance of bacteria isolated from slaughtered and retail chicken in South Africa. Letters in Applied Microbiology. 1998; 26:253-258.

34. Banani M, Pourbakash SA, Khaki P, Nikookhesal GH. Serotyping and drug sensitivity of Salmonella isolates from commercial chicken and domestic pigeons submitted to Razi institute. Pajouhesh-va-sadandegi-in-Animal-Science. 2003; 59:92-96.

35. Shah AH, Korejo NA. Antimicrobial resistance profile of Salmonella serovars isolated from chicken meat. Journal of Veterinary and Animal Science. 2012; 2:40-46.

36. Kabir SML. Avian colibacillosis and salmonellosis: A closer look at epidemiology, pathogenesis, diagnosis, control and public health concerns. International Journal of Environmental Research and Public Health. 2010; 7:89-114.

37. Jahan F, Kabir SML, Amin MM. Identification and antimicrobial resistance profiles of Salmonellae isolated from the broiler dressing plants associated with their environments. Advanced Research Journal of Microbiology. 2013; 1(1):1-9.

38. De-Jong A, Thomas V, Simjee S, Godinho K, Schiessl B, Klein U, Butty P, Valle M, Marion H, Shryock TR. Pan- European monitoring of susceptibility to human use antimicrobial agents in enteric bacteria isolated from healthy food producing animals. Journal of Antimicrobial Chemotherapy. 2012; 67:638-51.

39. Khan MFR, Rahman MB, Sarker SK. Seroprevalence of Mycoplasma gallisepticum infection in poultry in some selected farms of Mymensingh. Journal of The Bangladesh Society for Agricultural Science and Technology. 2005; 2:1-4.

Abstract

HTML Full Text

Reference

Article Metrics

Related Search

Citiaion Alert By Google Scholar

Similar Articles