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Volume: 3, Issue: 6, Nov-Dec, 2015
Identification of diazotrophic nostocalean cyanobacteria of north eastern region of India and evaluation for nitrogenase activity and extracellular ammonium excretion
Gunapati Oinam, Wangkhem Indira, O. Avijeet Singh, Th. Indrama, K. Ojit Singh, Laxmipriya Koijam, Chungkham Silvia, A. Subhalaxmi Sharma, Romi Khangembam, Minerva Shamjetshabam, A. Thadoi, K. Sarabati
In this study, morphological and microscopic observations were performed along with growth measurements, evaluation of extracellular ammonium and nitrogenase activity. A total of 62 diazotrophic nostocalean cyanobacteria belonging to 12 genera viz- Scytonema (2), Anabaena (22), Nostoc (17), Cylindrospermum (2), Anabaenopsis (1), Dichothrix (1), Tolypothrix (2), Calothrix (6), Aulosira (4), Microchaete (3), Wollea (1) and Rivularia (1) which were originally isolated from different niches of north east India were procured from National fresh water cyanobacterial and microalgal repository of DBT-IBSD, Imphal, Manipur, India. Among the different nostocalean cyanobacteria encountered from the various habitats, Anabaena sp. BTA650, an isolate from lithophytes under running water, Moreh, Chandel, Manipur exhibited highest nitrogenase activity of 111.08±13.26 nmole C2H4 µg-1 Chl-a h-1 can be a potential candidate for biofertilizer formulation particularly for terraced hill rice culture condition.
Keywords: Diazotrophs, cyanobacteria, nitrogenase activity, extracellular ammonium, repository, biofertilizer.