Decolorization of selected industrial synthetic dyes using laccase from an indigenous isolate strain SK1

Maegala Nallapan Maniyam Primeela Gunalan Hazeeq Hazman Azman Hasdianty Abdullah Nor Suhaila Yaacob   

Open Access   

Published:  Nov 07, 2024

DOI: 10.7324/JABB.2025.202058
Abstract

Textile industrial wastewater is among the most polluting wastewater globally, necessitating efficient and sustainable treatment methods. Therefore, this study investigates the use of extracellular laccase, which is eco-friendly and cost-effective, particularly from an indigenous isolate coded as strain SK1, for bioremediation. The laccase production was optimized, leading to a 68% yield improvement when temperature, pH, agitation, and inoculum size were set at 35°C, pH 8, 150 rpm, and 5% v/v, respectively, using banana peel as the substrate. This optimization also reduced the incubation period by 67%. Remarkably, more than 65% decolourization was achieved with 100 mg/l concentrations of various dyes, including Congo Red, Alizarin Yellow, Methyl Orange, Methyl Red, Methylene Blue, Crystal Violet, and Malachite Green, within 60 minutes of incubation without mediators. Furthermore, the laccase application removed 99% of the dye from real batik wastewater, rendering the treated water safe for irrigation, as evidenced by a 98% germination rate of Vigna radiata. These findings underscore the effectiveness and practicality of laccase for textile wastewater treatment. Future research should focus on immobilizing laccase to enhance its biocatalytic performance, ensuring a robust and scalable bioremediation approach for industrial applications.


Keyword:     Batik wastewater dye decolorization indigenous microorganism laccase activity optimization


Citation:

Maniyam MN, Gunalan P, Azman HH, Abdullah H, Yaacob NS. Decolorization of selected industrial synthetic dyes using laccase from an indigenous isolate strain SK1. J Appl Biol Biotech. 2024. Online First. http://doi.org/10.7324/JABB.2025.202058

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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