Research Article | Volume: 4, Issue: 1, Jan-Feb, 2016

Production of exopolysaccharides by the cyanobacterium Anabaena sp. BTA992 and application as bioflocculants

Romi Khangembam Onkar Nath Tiwari Mohan Chandra Kalita   

Open Access   

Published:  Feb 19, 2016

DOI: 10.7324/JABB.2016.40102
Abstract

Bioflocculant exopolysaccharide (EPS) production by ten (10) cyanobacterial strains comprising of five (05) heterocystous and five (05) non-heterocystous strains during their photoautotrophic growth was investigated. Highest level of EPS was produced by Anabaena sp. BTA992 among the investigated strains with high flocculation capacity. The cyanobacterial EPS consisted of soluble protein and polysaccharide that included substantial amounts of neutral sugars and uronic acid. The flocculant bound a cationic dye, alcian blue, indicating it to be polyanionic. EPS production was maximum during stationary growth phase and late phase of growth at pH 8.5. The strain was identified by 16S rRNA typing and GenBank accession number was obtained as KJ830950. The investigation revealed that this Anabaena sp. could be a potential candidate for the commercial EPS production and might be utilized in applications as an alternative to synthetic flocculants.


Keyword:     Cyanobacteria exopolysaccharide flocculants 16S rRNA typing pH.


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

Reference

1. Thajuddin N, Subramanian G. Cyanobacterial biodiversity and potential applications in biotechnology. Current Science. 2005; 89: 47-57.

2. Sutherland IW. Novel and established applications of microbial polysaccharides. Trends in Biotechnology. 1998; 16:41-46.

3. Itoh H, Noda H, Amano H, Zhuaug C, Mizuno T, Ito H. Antitumor activity and immunological properties of marine algal polysaccharides, specially fucoidan, prepared from Sargassum thunbergii of Phaeophyceae. Anti cancer Research. 1993; 13, 2045-2052.

4. Herrero A, Muro-Pastor A, Flores E. Nitrogen control in cyanobacteria. Journal of Bacteriology. 2001; 183, 411-425.

5. Shah V, Ray A, Garg N, Madamwar D. Characterization of the extracellular polysaccharide produced by a marine cyanobacterium, Cyanothece sp. ATCC 51142, and its exploitation toward metal removal from solutions. Current Microbiology. 2000; 40, 274-278.

6. Stanier RY, Kunisawa R, Mandel M, Cohen-Baziere G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriology Reviews. 1971; 35: 171-205.

7. Tiwari ON, Romi KH, Minerva SH, Aribam SS, Gunapati O, Jerry JB. Characterization and Optimization of Bioflocculant Exopolysaccharide Production by Cyanobacteria Nostoc sp. BTA97 and Anabaena sp. BTA990 in Culture Conditions. Applied Biochemistry and Biotechnology. 2015; 176(7): 1950-1963.

8. Klock JH, Weiland A, Seifert R, Michaeli W. Extracellular polymeric substances (EPS) from cyanobacterial mats: characterization and isolation method optimization. Marine Biology. 2007; 152: 1077-1085.

9. Bar-Or Y, Shilo M. Characterization of macromolecular flocculants produced by Phormidium sp. strain J and by Anabaenopsis circularis PCCC 6720. Applied and Environmental Microbiology. 1987; 53: 2226-2230.

10. Spiro RG. Analysis of sugars found in glycoproteins. Method in Enzymology. 1966; 8: 3-26.

11. Herbert TD, Phipps PJ, Strange RE. Chemical analysis of microbial cells. In: Methods of Microbiology (Morris, J.R. Ribbon, D.W. editors), 1971; 209-234, Academic Press, New York.

12. Galambos JT. The reaction of carbazole with carbohydrates. 1. Effect of borate and sulfamate on the carbazole color of sugars. Analytical Biochemistry. 1967; 19: 119-132.

13. Avijeet SO, Oinam G, Singh KO, Tiwari ON. Isolation of fresh water cyanobacterial DNA of north east India by modified xanthogenate method. International Journal of Research in BioSciences. 2013; 2(2): 75-82.

14. Nubel U, Garcia-Pichel F, Muyzer G. PCR primers to amplify 16S rRNA gene from cyanobacteria. Applied and Environmental Microbiology. 1997; 63(8): 3327-3332.

15. Raus Madieldo P, De Los Reyes Gavilan CG. Invited review: methods for the screening, isolation and characterization of exopolysaccharides produced by lactic acid bacteria. Journal of Dairy Science. 2005; 88: 843-856.

16. Sutherland IW. Structure-function relationships in microbial exopolysaccharides. Biotechnology Advances. 1994; 12: 393-448.

17. Ohki K, Nguyen QT, Yoshikawa S, Kanesaki Y, Okajima M, Kaneko T, Tran HT. Exopolysaccharide production by a unicellular freshwater cyanobacterium Cyanothece sp. isolated in a rice field of Vietnam. Journal of Applied Phycology. 2014; 26(1): 265-272.

18. De Philippis R, Colica G, Micheletti E. Exopolysaccharide-producing cyanobacteria in heavy metal removal from water: molecular basis and practical applicability of the biosorption process. Applied Microbiology and Biotechnology. 2011; 92: 697-708.

19. West TP. Effect of carbon source on polysaccharide production by alginate-entrapped Aureobasidium pullulans ATCC 42023 cells. Journal of Basic Microbiology. 2011; 51: 673-677.

20. Parikh A, Madamwar D. Partial characterization of extracellular polysaccharides from cyanobacteria. Bioresource Technology. 2006; 97: 1822-1827.

21. De Philippis R, Margheri MC, Pelosi E, Ventura S. Exopolysaccharide production by a unicellular cyanobacterium isolated from a hypersaline habitat. Journal of Applied Phycology. 1993; 5: 387-394.

22. Khattar JS, Singh DP, Jindal N, Kaur N, Singh Y, Rahi P, Gulati A. Isolation and characterization of exopolysaccharides produced by the cyanobacterium Limnothrix redekei PUPCCC 116. Applied Biochemistry and Biotechnology. 2010; 162: 1327-1338.

23. Tischer RG, Davis EB. The effect of various nitrogen sources upon the production of extracellular polysaccharide by the blue-green alga Anabaena flosaquae A-37. Journal of Experimental Botany. 1971; 22: 546-551.

24. Li P, Harding SE, Liu Z. Cyanobacterial Exoplysaccharides: their nature and potential biotechnological applications. Biotechnology and Genetic Engineering. 2001; 18: 375-404.

25. Singh S, Das S. Screening, production, optimization and characterization of cyanobacterial polysaccharide. World Journal of Microbiology and Biotechnology. 2011; 27: 1971-1980.

26. Kumar AS, Mody K, Jha B. Bacterial exopolysaccharides: a perception. Journal of Basic Microbiology. 2007; 47: 103-117.

27. De Philippis R, Vincenzini M. Exocellular polysaccharides from cyanobacteria and their possible applications. FEMS Microbiology Review. 1998; 22: 151-175.

Article Metrics

207 Absract views 226 PDF Downloads 433 Total views

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required