Research Article | Volume 12, Issue 6, November, 2024

Application of soil amendments: investigation of incubation time on some soil solution properties of Kien Giang, Vietnam

Nguyen Van Phuong   

Open Access   

Published:  Sep 16, 2024

DOI: 10.7324/JABB.2024.188850
Abstract

Soil amendments can affect soil properties such as pH, EC, available phosphorus (P), and ammonium nitrogen (NH4+). The aim of this study was to determine the potential influence of submergence incubation time on some soil properties using soil amendments under controlled laboratory conditions suitable to the characteristics of rice-growing areas in Kien Giang, Vietnam. The experiment included treating soil supplemented with lime (at ratios of 0.0, 0.4, 0.6, and 1.0%) and soil supplemented with lime (0.4%) combined with alkalized biochar (at ratios 0.0, 0.2, 0.4, and 0.8%). The treatments were incubated in the laboratory under submerged conditions, and pH, EC, available phosphorus (P), and ammonium nitrogen (NH4+) parameters were measured at 7, 14, 21, 35, and 60 days. The results showed that soil supplemented with lime improved pH and EC, and maintained high phosphorus release for 21 days, but did not improve NH4+ release. In contrast, soil supplemented with lime (0.4%) combined with alkalized biochar improved pH and EC, maintained high levels of phosphorus and NH4+ content up to 35 days. The results showed that using lime combined with alkalized biochar to improve soil in rice-growing areas has many advantages compared to using lime alone. Therefore, understanding the contribution of soil incubation time with amendments to soil properties is an important prerequisite for developing a strategy to ensure crop productivity.


Keyword:     EC Phosphorus NH4+ pH Rice cultivation Incubation time


Citation:

Phuong NV. Application of soil amendments: investigation of incubation time on some soil solution properties of Kien Giang, Vietnam. J App Biol Biotech. 2024;12(6):88-95. http://doi.org/10.7324/JABB.2024.188850

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. INTRODUCTION

According to the current trend of intensive agriculture, the possibility of nutrient depletion or imbalance has occurred regularly in cropping systems. Furthermore, the recent energy crisis has increased the price of inorganic fertilizers, which has contributed to pressure on soil nutrients and crop yields [1,2]. Modern agricultural practices emphasize integrated nutrient management practices that maintain soil fertility over longer periods and more stable crop yields. The application of organic and inorganic fertilizers alone gives lower performance than integrated nutrient management practices [2]. Especially when integrated with organic amendments, it has significantly improved soil quality and ecosystem sustainability as a buffering agent, source of plant nutrients, and enhanced soil microbial biomass [2].

Soil pH reflects the overall chemical state of the soil and affects the entire range of chemical and biological processes occurring in the soil [2]. Electrical conductivity (EC) of soil serves as an indirect measure of both total dissolved solids and the mineralization/decomposition of organic matter [3]. Phosphorus is an essential element for plant growth. Phosphorus (P) deficiency often occurs in rice fields due to low solubility and mobility in soil and high immobilization capacity [4,5]. Ammonium nitrogen, NH4+, is the main source of N nutrients for soil. NH4+ in each type of soil changes (fixed or released) differently depending on the type of amendment used, mineral composition, pH, and organic content [6].

The Veitnam Mekong Delta, including Kien Giang Province, is a high-yield rice-growing area, contributing significantly to Vietnam’s rice output [7]. To maintain stable productivity, in addition to using inorganic fertilizers, it is necessary to combine with amendments (inorganic and organic) to maintain soil quality parameters in rice-growing areas in Giang Thanh District, Kien Province. Various studies have shown that the use of amendments has a significant effect on soil physicochemical indices such as pH, EC, available phosphorus, and ammonium nitrogen. These changes may be due to an increase in organic matter associated with proton consumption during neutralization, decarboxylation, decomposition, and adsorption of organic molecules [3]. One of the characteristics of the Mekong Delta soil is activated alum soil (acid sulfate soil–Orthi Thionic Fluvisols WRB), which has strong acidity, so the use of pH-improving substances is very frequent.

Using lime increases soil pH, soil available P content, cation exchange capacity (CEC) [8], and base saturation, and reduces mobile Al concentration [9]. However, using lime for sustainable agriculture is not economically and environmentally feasible due to costs and unrecoverable resource exploitation. Biochar produced from the low-temperature pyrolysis of organic material is a carbon-rich substance that has several important features that improve pH and alter the availability of nutrients such as phosphorus (e.g., available phosphorus is significantly increased by using biochar, whereas previously it was fixed by high levels of Al and Fe in the soil) [9]. Besides, biochar can regulate soil N dynamics by promoting nitrification, reducing denitrification, adsorbing NH4+, and reducing ammonia volatilization [10]. However, the use of biochar has also been reported to impair nutrient availability, with limited improvement in acidity, especially when soils have very low pH [11]. An alternative method to reduce biochar application rates could be to use a liquid biochar extract that can be sprayed, or irrigated [12]. Therefore, the use of alkalized biochar has been proposed. Dak Lak, Vietnam is the country’s leading coffee-growing province with an area of more than 200,000 hectares, an annual output of about 550,000 tons of finished products [13], and about 300,000 tons of waste coffee husks, which is a rich source of raw materials for biochar production.

To improve understanding of crop nutrient requirements, knowledge of soil characteristics after the application of amendments is needed. Therefore, it is necessary to monitor changes in soil properties over time. The objective of the current study is to determine the potential effects of incubated time under submerged conditions when using integrated lime and alkaline biochar to improve some physicochemical properties of the soil sample.


2. MATERIALS AND METHODS

2.1. Sample Collection

The soil sample was taken from Vinh Phu, Giang Thanh District, Kien Giang, Vietnam, located at 10°24′34.7′′N 104°45′34.0′′E. The sampling area selected was the largest rice-growing area in the region, with stable productivity. Five sub-samples were collected from a 10-m diameter sampling area at a depth of 0–10 cm. These sub-samples included four from each corner and one from the central diagonal position. The sub-samples were then thoroughly mixed to create a composite sample for further analysis. Soil samples should be stored in plastic bags or PE bottles at a temperature of 4°C, with the labels stating the full location, sample collection date of July 28, 2022, and sample collection time of 10:07 a.m. Soil samples were transported to the laboratory, air-dried, ground, and sieved through a 2-mm sieve. Later, the samples were analyzed to determine particle density, bulk density, total carbon (TOC), pH, EC, soil structure, ammonium, and available P in soil. Coffee husks were obtained in January 2019 from a farm located at 12°34′43.8″N, 108°01′39.3″E in Pong Drang, Krong Buk Town, Dak Lak Province, Vietnam.

2.2. Analytical Method

Bulk density, particle density, porosity, and CEC [8] of soil were determined [14]; soil texture was determined by the hydrometer method [14]; soil pH (with H.O ratio 1:5) was determined by Hanna–HI 8314 pH meter; TOC of soil was determined by Walkley–Black method [14]; available phosphorus was determined according to Olsen method; and NH4+ was extracted according to ISO 14225:1998 [14].

A total of 1 g of air-dried soil was weighed and digested in a muffle furnace at 550°C for 1 h to convert organically bound P to inorganic forms. The digest was then extracted with 25 mL of 1 N HCl using a reflux condenser for 1 h to solubilize inorganic P. After cooling, the extracts were diluted to volume in 100 mL volumetric flasks, and the total P concentration was determined colorimetrically [15].

2.3. Experimental Setup

2.3.1. Tools: chemicals and equipment

Laboratory apparatus must be cleaned before use by filling it with 1 M nitric acid for at least 24 h and then rinsing it with demineralized water. Distilled water was used, which was filtered by an ultra-clean water purifier Model: EASYpure II RF from Thermo Scientific, USA. The chemicals used in the experiment were of analytical purity grade from Merck (K2Cr2O7, NaOH, (NH4)2Fe(SO4)2, NH4Cl, HNO3) and from China (KCl, HCl, NaH2PO4, H2O2).

2.3.2. Preparation of biochar

Coffee husk biochar was prepared following a modified version of the method described by Yoo et al. [16]. In this adaptation, coffee husks were oven-dried at 60°C and crushed to a particle size below 5 mm. Then, it was pyrolyzed under anaerobic conditions in Nabertherm Furnace, Germany at a temperature of 300°C, with a heating rate programmed at 10°C/min, and stored for 2 h. The samples were then cooled naturally in the furnace overnight. The pyrolysis product was crushed through a 1-mm sieve and stored in a sealed PE bag at 4°C [16]. Biochar samples were then used to determine recovery efficiency (%H), pH, TOC, number of H+/OH- groups, and CEC. The results have been presented in detail in previous research [17] [Table 1].

Table 1: Recovery efficiency and some surface physicochemical components of biochar.

t °C%HpHmmolH+ g−1mmolOH– g−1%TOCCEC, cmol kg−1
30051.47.591.7311.1725.530.9
SD0.80.160.110.151.011

% H: Recovery efficiency. pzc: Point of zero charge. SD: Standard deviation.

2.3.3. Preparation of alkalized biochar

Accurately 30 g of biochar was weighed into a 500-mL beaker containing 200 mL of distilled water. The mixture was homogenized on the IKA T25 digital–Germany machine for 1 h at a speed of 6000 rpm. Small amounts of 85% CaO were added to the mixture to maintain pH 9–10. The samples were ground for another hour and pH was adjusted with H2SO4 and water so that the mixture weighed 300 g at pH 8. The samples were then transferred to plastic bottles, sealed tightly, and used for incubation experiments.

2.3.4. Incubation experiment

The soil incubation process under submerged conditions was simulated according to Maranguit et al. [18]. There are eight treatments as listed in Table 2.

Table 2: Treatments used in the experiment.

TreatmentsCombined MaterialsTreatmentsCombined Materials
V 0.00%Soil + 0.0% lime (control sample)VB 0.0%Soil + lime 0.40% + 0.0% alkaline biochar
V 0.40%Soil + 0.4% limeVB 0.2%Soil + lime 0.40% + 0.2% alkaline biochar
V 0.60%Soil + 0.6% limeVB 0.4%Soil + lime 0.40% + 0.4% alkaline biochar
V 1.00%Soil + 1.0% limeVB 0.8%Soil + lime 0.40% + 0.8% alkaline biochar

Specifically, 10 g of each soil sample according to the presented treatments was taken in a 60-mL polypropylene tube. A volume of 24 mL of pure distilled water was added to each tube (ensuring that there was always a layer of water on the surface), and the air was expelled by N2 degassing. Then, the suspension is covered with a rubber stopper to prevent O2 diffusion or loss due to evaporation and ensure anaerobic conditions. Three replicates for each determination were incubated at room temperature in the dark at 28 ± 1°C. After adding water, the samples were collected on days 7, 14, 21, 35, and 60 of submergence. The samples were shaken, filtered (filter with a pore size of 0.45 μm), and determined to identify pH, EC, available phosphorus, and NH4+ in the filtrate solution. The filtrate used to determine soluble P and NH4+ was immediately acidified with 1 mL of 0.1 M HCl to prevent microbiological processes.

2.4. Processing Experimental Data

Following data collection, all statistical analyses were performed using the Microsoft Excel software. To minimize bias, replicate samples were incorporated into the analyses, allowing for the evaluation of both precision and potential systematic errors. All experiments and subsequent analyses were repeated in triplicate for increased reliability. Homogeneity of variance was assessed using the SPSS 23.0 software. Subsequently, differences in mean values between experiments were determined (p-value < 0.05). For experiments with non-significant variance (Sig > 0.05), Tukey’s post-hoc test was used for multiple comparisons. Conversely, Tamhane’s T2 test was employed when significant variance was observed (Sig < 0.05).


3. RESULTS AND DISCUSSION

3.1. Determine the Physicochemical Composition of the Soil

The results of determining some physicochemical properties of soil samples collected in Giang Thanh, Kien Giang, Vietnam are presented in detail in Table 3. The soil sample obtained is of the loam clay type according to the soil texture triangle with clay, silt, and sand being 64%, 34%, and 2% of the Orthi Thionic Fluvisols soil type, mainly heavy sulfate alum soils. The results obtained are similar to the research of Ly et al. [19], also on soil in the Mekong Delta rice-growing area in terms of soil structure. The TOC content of 5.4% is similar to the research results of Khuong and colleagues stating that acid sulfate soil in the Mekong Delta ranges from 4.10% to 6.16% [20] or as studied by Ly et al. [19] from 1.4% to 6.1%.

Table 3: Physical and chemical parameters of the soil sample.

ParametersValueSD
pH3.610.02
EC (mS cm−1)0.570.02
Bulk density, g cm−31.120.02
Particle density, g cm−32.490.16
TOC, %5.40.1
Cation exchange capacity (CEC), cmol kg−128.60.6
Available phosphorus content, mg P.O. kg−111818
NH4+ content, mg kg−110.00.2
Clay, %64Loam clay
Silt, %34
Sand, %2

The bulk density value is 1.12 g cm−3, lower than that obtained by Ly et al. [19] (1.25 g cm−3), possibly due to the higher organic content of the research sample. The CEC of the Giang Thanh soil sample was 28.6 cmol kg−1, showing research results that were higher than pineapple soil in Hau Giang ranging from 9.5 to 15.0 cmol kg−1 [20].

Assessed according to the London 1984 scale from the report of Chiem et al. [21], the CEC value in the study was high (15.1–30.0 cmol kg−1). CEC is an important soil property because it affects the assimilation of nutrients and the acid-buffering capacity of the soil. Determination results [Table 3] show that pH 3.61 belongs to the group of strongly acidic soils, and only the most acid-tolerant plants can grow in this pH [22]. Similar results were also found with some acid sulfate soil compared to pineapple soil in Vi Thanh, Hau Giang, with pH ranging from 3.10 to 4.49 [20]. The EC value of the soil sample is 0.57 mS cm−1, which is also equivalent to pineapple soil in Hau Giang with EC ranging from 0.19 to 1.99 mS cm−1 [20] or as in the study on rice soil by Ly and colleagues, it was 0.56 mS cm−1 [19].

3.2. Effect of Lime on pH, EC, Releasing Phosphorus, and Ammonium Nitrogen

3.2.1. pH of soil solution

Research results on the influence of soil incubation time when adding lime at ratios of 0.0, 0.4, 0.6, and 1.0% to soil solution pH are presented in Figure 1. The results showed that pH increased rapidly in the first week for lime-added treatments. Specifically, pH increased from 3.6 to 6.9, 6.5, and 7.3 correspond to the amount of lime added increasing from 0.0, 0.4, 0.6, and 1.0%, respectively. In the following weeks, the pH fluctuated from 6.6 to 5.7 for a lime ratio of 0.4%, ranging from 6.1 to 7.0 when the rate is 0.6%, ranging from 7.3 to 7.4 with a rate of 1.0%. In particular, at the lime ratio of 0.4%, the pH showed signs of decreasing slightly over incubation time, while at dosages of 0.6 and 1.0%, the change is not statistically significant according to one-way ANOVA on SPSS 23.

Figure 1: Effect of soil incubation time on soil solution pH when using lime, the different letters of the same color in the graph indicate meaningful differences.



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Research results showed that pH decreased significantly on day 14 with lime rates of 0.4 and 0.6%. This can be explained by the possibility of reactions occurring between lime and protons that are inside the soil particles. The results are similar to the research of Mkhonza et al. and Thite et al. [1,23]. The decreased pH may be due to nitrification or decomposition of organic complexes Al and Fe in the soil [23]. Research results have shown that soil incubation time changed pH in case of increasing the addition of lime but in general, the change is insignificant for the soil sample. The results of the study are similar to the study of Mosharrof et al. [9] that lime addition increased soil solution pH.

3.2.2. EC of soil solution

Research results on the influence of soil incubation time when adding lime at ratios of 0.0, 0.4, 0.6, and 1.0% to EC soil solution were presented in Figure 2. Research results showed that EC did not change significantly from day 7 to day 21 for treatments adding lime at a rate of 0.0, 0.4, and 1.0% according to one-way ANOVA analysis on SPSS 23. Specifically, EC fluctuated from 0.62 to 0.63, 0.74 to 0.71, and 0.59 to 0.63 mS cm−1 corresponding to the proportions of lime added. With a lime ratio of 0.6%, the EC changed insignificantly on days 7 to 14, but on day 21, the change was statistically significant, specifically, EC was 0.57, 0.59, and 0.68 mS cm−1, respectively. Thereafter, there was a statistically significant increasing trend up to day 35 for all treatments, corresponding to EC values of 0.69, 0.88; 0.73, and 0.75 mS cm−1. The increase in EC can be explained by mineralization and decomposition of organic fertilizers, which release ions into the soil solution and cause EC to increase [2]. When incubation continued until day 60, the trend decreased slowly for all treatments, but the decrease was not significant, corresponding to EC values of 0.68, 0.82, 0.710, and 0.715 mS cm−1. The reduction process can be explained by the precipitation of dissolved P minerals in the form of Al and Fe bonds [23]. This explanation is also completely consistent with the results of the study showing that the amount of P in the soil solution decreased sharply at day 60 [Figure 3]. The results of the study are contrary to the results of the study by Thite et al. [1], which showed an increase in EC values at day 60. This can be explained by the soil characteristics in the study being acidic soil with a pH (of 3.6) and high TOC content (5.4%) compared to the soil in Thite et al.’s [1] study, which had high pH (8.1) and low TOC (0.57%). When organic content is decomposed, Al and Fe ions will be released in acidic soils, which will fix a large amount of P in the soil solution in the form of precipitation or surface adsorption.

Figure 2: Effect of soil incubation time on soil solution EC when using lime, the different letters of the same color in the graph differ significantly.



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Figure 3: Effect of soil incubation time on released P when using lime, the different letters of the same color in the graph differ significantly.



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3.2.3. Release of phosphorus

Results of surveying the effect of soil incubation time on the P release process in the soil at lime addition ratios of 0.0, 0.4, 0.6, and 1.0% were presented in Figure 3. The results showed that the P release content on day 7 of the control soil sample was at the lowest level of 17.8 mg kg−1 compared to 58.5, 71.6, and 79.1 mg kg−1 corresponding to a lime ratio of 0.4, 0.6, and 1.0%. After that, it continued to maintain a stable high level until day 35, specifically the amount of P released was 80.6 and 72.2 mg kg−1 corresponding to lime ratios of 0.4 and 0.6%. While at lime ratios of 1.0%, phosphorus release decreased insignificantly on days 7 and 14; but significantly decreased sharply on days 14 and 21. Specifically, the amount of phosphorus released was 79.1, 74.2 to 57.0 mg kg−1. After day 35, the process continued to decrease until day 60, with a significant decrease for treatments with a dose of 0.0, 0.4, and 0.6% lime. However, 1.0% lime treatment had a significant decrease on day 35, and the decrease was insignificant compared to day 60. The increase in P content in the soil solution with lime additions in acidic soils can be explained by the fact that lime increases the pH of the soil solution, seriously weakening the activity of Al oxide, reducing the ability to retain P of the oxide surface, and thus increasing P availability. A similar explanation was also found in the study of Mosharrof et al. [9]. However, using high amounts of lime (in the case of adding 1.0% lime) will precipitate P with Ca in the form of calcium phosphate [24]. Similar results were revealed by Nishida et al. [25].

In the case of a sharp decrease in the amount of P released at days 35 and 60, it can be explained by the fact that the addition of lime increases the microbial community, thus increasing the decomposition of organic matter and the release of Fe and Al. At that time, the lowest P content is due to the strongest bond between P and Al and Fe. The research results are similar to the study of Mkhonza et al. [23].

3.2.4. Release of NH4+

Results of surveying the effect of soil incubation time on the release of NH4+ in soil with lime addition ratios of 0.0, 0.4, 0.6, and 1.0% are presented in Figure 4. The results showed that NH4+ release increased on days 7 and 14 but was not statistically significant in treatments with lime ratio 0.0, 0.6, and 1.0%, specifically the amount of NH4+ on days 7 and 14 ranged from 0.9 to 1.2, 1.3 to 1.2, and 0.3 to 0.4 mg kg−1, respectively. Meanwhile, at a lime ratio of 0.4%, the amount of NH4+ released increased significantly, specifically from 0.3 to 1.8 mg kg−1 on days 7 and 14, respectively. The amount of NH4+ continued to increase on day 21, and the increase was statistically significant in all experimental treatments. Then, it decreased on day 35, and the decrease was significant with treatments using lime ratios of 0.0, 0.4, and 0.6%, while with the 1.0% lime treatment, there was no significant decrease. Next was the increase again on day 60, the increase was statistically significant for all experimental treatments. This can be explained because the increased amount of lime added increased pH, which increased microbial activity, leading to increased ability to decompose SOM. Then, it is possible to increase the amount of mineral N [23]. In case the amount of NH4+ released decreases, it may be due to an increase in nitrification from microbial activity. The increase or decrease in the amount of NH4+ released does not follow a clear trend, possibly because the activity of microorganisms in the soil depends on many factors such as pH, organic matter content, and soil type [23]. Therefore, specific research is needed.

Figure 4: Effect of soil incubation time on released NH4+ when using lime, the different letters of the same color in the graph differ significantly.



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In general, the use of lime in improving acidic soil samples has improved pH and EC and improved the maintenance of released phosphorus until day 21 ranging from 57.0 to 80.6 mg kg−1 but did not improve the amount of NH4+ released, only ranging from 0.4 to 3.0 mg kg−1.

3.3. Effect of Combined Addition of Lime and Alkalinized Biochar on pH, EC, Releasing Phosphorus, and Ammonium Nitrogen

3.3.1. pH of soil solution

Research results on the influence of soil incubation time when adding lime (ratio 0.4%) combined with alkalized biochar at 0.0, 0.2, 0.4, and 0.8% ratios on the pH of the soil solution were presented in Figure 5. The results show that the samples supplemented with alkaline biochar all had pH values greater than the control sample (no alkalized biochar added) during incubation. The pH trend decreased on day 7, the decrease ranged from 6.9 to 6.2, 7.7 to 6.5, 7.9 to 7.5, and 7.7 to 6.4, corresponding to alkalinized biochar ratios of 0.0, 0.2, 0.4, and 0.8%. The decrease in pH levels on days 7 and 21 was statistically significant for all treatments supplemented with alkalinized biochar. The decrease in pH level on days 21 and 60 was statistically significant with the treatment adding 0.2 and 0.8% alkalinized biochar, while the 0.4% rate was not significant, according to the results of one-way ANOVA analysis on SPSS 23.

Figure 5: Effect of soil incubation time on pH when using lime (0.4%) combined with alkalized biochar, the different letters of the same color in the graph differ significantly.



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The decrease in pH may be due to the release of organic acids such as humic acid, carbonic acid, and other organic acids during the mineralization of organic matter [2]. The research results are also similar to the study of Thite et al. [1], which suggested that organic matter caused the pH decrease during incubation. Furthermore, the prolonged decrease in pH over time can also be explained by the release of aluminum from the organic Al complex during the decomposition of organic matter of biochar. Similar explanations were also found in the study by Mkhonza et al. [23].

3.3.2. EC of soil solution

Research results on the effects of soil incubation time when adding lime (0.4%) combined with alkalized biochar at ratios of 0.0, 0.2, 0.4, and 0.8% on soil solution EC were presented in Figure 6. Research results showed that the EC values of the treatments supplemented with alkalized biochar were all smaller than the control samples on day 7. When increasing the biochar ratio, the EC value increases. Specifically, the EC value increased by 0.55, 0.62, and 0.69 dS m−1, respectively [Figure 6]. This can be explained by the increase in soluble salts that are present in alkalinized biochar. The EC value increased rapidly and significantly from day 7 to day 14 for the treatment adding 0.2 and 0.4% alkalinized biochar and maintained a non-significant change until day 35. Then, there was a significant decrease at day 60. Meanwhile, the EC value of the VB 0.0 treatment did not change significantly on days 7, 14, and 21 and only increased significantly at day 35 and no significant change at day 60. With the VB 0.8% treatment, the EC value fluctuated the least (0.69–0.73 dS m−1), not meaningful, according to one-way ANOVA analysis on SPSS 23.

Figure 6: Effect of soil incubation time on EC when using lime (0.4%) combined with alkalized biochar, the different letters of the same color in the graph differ significantly.



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The increase in EC can be explained by the mineralization and decomposition of organic matter, which releases ions into the soil solution, leading to an increase in soil EC. Similar explanations were also found in the study by Patra et al. [2]. Furthermore, Ca ions can replace monovalent cations on the soil surface and also contribute to increasing EC in the soil solution. Similar explanations were found in the study by Nigussie et al. [26]. The decrease in EC at day 60 may be due to a decrease in the solubility of Al and Fe in the soil when binding with available P to form precipitation. A similar explanation is also found in the report by Nigussie et al. [26].

3.3.3. Release of phosphorus

Results of surveying the effect of soil incubation time on the P release process in soil supplemented with lime (0.4%) combined with alkaline biochar at ratios of 0.0, 0.2, 0.4, and 0.8 % were presented in Figure 7. The released P content remained high during the first 3 weeks (up to day 21), for treatments supplemented with alkaline biochar. Specifically, for treatment VB 0.2%, the amount of P released fluctuated from 62.4 to 62.4 mg kg−1, for treatment VB 0.4%, the level fluctuated from 76.6 to 64.6, and for treatment VB 0.8% levels ranged from 68.8 to 80.3 mg kg−1.

Figure 7: Effect of soil incubation time on released P when using lime (0.4%) combined with alkalized biochar, the different letters of the same color in the graph differ significantly.



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This can be explained by the fact that organic acid salts in biochar may compete for P adsorption sites or, if previously adsorbed, P may be ejected. Similar explanations were also found in the study by Fink et al. [27]. The organic salts present in biochar have a high affinity for Al and Fe, and this affinity allows for the removal of Al and Fe, leading to the release of free P from these bonds [24].

Then, the amount of released P decreased significantly at day 35 in the case of treatments VB 0.4 and VB 0.8%, corresponding to the amount of P released as 21.2 and 16.0 mg kg−1. Only in the case of the VB 0.2% treatment was maintained until day 35, the change was not significant with a released p-value of 70.5 mg kg−1. The amount of released P decreased sharply at day 60, the decrease was significant with the VB 0.2% treatment and insignificant with the VB 0.4 and VB 0.8% treatments. This can be explained by resorption or precipitation on the soil surface and by precipitation of Al and Fe with P from the decomposition of organic matter of Al and Fe complexes [28]. Furthermore, it can also be attributed to the fixation of P in the form of tricalcium phosphate. Similar results were found by Nishida et al. [25].

3.3.4. Release of NH4+

Results of surveying the effect of soil incubation time on the release of NH4+ in soil supplemented with lime (0.4%) combined with alkalized biochar at ratios of 0.0, 0.2, 0.4, and 0.8% were presented in Figure 8. The released NH4+ content remained at a higher level than the control treatment VB 0, at most soil incubation times. The amount of NH4+released increased on day 7 of incubation and remained high until day 21, ranging from 19.3 to 23.2, 20.7 to 22.4, and 20.1 to 22.7 mg kg−1 correspond to treatments of VB 0.2, VB 0.4, and VB 0.8. Then it decreased sharply with a significant decrease at day 35, respectively, 7.0, 11.5, and 13.3 mg kg−1. Then it decreased slowly at day 60, fluctuating around a value of 8.0 mg kg−1.

Figure 8: Effect of soil incubation time on released NH4+ when using lime (0.4%) combined with alkalized biochar, the different letters of the same color in the graph differ significantly.



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Increased available NH4+ content in the soil solution may be due to increased microbial communities leading to the mineralization of organically bound N. This can also be attributed to the decrease in soil pH increasing NH4+ in the soil [25]. Furthermore, Ca cations contained in alkaline biochar also contribute to removing NH4+ from adsorption sites in the soil. In addition, dissolved or colloidal organic anions in alkaline biochar also contribute to attracting NH4+ in the soil. The results obtained were similar to the study of Liyanage et al. [29] when investigating the NH4+ mineralization process using green manure, which occurred within about 7–20 days of incubation and was higher than using biochar directly.

The decrease in NH4+ content may be due to NH4+ fixation in wetland soil. Microbial Fe3+ reduction, followed by the dissolution of Fe oxides coating the clay mineral surface, promotes the diffusion of NH4+ ions into the interlayers of the clay mineral. A similar explanation is also found in the report of Nieder et al. [6]. On the other hand, after 30 days the nitrification process also increased rapidly, with the amount of NH4+ converted to NO3 reducing the NH4+ content, the results are similar to the study by Liyanage et al. [29].

The use of lime combined with alkalinized biochar as an acidic soil amendment on the collected sample in Vietnam has improved pH and EC, and maintained high phosphorus release until day 35, fluctuating at 59.0–80.0 mg kg−1 and maintained high NH4+ release ranging from 19 to 23 mg kg−1 until day 21.


4. CONCLUSION

From the above research results, it allows us to draw some conclusions as follows:

Soil samples at geographical coordinates 10°24′34.7′′N 104°45′34.0′′E in Vinh Phu, Giang Thanh, Kien Giang, Vietnam were collected and submerged under controlled conditions in the laboratory. The results showed that the incubation time did not affect pH and EC but affected available phosphorus (released phosphorus remains at a high level for a period of 7–21 days with samples V 0.4% and V 0.6%), while NH4+ release increased on day 21 and decreased on day 35 in the case of incubation with lime. While the solution of using lime (0.4%) combined with alkaline biochar not only maintains pH and EC, it also increases available phosphorus and NH4+ in the soil solution for a longer period (35 days). Research results showed that incubation time changed pH, EC, available phosphorus, and ammonium nitrogen in the soil sample but did not follow a clear trend. The study results also provide valuable insights into soil incubation times for developing strategies for using biochar-based products.


5. ACKNOWLEDGMENTS

This research was supported by students of course 15, Institute of Science, Engineering and Environmental Management. We thank the reviewers for their comments to improve the quality of the manuscript.


6. AUTHOR CONTRIBUTIONS

All authors made substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; took part in drafting the article or revising it critically for important intellectual content; agreed to submit to the current journal; gave final approval of the version to be published; and agree to be accountable for all aspects of the work. All the authors are eligible to be an author as per the international committee of medical journal editors (ICMJE) requirements/guidelines.


7. FINANCIAL SUPPORT AND SPONSORSHIP

There is no funding to report.


8. CONFLICTS OF INTEREST

The authors report no financial or any other conflicts of interest in this work.


9. ETHICAL APPROVALS

This study does not involve experiments on animals or human subjects.


10. DATA AVAILABILITY

All the data is available with the authors and shall be provided upon request.


11. USE OF ARTIFICIAL INTELLIGENCE (AI)-ASSISTED TECHNOLOGY

The authors confirm that there was no use of artificial intelligence (AI)-assisted technology for assisting in the writing or editing of the manuscript and no images were manipulated using AI.


12. PUBLISHER’S NOTE

All claims expressed in this article are solely those of the authors and do not necessarily represent those of the publisher, the editors and the reviewers. This journal remains neutral with regard to jurisdictional claims in published institutional affiliation.


REFERENCES

1.  Thite MD, Ingle SR, Gaikwad AS. Effect of incubation duration of incorporated organic manures on chemical properties of Inceptisol. Pharma Innov J. 2021;11(1):162–6.

2.  Patra A, Sharma VK, Purakayastha TJ, Barman M, Kumar S, Chobhe KA, et al. Effect of long-term integrated nutrient management (INM) practices on soil nutrients availability and enzymatic activity under acidic Inceptisol of North-Eastern region of India. Commun Soil Sci Plant Anal. 2020;51(9):1137–49.

3.  Olowoboko TB, Azeez JO, Olujimi OO, Babalola OA. Comparative evaluation of animal manures and their ashes on soil pH and electrical conductivity in some Southwestern Nigerian soils. Commun Soil Sci Plant Anal. 2018;49(12):1442–54.

4.  Debicka M, Kocowicz A, Weber J, Jamroz E. Organic matter effects on phosphorus sorption in sandy soils. Arch Agron Soil Sci. 2016;62:840–55.

5.  Han Y, Choi B, Chen X. Adsorption and desorption of phosphorus in biochar-amended black soil as affected by freeze-thaw cycles in northeast China. Sustainability. 2018;10(5):1574.

6.  Nieder R, Benbi DK, Scherer HW. Fixation and defixation of ammonium in soils: a review. Biol Fert Soils. 2011;47:1–14.

7.  Hui TR, Park E, Loc HH, Tien PD. Long-term hydrological alterations and the agricultural landscapes in the Mekong Delta: insights from remote sensing and national statistics. Environ Chall. 2022;7:100454.

8.  Papageorgiou A, Sinha R, Azzi ES, Sundberg C, Enell A. The role of biochar systems in the circular economy: biomass waste valorization and soil remediation. Circ Econ. 2022.

9.  Mosharrof M, Uddin MK, Jusop S, Sulaiman MF, Shamsuzzaman S, Haque ANA. Changes in acidic soil chemical properties and carbon dioxide emission due to biochar and lime treatments. Agriculture. 2021;11(3):219.

10.  Uddin S, Islam MR, Jahangir MMR, Rahman MM, Hassan S, Hassan MM, et al. Nitrogen release in soils amended with different organic and inorganic fertilizers under contrasting moisture regimes: a laboratory incubation study. Agronomy. 2021;11(11):2163.

11.  Nguyen BT, Nguyen VN, Nguyen TX, Nguyen MH, Dong HP, Dinh GD, et al. High biochar rates may suppress rice (Oryza sativa) growth by altering the ratios of C to N and available N to P in paddy soils. Soil Use Manage. 2023;39(1):415–28.

12.  Kumar A, Joseph S, Graber ER, Taherymoosavi S, Mitchell DR, Munroe P, et al. Fertilizing behavior of extract of organomineral-activated biochar: low-dose foliar application for promoting lettuce growth. Chemical and Biol Technol Agricult. 2021;8:1–15.

13.  Trung, B. Continuously exporting large batches of coffee is a good sign for Dak Lak. 2024 [cited 2024 March 10]. Available from: https://www.vietnam.vn/en/lien-tuc-xuat-khau-nhung-lo-ca-phe-lon-la-tin-hieu-dang-mung-cho-dak-lak/

14.  Tan KH. Soil sampling, preparation, and analysis. Boca Raton, FL: CRC Press; 2005.

15.  Ahmadi F. Kinetics of phosphorus release in soils with different textures in intensive cultivation systems. J Earth Sci Clim Change. 2018;9(1):1–4.

16.  Yoo G, Kim H, Chen J, Kim Y. Effects of biochar addition on nitrogen leaching and soil structure following fertilizer application to rice paddy soil. Soil Sci Soc Am J. 2014;78(3):852–60.

17.  Phuong NV, Hoang NK, Luan LV, Tan L. Evaluation of NH4+ adsorption capacity in water of coffee husk-derived biochar at different pyrolysis temperatures. Int J Agron. 2021;2021:1–9.

18.  Maranguit D, Guillaume T, Kuzyakov Y. Effects of flooding on phosphorus and iron mobilization in highly weathered soils under different land-use types: short-term effects and mechanisms. Catena. 2017;158:161–70.

19.  Ly NTH, Phuong NT, Anh LN, Nguyet DA, Nguyen H. Effect of agricultural land-use patterns on soil organic carbon stock in the upper Vietnamese mekong delta. Polish J Environ Stud. 2022;31(6):5793–804. [CrossRef]

20.  Khuong NQ, Vi LLV, Linh TB, Thuc LV, Toan LP, Nguyen PC, et al. Morphological, chemical and physical characteristics of alum soil profile for pineapple cultivation in Vi Thanh City, Hau Giang Province. Can Tho University J Sci. 2020;56:88–97.

21.  Chiem NH, Khanh HC, Loc NX, Huynh DTV. Evaluation and comparison of physical-chemical properties of rice-cultivated soil in and outside the closed dyke in An Giang province. Can Tho University J Sci. 2017;1:86–92.

22.  Amacher MC, O’Neill KP, Perry CH. Soil vital signs: a new soil quality index (SQI) for assessing forest soil health. Res. Pap. RMRS-RP-65WWW. 2007;1–12. [CrossRef]

23.  Mkhonza N, Buthelezi-Dube N, Muchaonyerwa P. Effects of lime application on nitrogen and phosphorus availability in humic soils. Sci Rep. 2020;10(1):8634.

24.  Ch’ng HY, Ahmed OH, Majid NMA. Improving phosphorus availability in an acid soil using organic amendments produced from agroindustrial wastes. Sci World J. 2014;2014:506356. [CrossRef]

25.  Nishida M, Sekiya H, Yoshida K. Status of paddy soils as affected by paddy rice and upland soybean rotation in northeast Japan, with special reference to nitrogen fertility. Soil Sci Plant Nutr. 2015;59(2):208–17.

26.  Nigussie A, Kissi E, Misganaw M, Ambaw G. Effect of biochar application on soil properties and nutrient uptake of lettuces (Lactuca sativa) grown in chromium polluted soils. Am Eur J Agricult Environ Sci. 2012;12(3):369–76.

27.  Fink JR, Inda AV, Tiecher T, Barrón V. Iron oxides and organic matter on soil phosphorus availability. Ciencia Agrotecnol. 2016;40:369–79.

28.  Najafi N. Changes in pH, EC and concentration of phosphorus in soil solution during submergence and rice growth period in some paddy soils of North of Iran. Int J Agricult Res Rev. 2013;3(2):271–80.

29.  Liyanage M, Hanafi MM, Sulaiman MF, Ismail R, Gunaratne G, Dharmakeerthi S, et al. Consequences of nitrogen mineralization dynamics for soil health restoration of degraded tea-growing soil using organic amendments. Chilean J Agricult Res. 2022;82(2):199–210.

Reference

1. Thite MD, Ingle SR, Gaikwad AS. Effect of incubation duration of incorporated organic manures on chemical properties of Inceptisol. Pharma Innov J. 2021;11(1):162-6. https://doi.org/10.22271/tpi.2022.v11.i1c.9950

2. Patra A, Sharma VK, Purakayastha TJ, Barman M, Kumar S, Chobhe KA, et al. Effect of long-term integrated nutrient management (INM) practices on soil nutrients availability and enzymatic activity under acidic Inceptisol of North-Eastern region of India. Commun Soil Sci Plant Anal. 2020;51(9):1137-49. https://doi.org/10.1080/00103624.2020.1751185

3. Olowoboko TB, Azeez JO, Olujimi OO, Babalola OA. Comparative evaluation of animal manures and their ashes on soil pH and electrical conductivity in some Southwestern Nigerian soils. Commun Soil Sci Plant Anal. 2018;49(12):1442-54. https://doi.org/10.1080/00103624.2018.1464184

4. Debicka M, Kocowicz A, Weber J, Jamroz E. Organic matter effects on phosphorus sorption in sandy soils. Arch Agron Soil Sci. 2016;62:840-55. https://doi.org/10.1080/03650340.2015.1083981

5. Han Y, Choi B, Chen X. Adsorption and desorption of phosphorus in biochar-amended black soil as affected by freeze-thaw cycles in northeast China. Sustainability. 2018;10(5):1574. https://doi.org/10.3390/su10051574

6. Nieder R, Benbi DK, Scherer HW. Fixation and defixation of ammonium in soils: a review. Biol Fert Soils. 2011;47:1-14. https://doi.org/10.1007/s00374-010-0506-4

7. Hui TR, Park E, Loc HH, Tien PD. Long-term hydrological alterations and the agricultural landscapes in the Mekong Delta: insights from remote sensing and national statistics. Environ Chall. 2022;7:100454. https://doi.org/10.1016/j.envc.2022.100454

8. Papageorgiou A, Sinha R, Azzi ES, Sundberg C, Enell A. The role of biochar systems in the circular economy: biomass waste valorization and soil remediation. Circ Econ. 2022. https://doi.org/10.5772/intechopen.104389

9. Mosharrof M, Uddin MK, Jusop S, Sulaiman MF, Shamsuzzaman S, Haque ANA. Changes in acidic soil chemical properties and carbon dioxide emission due to biochar and lime treatments. Agriculture. 2021;11(3):219. https://doi.org/10.3390/agriculture11030219

10. Uddin S, Islam MR, Jahangir MMR, Rahman MM, Hassan S, Hassan MM, et al. Nitrogen release in soils amended with different organic and inorganic fertilizers under contrasting moisture regimes: a laboratory incubation study. Agronomy. 2021;11(11):2163. https://doi.org/10.3390/agronomy11112163

11. Nguyen BT, Nguyen VN, Nguyen TX, Nguyen MH, Dong HP, Dinh GD, et al. High biochar rates may suppress rice (Oryza sativa) growth by altering the ratios of C to N and available N to P in paddy soils. Soil Use Manage. 2023;39(1):415-28. https://doi.org/10.1111/sum.12842

12. Kumar A, Joseph S, Graber ER, Taherymoosavi S, Mitchell DR, Munroe P, et al. Fertilizing behavior of extract of organomineral-activated biochar: low-dose foliar application for promoting lettuce growth. Chemical and Biol Technol Agricult. 2021;8:1-15. https://doi.org/10.1186/s40538-021-00222-x

13. Trung, B. Continuously exporting large batches of coffee is a good sign for Dak Lak. 2024 [cited 2024 March 10]. Available from: https://www.vietnam.vn/en/lien-tuc-xuat-khau-nhung-lo-ca-phe-lon-la-tin-hieu-dang-mung-cho-dak-lak/

14. Tan KH. Soil sampling, preparation, and analysis. Boca Raton, FL: CRC Press; 2005.

15. Ahmadi F. Kinetics of phosphorus release in soils with different textures in intensive cultivation systems. J Earth Sci Clim Change. 2018;9(1):1-4. https://doi.org/10.4172/2157-7617.1000441

16. Yoo G, Kim H, Chen J, Kim Y. Effects of biochar addition on nitrogen leaching and soil structure following fertilizer application to rice paddy soil. Soil Sci Soc Am J. 2014;78(3):852-60. https://doi.org/10.2136/sssaj2013.05.0160

17. Phuong NV, Hoang NK, Luan LV, Tan L. Evaluation of NH_4^+ adsorption capacity in water of coffee husk-derived biochar at different pyrolysis temperatures. Int J Agron. 2021;2021:1-9. https://doi.org/10.1155/2021/1463814

18. Maranguit D, Guillaume T, Kuzyakov Y. Effects of flooding on phosphorus and iron mobilization in highly weathered soils under different land-use types: short-term effects and mechanisms. Catena. 2017;158:161-70. https://doi.org/10.1016/j.catena.2017.06.023

19. Ly NTH, Phuong NT, Anh LN, Nguyet DA, Nguyen H. Effect of agricultural land-use patterns on soil organic carbon stock in the upper Vietnamese mekong delta. Polish J Environ Stud. 2022;31(6):5793- 804. https://doi.org/10.15244/pjoes/152029

20. Khuong NQ, Vi LLV, Linh TB, Thuc LV, Toan LP, Nguyen PC, et al. Morphological, chemical and physical characteristics of alum soil profile for pineapple cultivation in Vi Thanh City, Hau Giang Province. Can Tho University J Sci. 2020;56:88-97.

21. Chiem NH, Khanh HC, Loc NX, Huynh DTV. Evaluation and comparison of physical-chemical properties of rice-cultivated soil in and outside the closed dyke in An Giang province. Can Tho University J Sci. 2017;1:86-92.

22. Amacher MC, O'Neill KP, Perry CH. Soil vital signs: a new soil quality index (SQI) for assessing forest soil health. Res. Pap. RMRS-RP-65WWW. 2007;1-12. https://doi.org/10.2737/RMRS-RP-65

23. Mkhonza N, Buthelezi-Dube N, Muchaonyerwa P. Effects of lime application on nitrogen and phosphorus availability in humic soils. Sci Rep. 2020;10(1):8634. https://doi.org/10.1038/s41598-020-65501-3

24. Ch'ng HY, Ahmed OH, Majid NMA. Improving phosphorus availability in an acid soil using organic amendments produced from agroindustrial wastes. Sci World J. 2014;2014:506356. https://doi.org/10.1155/2014/506356

25. Nishida M, Sekiya H, Yoshida K. Status of paddy soils as affected by paddy rice and upland soybean rotation in northeast Japan, with special reference to nitrogen fertility. Soil Sci Plant Nutr. 2015;59(2):208-17. https://doi.org/10.1080/00380768.2012.762588

26. Nigussie A, Kissi E, Misganaw M, Ambaw G. Effect of biochar application on soil properties and nutrient uptake of lettuces (Lactuca sativa) grown in chromium polluted soils. Am Eur J Agricult Environ Sci. 2012;12(3):369-76.

27. Fink JR, Inda AV, Tiecher T, Barrón V. Iron oxides and organic matter on soil phosphorus availability. Ciencia Agrotecnol. 2016;40:369-79. https://doi.org/10.1590/1413-70542016404023016

28. Najafi N. Changes in pH, EC and concentration of phosphorus in soil solution during submergence and rice growth period in some paddy soils of North of Iran. Int J Agricult Res Rev. 2013;3(2):271-80.

29. Liyanage M, Hanafi MM, Sulaiman MF, Ismail R, Gunaratne G, Dharmakeerthi S, et al. Consequences of nitrogen mineralization dynamics for soil health restoration of degraded tea-growing soil using organic amendments. Chilean J Agricult Res. 2022;82(2):199-210. https://doi.org/10.4067/S0718-58392022000200199

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Zainab Adenike Ayinla, Adedeji Nelson Ademakinwa, Femi Kayode Agboola

Phenotypic and genotypic diversity of Xanthomonas axonopodis pv. manihotis causing bacterial blight disease of cassava in Kenya

Mary N. Chege , Fred Wamunyokoli, Joseph Kamau, Evans N. Nyaboga

A comparative study of tree species composition of Panjhora forest beat and Sipchu forest beat of Chalsa forest range, West Bengal, India

Anup Kumar Sarkar, Manas Dey, Mallika Mazumder

In vitro antagonistic activity of a root endophytic fungus towards plant pathogenic fungi

K. Talapatra, A. Roy Das, A. K. Saha, P. Das

Impact of single visit of Lipotriches collaris Vachal 1903 (Hymenoptera: Halictidae) on Phaseolus vulgaris (Fabaceae) flowers at Maroua (Cameroon)

Chantal Douka, Joseph Lebel Tamesse, Fernand-Nestor Fohouo tchuenguem

Knock down efficacy of commercially available insecticides against Anopheles gambiae

Nicholas K. Makworo , Vincent O. Ochieng, Dorington O. Ogoyi, Richard W. Mukabana

Antibiotics susceptibility profile of Staphylococcus aureus isolated from selected hospital and non-hospital fomites

Oluwatayo Ayotunde Makinde, Ayorinde Bunmi Akinbobola, Olubunmi Olowokanga

Assessment of genetic diversity in Shorea robusta: an economically important tropical tree species

Giridara-Kumar Surabhi, Subhankar Mohanty, Rajesh Kumar Meher, Arup Kumar Mukherjee, Lakshmi Narayana R.Vemireddy

Effect of extraction solvents on phenolic contents and antioxidant capacities of Artocarpus chaplasha and Carissa carandas fruits from Bangladesh

Gouri Dhar, Sonam Akther, Afrin Sultana, Uchen May, Mohammed Moinul Islam, Mowri Dhali, Dwaipayan Sikdar

Morphological and Elemental analysis of the effluent of Lead-acid battery manufacturing

Narasimhulu Korrapati, Paramjeet Saroj, Nisha Gaur

In Vitro Antidiabetic and Anti-oxidant Activities of Methanol Extract of Tinospora Sinensis

Anindita Banerjee, Bithin Maji, Sandip Mukherjee, Kausik Chaudhuri, Tapan Seal

Effect of growth regulators on callus morphology of Rice anther culturec

Avinash Sharma, Dalpat Lal and Monoj Sutradhar

Polyphenol oxidase (PPO) based biosensors for detection of phenolic compounds: A Review

Ijaz Gul, M. Sheeraz Ahmad, S. M. Saqlan Naqvi, Ansar Hussain, Rahmat Wali, Ammad Ahmad Farooqi, Ibrar Ahmed

Practiced Gram negative bacteria from dyeing industry effluents snub metal toxicity to survive

Channarayapatna-Ramesh Sunilkumar , Lobo Rachel, Gurulingaiah Bhavya, Kujur Swati , R. Samaga Sridhar, J. Samanth Kumar, K. Ramachandra Kini, H. S. Prakash, Nagaraja Geetha

In vitro efficacy of different growth media and crude plant extracts against mycelia growth of Phytophthora capsici

M. Mohsan, S. Ali, M. Umar Shahbaz, Saba Saeed, M. Burhan

Genetic drift in six cultivated populations of Terminalia arjuna

Pramod Kumar Sairkar, Shweta Chouhan, Amit Sen, Rajesh Sharma, Raj Kumar Singh

Production, characterization and optimization of fermented tomato and carrot juices by using Lysinibacillus sphaericus isolate

Naga Sivudu Seelam, Harika Akula, Umamahesh Katike, Vijaya Sarathi Reddy Obulam

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

Mohamed Ali Abdel-Rahman, Said El-Sayed Desouky, Mohamed Salah Azab, Mahmoud E. Esmael

Evaluation of salt tolerance ability in some fig (Ficus carica L.) cultivars using tissue culture technique

Hemaid Ibrahim Ahemaidan Soliman, Mohamed R. A. Abd Alhady

Nonconventional induction strategies for production of recombinant human tumor necrosis factor-alpha in Escherichia coli

Tapan Kumar Singha, Pooja Gulati, Sanjay Kumar

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

RS Singh, Navpreet Kaur

Biodiversity and biotechnological applications of halophilic microbes for sustainable agriculture

Ajar Nath Yadav, Anil Kumar Saxena

Rapid and sensitive method for detection of Staphylococcus aureus enterotoxin genes in milk sample

Mahantesh M Kurjogi, Basappa B Kaliwal

Management of heat stress in Drosophila melanogaster with Abhrak bhasma and ascorbic acid as antioxidant supplements

Rambhadur P. Subedi, Rekha R. Vartak, Purushottam G. Kale

Insecticidal effect of four plant essential oils against two aphid species under laboratory conditions

Akram Saleh Alghamdi

Simplified detection of the asymmetric polymerase chain reaction-amplified DNA and its application in the target identification

G Suhasa, Savithri Bhat

Characterization of extracellular amylase from Bacillus sp. strain RU1

Aleem Basha Pinjari, Vijayalakshmi Kotari

Bio-prospecting of marine-derived fungi with special reference to production of keratinase enzyme - A need-based optimization study

P. Samuel, M. Maheswari, J. Vijayakumar, T. Selvarathinam, K. Amirtharaj, R. Deenathayalan

Optimization of the conditions for rice bran phytate degradation by their own phytases

Abd-El Aziem Farouk, N. Thoufeek Ahamed, Anis Shobirin Meor Hussin , Othman Al Zahrani, Saqer Alotaibi

Cold-active enzymes in food biotechnology: An updated mini review

Mohammed Kuddus

In vitro conservation and propagation of the endemic species of “floating hearts” (Nymphoides krishnakesara Joseph and Sivar. - Menyanthaceae) as a conservation strategy

Swetha Thilak T, Anusree P, Saranya Babu Jayaprakash CM, Smitha RB, Minoo Divakaran, Madhusoodanan PV, Prakashkmar R

Isolation and characterization of denitrifying halophilic bacteria from Bahr Al-Milh Salt Lake, Karbala, Iraq

Maryam Hosseini, Mushtaq T Sh. Al-Rubaye, Javad Fakhari, Fateme Babaha

Comprehensive analysis of damage associated SNPs of Sex Hormone Binding Globulin gene

Richa Bhatnager, Mehak Dangi, Amita Suneja Dang

Submerged fermentation, purification, and characterization of L-asparaginase from Streptomyces sp. isolated from soil

Savitha S. Desai, Basavaraj S. Hungund

Evaluating the efficacy of six novel indigenous free-living soil bacteria on tea plant of North Bengal tea gardens of West Bengal India and their antagonistic effect on some tea pathogenic fungi

Jayanta Bhaduri, Subhash Kanti Roy

Aroclor 1254 induced oxidative stress and histopathological changes in mice liver

Jalpa Raja, Shweta Pathak, Rahul Kundu

Bioconversion of sugarcane molasses to poly(3-hydroxybutyrate-co-3- hydroxyvalerate) by endophytic Bacillus cereus RCL 02

Rituparna Das, Arundhati Pal, Amal Kanti Paul

Phytoextraction of Heavy Metals and Risk Associated with Vegetables Grown from Soil Irrigated with Refinery Wastewater

A. Y. Ugya, A. M. Ahmad, H. I. Adamu, S. M. Giwa, T. S. Imam

In vitro propagation of chia (Salvia hispanica L.) and assessment of genetic fidelity using random amplified polymorphic DNA and intersimple sequence repeat molecular markers

Anita Yadav, S L Kothari, Sumita Kachhwaha, Anuja Joshi

Consequence of chromium-tainted soil on physical and biochemical responses of Vigna radiata L.

Bibhu Prasad Rath, Sujata Hota, Subhra Subhadarshini, Debasis Dash, Prabhu Kaibalya Das

A study of endophytic fungi Neofusicoccum ribis from Gandaria (Bouea macrophylla Griffith) as enzyme inhibitor, antibacterial, and antioxidant

Trisanti Anindyawati, Praptiwi

Morphological Trait, Molecular Genetic Evidence and Proteomic Determination of Different Chickens (Gallus gallus) Breeds

Wirot Likittrakulwong, Pisit Poolprasert, Sittiruk Roytrakul

Hypolipidemic effects of Lysinibacillus sphaericus fermented tomato and carrot juices in high-fat diet-fed albino Wistar rats

Naga Sivudu Seelam, Umamahesh Katike, Peddanna Kotha, Harika Akula, Vijaya Sarathi Reddy Obulam

Effect of foliar fertilization with zinc and manganese sulfate on yield, dry matter accumulation, and zinc and manganese contents in leaf and seed of chickpea (Cicer arietinum)

Soheil Kobraee

Antibacterial activity of an endophytic fungus Lasiodiplodia pseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum Linn.

Taufiq M.M.J., Darah I.

The effect of fresh stem juice extract of Costus afer on the cytohistomorphology of the kidney in aspirin-treated Wistar rats

Idorenyin U Umoh, AN Aquaisua, NM Udo

Enhancement of pigment production potential of Serratia marcescens (GBB151) through mutation and random amplified polymorphic deoxyribonucleic acid analysis of its mutants

Cecilia Nireti Fakorede, Babamotemi Oluwasola Itakorode, Olu Odeyemi, Gbolahan Babalola

Time-kill assay and post-antibiotic effect of acetone extract from the stem bark of Canarium odontophyllum against Methicillin-resistant Staphylococcus aureus Mu50 strain

Dayang Fredalina Basri, Nur Azmina Yahya, Nur Amira Mohd Shamsuddin

In vitro biological activity of the Croton blanchetianus (Baill) essential oil against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

Onaldo Guedes Rodrigues, Brunna Rodrigues Muniz Falcão, Beatriz Correa Barbosa, Andreia Vieira Pereira, Vitoria Viviane Ferreira de Aquino

Characterization of indole-3-acetic acid in cambial zone tissue by ultra-performance liquid chromatography-tandem mass spectrometry

Renan Marcel Bonilha Dezena, Renata Mauri, Fabio Alessandro Proença Barros, Paulo César Pires Rosa

Polyphenols and triterpenes in leaves and extracts from three Nicotiana species

Venelina Popova, Tanya Ivanova, Albena Stoyanova, Violeta Nikolova, Tsveta Hristeva, Margarita Docheva, Nikolay Nikolov, Ivan Iliev

Biodiversity of psychrotrophic microbes and their biotechnological applications

Ajar Nath Yadav, Neelam Yadav, Shashwati Ghosh Sachan, Anil Kumar Saxena

Comparative evaluation of air pollution tolerance of plants from polluted and non-polluted regions of Bengaluru

BT Manjunath, Jayaram Reddy

Low dose and subchronic Aroclor 1254 induced histopathological changes in mice kidney

Jalpa Raja, Shweta Pathak, Rahul Kundu

Phytochemical analysis, antimicrobial and antioxidant activities of Aidia borneensis leaf extracts

Zulhamizan Awang-Jamil, Aida Maryam Basri, Norhayati Ahmad, Hussein Taha

Weeds in Gwalior and nearby regions and their medicinal properties: A case study

Sapna Kondalkar, Avinash Kondalkar, Vijay Kumar, Anupam K Mangal, Om Prakash

Comparative chemical and biological investigations of three Saudi Astragalus species

Mohamed A. Ashour

Phytochemical analysis and antioxidant potential of Ocimum gratissimum Linn (Lamiaceae) commonly consumed in the Republic of Benin

Hinnoutondji Wilfrid Kpètèhoto, Abdou Madjid Olatoundé Amoussa, Roch Christian Johnson, Eustache Enock Meinsan Houéto , Franck Maurille Zinsou Mignanwandé, Hounnankpon Yédomonhan, Frédéric Loko, Honoré Bankolé, Latifou Lagnika

Determination of phenolic content and antioxidant capacity of Launaea resedifolia from Algerian Sahara

Amina Bouguerra, Mohamed Hadjadj, Mesaouda Dekmouche, Zhour Rahmani, Houssine Dendougui

In vitro anti-amylase activity of some Indian dietary spices

Bhosale Hemlata, Gawali Pornima, Kadam Tukaram, Baisthakur Pankaj

Screening and characterization of siderophore producing endophytic bacteria from Cicer arietinum and Pisum sativum plants

Rajat Maheshwari, Namita Bhutani, Pooja Suneja

Vibriocidal activity of leaf and rhizome extracts of Maranta arundinacea L.

Priyadarshani Samal, Priyaranjan Tripathy, Ritarani Das, Santi Lata Sahoo, Chinmay Pradhan, Bijaya Kumar Padhi, Jyoti Ranjan Rout

Root-fungal associations in plants from home gardens of Tripura, Northeast India

Kripamoy Chakraborty, Atithi Debnath, Aparajita Roy Das, Ajay Krishna Saha, Panna Das

Identification of TNF-Þ-308G/A (rsl800629) polymorphism in Bangladeshi population related to type-2 diabetes

Dipa Roy, Md Sk Shahid Ullah, Bristy Basak, Md. Mahmudul Hasan, Maisha Tanzim, Ariful Haque

Genetics characterization of fresh water clam Spathopsis rubens using ISSR-PCR markers

Gihan Mahmoud Elkhodary, Amal Zaki Ghoneim

Genome-wide analysis of various paralogs of aminopeptidase N (APN) gene in Anopheles gambiae (Diptera: Culicidae)

Renu Jakhar, Ritu Gill, Surender Kumar Gakhar

Factors affecting the chitinase activity of Trichoderma asperellum isolated from agriculture field soils

Ndiogou Gueye, G Kranthi Kumar, Malick Ndiaye, S Y Dienaba Sall, Mame Arama Fall Ndiaye, Tahir A Diop, M Raghu Ram

Kinetic and thermodynamic parameters of four acid phosphatases from edible seeds of oleaginous cucurbit Lagenaria siceraria (Molina) Standl.

Djary Michel Koffi, Jean Bedel Fagbohoun

Nyctanthes arbor-tristis: Comprehensive review on its pharmacological, antioxidant, and anticancer activities

Smita Parekh, Anjali Soni

Bioaccumulation of heavy metal lead (Pb) in different tissues of brackish water fish Mugil cephalus (Linnaeus, 1758)

Vardi Venkateswarlu, Chenji Venkatrayulu

Partial purification and characterization of lectin from serum of American cockroach, Periplaneta americana

Reymend Encily Martin, Yogita Raosaheb Channe

Purification and characterization of extracellular alkaline protease from Streptomyces sp. LCJ12A isolated from Pichavaram mangroves

M. Parthasarathy, J. Joel Gnanadoss

Improvement of germination rate and in vitro multiplication of Lavandula angustifolia

Chaimae Slimani, Hakima Sqalli, Chaimae Rais, Squalli Wafae, Abderrahim Lazraq, Lahsen El Ghadraoui, Saadia Belmalha, Ghizlane Echchgadda

A simple and efficient method for removal of phenolic contaminants in wastewater using polyacrylamide entrapped mushroom tyrosinase

Anupama Sharma Avasthi, Sabari Ghosal

In vitro anthelmintic activity of methanol extracts and fractions of two amphilophium species against Eisenia Fetida

Diana Bazana, Ever Lopezb, Andrea Caceresa, Rosa Degenb, Nelson Alvarengaa

Screening and evaluation of PGPR strains having multiple PGP traits from hilly terrain

Teg Bahadur Singh, Vikram Sahai, Akbar Ali, Mrinalini Prasad, Arti Yadav, Preksha Shrivastav, Deepika Goyal, Prem Kumar Dantu

The phytochemical and chemotaxonomic study of Salvia spp. growing in Ukraine

Oleh Koshovyi, Ain Raal, Alla Kovaleva, Mykhailo Myha, Tetyana Ilina, Natalia Borodina, Andrey Komissarenko

Optimization of physical parameters for the growth and lipid production in Nannochloropsis gaditana (Lubian, 1982)

Shyni MarKose, Ajan Chellappan, Praba Thangamani, Subilal George, Selvaraj Thangaswamy, Citarasu Thavasimuthu, Michaelbabu Mariavincent

Efficacy of antibiotic sensitivity and antimicrobial activity of Streptomyces cinereoruber RSA-14 isolated from rhizosphere soil of Alternanthera sessilis (L.) R. Br. ex DC

Sreenivasa Nayaka, Halaswamy Hiremath, Bidhayak Chakraborty, Pallavi Sathyanarayan Swamy, Dhanyakumara Shivapoojar Basavarajappa, Shashiraj Kareyallappa Nagaraja, Muthuraj Rudrappa, Meghashyama Prabhakara Bhat, Dattatraya Airodagi, Meenakshi Shivanandagouda Murigennavar

Taxonomic validation of Areolate grouper, Epinephelus areolatus (Perciformes: Serranidae) along the Nizampatnam coast, India

Chatla Darwin, Padmavathi Pamulapati, Gatreddi Srinu

Purification and characterization of an iron-activated alkaline phosphatase produced by Rhizopus microsporus var. microsporus under submerged fermentation using rye flour

Pedro Henrique de Oliveira Ornela, Luis Henrique Souza Guimarães

Comparison of diagnostic performance between serological tests (Scrub IgM-ELISA) and Scrub-PCR for scrub typhus fever in an Indian tertiary care teaching hospital

Anil Gatika, Subrat Kumar Swain, Jatindra Nath Mohanty, Rachita Sarangi

Determination of the activity and kinetic parameters of polyphenol oxidase enzyme in crude extracts of some Jordanian plants

Omar Mohammad Atrooz, Nooh Khair AlKhamaisa, Ibrahim M. AlRawashdeh

Physicochemical, phytochemical, and GC–MS analysis of leaf and fruit of Pouteria campechiana (Kunth) Baehni

Akshata Pai, Chandrakala Shenoy

In vitro antioxidant activity of Lactobacillus plantarum against hydrogen peroxide-induced neuronal damage on PC12 cells

Shani Kunjamma John, Vani Chandrapragasam

Identification and in-silico characterization of differentially expressed salt-induced proteins in the leaves of mangrove grass Myriostachya wightiana

Mathikani Kiran Kumar, B V Sandeep, Pola Sudhakar

Distribution and species listing of wild macrofungi in Sitio Canding, Barangay Maasin, San Clemente, Tarlac Province, Philippines

Rich Milton Dulay, Jose Santos Carandang VI, Sofronio Kalaw, Renato Reyes

Development and validation of multiplex polymerase chain reaction assay for concomitant detection of genus Staphylococcus and clinically relevant methicillin resistance determinants

Nimita Venugopal, Feroze Ganaie, Susweta Mitra, Rituparna Tewari, Tushar K. Dey, Rakshith Ojha, Rajeswari Shome, Bibek R. Shome

Distribution and diversity of endophytic fungi associated with three medicinal tree species from Eturnagaram Wildlife Sanctuary, TS, India

Bhavani Vemireddy, Abhinesh Madasi, Aruna Ajmeera, Krishna Reddy Vanteru

Interactive potential of Pseudomonas species with plants

Suhana Shaikh,, Nutan Yadav, Anoop R. Markande,

Screening of in vitro sun protection factor of some medicinal plant extracts by ultraviolet spectroscopy method

Manisha Pralhad Sutar, Sanjay Ravindra Chaudhari

Molecular identification and optimization of cultural conditions for mycelial biomass production of wild strain of Chlorophyllum molybdites (G.Mey) Massee from the Philippines

Benjie L. Garcia, Jerwin R. Undan, Rich Milton R. Dulay, Sofronio P. Kalaw, Renato G. Reyes

Effects of culture media and physical factors on the mycelial growth of the three wild strains of Volvariella volvacea from Ecuador

Marcelino D. Abon, Rich Milton R. Dulay, Sofronio P. Kalaw, Maria Eugenia Romero-Roman, Lenin Pedro Arana-Vera, Walter Oswaldo Reyes-Borja, Renato G. Reyes

Identification of highest L-Methioninase enzyme producers among soil microbial isolates, with potential antioxidant and anticancer properties

D. Kavya, Varalakshmi Kilingar Nadumane

African plum (Dacryodes edulis [G. Don] H.J. Lam) fruit development indexes clearly defined and phenophases correlated with temperature in the tropics

Salamatou Mpemboura Nsangou, Libert Brice Tonfack, Didier Mbeguie-A-Mbeguie, Carine Temegne Nono, Godswill Ntsomboh-Ntsefong, Emmanuel Youmbi

Comparative morphological, histological, and RAPD analysis of Columba livia domestica strains

Dalia A. Sabry, Yosra A. Fouda

An effective and eco-friendly technique for control of post-harvest fungal pathogens of orange (Citrus sinensis) isolated from the distribution chain of Delhi NCR

Gayatri Krishna, Geethu Gopinath, Anupama Sharma Avasthi

Amylase production by Aspergillus niger in submerged cultivation using cassava

Muralikandhan Kamaraj, Dhanasekaran Subramaniam

Evaluation of thermogravimetric analysis as a rapid tool for the detection of rhizobacteria biostimulants used in precision agriculture

Eugene Carmichael, Juluri R. Rao

Multidrug-resistant Candida auris: A global challenge

Hardeep Kaur, Khushbu Wadhwa, Kusum Jain, Anamika Yadav

Evolution of matK Gene among the Elite Tea Clones (Camellia sinensis) Revealed by Nucleotide Substitution within the Consensus Region

Reha Labar, Pallab Kar, Prosenjit Biswas, Arnab Sen, Malay Bhattacharya

In-vitro activity of ethanolic extract of Lentinus strigosus mycelia in N2 wild strain Caenorhabditis elegans – An animal model for obesity and its chemical composition

Benditha D. Babac, Rich Milton R. Dulay, Reign Arwen S. Calpito, Maricris A. Domingo, Mary Grace M. Macamos, Andrea R. Mangabat, Neil Zhyra L. Zoberiaga

The comparative antimicrobial and anticancer of chemical extract from in vitro and in vivo Peperomia pellucida plantlet

Lydia Teoh, Nareshwaran Gnanasegaran, Ahmad Faris Mohd Adnan, Rosna Mat Taha

Floral induction pathways: Decision making and determination in plants to flower-A comprehensive review

Latif Ahmad Peer, Mohd Yaqub Bhat, Nusrat Ahmad, Bilal Ahmad Mir

Plasticity of tandem repeats in expressed sequence tags of angiospermic and non-angiospermic species: Insight into cladistic, phenetic, and elementary explorations

Shamshad Ul Haq, Prerna Dhingra, Meenakshi Sharma, S. L. Kothari, Sumita Kachhwaha

Effects of light on cell growth, chlorophyll, and carotenoid contents of Chlorella sorokiniana and Ankistrodesmus falcatus in poultry dropping medium

Jane Chizie Ogbonna, Nkechinyere Onyekwere Nweze, Christiana Nwakego Ogbonna

Some roadside medicinal weeds as bio-indicator of air pollution in Kolkata

Pranabesh Ghosh, Sirshendu Chatterjee, Suradipa Choudhury, Tanusree Sarkar, Ahana Sarkar, Susmita Poddar

Microbial biotechnology for bio-prospecting of microbial bioactive compounds and secondary metabolites

Ajar Nath Yadav

Evaluation of immunomodulatory activity of Immusante® in zebrafish

Anna Venkata Agraz, Mohamed Rafiq, Bhavimani Guru, Madan MN, Suryakanth Dattatreya Anturlikar, Azeemuddin MM, S. N. Manjula

Bioprospecting of endophytic bacteria from the Indian Himalayas and their role in plant growth promotion of maize (Zea mays L.)

Kusam Lata Rana, Divjot Kour, Tanvir Kaur, Rubee Devi, Ashok Yadav, Ajar Nath Yadav

Effect of perforation size and substrate bag fruiting position on the morphology of fruiting bodies and clusters in Pleurotus ostreatus (Jacq.) P. Kumm

Iryna Bandura, Omoanghe S. Isikhuemhen, Alina Kulik, Marina Serdiuk, Olena Sucharenko, Valentina Jukova, Viktoriia Koliadenko, Nona Gaprindashvili

Optimization of culture conditions for mycelial growth and fruiting body production of naturally-occurring Philippine mushroom Lentinus swartzii Berk

Rich Milton R. Dulay, Esperanza C. Cabrera, Sofronio P. Kalaw, Renato G. Reyes

Modulation of photosynthesis, nitrogen fixing ability, and yield attributes of Chickpea (Cicer arietinum L.) to interactive effect NPK fertilizers and municipal wastewater irrigation

Hamid Iqbal Tak

Modification of the time of incubation in colorimetric method for accurate determination of the total antioxidants capacity using 2,2-diphenyl-1-picrylhydrazyl stable free radical

Abhipsa Bal, Samar Gourav Pati, Falguni Panda, Biswaranjan Paital

Differences in antibiotic resistance profiles of methicillin-susceptible and –resistant Staphylococcus aureus isolated from the teaching hospital in Kuala Lumpur, Malaysia

Asif Sukri, Muhammad Nur Farhan Saat, Nor Afnizan Mohd Yusof, Noraziah Mohamad Zin, Abdul Rahim Abdul Rachman

Role of lacto-fermentation in reduction of antinutrients in plant-based foods

Mehak Manzoor, Deepti Singh, Gajender Kumar Aseri, Jagdip Singh Sohal, Shilpa Vij, Deepansh Sharma

Antimicrobial activity of the lichens Parmotrema andium and Dirinaria applanata

Bijayananda Sahoo, Satyabrata Dash, Sabyasachy Parida, Jayanta Kumar Sahu, Biswajit Rath

Micro-morphological and anatomical response of groundnut (Arachis hypogaea L.) cultivars to ground-level ozone

Indra Jeet Chaudhary, Dheeraj Rathore

Biodiversity and bioprospecting of extremophilic microbiomes for agro-environmental sustainability

Ajar Nath Yadav

Acute toxic effects of chlorpyrifos and indoxacarb on dorsal pars medialis of telencephalon and swimming behavior of a catfish, Heteropneustes fossilis

Ajeet Kr. Mishra, Anita Gopesh, K. P. Singh

Role of medicinal plants in the treatment of eumycetoma: A review

Shashank M. Patil, S. Jagadeep Chandra, M. K. Jayanthi, Prithvi S. Shirahatti, Ramith Ramu

Analysis of the chemical composition (GC–MS) of Lantana camara (Verbenaceae) essential oil and its insecticidal effect on the post-embryonic development of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae)

Rahul Maddheshiya, Deep Mala Sahu, Krishna Pal Singh

Identification and bioactivities of endophytic fungi from Lagenandra toxicaria Dalz. and Kaempferia rotunda L.

Praveen Krishnakumar, Mable Varghese, Maria Grace Joe, Asha Rajagopal, Leyon Varghese

The effect of Staphylococcus epidermidis cells on Pseudomonas aeruginosa-associated virulence factors

Suhaga Dohare, Devendra Singh, Deepmala Sharma, Vishnu Agarwal

Beneficial effects of soaking and germination on nutritional quality and bioactive compounds of biofortified wheat derivatives

Meena Verma, Vinod Kumar, Imran Sheikh, Punesh Sangwan, Roop Singh Bora, Ajar Nath Yadav, Harcharan Singh Dhaliwal

Characterization of Calotropis procera root peroxidase and its potential to mediate remediation of phenolic pollutant from petroleum refinery effluent

Enoch Banbilbwa Joel, Simon Gabriel Mafulul, Ezra Adams Jeremiah, Adepeju Aberuagba, Raphael Idowu Adeoye, Lazarus Joseph Goje, Adedoyin Igunnu, Sylvia Omonirume Malomo

Applications of bacterial endophytes and their advanced identification methodologies

R. Renugadevi, M. P. Ayyappadas, V. Subha Priya, M. Flory Shobana, K. Vivekanandhan

Morphological status of multipolar neurons in the lateral cortical area of the telencephalon of a catfish, Clarias batrachus: A Golgi study

Banalata Mohanty, Anil Kumar Ojha

Prevalence of malaria and typhoid fever and coinfection in all febrile patients attending at tertiary care hospital in Vadodara

Sangita Devubhai Vasava, Sucheta Jitendra Lakhani, Jitendra Devjibhai Lakhani

Doehlert Matrix-assisted optimization of Salmonella typhi Vi polysaccharide purification parameters

Garisala Mallikarjunara Rao, Sathish Thadikamala, Maram Chinna Eswaraiah

Purification, characterization of α-galactosidase from a novel Bacillus megaterium VHM1, and its applications in the food industry

Aravind Gouda G. Patil, Naganagouda V. Kote, A. C. Manjula, T. Vishwanatha

Diversity and antimicrobial potential of endophytic fungi from aromatic plants of Bhadra Wildlife Sanctuary, Western Ghats, Karnataka

Rajeshwari Jagadish, Srinivas Chowdappa

Molecular docking of active compounds from Phoenix pusilla root extract against antidiabetic and anti-inflammatory drug targets

Vijayabharathi Saravanan, Anuradha Venkatraman, Habeeb Shaik Mohideen

Rice crop loss due to major pathogens and the potential of endophytic microbes for their control and management

Shubhransu Nayak, Soma Samanta, Chandan Sengupta, Soumya Sephalika Swain

DNA barcode of matK combined with ITS effectively distinguishes the medicinal plant Stephania brachyandra Diels collected in Laocai, Vietnam

Nhan Thi Thanh Pham, Dung Phuong Le, Khanh Thi Ngoc Pham,, Xaykham Thipphavong,, Mau Hoang Chu

Molecular identification of endophytic fungi associated with Coleus forskohlii (Willd.) Briq.

Grace Leena Crasta,, Koteshwar Anandrao Raveesha,

Augmentative role of Piriformospora indica fungus and plant growth promoting bacteria in mitigating salinity stress in Trigonella foenum-graecum

Sanskriti Bisht, Shatrupa Singh, Madhulika Singh,, Jai Gopal Sharma

Phytomicrobiomes for agro-environmental sustainability

Ajar Nath Yadav

Analytical study on hexavalent chromium accumulation in plant parts of Pongamia pinnata (L.) Pierre and remediation of contaminated soil

Pratyush Kumar Das, Bidyut Prava Das, Patitapaban Dash

Bacterial endophytes from halophyte black saxaul (Haloxylon aphyllum Minkw.) and their plant growth-promoting properties

Vyacheslav Shurigin,, Begali Alikulov, Kakhramon Davranov, Zafar Ismailov

The ITS2 DNA sequence analysis in six species of barbin fishes with phylogenetic insights

Sandip Choudhury, Briyanka Kashyap, Karabi Dutta

Mushroom fruiting body yield and morphological characteristics from different strains of Pleurotus eryngii

Iryna Bandura, Omoanghe S. Isikhuemhen, Alina Kulik, Nina Bisko, Maryna Serduik, Volodymyr Khareba, Olena Khareba, Iryna Ivanova, Olesya Priss, Oleksandr Tsyz, Serhii Makohon, Serhii Chausov

Partial identification of a peptide from Lacticaseibacillus casei using MALDI TOF and it’s cytotoxic activity against MDA-MB-231 cell line

Jannatul Firdous Siddique, Mohanasrinivasan Vaithilingam

Synergistic effect of Hydnophytum formicarum tuber and Vatica diospyroides Symington cotyledon extracts with ampicillin on pathogenic bacteria

Kanokrat Keawchai, Parinuch Chumkaew, Patima Permpoonpattana, Theera Srisawat

Allelopathic potential of Annona muricata (L.) on physiological and biochemical changes of Vigna radiata (L.) and Eleusine coracana (L.) Gaertn.

Edwina Kannan, Leela Palayian

Phytochemical, pharmacological, and pharmacognostic overview of Merremia tridentata (L.) Hallier

Paras Sharma, Gaurav Gupta

Genetic polymorphism of soybean genotypes with contrasting levels of phosphatidylcholine, protein, and lipoxygenase-2

Aseem Kumar Anshu,, Trupti Tayalkar, Anita Rani, Vineet Kumar, Hamendra Singh Parmar

Suppression of the RAGE gene expression in RAW 264.7 murine leukemia cell line by ethyl acetate extract of Mikania micrantha (L.) Kunth.

Alex Zohmachhuana, Malsawmdawngliana Tlaisun, Vabeiryureilai Mathipi, Lalrinzuali Khawlhring, Joyce Sudandara Priya

Dietary product based on sea urchin caviar and Sardinops melanostictus fat

Mouhamad Alrajab, Lidiya Vasilevna Shulgina

Characterization and comparative assessment of bactericidal activity of carbon nanodots (CDs) and nanoparticles (CNPs) prepared from soot's of clarified butter and mustard oil, respectively

Vikas Pahal, Pankaj Kumar, Rahul Kumar, Parveen Kumar, Vinod Kumar

Inducing the growth and flowering of caraway (Carum carvi L.) plant

Hanan M. H. Ali, Dalia Abdel Halim M. Sallam

Isolation and identification of potent tributyltin chloride utilizing Pseudomonas sp. strain VBAK from Vishakhapatnam shipping harbor sediments

Battu Anand Kumar, Triveni Gummadi, Bhargava Ram Gundluri, Chanda Vikrant Berde, Veera Bramhachari Pallaval

Characterization and expression of phenylalanine hydroxylase in rabbits under different feeding conditions of faba bean (Vicia faba L.)

Hanan I. Sayed Ahmed, Ghada Shebl, Mohamed Hamza, Asraf Haider

Quantification of phytochemicals and in vitro antioxidant activities from various parts of Euphorbia neriifolia Linn.

Priya Chaudhary, Pracheta Janmeda

Effect of extraction methods and temperature preservation on total anthocyanins compounds of Peristrophe bivalvis L. Merr leaf

Nguyen Minh Thuy, Dao Huynh Ngoc Han, Vo Quang Minh, Ngo Van Tai

Molecular detection of Haemophilus influenzae isolated from eye swabs of patients with conjunctivitis in Hilla Province, Iraq

Jundi Alak Mahdi Al-Buhilal, Abdulkadir Kareem Rhumaid, Alyaa Mohammed H. Al-Tabtabai, Niran Kadhim F. AL-Rubaey

The propensity of selected Indian plant extracts for polyphenolics, antioxidant, and inhibition of Pseudomonas aeruginosa biofilms through type-3 secretion system

M. G. Avinash, Farhan Zameer,, Shubha Gopal

Purification and characterization of a polygalacturonase from the xylophagous insect Oncideres albomarginata chamela (Coleoptera: Cerambycidae)

Alicia Lara-Márquez, Nayeli Soria-Calderón, Maria Guadalupe Villa-Rivera, Everardo López-Romero, Nancy Calderón-Cortés

Investigating Musa paradisiaca (Var. Nanjangud rasa bale) pseudostem in preventing hyperglycemia along with improvement of diabetic complications

Ramith Ramu,, Prithvi S Shirahatti, Deepika TH, Shrisha Naik Bajpe, Navya Sreepathi,, Shashank M Patil, Nagendra Prasad MN

Isolation and partial characterization of amylase produced by fungal isolates from the agro-industrial waste source

Soumya Nandi, Twinkle Bose, Susmita Mahato, Somashree Chatterjee, Annalakshmi Chatterjee

Virulence of native entomopathogenic nematodes against major lepidopteran insect species of tomato (Solanum lycopersicum L.)

Neelam Thakur, Preety Tomar, Simranjeet Kaur, Poonam Kumari

Molecular diversity and phylogenetic analysis of exopolymer producing fungal strains isolated from Krishna river mangrove sediments

Annavarapu Mohana Ventaka Naga Prathyusha, Venkateswarulu Nagam, Chanda Vikrant Berde, Siva Saikiran Badithala, Muni Ramanna Gari Subhosh Chandra, Thummala Chandra Sekhar, Veera Bramhachari Pallaval

Microbes for Agricultural and Environmental Sustainability

Ajar Nath Yadav, Divjot Kour, Ahmed M. Abdel-Azeem, Murat Dikilitas, Abd El-Latif Hesham, Amrik Singh Ahluwalia

Characterization of tannery effluents by analyzing the recalcitrant organic pollutants and phytotoxicity assay

Sandeep Kumar, Ashutosh Yadav, Annapurna Maurya,,Shalini G. Pratap, Pramod Kumar Singh, Abhay Raj,

In silico studies of viral protein inhibitors of Marburg virus using phytochemicals from Andrographis paniculata

Ragupathy Hariprasath, Chandrasekar Akashpriya, Vasantha Veerappa Lakshmaiah, Nagella Praveen

Cowpea (Vigna unguiculata L. Walp.): A choice crop for sustainability during the climate change periods

Tewodros Ayalew, Tarekegn Yoseph

Woodfordia fruticosa (Linn.) Kurz’s fungal endophyte Mucor souzae’s secondary metabolites, kaempferol and quercetin, bestow biological activities

Kavyashree Doreswamy, Priyanka Shenoy, Sneha Bhaskar, Ramachandra K. Kini, Shailasree Sekhar

Characterization of the crude extract of Portulaca oleracea and the determination of the polyphenol oxidase kinetics in the presence of Cu and Zn

Omar Mohammad Atrooz, Shada Zaher Al-Maitah

Assessing DNA barcodes species discriminating ability and phylogenetic relation within Embelia species

Shrisha Naik Bajpe,, K. M. Marulasiddaswamy, Ramith Ramu, Abhijeeth S. Badiger, Maruthi Katenahally Rudrappa, Ramachandra Kukkundoor Kini

Molecular characterization and antibacterial properties of endophytic fungi Lasidiplodia theobromae in Lobelia nicotianifolia Roth ex Schult. of central Western Ghats of Karnataka, India

Krishnappa Vinu, Maddappa Krishnappa, Venkatarangaiah Krishna

Seasonal effect on the diversity of soil fungi and screening for arsenic tolerance and their remediation

Dheeraj Pandey, Harbans Kaur Kehri, Ifra Zoomi, Shweta Chaturvedi, Kanhaiya Lal Chaudhary

Enhancing antibacterial properties of bacteriocins using combination therapy

Poonam Sharma, Meena Yadav

Phenotypic variation and genetic divergence studies in Cameroonian potato (Solanum tuberosum L.) genotypes

Mariette Anoumaa, Eric Bertrand Kouam, Gabriel Kanmegne, Yanick Borel Kamga, Hervé Djomo Sime, Lisette Laure Chimi Nkombo, Théophile Fonkou

Phosphate-Solubilizing Microorganisms for Agricultural Sustainability

Ajar Nath Yadav

Additive and antagonistic effects of selected polyphenols on biochemical indices of isoproterenol-induced toxicity in Wistar rats

Grace Jadesola Oyinbogbola, Mary Tolulope Olaleye, Aanuoluwapo Ruth Adetuyi, Ibrahim Olabayode Saliu, Afolabi Clement Akinmoladun

Identification, production, and purification of a novel lipase from Bacillus safensis

Krishna Patel, Samir Parikh

Evaluation of plant growth-promoting activities of endophytic bacteria of Musa acuminata and their characterization

Shilpi Singh, Kamlesh Choure, Piyush Kant Rai, Sourabh Singh Gour, Vivek Kumar Agnihotri

Evolution of machine learning in biosciences: A bibliometric network analysis

Akkinepally Vanaja,, Venkata Rajesh Yella

Isolation and characterization of starch degrading bacteria from disparate soil samples

Vidhyutha Srivathsan, Mahima Bhandari, Priya Swaminathan

Antimicrobial, anticancer, and antioxidant potential of two dominant macro-lichen Dirinaria aegialita and Parmotrema praesorediosum collected from Similipal Biosphere Reserve of Odisha, India

Srimay Pradhan, Dalip Kumar Upreti, Rajesh Kumar Meher, Kunja Bihari Satapathy

An epidemiological outbreak of scrub typhus caused by Orientia tsutsugamushi – A comprehensive review

Subhasmita Panda, Subrat Kumar Swain, Rachita Sarangi

Investigation on the antifungal activity of Aspergillus giganteus in different culture conditions

S. Karthiga, R. Ramya, K. Ramya, S. Jothinayaki, D. Kavitha

Accumulation of cadmium in maize roots inoculated with root organ culture of Rhizophagus irregularis improving cadmium tolerance through activation of antioxidative defense enzymes

Rajamony VinuRadha, Karunanandham Kumutha, Asish Kanagaraj Binodh

Comprehensive reviews on phenolic compounds from Phaeophyceae as potential therapeutic agent

V. Maheswari, P. Azhagu Saravana Babu

Endophytic bacterial metagenomics and phosphate solubilization activities in an endemic legume Humboldtia brunonis Wall.

Ganesh V. Shendye, N. Thamizhseran

Identification of phytocompounds from Paris polyphylla Smith as potential inhibitors against two breast cancer receptors (ERÞ and EGFR tyrosine kinase) through chromatographic and In silico approaches

Debmalya Das Gupta, Saurov Mahanta, Sasti Gopal Das, Sanjib Kumar Das, Dipayan Paul, Hui Tag, Pallabi Kalita Hui

In silico modeling, docking of ThPON1-like protein, and in vitro validation of pesticide tolerance in Trichoderma harzianum

Archana Kumari, Krishna Sundari Sattiraju

Comparative analysis of Physico-chemical properties and fatty acid composition of linseed (Linum usitatissimum L.) oils of Indian accessions

Nagabhushanam Beema, Nagaraju Mukkamula, Srinivas Mothuku, Ravinder Thumu, Thirupathi Azmeera, Kiran Kumar Biman

In vitro evaluation of the antibacterial potential of flavonoid glycosides from Glinus oppositifolius (L.) Aug. DC.

K. Suresh Kannan, D. Kandavel, P. Rajalakshmi, P. Maheswari

Elevating algal biomass generation toward sustainable utilization for high value added biomolecules generations

Kamalendu De, Shrestha Debnath, Dipankar Ghosh

Antimycobacterial and antibiofilm activity of garlic essential oil using vapor phase techniques

Ashirbad Sarangi, Bhabani Shankar Das, Sunil Swick Rout, Ambika Sahoo, Sidhartha Giri, Debapriya Bhattacharya

Development of Ready-to-Eat functional food prepared from seeded banana Bhimkol (Musa balbisiana Colla) and Black rice (Oryza sativa L.)

Himakshi Baishya, Ranjita Goswami, Lakhya Jyoti Gogoi

Multitargeted molecular docking study of phytochemicals on hepatocellular carcinoma

Vikas Jha, Anjali Bhosale, Prakruti Kapadia, Agraj Bhargava, Arpita Marick, Zahra Charania, Omkar Parulekar, Mafiz Shaikh, Bhakti Madaye

Growing rice (Oryza sativa L.) in the northern Gangetic plains of India. How suitable are they? A GIS-based multi-criteria analysis

Sambit Shome, Hina Upadhyay

Degradation and detoxification of monocrotophos using bacterial consortium

Priyanka Harenkumar Jokhakar, Pravin R Dudhagara

Occurrence of two endophytic associative nitrogen-fixing Caulobacter spp., from three non-nodulating endemic legumes based on nifH gene analysis

N. Thamizhseran, Ganesh V. Shendye

A comprehensive review on global research trends on Aerides genus with reference to Aerides odorata species

Vivek Kumar Paraste, Surendra Sarsaiya,, Umesh C. Mishra, Pragya Sourabh

Records of wild mushrooms in the Philippines: A review

Rich Milton R. Dulay, Joshua N. Batangan, Sofronio P. Kalaw, Angeles M. De Leon, Esperanza C. Cabrera, Kenichiro Kimura, Fumio Eguchi, Renato G. Reyes

Transaminases activity in the hemolymph: Biomarkers determining the thermal stress in the new bivoltine lines of Bombyx mori

J. Prashanth, H. B. Manjunatha

Impact of different levels of iron fertilizer on growth and yield physiology of Kodo millet under rainfed conditions – An overview

R. Samundeswari, N. Jeyapandiyan, M. Anitha, J. Patricia Kalaiarasi, R. Susan Poonguzhali, C. Jayapradha, S. Rathikannu, K. Udhaya Kumar

Field treatment of three wheat varieties with Trichoderma harzianum bioagent to control Anguina tritici

Nawres Abdulelah Sadeq Alkuwaiti, Ammar Amjad Aish, Saad Tareq Abdulmalak, Tariq A. Kareem, Mohammed Mahmood Sulaiman

Elemental, nutritional, and phytochemical profiling and antioxidant activity of Cordia obliqua Willd. (Clammy Cherry): An important underutilized forest tree of East India

Mamta Naik#,, Shashikanta Behera#,,, Sadhni Induar, Swaraj K. Babu, Pradeep K. Naik

Endophytic nitrogen-fixing bacteria: Untapped treasurer for agricultural sustainability

Kusam Lata Rana, Divjot Kour, Tanvir Kaur, Rajeshwari Negi, Rubee Devi, Neelam Yadav, Pankaj Kumar Rai, Sangram Singh, Ashutosh Kumar Rai, Ashok Yadav, R. Z. Sayyed, Ajar Nath Yadav

Rhizospheres of Rubus ellipticus and Ageratina riparia from Meghalaya exhibit Actinomycetota that promote plant growth

Debulman Syiemiong, Dhruva Kumar Jha, Samrat Adhikari, Dapkupar Mylliemngap, Richborn Kharbuki, Dominic Lyngdoh, Joel Paul Warlarpih, Neha Paul, Kevin Matthew Lamare, Chalcedony Wahlang, Rangehbok Lyngkhoi

Effect of salicylic acid, jasmonic acid, and a combination of both on andrographolide production in cell suspension cultures of Andrographis paniculata (Burm.f.) Nees

Nihal Ahmed, N. Praveen

Antimicrobial peptide coding gene of thermophilic bacteria isolated from crater hot spring in mountains around West Java

Emma Rachmawati, Sinta Asarina, Gabriel Bagus Kennardi, Ratu Safitri, Toto Subroto, Ani Melani Maskoen

Endophytic Fungi as Emerging Bioresources for Bioactive Compounds for Sustainable Development

Divjot Kour, Neelam Yadav, Ajar Nath Yadav

Pseudomonas aeruginosa biofilm and their molecular escape strategies

M. G. Avinash, S. Aishwarya, Farhan Zameer, Shubha Gopal

Effects of gut bacteria and their amyloids on mental health and neurodegeneration in Parkinson’s disease

Kush K Mehta, Radhika Bhat, Anoop R Markande

Neutrophil gelatinase-associated lipocalin a proinflammatory polypeptide necessary for host cell survival in bacterial infection

Nichita Yadav Aare, Pawan Kumar Anoor, Swathi Raju M, N. Srinivas Naik, Sandeepta Burgula

Characterization of indole-3-acetic acid biosynthesis and stability from Micrococcus luteus

Patcha Boonmahome, Wiyada Mongkolthanaruk

Total phenolic, flavonoid contents, and antioxidant activity of three selected Portulaca grandiflora mutants in MV8 generation as a result of recurrent irradiation technique

Waras Nurcholis,, Syarifah Iis Aisyah, Regina Agritena Mayrischa Saraswati, Yoshua Shandy Yudha

Evaluating DNA barcoding using five loci (matK, ITS, trnH- psbA, rpoB, and rbcL) for species identification and phylogenetic analysis of Capsicum frutescens

Meetali Chinnkar, Pratima Jadhav

Effect of bacteriophages and chamber bitter (Phyllanthus amarus) in combination on Vibrio parahaemolyticus

Truong Thi Bich Van, Do Tan Khang, Le Viet Dung, Le Hoang Bao Ngoc, Nguyen Pham Anh Thi, Tran Thanh Men, Vo Ngoc Tram Anh, Nguyen Kim Thoa

Enhanced probiotic attributes of lactobacilli in honey supplemented media

Jinal Bhola, Manasi Gokhale, Suraj Prajapati, Rama Bhadekar

Chemical profiling, in vitro antibacterial, and cytotoxic properties of Elytranthe parasitica (L.) Danser – A hemiparasitic Indian mistletoe

Keragodu Paramesh Sharath, Raja Naika

Thidiazuron-mediated and genotype-independent regeneration system for tomato (Solanum lycopersicum L.)

Arshlyonel C. Velda, Sherry Anne G. Rodriguez, Michelle Lyka V. Descalsota, Olivia P. Damasco

Evaluation of the phytochemical constituents and tumor reduction potentials of Tragia involucrata Linn.

Madhuri Menon, Leyon Varghese

The de novo genome assembly (nuclear, chloroplast, and mitochondria) of ornamental plant pygmy date palm Phoenix roebelenii

Navajeet Chakravartty, Nageswara Rao Reddy Neelapu

Influence of different sources of fertilizers and weed control treatments on growth, phenology, and yield of baby corn (Zea mays L.) in the semi-arid region of India

Mustapha Na-Allah Sale, Gurpreet Singh, Sandeep Menon, Jayanti Yomso

Identification of matrix metalloproteinases inhibitors from methanol extract of Peltophorum pterocarpum leaves

Bhimrao V. Jaiwal, Faiyaz K. Shaikh, Sarwan W. Bradosty, Ajit B. Patil, Raghavendra L. S. Hallur

An exploration of the phylogeny and phylogeographic relationships of the subfamily Salacioideae

Shrisha Naik Bajpe, Kuppuru Mallikarjunaiah Marulasiddaswamy, Manu G, Abhijeeth S Badiger, Ramith Ramu, Maruthi Katenahally Rudrappa, Kukkundoor Ramachandra Kini

Antimicrobial potential of Indian cooperative spider, Stegodyphus sarasinorum, and Golden orb spider, Nephila pilipes hemolymph against pathogenic bacteria

Dhanapal Sangavi, Padur Sankaranarayanan Anisha, Douglas J. H. Shyu, Parthasarathy Thiruchenthil Nathan

Timing of phenological events and fruit ripening in Ficus palmata forssk. In the Mid Himalayan Region

Jyotsna Tamta, Ashish Tewari, Krishna Kumar Tamta, Shruti Shah

Elastase and COVID-19 relationship, and potential natural resource as elastase inhibitors: A comprehensive review

Neneng Siti Silfi Ambarwati, Yesi Desmiaty, Islamudin Ahmad, Erwin Samsul, Adam M. Ramadhan

Effects of herbicide on various life forms with special reference to the paddy fields in the Eastern Belts of India

Tripti Kanda, Rupanshee Srivastava, Sadhana Yadav, Nidhi Singh, Rajesh Prajapati, Shivam Yadav, Rajeev Mishra, Neelam Atri

Comparative analysis of purified anti-leukemic L-asparaginase enzyme from Trichoderma spp.

Kuldeep Luhana, Himanshu Bariya

Utilization of agro-industrial wastes as raw materials for the biosynthesis of polyhydroxy butyrate biopolymer from Bacillus paramycoides strain KUMBNGBT-33

G. Nandish, S. Akarsh, D. Manjunatha, H. V. Sowmya, B. Thippeswamy

Dietary Supplementation of Synbiotic Formulation with Phytoactives on Broiler Performance, Relative Ready-to-Cook Weight, Health, Nutrient Digestibility, Gut Health, and Litter Characteristics

Vishwanath G Bhagwat, Santoshkumar V G Tattimani, Mirza Rizwan Baig

Beneficial microorganisms for healthy soils, healthy plants and healthy humans

Ajar Nath Yadav, Divjot Kour, Neelam Yadav

In vitro antioxidant and antibacterial potential of biosynthesized yttrium oxide nanoparticles using floral extract of Illicium verum

Karthikeyan Kandasamy, Premkumar Kumpati

Random amplified polymorphic DNA marker molecular and physiological characteristics in tomato plants induced by chemical mutagen

Slameto Slameto, Sigit Soeparjono, Raden Soedrajad, Ketut Anom Wijaya, Usmadi Usmadi, Distiana Wulanjari, Danil Eka Fahrudin

Sensing oxy anodic microbial fuel cell potential of halotolerant protease producer Priestia megaterium BorS17B13 belonging to mangrove biota

Priyanka S. Sawant, Jignasha T. Thumar

Evaluating the anti-inflammatory potential of Mesua ferrea linn. stem bark through network pharmacology approach

Jyothsna Kalyana Sundaram, Manjunatha Hanumanthappa, Shivananada Kandagalla, Sharath Belenahalli Shekarappa, Pavan Gollapalli, Umme Hani

Potential of some phages against Pseudomonas solanacearum causing bacterial wilt in tomato

Ai Nhi Nguyen,, Ngoc Quynh Anh Pham,, Hue Ngoc To,, Nam Vo,, Quang Vinh Tu,, Minh Thien Nguyen,, Duc Hoang Nguyen,, Thi Thuy Tien Le,, Thi Phuong Trang Phan,, Do Tra My Pham,, Anh Hoang Hoang,

DNA barcoding for species identification and phylogenetic investigation employing five genetic markers of Withania coagulans

Neelam Balkrishna Bare, Pratima Sharad Jadhav, Manivel Ponnuchamy

Effect of pre emergence herbicides on weed dynamics and production potential of rice (Oryza sativa) under SRI

G. Bhargavi, A. Sundari, S. Kalaisudarson, G. Usharani

Immune cells at the maternal-fetal interphase: Role in implantation and establishment of tolerance

Anju Kaicker

Isolation of toxic gas-producing bacteria (Desulfovibrio spp.) from shrimp ponds and potential of bacteriophages as biocontrol

Truong Thi Bich Van, Tran Vo Minh Thu

Effect of combined NPK fertilizer on polyphenol contents and antioxidant activity in methanol extract of Curcuma xanthorhiza

Minarni Minarni, Rayandra Asyhar, Amira Amandanisa, Sintya Ainun, Yoshua Shandy Yudha, I Made Artika,, Waras Nurcholis,

Isolation of yeast endophytes from healthy seeds of Capsicum annuum L. and assessment of their antimicrobial activity

Barbi Bhuyan, Purthimi Kungri Hansepi, Suraiya Akhtar, Raja Ahmed, Rafiul Amin Laskar, Kumanand Tayung

Metabolic profile, bioactivities, and variations in chemical constituents of essential oils of twenty mango ginger (Curcuma amada) accessions

Jyotirmayee Lenka, Snehalata Khuntia, Basudeba Kar, Suprava Sahoo

Phenolic compounds and in vitro antioxidant activity of spray-dried and freeze-dried aqueous extracts of sea cucumber (Holothuria tubulosa)

Fadna Aatab, Fatima Bellali, Fatima Zahra Aboudamia, Ahmed Errhif, Mariem Kharroubi

Phytomolecules against inflammation and fibrosis in immunoglobulin A nephropathy

Rajiv Jash,, Kousik Maparu, Sanket Seksaria, Sujan Banerjee, D. S. N. B. K. Prasanth, Himangshu Sekhar Maji

Evaluation of the vanillin treatment on migration and anchorage-independent growth of glioblastoma cell line

Megha Gautam, Reema Gabrani

Increasing polyphenol antioxidant in Orthosiphon stamineus Benth leaves with fermentation extraction by Saccharomyces cerevisiae ATCC-9763

Muhammad Aria Chandra, Khaswar Syamsu, Laksmi Ambarsari, Nurul Fatimah, Waras Nurcholis,

Phytochemical composition and antiproliferative activity of Opuntia elatior Mill.: In vitro and in silico studies on breast cancer cell line MCF-7

Foram Patel, Khushali Upadhyay, Denni Mammen, Elizabeth Robin, A.V. Ramachandran, Darshee Baxi

Bioactive properties of the extracts of peels, pomace, seeds, and essential oils of Citrus limon and Citrus aurantifolia

Folasade Oluwatobi, Olakunle Afolabi, Pius Okiki, Funmilayo Adeniyi, Oghenerobor Akpor

Molecular docking and simulation studies of medicinal plant phytochemicals with Leishmania donovani adenosylmethionine decarboxylase

Praffulla Kumar Arya, Krishnendu Barik, Ajay Kumar Singh, Anil Kumar

Assessment of biodegradation potential of lead-resistant bacteria isolated from polluted sites of Gomati River in Lucknow

Afreen Shahid, Farhan Ahmad, Chitranshu Pandey, Sunil Kumar, Aisha Kamal

High resolution-liquid chromatograph mass spectrometer characterization of bioactive compounds in pineapple wastes: Valorization of antioxidant and enzymatic activity

Suman Polaki, Sourav Nayak, K. Sampad Kumar, Rabi Prasad B

Optimization of enzyme-assisted extraction conditions for gamma-aminobutyric acid and polyphenols in germinated mung beans (Vigna radiata L.)

Anh Thuy Vu, Tuyen Chan Kha, Huan Tai Phan

Preparation of zinc oxide-carboxymethyl cellulose blended with cyclophosphamide for targeted drug delivery to lung adenocarcinoma cells

K. Harsha Prabha, Mahalakshmi Nannan, Sivaramakrishnan Venkatabalasubramanian

Rhizospheric microbiomes for agricultural sustainability

Ajar Nath Yadav,, Divjot Kour, Neelam Yadav

Thermal treatment and inhibition study with non-toxic product of polyphenol oxidase from Mediterranean Palm heart (Chamaerops humilis L.)

Abdelaziz Bouchaib, Abdellatif Ben Abdellah, Tarik Chafik, Amine Laglaoui, Abdelhay Arakrak, Mohamed Bakkali, Abdelaziz Benjouad, Amal Maurady

Assessing the role of temperature as an elicitor for indole-3-acetic acid production in cyanobacterial species

Priyanka Behera, Dhanesh Kumar,, Shubhashree Mahalik

Combinatorial network of transcriptional and post-transcriptional regulation in amyotrophic lateral sclerosis

Rupesh Kumar, Pammi Gauba, Shazia Haider

Efficacy of bacteriophage L522 against bacterial leaf blight of rice in Vietnam

Pham D.T. My,, Le T.T. Tien,, Le P. Nga,, To H. Ngoc,, Vo T. Phuc,, Hoang A. Hoang,

Solid-state fermentation of pigment producing endophytic fungus Fusarium solani from Madiwala lake and its toxicity studies

Bhoomika Prakash Poornamath, Suma Sarojini, Saranya Jayaram, Soma Biswas, Anand Kaloor, Mridul Umesh

Microbial diversity of river Ganga at Haridwar (Uttarakhand) through metagenomic approaches

Annapurna Katara, Sumit Chand, Harshvardhan Chaudhary, Harish Chandra, Ramesh Chand Dubey

In silico analysis of garlic phytochemicals binding affinities to skeletal muscle atrophy linked factors through molecular docking

Monika Monika, Sanjeev Gupta, Anita Dua, Ashwani Mittal

Screening and molecular characterization of phytase producer from north Gujarat region

Madhvi, Hardik Shah

Phenotypic characterization and genetic diversity of the Khiew-Phalee chicken (Gallus gallus): A fighting cock originating from Uttaradit, Thailand

Siriwadee Phromnoi, Preeda Lertwatcharasarakul, Wallaya Phongphaew, Pisit Poolprasert

Porphyridium sp. Microalgae as a source of polysaccharides

Yulia-Danae Boychenko, Ekaterina Budenkova, Nina Kostyushina, Olga Babich, Egor Kashinskikh

Identification of Orthosiphon stamineus from different phytogeographical zones in Indonesia by FTIR-fingerprinting and chemometrics

Kartini Kartini, Khusnul Khotimah, Nikmatul Ikhrom Eka Jayani, Finna Setiawan, Nina Dewi Oktaviyanti, Mochammad Arbi Hadiyat

Effect of farmers’ knowledge and production practices on the intensity of cassava bacterial blight in Western Kenya

Joe Habert Okoth, James Wanjohi Muthomi, Agnes Wakesho Mwang’ombe

A low-cost production, characterization, and application of raw starch degrading enzyme from the thermophilic filamentous bacterium, Laceyella sacchari P43

Wanthanee Khetkorn, Ausawadee Phonlamai, Chatsuda Sakdapetsiri, Vichien Kitpreechavanich, Thanasak Lomthong

Development and characterization of a lateral flow immunochromatographic assay test strip for rapid detection of rubella virus antigen

Huong Thu Ngo, Tho Tien Ngo, Son Van Tran, Yen Thu Tran, Hien Dang Nguyen, Phong Quoc Truong

Triphala extract Reduces Anti-apoptotic Protein (Mcl-1) Expression through the Activation of Caspase 3 in Hepatocellular Carcinoma Cells

Yoganchal Mishra, Rohit Raj Yadav, Kavita Verma, Neelam Yadav

Study of morphology and orchid mycorrhizal associations in Malaxis rheedei

B. S. Jyothsna, Radha Mahendran, Manish Raj Mishra, Sanjay Dey, Deepti Srivastava

Genetic dissimilarity, attributes association, and path analysis of sweet peppers

Jannatul Ferdousi, Mohammad Zakaria, Md. Azizul Hoque, Nasrin Akter Ivy, Satya Ranjan Saha, Md. Iqbal Hossain, Shila Pramanik, Dwipok Debnath Dwipok

Genome mining and AntiSMASH analysis of an Endophytic Talaromyces sp. reveal biosynthetic pathway gene clusters for novel bioactive compounds

Priyanka N. Shenoy, Sneha Bhaskar, M. Manu, M. P. Likitha, N. Geetha, Shailasree Sekhar, K Ramachandra Kini

Refined Evolutionary Trees Through an Exceptionally Compatible Alignment-Substitution Model

Ashish Runthala, K. Sowmya, Shamantha Nasika, Bhimavarapu Sai, Atanu Talukdar, Vijayakumar Rajendran, S. Karthikeyan, T. Silambarasan, Manmohan Sharma

The medicinal plant Berberis aristata and its endophytes for pharmacological applications: Current research and future challenges

Sheetal Sharma, M. V. N. L. Chaitanya, Sarika Sharma, Sanjeev Kumar, Sarvesh Rustagi, Sangram Singh, Sheikh Shreaz, Ashutosh Kumar Rai, Rajeshwari Negi, Ajar Nath Yadav,

Toxins in plant pathogenesis: Understanding the role of toxins in host-pathogen interaction

Seweta Srivastava, Akhilesh Chandrapati, Aakash Gupta, Meenakshi Rana, Arun Karnwal, Kanuri Komala Siva Katyayani, Raghavendra Reddy Manda, Dipshikha Kaushik, Shaptadvipa Bhattacharjee, Ravindra Kumar

Total phenolic content and antioxidant activities in methanol extracts of medicinal herbs from Indo-Gangetic plains of India

Umesh Kumar, Indrajeet Kumar, Prince Kumar Singh, Jay Shankar Yadav, Akanksha Dwivedi, Priyanka Singh, Saumya Mishra, Rajesh Kumar Sharma

A computational statistical approach to assess cowpea [Vigna unguiculata (L.) Walp.] cultivars diversity and select elite genotypes from Agro-morphological and biochemical traits

Noel Dougba Dago, Jean Simon Konan Assouman, Saraka Didier Martial Yao, Nafan Diarrassouba

Molecular, morphological, and biomolecular characterization of ethyl methanesulfonate-induced mutations in Aerides odoratum, an orchid

B. S. Jyothsna, Sanjay Dey, Venkataramanan S., Raghavendra L. S. Hallur, Deepti Srivastava

Impact of Jeevamrut formulations and biofertilizers on soil microbial and chemical attributes during potato cultivation

Rudra Pratap Singh Gurjar, Dashrath Bhati, Shailesh Kumar Singh

Optimizing solid-state fermentation for metabolite enrichment by Aspergillus tamarii on rice bran and wheat

Syaefudin Suminto,, Arthur Alba Huang, Uswatun Hasanah, Waras Nurcholis,

Standardization of reference genes for real-time PCR analysis of Vigna radiata L. under Agrobacterium tumefaciens infection

V. M. Nivya, Jasmine M. Shah

Formulation and effectivity of consortia of potential plant growth promoting endophytic bacteria from chickpea (Cicer arietinum)

Pradeep Kumar, Jitender Rathee, Simran Rani, Pooja Suneja

Biodiversity, mechanisms, and potential biotechnological applications of minerals solubilizing extremophilic microbes: A review

Rubee Devi, Tanvir Kaur, Rajeshwari Negi, Babita Sharma, Sohini Chowdhury, Monit Kapoor, Sangram Singh, Sarvesh Rustagi, Sheikh Shreaz, Pankaj Kumar Rai, Ashutosh Kumar Rai, Ashok Yadav, Divjot Kour, Ajar Nath Yadav

Plant growth-promoting rhizobacteria: Influence to abiotic stress tolerance in rice (Oryza sativa L.)

Trinayana Sonowal, Namrata Gupta, Sanjeev Kumar, Sarvesh Rustagi, Sangram Singh, Ashutosh Kumar Rai, Sheikh Shreaz, Rajeshwari Negi, Ajar Nath Yadav,

Systems biology approaches of Scopoletin reveal target potential biomarkers and its associated signaling pathways in colon cancer

Kunnathur Murugesan Sakthivel, Rajan Radha Rasmi, Loganathan Chandramani Priya Dharshini, Balasubramanian Ramesh

Variability in Indian wheat germplasm for important quality and physiological traits

Sabhyata Sabhyata,, Arun Gupta, Diwakar Aggarwal, Ratan Tiwari, Ruchika Sharma, Ankush Kumar, Gyanendra Singh

Screening and identification of heat tolerance in Indian wheat genotypes using generalized regression neural network (GRNN) model

Anil Kumar, Sadaf Fatima, Iffat Azim, Anshika Negi, Shalini Bhadola, Pooja Jha, Suboot Hairat

Infectious characteristics of some Vibrio spp. phages isolated in shrimp farming of the Mekong delta

Le Hoang Bao Ngoc,, Nguyen Thi Loan Anh, Vo Ngoc Tram Anh, Nguyen Thi Phuong Uyen, Le Viet Dung, Truong Thi Bich Van

Effects of liquid storage of buck semen at refrigeration temperatures on sperm viability and fertility to develop ready to use goat semen diluent

Manish Kumar,, Ravi Ranjan, Alok Bhardwaj

Befunolol as a potential inhibitor of glycogen phosphorylase: an in silico approach

Pavani Nadavapalli, Padmini Nadavapalli, Kavya Sritha Bojja, Kavishankar Gawli

Role of zinc oxide nanoparticles in alleviating sodium chloride-induced salt stress in sweet basil (Ocimum basilicum L.)

Syed Aiman Hasan, Adnan Khan, Mohd Irfan

Pharmacological and biotechnological overview of Sauropus androgynus L. Merr.: an underexploited perennial shrub

Dhaneswar Swain, Bijaya Kumar Sahoo, Ajaya Pattanaik, Subrat Kumar Mahapatra, Gyana Ranjan Rout

Prediction of HIV drug resistance through in silico approach

Tammanna Bhajantri, S. Pushkala

The induction of metallothionein by sulforaphane reduces iron toxicity via Nrf2

Amanda Putri Elvandari, Ferbian Milas Siswanto, Susumu Imaoka

Technological advancement in the development of monoclonal antibody therapies: Present, past, and future

Harit Kasana,, Harish Chander, Ashwani Mathur

Omics technologies for understanding the plant–fungal endophyte interactions: crop improvement for future security

Tanvir Kaur, Rajeshwari Negi, Babita Sharma, Simranjeet Kaur, Sofia Sharief Khan, Divjot Kour, Sangram Singh, Sarvesh Rustagi, Sheikh Shreaz, Neelam Yadav, Manish Kumar, Ashutosh Kumar Rai, Ajar Nath Yadav

Assessment of protein profile and antibacterial activity in hemolymph of Bacillus thuringiensis-immunized muga silkworm

Shibani Kalita, Sanghamitra Saharia, Dimpimoni Kalita, Dhirunabh Swargiary, Sunayan Bardoloi

Exploring Bougainvillea glabra flowers: a promising source of natural antimicrobial and anticancer agents

Wanchat Sirisarn, Auemphon Mordmuang, Kankamol Kerdkumthong, Sompop Saeheng,,

Molecular characterization of the ligninolytic Sundarban mangrove isolate Stenotrophomonas maltophilia GD1 and its promising applications in the dye and fiber industry

Somnath Das, Mitun Sen, Nilothpal Sinha, Suryasarathi Kumar, Noiranjana Dasgupta, Dipankar Ghosh

Characterization of acid-soluble collagen from peanut worm (Siphonosoma australe) and its inhibitory activity of cyclooxygenase-2

Suwarjoyowirayatno,, Chusnul Hidayat, Tutik Dwi Wahyuningsih, Retno Indrati

The puzzle of pheromones in nature and their mysterious influence: a comprehensive review

Mighila Rixon, Manikantan Pappuswamy, Aditi Chaudhary, Sana Syed Ansar

Occurrence and life cycle of broomrape (Phelipanche aegyptiaca Pers.) on mustard (Brassica campestris L.) in Northeastern regions of Rajasthan

Sarita Kumari, Ridhi Joshi, Rishi Kesh Meena, Preeti Mishra

Checklist of pteridophytes from the district Jalpaiguri, West Bengal, India

Manas Dey, Ashish K. Soni, Syed Shahab Ahmed, Anup Kumar Sarkar

Evaluation and characterization of endophytic bacteria from Capparis decidua (Forssk.) Edgew. for their antifungal and antioxidant activities

Sudesh Kumari, Prity Gulia, Pooja Choudhary, Ritu Pasrija, Mehak Dangi, Anil Kumar Chhillar

Phosphate and potassium solubilizing Siccibacter colletis promotes wheat growth, yield, and nutrient uptake

Pawan Kumar, Sandeep Kumar, Parkriti Jhilta, Vikram Poria, Rajpaul Yadav, Surender Singh

Traditional use, phytoconstituents, and pharmacological effects of Persea americana: A recent review

José Gilberto Gavídia-Valencia, Edmundo Arturo Venegas-Casanova, Miriam E. Gutiérrez-Ramos, Roger A. Rengifo-Penadillos, Cleydy Jaysia Cajamarca-Porras, Ruben J. Aro-Díaz, José Mostacero-León, Ricardo Diego Duarte Galhardo De Albuquerque

Leaf area index, quality, and nutrient uptake in wheat (Triticum aestivum L.) affected by different planting patterns and nitrogen levels

Harmanpreet Kaur Gill, Ujagar Singh Walia

Enhanced quinalphos degradation using immobilized cells of Enterobacter cloacae isolated from farm land soil of Vazhayur Panchayath, Malappuram District, Kerala

Nhattuketty Krishnan Shainy, Asha Bhakthavalsalam, Shabanamol Subaidabeevi, Farha Baramy, Aswathi Ayiliath, Kulandhaivel Murugesan

In vitro production of gibberellic acid and fusaric acid by Fusarium spp. and their role in bakanae disease development

Asmaul Husna,, Md. Asaduzzaman Miah, Latiffah Zakaria, Nik Mohd Izham Mohamed Nor

Comparative and evolutionary analyses of cyclophilins in Cucumis sativus, Phaseolus vulgaris, and Vitis vinifera

Neelam Yadav, Neha Bharill, Yashi Gautam, Rishiraj Raghuvanshi, Shikha Meda, Krishnan Hajela, Bhushan Dholakia, Aruna Tiwari, Prashanth Suravajhala, Milind B. Ratnaparkhe

Isolation and identification of indigenous lactic acid bacteria with inhibitory activity against Aeromonas hydrophila in Vinh Long province

Thi Van Cao Quach, Thuy Phuong Nguyen, Tat Quoc Truong, Nguyen Bao Trung

Assessment of genetic stability of micropropagated Curculigo latifolia from Indonesia by RAPD and ISSR

Wirdhatul Muslihatin,, Triono Bagus Saputro, Anjar Tri Wibowo, Yosephine Sri Wulan Manuhara

Estimation of seaweed extract and micronutrient potential to improve net returns by enhancing yield characters in tomato using correlation analysis

Diksha Choudhary, Monisha Rawat, Vinay Kumar Mashkey, Vinit Sharma, Parvesh Kundu

Optimizing cultivation conditions for enhanced productivity Limnothrix planctonica through pH variation and light quality

Prachaya Chamarat, Nuttha Sanevas

Colonization pattern of arbuscular mycorrhizal fungi in invasive plant species of tropical dry deciduous forest of Belgahna range of Central India

Sakshi Gautam, Bhaskar Chaurasia

Endophytic microbiomes for agricultural sustainability

Ajar Nath Yadav

A review on ecology, distribution, phytochemistry, and pharmacological properties of the medicinally important plant Pterocarpus santalinus L. (Red sanders)

M. Keshavamurthy, N. Gayathri Devi, A. C. Manjula, K. S. Shubha, C. L. Deepashree, P. Janani, R. M. Archana

Streptomyces as endomicrobiome: Potential bioinoculants for agricultural sustainability

Rangasamy Kirubakaran, Nowsheen Shameem, Elumalai Saranya, Krishnan Meenambigai, Ramu Dhanasekar, Javid Ahmad Parray, Neelam Yadav,, Sangram Singh, Sarvesh Rustagi, Paridhi Puri, Babita Sharma, Rajeshwari Negi, Ajar Nath Yadav

Mutualistic synergy between Eudrilus eugeniae gut microbiota and Beta vulgaris root endophytes in organic matter decay

Pushpa Reddy, T. K. Suma, Amlan Hossain, Sudarsan Sarkar

The protective action of a novel Dinb protease against diarrhea infection in Drosophila Melanogaster

Jyoti Guleria, Mohammad Rashid Khan, Minhaj Ahmad Khan

Monoclonal antibodies for diagnosis of viral diseases: a comprehensive review

Abhishek Kasana, Aishwarya Dwivedi, Vaishali Verma

Genetic diversity investigation of some Darjeeling Himalayan Rhododendron species based on RAPD method

Animesh Mondal, Kalyan Kumar De

DNA barcoding and phylogenetic analysis of Indian Phyllanthus using nrITS and chloroplast rbcL gene sequences for molecular identification

Raghavendra Polasam, Pushpalatha Ganesh, Gururaj Chalageri, R. Kannan, U. V. Babu