Research Article | Volume: 4, Issue: 1, Jan-Feb, 2016

Medium Formulation and its optimization for increased protease production by Penicillium sp. LCJ228 and its potential in blood stain removal

V. Benluvankar S. Evelyne Priya J. Joel Gnanadoss   

Open Access   

Published:  Feb 19, 2016

DOI: 10.7324/JABB.2016.40104

The production of protease by Penicillium sp. LCJ228 was optimized under submerged fermentation. Nutritional and physical factors that influence protease production were optimized by one factor at a time (OFAT) method in order to achieve high yield of protease. Maximum protease production was obtained on the 4th day in a liquid medium containing glucose (15 g/L), yeast extract (15 g/L), black gram husk (10 g/L) with an initial pH of 10 and an inoculum size of 2 g/L of fungal mycelium. About 2.4 fold increase in protease production was observed in the optimized medium. The maximally yielded crude protease was then precipitated and characterized by SDS-PAGE and native-PAGE. The molecular weight of the ammonium sulphate precipitated protease was ~66 to 70 kDa and native-PAGE showed three isoenzymes. The crude and precipitated protease had the ability to completely remove blood stains on cotton fabric indicating its potential to be used as a stain remover in detergents.

Keyword:     Protease Penicillium sp. submerged fermentation conventional optimization blood stain removal.


Benluvankar V, Priya SE and Gnanadoss JJ. Medium Formulation and its optimization for increased protease production by Penicillium sp. LCJ228 and its potential in blood stain removal. J App Biol Biotech. 2016; 4 (01): 020-026. DOI: 10.7324/JABB.2016.40104

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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