Research Article | Volume: 4, Issue: 3, May-June, 2016
Establishment of in vitro regeneration from petiole explants and assessment of clonal fidelity by ISSR markers in Luffa acutangula L.Roxb
An improved in vitro organogenesis was achieved from petiole explants of Luffa acutangula L. Roxb, a medicinally and economically important cucurbitaceous member. Morphogenetic response of petiole explants excised from in vitro grown plants has been studied with different concentrations of auxins and cytokinins. Higher percent of callus induction was occurred on Murashige and Skoog (MS) medium supplemented with 2mg/L BAP and 0.2mg/L NAA from the cut ends of petiole explants after 4 weeks of culture. Multiple shoots (8.28±0.85 shoots per explant) were induced after 2-3 weeks from the green compact callus on MS medium with 30g/L sucrose, 2.0mg/L BAP along with 0.2mg/L IAA. The elongation of proliferated shoots was achieved on same medium supplemented additionally with 0.3 mg/L GA3. The elongated shoots (1cm) were rooted on MS medium supplemented with 0.5 mg/L IBA. Rooted plants were acclimatized in the greenhouse and subsequently established in soil with 85% survival rate. Clonal fidelity of in vitro raised plantlets was determined using ISSR marker technique. The results showed the similar banding pattern with different ISSR primers ranging from 250bp to 1000bp and indicated the absence of polymorphism in donar mother plant and as well in in vitro regenerated plants.
Velivela Y, Narra M, Ellendula R, Kota S, Abbagani S. Establishment of In vitro regeneration from petiole explants and assessment of clonal fidelity by ISSR markers in Luffa acutangula L.Roxb. J App Biol Biotech. 2016; 4 (03): 041-045. DOI: 10.7324/JABB.2016.40307
1. Halder T, Gadgil VN. Morphogenesis in some species of the family cucurbitaceae. Tissue Culture of Economic Important Plants. In: A.N. Rao (ed.), 1982; 98-103.
2. Badgujar SB, Patil MB. Estimation of the chemical constituents of L. acutangula Var. amara. (Roxb). Natural Product Radiance. 2008; 7(1): 79-81.
3. Au TK, Collins RA, Lam TL, Ng TB, Fong WP, Wan DC. The plant ribosome inactivating proteins luffin and saponin are potent inhibitors of HIV-1 integrase. FEBS letters. 2000; 471 (2-3): 169-172.
4. Naresh Singh Gill, Rashmi Arora, Shiv Ranjan Kumar. Evaluation of Antioxidant, Anti-inflammatory and Analgesic potential of the Luffa acutangula Roxb.var. amara. Research Journal of Phytochemistry. 2011; 5(4):202-208.
5. Jusal P, Quanico, Evangeline C, Amor, Grace G, Perez. Analgesic and Hypoglycemic activities of Bixa orellana, Kyllinga monocephala and Luffa acutangula. Philippine Journal of Science. 2008; 137(1): 69-76.
6. Jadhav VB, Thakare VN, Suralkar AA, Despane AD, Naik SR. Hepatoprotective activity of Luffa acutangula against CCI4 and rifampicin induced liver toxicity in rats: a biochemical and histopathological evaluation. Indian Journal of Experimental Biology. 2010; 48(8): 822-829.
7. Nahar E, Haque ME, Sikdar B. Comparison of the effects of growth regulators on in vitro regeneration of ridge gourd and sponge gourd through shoot. J.bio.Sci. 2010; 18:88-93.
8. Roly ZY, Mahmudul M, Haque ME, Islam MA, Sikdar B. In vitro multiple shoot regeneration from cotyledonary nodes, shoot tips and nodal segments of ridge gourd. J Discovery Biotech. 2013; 4(9):4-8.
9. Syed Moideen R, Lakshmi Prabha A. In Vitro Plant regeneration of Luffa acutangula Roxb. Var amara Lin.: An Important Medicinal Plant. International Journal of Science and Research. 2015; 4(5):117-122.
10. Umamaheswari C, Ambethkar A, Shantha MF, Selvaraj N. In vitro multiple shoot regeneration from cotyledon explants of Luffa acutangula (L.) Roxb. International Journal of Current Biotechnology. 2014; 2(7):7-13.
11. Partha Sarathi Saha, Biswajit Ghosh. Micropropagation and in Vitro Flowering of Luffa acutangula (L.) Roxb.- an Important Vegetable Crop. International Journal of Bio-resource and Stress Management. 2014; 5(1):012-021.
12. Arnau G, Lallemand J, Bourgoin M. Fast and reliable strawberry cultivar identification using inter simple sequence repeat (ISSR) amplification.Euphytica. 2003; 129(1):69-79.
13. Mallesham B, Srinivas K, Deepa R, Mahender A,Venugopal Rao K, Kranthi Kumar G, Sadanandam A. An Efficient in vitro Leaf-based Regeneration and Evaluation of Genetic Fidelity Using ISSR Markers in Woodfordia fruticosa (L.) Kurz. Journal of Herbs, Spices & Medicinal Plants. 2012; 18:178-190.
14. Ramakrishna D, Muralikrishna N, Raghu E, Srinivas K, Shasthree T. Efficient in vitro propagation system via multiple shoot induction and assessment of clonal fidelity of regenerants in Citrullus colocynthis (l.) Schard. Plant Cell Biotechnology and Molecular Biology. 2015; 16(3&4):108-118
15. Kranthi kumar G, Murthy N.E, Nataraj P, Srinivas K, Prasad Babu A, Teixeira da Silva JA, Raju VS, Sadanandam A. ISSR Markers Reveal Genetic Polymorphism in Two Morphological Variants of Hyptis suaveolens Invasive to India. Medicinal and Aromatic Plant Science and Biotechnology. 2011; 5 (2):166-168.
16. Murashige T, Skoog FA. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962; 15:473-497.
17. Doyle JJ, Doyle JL. A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem Bull. 1987; 19:11-15.
18. Taha A, Wagiran A, Ghazali H, Huyop F, Parvez GKA. In vitro regeneration of Garden balsam, Impatiens baslamina using cotyledons derived seedlings. Biotechnology. 2009; 8(1):44-52.
19. Nabi SA, Rashid MM, Al-Amin M., Rasul MG. Organogenesis in Teasle Gourd. (Momordica dioica Roxb.).Plant Tiss Cult. 2002; 12(2):173-180.
20. Malik S, Zia M, Rehman R, Chaudhary F. In vitro plant regeneration from direct and indirect organogenesis of Momordica charantia L. Pak J Biol Sci. 2007; 10:4118-4122.
21. Manye Y, Maojun Z, Yu Z, Liqong L, Fang C. Establishment of in vitro regeneration system of Bitter Melon (Momordica charantia L.). High Technol Lett. 2004; 10:44-48.
22. Pierik RLM. In vitro propagation of higher plants. 1987; Martinus Nizhoof Publisher, Boston.
23. Selvaraj N, Vasudevan A, Manickavasagam M, Ganapathi A. In vitro organogenesis and plant formation in cucumber. Biol Plant. 2006b; 50:123-126.
24. Ananthakrishnan G, Xia X, Elman C, Singer S, Paris HS, Gal-On A, Gaba V. Shoot production in squash (Cucurbita pepo) by in vitro organogenesis. Plant Cell Rep. 2003; 21:739-746.
25. Islam R, Sarkar PK, Naderuzzaman ATM, Joarder OI. In vitro regeneration of plants from cotyledons of Momordica charantia L. Plant Tissue Cult. 1994; 4:105-109.
26. Giri CC, Shyamkumar B, Anjaneyulu C. Progress in tissue culture genetic transformation and application of biotechnology to tress: an overview.Trees.2004; 18:115-135.
27. Larkin PJ, Scowcroft WR. Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor. Appl.Genet.1981; 60:197-214.
28. Kumar N, Modi AR, Singh AS, Gajera BB, Patel AR, Patel MP, Subhash N. Assessment of genetic fidelity of micropropagated date palm (phoenix dactylifera L.) plants by RAPD & ISSR markers assay. Physiol Mol Biol Plants. 2010a; 16:207-213.
29. Raimondi JP, Camadro EL, Masuelli RW. Assessment of somaclonal variation in Asparagus by RAPD fingerprinting and cytogenetic analyses. Sci Hortic. 2001; 90:19-29.
30. Latto SK, Bamotra S, Saprudhar R, Khan S, Dhar AK. Rapid plant regeneration and analysis of genetic fidelity of in vitro derived plants of Chlorophytum arundinaceum Baker-an endangered medicinal herb. Plant Cell Rep. 2006; 25:499-506.
31. Yashodhara V, Muralikrishna N, Raghu E, Bharathkumar K, Rathnaprabha D, Sadanandam A. in vitro plant regeneration from petiole explants and assessment of genetic fidelity using ISSR markers in momordica charantia L. Plant Cell Biotechnology and Molecular Biology. 2016; 17(1&2):49-56.
Year
Month