Research Article | Volume: 4, Issue: 2, March-April, 2016

Transcriptional expression of three putative pathogenesis-related proteins in leaves of rubber tree (Hevea brasiliensis) inoculated with Neofusicoccum ribis

A. I. C. Nyaka Ngobisa Godswill Ntsomboh-Ntsefong Wong Mui Yun M. Z. Dzarifah P. A. Owona Ndongo   

Open Access   

Published:  Apr 21, 2016

DOI: 10.7324/JABB.2016.40202
Abstract

Pathogenesis related-proteins (PR-proteins) and enzymes are important tools for understanding the molecular markers of plant response to external factors. In this study, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was optimized to detect the expressions of two PR-proteins and one enzyme extracted from the leaf RNA of two rubber clones (PB 350 and RRIM 2002) at four-period intervals. The expression patterns of enzymes β-1,3-glucanase (Glu), chitinase (Chit), and phenylalanine ammonia lyase (PAL) in the leaf tissues of the clones RRIM 2002 and PB 350 to Neofusicoccum ribis were expressed by up and down regulations and varied with time. The trial inoculation indicated that the gene expressions were significantly higher in tolerant clones (RRIM 2002) than in the susceptible clones (PB 350). Gene expression analysis of the biomarkers revealed that the earlier detection markers in infections occurred within 5–10 days post infection and showed significant correlation with disease development, ranging from 1.67–3% in the RRIM 2002 clone and approximately 8.33% in the PB 350 clone. This is the first study on the infection and up-regulation of gene expression in rubber leaves infected with N. ribis. This study showed the importance of exploring the SK10 isolate as an indicator of infection ability and positive fungal–host interaction.


Keyword:     Gene expression RT-PCR Neofusicoccum ribis Pathogenesis-related protein.


Citation:

Nyaka Ngobisa AIC, Ntsomboh-Ntsefong G, Wong MY, Dzarifah MZ, Owona Ndongo PA. Transcriptional expression of three putative pathogenesis-related proteins in leaves of rubber tree (Hevea brasiliensis) inoculated with Neofusicoccum ribis. J App Biol Biotech. 2016; 4 (02): 011-015. DOI: 10.7324/JABB.2016.40202

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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