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Volume: 3, Issue: 5, Sep-Oct, 2015
DOI: 10.7324/JABB.2015.3509

Research Article

An insight into wheat haploid production using wheat x maize wide hybridization

Anjanabha Bhattacharya, Bhavesh Palan, Bharat Char


The present study was carried out with six wheat and four maize genotypes from Mahyco Research Centre, Dawalwadi, Jalna, MH to develop wheat double haploid lines by wheat x maize crossing system. The following steps were followed while conducting the experiment: emasculation of wheat spikes, crossing or pollination of emasculated spikes with maize pollens, treating pollinated spikes with 100 ppm 2,4-D solution, excision of embryo and subsequent culture on half strength MS media. In total, 296 spikes were emasculated and pollinated with randomly collected maize pollens after 24 hrs. Success was obtained in rescuing a total of 27 embryos from 575 caryopses among two genotypes i.e. MW2 and MW3. Percentage of embryo formation varied in the range of 0-10 per cent owing to non-standard conditions. However, the frequency of embryo formation could be increased by focusing on the most important of all culture conditions i.e. temperature (18-21 °C).

Keywords: Wheat, maize, haploid, wheat x maize cross system, 2, 4-D.


1. Grauda D, Lepse N, Strazdina V, Kokina I, Lapina L, Mikelsone A, Lubinskis L, Rashal I. Obtaining of doubled haploid lines by anther culture method for the Latvian wheat breeding. Agronomy Research (Special Issue III). 2010; 8: 545-552.

2. Lashermes P. Improved anther culture method for obtaining direct regeneration in wheat (Triticum aestivum L.). Journal of Genetics and Breeding. 1992; 46: 99-102.

3. Saeed NA, Chowdhry MA, Khan IA. Induction of callus and organogenesis in bread wheat through anther culture. Pakistan Journal of Agriculture Research. 1994; 15(1): 108-114.

4. Lantos C, Paricsi S, Zofajova A, Weyen J, Pauk J. Isolated microspore culture of wheat (Triticum aestivum L.) with Hungarian cultivars. Acta Biologica Szegediensis. 2006; 50(1-2): 31-35.

5. Liu W, Zheng MY, Konzac CF. Improving green plant production via isolated microspore culture in bread wheat (Triticum aestivum L.). Plant Cell Report. 2002; 20: 821-824.

6. Shahinul Islam SM. The effect of colchicine pretreatment on isolated microspore culture of wheat (Triticum aestivum L.). Australian Journal of Crop Science. 2010; 4(9): 660-665.

7. Zheng MY. Microspore culture in wheat (Triticum aestivum)-doubled haploid production via induced embryogenesis. Plant Cell, Tissue and Organ Culture. 2003; 73: 213-230.

8. Campbell AW. Assessment of wheat x maize doubled haploid technology for genetic improvement of New Zealand wheat. A Ph.D. thesis submitted to Lincoln University, Canterbury, New Zealand. 1999.

9. Khan MA, Ahmad J. In vitro wheat haploid embryo production by wheat x maize cross system under different environmental conditions. Pakistan Journal of Agriculture Sciences. 2011; 48(1): 49-53.

10. Niroula RK, Bimb HP, Thapa DB, Sah BP, Nayak S. Production of haploid wheat plants from wheat (Triticum aestivum L.) x maize (Zea mays L.) cross system. Himalayan Journal of Sciences. 2007; 4(6): 65-69.

11. Inagaki M, Mujeeb-Kazi A. Comparison of polyhaploid production frequencies in crosses of hexaploid wheat with maize, pearl millet and sorghum. Breeding Science. 1995; 45: 157-161.

12. Inagaki MN, Tahir M. Production of haploid wheat through intergeneric crosses. Hereditas. 1992; 116: 117-120.

13. Garcia-Llamas C, Ramirez MC, Ballesteros J. Effect of pollinator on haploid production in durum wheat crossed with maize and pearl millet. Plant Breeding. 2004; 123: 201-203.

14. Laurie DA, Bennett MD. Wheat x maize hybridization. Canadian Journal of Genetics and Cytology. 1986; 28: 313-316.

15. Moradi P, Haghnazari A, Bozorgipour R, Sharma B. Development of yellow rust resistant doubled haploid lines of wheat through wheat x maize crosses. International Journal of Plant Production. 2009; 3(3): 77-88.

16. Khan MA, Shaukat S, Ahmad J, Kashif M, Khan AS, Iqbal MZ. Use of intergeneric cross for production of doubled haploid wheat (Triticum aestivum L.). Journal of Science, Technology and Development. 2012; 31(4): 295-300.

17. Niroula RK, Bimb HP. Overview of wheat x maize system of crosses for dihaploid induction in wheat. World Applied Sciences Journal. 2009; 7(8): 1037-1045.

18. Laurie DA, Bennett MD. The production of haploid wheat plants from wheat x maize crosses. Theoretical and Applied Genetics. 1988; 76: 393-397.

19. Ahmad J, Chowdhry MA. The efficiency of chromosome doubling in haploids derived from wheat x maize crossing. Pakistan Journal of Biological Science. 2005; 8(12): 1707-1711.

20. Niroula RK, Thapa DB. Response of Wheat Genotypes to Maize Mediated Polyhaploid Production. American-Eurasian Journal of Agronomy. 2009; 2 (3): 156-161.

21. Torres LE, Bima P, Maich R, Badiali OJJ, Nisi J. Production of haploid plants from ten hybrids of bread wheat (Triticum aestivum L.) through wide hybridization with maize (Zea mays L.). Agriscientia. 2010; Vol. XXVII (2): 79-85.

How to cite this article:
Anjanabha Bhattacharya, Bhavesh Palan, Bharat Char. An insight into wheat haploid production using wheat x maize wide hybridization. J App Biol Biotech. 2015; 3 (05): 044-047. DOI: 10.7324/JABB.2015.3509



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