Research Article | Volume: 4, Issue: 2, March-April, 2016

In vitro Propagation of Rhododendron griffithianum Wt.: An Endangered Rhododendron Species of Sikkim Himalaya

Kaushal K. Singh Mithilesh Singh and Archana Chettri   

Open Access   

Published:  Apr 21, 2016

DOI: 10.7324/JABB.2016.40211
Abstract

A reproducible protocol for in vitro propagation of R. griffithianum has been established. Multiple shoot proliferation from shoot-tip explants was occurred in the presence of 2-isopentenyl adenine (2-iP) alone or in combination with indole 3-butyric acid (IBA) in Anderson's medium. The best treatment for shoot regeneration was Anderson's medium supplemented with 5 µM 2-iP and 1.0 µM IBA, which promoted shoot proliferation in more than 75% culture with an average of 11.00 shoots per explants after twelve weeks. These shoots were successfully rooted on Anderson's liquid medium supplemented with 1.5 µM IBA. The rooted plantlets were hardened in greenhouse with 84% survival rate.


Keyword:     Multiple shootpropagationRhododendron griffithianumSikkim Himalaya.


Citation:

Singh KK, Singh M, Chettri A. In Vitro Propagation of Rhododendron griffithianum Wt.: An Endangered Rhododendron Species of Sikkim Himalaya. J App Biol Biotech. 2016; 4 (02): 072-075. DOI: 10.7324/JABB.2016.40211

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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