Secondary metabolite profiles, antimicrobial and antioxidant activities of callus, and leaves extract of Piper sarmentosum Roxb.

Junairiah Junairiah Listijani Suhargo Tri Nurhariyati Nabilah Istighfari Zuraidassanaaz   

Open Access   

Published:  May 02, 2024

DOI: 10.7324/JABB.2024.190553

This research was aimed to investigate the effect of different combination concentrations of 2,4-diclorophenoxyacetic acid (2,4-D) and benzylaminopurine (BAP) on callus induction, profiles of secondary metabolites, antimicrobial, and antioxidant activities from Piper sarmentosum leaves. These leaves explants were cultured on Murashige and Skoog (MS) medium supplemented with different combination concentrations of 2,4-D and BAP (0.5; 1.0; 1.5; 2.0; 2.5 mg/L) during 6-week culture callus period. Result showed that the morphology of callus grown during this study was compact in various colors appearance, such as light brown, brownish, and dark brown. The fastest mean of callus induction time was 0.5 mg/L 2,4-D and 1.5 mg/L BAP (10 days). The highest callus fresh weight was 1.5 mg/L 2,4-D and 2.0 mg/L BAP (0.350 g). The combination concentration of 1.5 mg/L 2,4-D and 1.5 mg/L BAP was the highest callus dry weight (0.078 g), this treatment was contained 11 different compounds, which were dominated by cyanoacetamide (25.08%) and diisooctyl-phthalate (21.81%). Myristicin and propanamide compounds were also identified in the methanol extract of P. sarmentosum callus. Methanolic extract of callus P. sarmentosum from the optimum treatment had the highest antioxidant activity (26.709 μL/mL) and antimicrobial activity against three test microbes.

Keyword:     Antimicrobial Antioxidant Callus culture Piper sarmentosum Roxb. Leaves Secondary metabolites profile


Junairiah J, Suhargo L, Nurhariyati T, Zuraidassanaaz NI. Secondary metabolite profiles, antimicrobial and antioxidant activities of callus, and leaves extract of Piper sarmentosum Roxb. J App Biol Biotech. 2024. Online first.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Dehydration kinetics of green banana slices, characterization of optimized product based on physicochemical, nutritional, optical, and sensory attributes

Ram Kaduji Gadhave, Ravneet Kaur, Rahul Das, Kamlesh Prasad

Assessment of morpho-agronomic and yield attributes in gamma-irradiated mutants of Kalanamak rice (Oryza sativa L.)

Tanmai Mishra, Anjali Singh, Virendra Kumar Madhukar, Ashutosh Kumar Verma, Shambhavi Mishra, Rajveer Singh Chauhan

Metabolic profile, bioactivities, and variations in chemical constituents of essential oils of twenty mango ginger (Curcuma amada) accessions

Jyotirmayee Lenka, Snehalata Khuntia, Basudeba Kar, Suprava Sahoo

Phenolic compounds and in vitro antioxidant activity of spray-dried and freeze-dried aqueous extracts of sea cucumber (Holothuria tubulosa)

Fadna Aatab, Fatima Bellali, Fatima Zahra Aboudamia, Ahmed Errhif, Mariem Kharroubi

Optimization of pasteurization process of the ready-to-drink beverage from Hong Quan (Flacourtia jangomas) fruit by response surface methodology

Tan Duy Nguyen,, Tuyen Thi Xuan Vo,, Khang Nghia Tran,

Increasing polyphenol antioxidant in Orthosiphon stamineus Benth leaves with fermentation extraction by Saccharomyces cerevisiae ATCC-9763

Muhammad Aria Chandra, Khaswar Syamsu, Laksmi Ambarsari, Nurul Fatimah, Waras Nurcholis,

Phytochemical composition and antiproliferative activity of Opuntia elatior Mill.: In vitro and in silico studies on breast cancer cell line MCF-7

Foram Patel, Khushali Upadhyay, Denni Mammen, Elizabeth Robin, A.V. Ramachandran, Darshee Baxi

Bioactive properties of the extracts of peels, pomace, seeds, and essential oils of Citrus limon and Citrus aurantifolia

Folasade Oluwatobi, Olakunle Afolabi, Pius Okiki, Funmilayo Adeniyi, Oghenerobor Akpor

High resolution-liquid chromatograph mass spectrometer characterization of bioactive compounds in pineapple wastes: Valorization of antioxidant and enzymatic activity

Suman Polaki, Sourav Nayak, K. Sampad Kumar, Rabi Prasad B

Recent important insight into nutraceuticals potential of pigmented rice cultivars: A promising ingredient for future food

Le Thi Kim Loan, Bui The Vinh, Ngo Van Tai

Solid-state fermentation of pigment producing endophytic fungus Fusarium solani from Madiwala lake and its toxicity studies

Bhoomika Prakash Poornamath, Suma Sarojini, Saranya Jayaram, Soma Biswas, Anand Kaloor, Mridul Umesh

Elucidation of antioxidant compounds recovery capacity from “Cam” purple rice bran by different sustainable extraction techniques

Le Thi Kim Loan, Bui The Vinh, Ngo Van Tai

Effect of nutrient media enhanced with plant growth regulators on genetic stability in sub-cultures of Digitalis purpurea callus

Mohammed Ahmed AL-Oqab, Salim Zaid, Youssef Al-Ammouri, Shawqi H. Alawdi