Decolorization of selected industrial synthetic dyes using laccase from an indigenous isolate strain SK1
Textile industrial wastewater is among the most polluting wastewater globally, necessitating efficient and sustainable treatment methods. Therefore, this study investigates the use of extracellular laccase, which is eco-friendly and cost-effective, particularly from an indigenous isolate coded as strain SK1, for bioremediation. The laccase production was optimized, leading to a 68% yield improvement when temperature, pH, agitation, and inoculum size were set at 35°C, pH 8, 150 rpm, and 5% v/v, respectively, using banana peel as the substrate. This optimization also reduced the incubation period by 67%. Remarkably, more than 65% decolourization was achieved with 100 mg/l concentrations of various dyes, including Congo Red, Alizarin Yellow, Methyl Orange, Methyl Red, Methylene Blue, Crystal Violet, and Malachite Green, within 60 minutes of incubation without mediators. Furthermore, the laccase application removed 99% of the dye from real batik wastewater, rendering the treated water safe for irrigation, as evidenced by a 98% germination rate of Vigna radiata. These findings underscore the effectiveness and practicality of laccase for textile wastewater treatment. Future research should focus on immobilizing laccase to enhance its biocatalytic performance, ensuring a robust and scalable bioremediation approach for industrial applications.
Maniyam MN, Gunalan P, Azman HH, Abdullah H, Yaacob NS. Decolorization of selected industrial synthetic dyes using laccase from an indigenous isolate strain SK1. J Appl Biol Biotech. 2024. Online First. http://doi.org/10.7324/JABB.2025.202058
1. Syed Shaharuddin SI, Shamsuddin MS, Drahman MH, Hasan Z, Mohd Asri NA, Nordin AA, et al. A review on the Malaysian and Indonesian batik production, challenges, and innovations in the 21st century. SAGE Open 2021;11(3):21582440211040128. https://doi.org/10.1177/21582440211040128 | |
2. Martuti NKT, Hidayah I, Margunani M, Alafima RB. Organic material for clean production in the batik industry: a case study of natural batik Semarang, Indonesia. Recycling 2020;5(4):28. https://doi.org/10.3390/recycling5040028 | |
3. Zakaria N, Rohani R, Wan Mohtar WHM, Purwadi R, Sumampouw GA, Indarto A. Batik effluent treatment and decolorization-a review. Water 2023;15(7):1339. https://doi.org/10.3390/w15071339 | |
4. Wei F, Shahid MJ, Alnusairi GS, Afzal M, Khan A, El-Esawi MA, et al. Implementation of floating treatment wetlands for textile wastewater management: a review. Sustainability 2020;12(14):5801. https://doi.org/10.3390/su12145801 | |
5. Kordbacheh F, Heidari G. Water pollutants and approaches for their removal. Mater Chem Horizons 2023;2(2):139-53. | |
6. Singh A, Pal DB, Mohammad A, Alhazmi A, Haque S, Yoon T, et al. Biological remediation technologies for dyes and heavy metals in wastewater treatment: new insight. Bioresour Technol 2022;343:126154. https://doi.org/10.1016/j.biortech.2021.126154 | |
7. Zakaria Z, Othman MR, Hasan SZ, Ahmad WW. Electrochemical degradation of reactive orange 16 by using charcoal-based metallic composite electrodes. Sains Malays 2019;48(4):791-801. https://doi.org/10.17576/jsm-2019-4804-11 | |
8. Maniyam M, Azman H, Abdullah H, Yaacob NS. Process optimization for efficacious biodecolorization of crystal violet by Malaysian Rhodococcus pyridinivorans using monothetic analysis. J Appl Biol Biotechnol 2022;10(2):107-13. https://doi.org/10.7324/JABB.2022.10s212 | |
9. Wang J, Chang F, Tang X, Li W, Yin Q, Yang Y, et al. Bacterial laccase of Anoxybacillus ayderensis SK3-4 from hot springs showing potential for industrial dye decolorization. Ann Microbiol 2020;70:1- 9. https://doi.org/10.1186/s13213-020-01593-6 | |
10. Sharma N, Leung IK. Characterisation and optimisation of a novel laccase from Sulfitobacter indolifex for the decolourisation of organic dyes. Int J Biol Macromol 2021;190:574-84. https://doi.org/10.1016/j.ijbiomac.2021.09.003 | |
11. Maniyam MN, Azman HH, Abdullah H, Yaacob NS. Response surface methodology as an optimization tool to achieve an effective decolourization of crystal violet by the Malaysian Rhodococcus pyridinivorans strain. Biomass Convers Biorefin 2024;14(10):11023- 34. https://doi.org/10.1007/s13399-022-03098-5 | |
12. ?a?maz S, Gedikli S, Aytar P, Güngörmedi G, Çabuk A, Hür E, et al. Decolorization potential of some reactive dyes with crude laccase and laccase-mediated system. Appl Biochem Biotechnol 2011;163:346- 61. https://doi.org/10.1007/s12010-010-9043-8 | |
13. Javadzadeh SG, Asoodeh A. A novel textile dye degrading extracellular laccase from symbiotic bacterium of Bacillus sp. CF96 isolated from gut termite (Anacanthotermes). Int J Biol Macromol 2020;145:355-63. https://doi.org/10.1016/j.ijbiomac.2019.12.205 | |
14. Wi?niewska KM, Twarda-Clapa A, Bia?kowska AM. Screening of novel laccase producers-isolation and characterization of cold-adapted laccase from Kabatiella bupleuri G3 capable of synthetic dye decolorization. Biomolecules 2021;11(6):828. https://doi.org/10.3390/biom11060828 | |
15. Gogotya A, Nnolim NE, Digban TO, Okoh AI, Nwodo UU. Characterization of a thermostable and solvent-tolerant laccase produced by Streptomyces sp. LAO. Biotechnol Lett 2021;43:1429- 42. https://doi.org/10.1007/s10529-021-03131-z | |
16. Mehandia S, Sharma SC, Arya SK. Isolation and characterization of an alkali and thermostable laccase from a novel Alcaligenes faecalis and its application in decolorization of synthetic dyes. Biotechnol Rep 2020;25:e00413. https://doi.org/10.1016/j.btre.2019.e00413 | |
17. Ali NS, Huang F, Qin W, Yang TC. Identification and characterization of a new Serratia proteamaculans strain that naturally produces significant amount of extracellular laccase. Front Microbiol 2022;13:878360. https://doi.org/10.3389/fmicb.2022.878360 | |
18. Abdelgalil SA, Soliman NA, Abo-Zaid GA, Abdel-Fattah YR. Bioprocessing strategies for cost-effective large-scale production of bacterial laccase from Lysinibacillus macroides LSO using bio-waste. Int J Environ Sci Technol 2021;19:1633-52. https://doi.org/10.1007/s13762-021-03231-3 | |
19. Maia LF, Hoyos SLM, Watanabe AK, Moralez ATP, Furlaneto MC, de Souza Daniel JF. Extra and intracellular laccase titers of xylophagic bacteria isolated from adult termite. Acta Sci Technol 2021;43:e51805. https://doi.org/10.4025/actascitechnol.v43i1.51805 | |
20. Mohd Israfi NA, Mohd Ali MIA, Manickam S, Sun X, Goh BH, Tang SY, et al. Essential oils and plant extracts for tropical fruits protection: from farm to table. Front Plant Sci 2022;13:999270. https://doi.org/10.3389/fpls.2022.999270 | |
21. Ibrahim UK, Kamarrudin N, Suzihaque MUH, Abd Hashib S. Local fruit wastes as a potential source of natural antioxidant: an overview. IOP Conf Ser Mater Sci Eng 2017;206(1):012040. https://doi.org/10.1088/1757-899X/206/1/012040 | |
22. Cen Q, Wu X, Cao L, Lu Y, Lu X, Chen J, et al. Green production of a yellow laccase by Coriolopsis gallica for phenolic pollutants removal. AMB Express 2022;12(1):96. https://doi.org/10.1186/s13568-022-01434-6 | |
23. Zehra M, Syed MN, Sohai M. Banana peels: a promising substrate for the coproduction of pectinase and xylanase from Aspergillus fumigatus MS16. Pol J Microbiol 2020;69(1):19-26. https://doi.org/10.33073/pjm-2020-002 | |
24. Vipotnik Z, Michelin M, Tavares T. Biodegradation of chrysene and benzo [a] pyrene and removal of metals from naturally contaminated soil by isolated Trametes versicolor strain and laccase produced thereof. Environ Technol Innov 2022;28:102737. https://doi.org/10.1016/j.eti.2022.102737 | |
25. Edoamodu CE, Nwodo UU. Enterobacter sp. AI1 produced a thermo-acidic-tolerant laccase with a high potential for textile dyes degradation. Biocatal Agric Biotechnol 2021;38:102206. https://doi.org/10.1016/j.bcab.2021.102206 | |
26. Kumar A, Singh AK, Ahmad S, Chandra R. Optimization of laccase production by Bacillus sp. strain AKRC01 in presence of agro-waste as effective substrate using response surface methodology. J Pure Appl Microbiol 2020;14(1):351-62. https://doi.org/10.22207/JPAM.14.1.36 | |
27. Omeje KO, Nnolim NE, Ezema BO, Ozioko JN, Eze SO. Synthetic dyes decolorization potential of agroindustrial waste-derived thermo-active laccase from Aspergillus species. Biocatal Agric Biotechnol 2020;29:101800. https://doi.org/10.1016/j.bcab.2020.101800 | |
28. Thakkar AT, Bhatt SA. Isolation, identification and optimization of laccase from Alternaria alternata. J Appl Biol Biotechnol 2020;8(3):64-9. https://doi.org/10.7324/JABB.2020.803012 | |
29. Navas LE, Carballo R, Levin L, Berretta MF. Fast decolorization of azo dyes in alkaline solutions by a thermostable metal-tolerant bacterial laccase and proposed degradation pathways. Extremophiles 2020;24:705-19. https://doi.org/10.1007/s00792-020-01186-w | |
30. Coria-Oriundo LL, Battaglini F, Wirth SA. Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system. Ecotoxicol Environ Saf 2021;217:112237. https://doi.org/10.1016/j.ecoenv.2021.112237 | |
31. Buzzo BB, Lima NSM, Pereira PAM, Gomes-Pepe ES, Sartini CCF, Lemos EGDM. Lignin degradation by a novel thermophilic and alkaline yellow laccase from Chitinophaga sp. Microbiol Spectr 2024;12(6):e04013-23. https://doi.org/10.1128/spectrum.04013-23 | |
32. Sondhi S, Kaur R, Madan J. Purification and characterization of a novel white highly thermo stable laccase from a novel Bacillus sp. MSK-01 having potential to be used as anticancer agent. Int J Biol Macromol 2021;170:232-8. https://doi.org/10.1016/j.ijbiomac.2020.12.082 | |
33. Rampinelli JR, Melo MPD, Arbigaus A, Silveira MLD, Wagner TM, Gern RM, et al. Production of Pleurotus sajor-caju crude enzyme broth and its applicability for the removal of bisphenol A. An Acad Bras Ciênci 2021;93(1):e20191153. https://doi.org/10.1590/0001-3765202120191153 | |
34. Umar A, Ahmed S. Optimization, purification and characterization of laccase from Ganoderma leucocontextum along with its phylogenetic relationship. Sci Rep 2022;12(1):2416. https://doi.org/10.1038/s41598-022-06111-z | |
35. Albulaihed Y, Adnan M, Jamal A, Snoussi M, Patel K, Patel M. Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes. Bioresour Bioprocess 2023;10(1):80. https://doi.org/10.1186/s40643-023-00703-x | |
36. Pinheiro VE, Michelin M, Vici AC, de Almeida PZ, Teixeira de Moraes Polizeli MDL. Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray. Bioprocess Biosyst Eng 2020;43:507-14. https://doi.org/10.1007/s00449-019-02245-z | |
37. Geethanjali PA, Gowtham HG, Jayashankar M. Optimization of culture conditions for hyper-production of laccase from an indigenous litter dwelling fungus Mucor circinelloides GL1. Environ Sustain 2020;3:481-95. https://doi.org/10.1007/s42398-020-00137-7 | |
38. Umar A, Abid I, Elshikh MS, Dufossé L, Abdel-Azeem AM, Ali I. Agitation role (dissolved oxygen) in production of laccase from newly identified Ganoderma multistipitatum sp. nov. and its effect on mycelium morphology. BMC Microbiol 2023;23:280. https://doi.org/10.1186/s12866-023-03009-2 | |
39. Hamill PG, Stevenson A, McMullan PE, Williams JP, Lewis AD, Stevenson KE. Microbial lag phase can be indicative of, or independent from, cellular stress. Sci Rep 2020;10(1):5948. https://doi.org/10.1038/s41598-020-62552-4 | |
40. Rajeswari M, Bhuvaneswari V. Production of extracellular laccase from the newly isolated Bacillus sp. PK4. Afr J Biotechnol 2016;15(34):1813-26. https://doi.org/10.5897/AJB2016.15509 | |
41. Sharma A, Muthupriya M, Raj R, Shameen Z, Veena SM, Niyonzima FN, et al. Properties of laccase of Bacillus marisflavi strain BB4 and its synthetic dyes decolorization analysis. Proc Natl Acad Sci India Sect B Biol Sci 2021;91(2):477-85. https://doi.org/10.1007/s40011-021-01235-0 | |
42. Hasan S, Anwar Z, Khalid W, Afzal F, Zafar M, Ali U, et al. Laccase production from local biomass using solid state fermentation. Fermentation 2023;9(2):179. https://doi.org/10.3390/fermentation9020179 | |
43. Varjani S, Rakholiya P, Ng HY, You S, Teixeira JA. Microbial degradation of dyes: an overview. Bioresour Technol 2020;314:123728. https://doi.org/10.1016/j.biortech.2020.123728 | |
44. Paraschiv G, Ferdes M, Ionescu M, Moiceanu G, Zabava BS, Dinca MN. Laccases-versatile enzymes used to reduce environmental pollution. Energies 2022;15(5):1835. https://doi.org/10.3390/en15051835 | |
45. Asadi E, Makhdoumi A, Asoodeh A. Laccase mediator system obtained from a marine spore exhibits decolorization potential in harsh environmental conditions. Ecotoxicol Environ Saf 2020;191:110184. https://doi.org/10.1016/j.ecoenv.2020.110184 | |
46. Sundarajoo A, Maniyam MN, Azman HH, Abdullah H, Yaacob NS. Rhodococcus strain UCC 0010 as green biocatalyst for enhanced biodecolourization of Congo red through response surface methodology. Int J Environ Sci Technol 2022;19:3305-22. https://doi.org/10.1007/s13762-021-03400-4 | |
47. Motamedi E, Kavousi K, Motahar SFS, Ghaffari MR, Mamaghani ASA, Salekdeh GH, et al. Efficient removal of various textile dyes from wastewater by novel thermo-halotolerant laccase. Bioresour Technol 2021;337:125468. https://doi.org/10.1016/j.biortech.2021.125468 | |
48. El-Bendary MA, Ezzat SM, Ewais EA, Al-Zalama MA. Optimization of spore laccase production by Bacillus amyloliquefaciens isolated from wastewater and its potential in green biodecolorization of synthetic textile dyes. Prep Biochem Biotechnol 2021;51(1):16-27. https://doi.org/10.1080/10826068.2020.1786698 | |
49. Toker SK, Evlat H, Koçyi??i?t A. Screening of newly isolated marine-derived fungi for their laccase production and decolorization of different dye types. Reg Stud Mar Sci 2021;45:101837. https://doi.org/10.1016/j.rsma.2021.101837 | |
50. Liu J, Chen J, Zuo K, Li H, Peng F, Ran Q, et al. Chemically induced oxidative stress improved bacterial laccase-mediated degradation and detoxification of the synthetic dyes. Ecotoxicol Environ Saf 2021;226:112823. https://doi.org/10.1016/j.ecoenv.2021.112823 | |
51. Unuofin JO. Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: from dye decolourization to denim bioscouring. Biotechnol Rep 2020;25:e00409. https://doi.org/10.1016/j.btre.2019.e00409 | |
52. Jiang J, Deng JL, Wang ZG, Chen XY, Wang SJ, Wang YC. Characterization of a new laccase from Vibrio sp. with pH-stability, salt-tolerance and decolorization ability. Molecules 2023;28(7):3037. https://doi.org/10.3390/molecules28073037 | |
53. Jain D, Navariya JK, Bhojiya AA, Singh A, Mohanty SR, Upadhyay SK. Bioprospecting of novel ligninolytic bacteria for effective bioremediation of agricultural by-product and synthetic pollutant dyes. Microbiol Res 2023;270:127330. https://doi.org/10.1016/j.micres.2023.127330 | |
54. Iqbal K, Nadeem A, Zafar U. Biostoning of textile effluent with laccase enzyme. Bangladesh J Sci Ind Res 2021;56(2):115-24. https://doi.org/10.3329/bjsir.v56i2.54318 | |
55. Wang X, Jiang J, Gao W. Reviewing textile wastewater produced by industries: characteristics, environmental impacts and treatment strategies. Water Sci Technol 2022;85(7):2076-96. https://doi.org/10.2166/wst.2022.088 | |
56. Uddin F. Environmental hazard in textile dyeing wastewater from local textile industry. Cellulose 2021;28(17):10715-39. https://doi.org/10.1007/s10570-021-04228-4 | |
57. Selim MT, Salem SS, Mohamed AA, El-Gamal MS, Awad MF, Fouda A. Biological treatment of real textile effluent using Aspergillus flavus and Fusarium oxysporium and their consortium along with the evaluation of their phytotoxicity. J Fungi 2021;7(3):193. https://doi.org/10.3390/jof7030193 | |
58. Bilal Y, Sarwar T, Mazhar E, Mahmood F, Shahzad T, Al-Farraj DA, et al. Reduction in phytotoxicity of a textile wastewater against Vigna radiata using Citrobacter sp. M41 in a bioaugmented packed bed column bioreactor. J King Saud Univ Sci 2024;36(2):103030. https://doi.org/10.1016/j.jksus.2023.103030 | |
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