Research Article | Volume: 4, Issue: 2, March-April, 2016

Syntrophic microbial system for ex-situ degradation of paddy straw at low temperature under controlled and natural environment

Livleen Shukla Archna Suman Priyanka Verma Ajar Nath Yadav Anil Kumar Saxena   

Open Access   

Published:  Apr 21, 2016

DOI: 10.7324/JABB.2016.40205

The syntrophic microbial application for lignocellulosic biodegradation and subsequent transformation into compost provides an alternative strategy against burning and disposing post harvested agricultural biomass which is of vital importance in agriculture used as compost. Biodegradation process is hindered during winter season, as the microorganisms involved in lignocellulose biodegradation slows down their metabolism due to unfavourable growth conditions at low temperatures. In order to intensify the composting process at low temperature, psychrotrophic microbes were isolated and characterized for lignocellulosic hydrolytic potential specifically at low temperatures. Among the isolated microbes, four efficient lignocellulolytic psychrotrophic microbes (Eupenicillium crustaceum, Paceliomyces sp., Bacillus atropheus and Bacillus sp.) and commercial fungal consortia (Aspergillus awamori, Aspergillus nidulans, Trichoderma viride and Phanerochaete chrysosporium) were used in present study. It was found that psychrotrophic microbes along with the commercial fungal consortium enhanced the composting process at low temperature. These psychrotrophic and mesophilic microbial consortium can be used for degradation of agri-residues and conversion to a value added product like compost, which helps in enhancing soil fertility and decreasing environmental pollution caused by burning of agrowastes. This is the first report for biodegradation of paddy straw by psychrotrophic microbes at low temperatures.

Keyword:     Agriculture waste Composting Microbial consortia Psychrotrophic microbes.


Shukla L, Suman A, Verma P, Yadav AN, Saxena AK. Syntrophic microbial system for ex-situ degradation of paddy straw at low temperature under controlled and natural environment. J App Biol Biotech. 2016; 4 (02): 030-037. DOI: 10.7324/JABB.2016.40205

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Kadam, KL, Forrest, LH, Jacobson, WA. Rice straw as a lignocellulosic resource: collection, processing, transportation, and environmental aspects. Biomass Bioenerg. 2000; 18: 369-389.

2. Gadde, B, Bonnet, S, Menke, C, Garivait, S. Air pollutant emissions from rice straw open field burning in India, Thailand and the Philippines. Environ Poll. 2009; 157: 1554-1558.

3. Hatamoto, M, Tanahashi, T, Murase, J, Matsuya, K, Hayashi, M, Kimura, M, Asakawa, S. Eukaryotic communities associated with the decomposition of rice straw compost in a Japanese rice paddy field estimated by DGGE analysis. Biol Fert Soils. 2008; 44: 527-532.

4. Ahmad, R, Jilani, G, Arshad, M, Zahir, ZA, Khalid, A. Bio-conversion of organic wastes for their recycling in agriculture: an overview of perspectives and prospects. Ann Microbiol. 2007; 57: 471-479.

5. Blackmore, E, Keeley, J. Assessing the benefits of sustainability certification for small-scale farmers in Asia. IIED Natural Resource Issues, London. 2012.

6. Kumar, A, Gaind, S, Nain, L. Evaluation of thermophilic fungal consortium for paddy straw composting. Biodegradation. 2008; 19: 395-402.

7. Sharma, RK, Arora, DS. Biodegradation of paddy straw obtained from different geographic locations by means of Phlebia spp. for animal feed. Biodegradation. 2011; 22: 143-152.

8. Kausar, H, Sariah, M, Saud, HM, Alam, MZ, Ismail, MR. Isolation and screening of potential actinobacteria for rapid composting of rice straw. Biodegradation. 2011; 22: 367-375.

9. Barrington, S, Choinière, D, Trigui, M, Knight, W. Effect of carbon source on compost nitrogen and carbon losses. Bioresour Technol. 2002; 83: 189-194.

10. Hassen, A, Belguith, K, Jedidi, N, Cherif, A, Cherif, M, Boudabous, A. Microbial characterization during composting of municipal solid waste. Bioresour Technol. 2001; 80: 217-225.

11. Yadav, AN, Sachan, SG, Verma, P, Tyagi, SP, Kaushik, R, Saxena, A. Culturable diversity and functional annotation of psychrotrophic bacteria from cold desert of Leh Ladakh (India). World J Microbiol Biotechnol. 2015; 31: 95-108.

12. Yadav, AN, Verma, P, Kumar, M, Pal, KK, Dey, R, Gupta, A, Padaria, JC, Gujar, GT, Kumar, S, Suman, A, Prasanna, R, Saxena, AK. Diversity and phylogenetic profiling of niche-specific Bacilli from extreme environments of India. Ann Microbiol. 2015; 65: 611-629.

13. Zhou, X, Chen, H, Li, Z. CMCase activity assay as a method for cellulase adsorption analysis. Enzyme Microb Tech. 2004; 35: 455-459.

14. Wejse, PL, Ingvorsen, K, Mortensen, KK. Purification and characterisation of two extremely halotolerant xylanases from a novel halophilic bacterium. Extremophiles. 2003; 7: 423-431.

15. Miller, GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analyt Chem. 1959; 31: 426-428.

16. Yadav, AN, Sachan, SG, Verma, P, Kaushik, R, Saxena, AK. Cold active hydrolytic enzymes production by psychrotrophic Bacilli isolated from three sub-glacial lakes of NW Indian Himalayas. J Basic Microbiol. 2015; doi:10.1002/jobm.201500230.

17. Suman, A, Verma, P, Yadav, AN, Saxena, AK. Bioprospecting for extracellular hydrolytic enzymes from culturable thermotolerant bacteria isolated from Manikaran thermal springs. Res J Biotechnol. 2015; 10: 33-42.

18. Verma, P, Yadav, AN, Khannam, KS, Kumar, S, Saxena, AK, Suman, A. Molecular diversity and multifarious plant growth promoting attributes of Bacilli associated with wheat (Triticum aestivum L.) rhizosphere from six diverse agro-ecological zones of India. J Basic Microbiol. 2016; 56: 44-58.

19. Yadav, AN, Sachan, SG, Verma, P, Saxena, AK. Prospecting cold deserts of north western Himalayas for microbial diversity and plant growth promoting attributes. J Biosci Bioeng. 2015; 119: 683-693.

20. Verma, P, Yadav, AN, Khannam, KS, Mishra, S, Kumar, S, Saxena, AK, Suman, A. Appraisal of diversity and functional attributes of thermotolerant wheat (Triticum aestivum L.) associated bacteria from peninsular zone of India. Saudi J Biol Sci. 2016. doi:10.1016/j.sjbs.2016.01.042.

21. Shukla, L, Senapati, A, Tyagi, S, Saxena, AK. Economically viable mass production of lignocellulolytic fungal inoculum for rapid degradation of agrowaste. Curr Sci. 2014; 107: 1701.

22. Walkley, A, Black, IA. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Sci. 1934; 37: 29-38.

23. Black, C, Evans, D, White, J, Ensminger, L, Clark, F. Method of soil analysis. Part II. Am Soc Agron, Madison, USA. 1965; 1572.

24. Zucconi, F, Monaco, A, Debertoldi, M. Biological evaluation of compost maturity. Biocycle. 1981; 22: 27-29.

25. MacGregor, S, Miller, F, Psarianos, K, Finstein, M. Composting process control based on interaction between microbial heat output and temperature. Appl Environ Microbiol. 1981; 41: 1321-1330.

26. Paredes, C, Roig, A, Bernal, M, Sánchez-Monedero, M, Cegarra, J. Evolution of organic matter and nitrogen during co-composting of olive mill wastewater with solid organic wastes. Biol Fert Soils. 2000; 32: 222-227.

27. Chaturvedi, S, Kumar, A, Singh, B, Nain, L, Joshi, M, Satya, S. Bioaugmented composting of Jatropha de‐oiled cake and vegetable waste under aerobic and partial anaerobic conditions. Journal of basic microbiology. 2013; 53: 327-335.

28. Rashad, FM, Saleh, WD, Moselhy, MA. Bioconversion of rice straw and certain agro-industrial wastes to amendments for organic farming systems: 1. Composting, quality, stability and maturity indices. Bioresour Technol. 2010; 101: 5952-5960.

29. Barrena, R, Vázquez, F, Sánchez, A. Dehydrogenase activity as a method for monitoring the composting process. Bioresour Technol. 2008; 99: 905-908.

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