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Volume: 3, Issue: 5, Sep-Oct, 2015
DOI: 10.7324/JABB.2015.3508

Research Article

A Chromatography based interaction analysis of two important biomolecules: neurotoxic Amyloid beta oligomers and human blood plasma proteins

Anuj Kumer Das

Abstract

A Size exclusion Chromatographic analysis was carried out to study the co-elution pattern as well as the molecular interaction between neurotoxic Amyloid beta oligomers and human blood plasma proteins. Four different types of Chromatographic columns like Sephacryl S-100 HR, Superose 12HR 10/30, Superdex 75 HR 10/300 and Superdex 200 HR10/300 used for analysis and among these columns, Superdex 200 HR 10/300 shown to be the most convenient column with better resolution where 20 mM sodium phosphate, pH 7.4; 150 mM sodium chloride proved as best working buffer at 0.5 ml/min flow rate. Co-elution study of mixture of amyloid β oligomers and human plasma serum showed weak molecular interactions with blood plasma proteins but no strong interaction could be observed.

Keywords: Size exclusion chromatography, molecular interaction, Amyloid beta oligomers, human blood plasma proteins.

References

How to cite this article:
Anuj Kumer Das. A Chromatography based interaction analysis of two important biomolecules: neurotoxic Amyloid beta oligomers and human blood plasma proteins. J App Biol Biotech. 2015; 3 (05): 040-043. DOI: 10.7324/JABB.2015.3508

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