Research Article | Volume: 4, Issue: 1, Jan-Feb, 2016

Effects of Plant Growth Regulators on in-vitro regeneration of a potential medicinal plant -Scilla hyacinthina (Roth.)J.F. Macbr

K. Kamaleswari V. Nandagopalan A. Lakshmi Prabha   

Open Access   

Published:  Feb 19, 2016

DOI: 10.7324/JABB.2016.40108
Abstract

A suitable plant regeneration protocol has been developed from scale leaf and bulb scale explants of Scilla hyacinthina, a medicinally important plant species belonging to the family liliaceae. The maximum number of shoots (13.2), with average length 6.5 cm, was achieved with medium containing 1.5 mg/l TDZ. Rooting of shoots was achieved on half-strength MS medium supplemented with 1.5 mg/1 IAA.


Keyword:     Scilla hyacinthinaTDZIAABulb scaleScale leafMicroproagation.


Citation:

Kamaleswari K, Nandagopalan V, Prabha AL. Effects of Plant Growth Regulators on in-vitro regeneration of a potential medicinal plant-Scilla hyacinthina (Roth.)J.F. Macbr. J App Biol Biotech. 2016; 4 (01): 043-046. DOI: 10.7324/JABB.2016.40108

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text
Reference

1. Mathew KM. The flora of tamilnadu Carnatic, part three, monocoteledons, India. 1983; 1646-1647.

2. Ghurde OM and Malode SN. Phytochemical screening and assessment of biomolecules compounds in Scilla hyacinthina (Roth) Macbr. Bulb. J.GlobalBiosci. 2014; 3(5): 866-871.

3. Sudhersen C and Padmanabhan D. In vitro propagation of Scilla hyacinthina (Roth.) Macbr. Curr. Sc. 1993; 64: 710

4. Akindel AJ and Adeyemi OO. Antinflammatoryactivity of the aqueous leaf extract of Byrsocarpuscoccineus. Fitoterapia. 2007; 78: 25-28.

5. Anis and Faisal. In vitro regeneration and mass multiplication of Psoraleacorylifolia – An endangered medicinal plant. Ind. J. Biotech. 2005; 4:261-264

6. Gupta P, Sharma AK and Chartuvedi HC. Multiplication of Liliumlongiflorum thumb. By aseptic culture of bulb scale. Ind.J.Exp.Biol. 1978; 16940-942.

7. Nhut DT, Le BV, Fukai S, Tanak M and VanTT. Effectsof activated charcoal,explant size, explant position and sucrose concentration on plant and shoot regeneration of Longiflorum via young stem. Plant Growth Reg. 2001; 33: 59 65.

8. Wala BB and Jarai TY. Micropropagation of an endangered medicinal plant CurculigoorchioidsGaertn. Pl. Tissue cul. 2003; 13(1): 13-19.

9. Chakravarthy B and Sen S.1 Regeneration through somatic embryogenesis from anther explants of Scillaindica (Roxb.) Baker. Plant cell tissue and org cult.1989; 19:71-75.

10. Langens –Gerrits M, Albers M and De klerkGJ,. Hot water treatment before tissue culture reduce initial contamination in lilium and Acer. Plant cell tissue and org cult 1998; 52:75-77.

11. Ziv M, Lilienkipnis H. Bud regeneration from inflorescence explant for rapid propagation of geophytes. In vitro Pl.Cell Report. 2000; 19:845-850.

12. Lin YS and Kwan SH. Recent advances of in vitro embryogenesis of monocotyledon and dicotyledon embryogenesis. 2012; 269-296.

13. Mokhtari A, Alizadeh H, Samad YB, Omidi M, Otroshy M and Moeini Z. Effect of plant growth regulators on direct shoot regeneration of wheat immature embryonic explants. J. Agri. Eng. Biotech. 2013; 3:74-80.

14. Alizadeh H, Naghavi MR, Omidi M and Saatian B. Effect of plant growth regulation on direct shoot regeneration of wheat (Triticumaestivum). 2004; Aus.Agrono. Conf.

15. Mirici S, Parmaksiz I, Ozcan S, Sancakc, Uranbey S, Sarihan OE, Gumuscu A, GurbuzB,andArslan N. Efficient in vitro bulblet regeneration from immature embryos of endanged Sternbergia fischeriana. Plant cell tissue and org cult. 2005; 80: 239-246.

16. UranbeyS. Stimulating effects of different basal media and cytokinine types on regeneration of endemic and endanged Muscariaucheri. Arch Biol. Sci. Belgrade. 2010; 62(3):663-667.

17. Yanagawa T and Osaki Y.In vitro propagation of bulblets and elimination of viruses by bulb scale culture of Hippeastrumhybridum bulbs. Pl.Tissuecul. 1996; 13(2): 147-152.

18. Wilson PJ. The concept of a limiting rooting morphogen in woody stem cuttings. J. Hortic. Sci.1994;69:591-600.

Article Metrics

178 Absract views 201 PDF Downloads 379 Total views

Related Search

By author names

Citiaion Alert By Google Scholar


Similar Articles