Home > Past Issue

Volume: 3, Issue: 4, July-August, 2015
DOI: 10.7324/JABB.2015.3411

Research Article

Asparagus racemosus extract increases the life span in Drosophila melanogaster

K. V. Kiran Kumar, K. S. Prasanna, J. S. Ashadevi

Abstract

Free radicals and oxidative stress are recognized as important factors in the biology of aging as well as in many age-related diseases. Dietary antioxidant plays a potential role in the reduction of age-related diseases. Many medicinal plants are rich source of antioxidants and promote longevity. Drosophila is a suitable animal model to study ageing and oxidative study. This study evaluated the antioxidant property of Asparagus racemosus on longevity and G6PD activity in Drosophila melanogaster. The analyses were carried out in stress and non stress conditions. Longevity increased almost by 29% at low concentration and 41% at high concentration in extract supplemented flies. G6PD level was significantly increased in extract supplemented larval stages than adults. The antioxidant property of Asparagus racemosus increases the larval G6PD activity thus; it extends the lifespan in adults.

Keywords: Asparagus racemosus, antioxidants, longevity, oxidative stress, Acrylamide, G6PD, Drosphila melanogaster.

References

1. Muller F.L, Lustgarten M.S, Jang Y, Richardson A, Van Remmen.H. Trends in oxidative aging theories. Free RadicBiol Med. 2007; 43:477-503.

2. Perez V.I, Bokov A, Remmen H.V, Mele J, Ran Q, Ikeno Y, Richardson A. Is the oxidative stress theory of aging dead? Biochem Biophys Acta. 2009; 1790:1005-1014.

3. Harshman L.G. Genetic research on aging and longevity of Drosophila melanogaster. Population and Development Review. 2003; 29S: 99-126.

4. Strickberger M.W. Experiments in Genetics with Drosophila. John Wiley and Sons, Inc. 1962. New York.

5. Bourg, E.L. Oxidative stress, aging and longevity in drosophila melanogaster. FEBS Letters. 2011; 498: 183-186.

6. Parisa H, Nargues, Sanaz, V.G, Azadeh M, Gholamreza D, Mohammad A. In vivo antioxidant potential of Teucriumpolium, as compared to a-tocopherol. Acta Pharm. 2007; 57:123-129.

7. Jafari M, Zarban A, Pham S, Wang T. Rosa damascene Decreased Mortality in Adult Drosophila. Journal Of Medicinal Food. 2008; 11: 9-13.

8. Cheng P, Zuo Y, Kwan K M, Liang Y, Ma K.Y, Chan HYE, Huang Y, Yu H, Chen Z. Blueberry extract prolongs life span of Drosophila melanogaster. Experimental Gerentology. 2012; 47: 170-178.

9. Altun D, Ayar A, Uysal H, Kara AA, Unal EL. Extended longevity of Drosophila melanogaster by water and ethanol extracts of Stachyslavandulifolia. Pharm Biol. 2010; 48:1291-1296.

10. Chandrashekara K.T, Shakarad.M.N. Aloe veraorresveratrol supplementation in larval diet delaysadult aging in the fruit fly, Drosophila melanogaster. J Gerontol A BiolSci Med Sci. 2011; 66: 965-971.

11. Lee K.S, Lee B.S, Semnani S, Avanesian A, Um C.Y, Jeon H.J, Seong K.M, Yu K, Min K.J, Jafari M. Curcuminextends life span, improves health span, and modulates the expression of age- (associated aging genes in Drosophila melanogaster. Rejuvenation Res. 2010; 13:561-570.

12. Pant D, Mansi Dave C, Anand K,Tiwari. Wheatgrass (Triticumaestivum L.) Supplementation Promotes Longevity in Drosophila melanogaster. Annals of plant sciences. 2013; 02:49-54.

13. Peng C, Zuo Y, Kwan K.M, Liang Y, Ma K.Y, Chan H.Y,Huang Y, Yu H, Chen Z.Y. Blueberry extract prolongs life span of Drosophila melanogaster. Exp Gerontol. 2012; 47:170-178.

14. Sujata, Mohanty, Rawal S, Singh P, Gupta A. Curcumin longa and Emblica officinalis increase lifespan in Drosophila melanogaster. DrosInfServ. 2011; 94: 122-125.

15. Bahadorani S, Hilliker A.J. Cocoa confers life span extension in Drosophila melanogaster. Nutrition Research. 2008; 28: 377-382.

16. Rege N.N, Thatte U.M, Dahanukar S.A. Adaptogenic properties of six rasayana herbs used in Ayurvedic medicine. Phytother Res. 1999; 13:275-91.

17. Pandey S.K, Sahay A, Pandey R.S, Tripathi Y.B. Effect of Asparagus racemosus rhizome (Shatavari) on mammary gland and genital organs of pregnant rat. Phytotherapy Research. 2005; 19:721-724.

18. Sharma P.V, Charaka. S. Chaukhambhaorientalis. Varanasi India. 2001; 7-14.

19. Hossain M.I, Sharmin F.A, Akhter S, Bhuiyan M.A, Shahriar M. Investigation of Current cytotoxicity and in-vitro antioxidant activity of Asparagus racemosus root extract. International Pharmaceutical Journal. 2012; 1:250-257.

20. Kamat, J.P, Boloor K.K, Devasagayam T.P Venkata-chalam S.R. Antioxidant properties of Asparagus racemosus against damage induced by gamma-radiation in rat liver mitochondria. Journal Ethnopharmacology. 2000; 71: 425-435.

21. Dohare S, Shuaib M, Naquvi K.J. In-vitro antioxidant activity of Asparagus racemosus roots. IJBR. 2011; 24:228‐235.

22. Chan EWC, Lim YY, Omar M. Antioxidant and antibacterial activity of leaves of Etlingera species (Zingiberaceae) in peninsular Malaysia. Food chemistry. 2007; 104: 1586 -1593.

23. Yildirim A, Mavi A, Kara A.A. Determination of Antioxidant and Antimicrobial Activities of Rumexcrispus L.Extracts. Journal of Agricultural and Food Chemistry. 2001; 49:4083-4089.

24. Demiray S, Pintado M.E, Castro PML. Evaluation of Phenolic Profiles and Antioxidant activities of Turkish Medicinal Plants: Tilia Argentea, Crataegi Folium Leaves and Polygonum Bistorta Roots. World Academy of Science, Engineering and Technology. 2009; 54:312 \— 317.

25. Wang S.Y, Jiao H. Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry. Journal of Agricultural and Food Chemistry. 2000; 48:5672-5676.

26. Makkar, H.P.S, Dawra R.K, Singh. B. Determination of both tannin and protein in a tannin- protein complex. J AgricFoodChem. 1998; 523-525.

27. Yoon J.S, Gagen K.P, Zhu D. Longevity of 68 Species of Drosophila. The Ohio Journal of Science.1990; 90:16-32.

28. Prasad S.N, Muralidhara. Evidence of acrylamide induced oxidative stress and neurotoxicity in Drosophila melanogaster- its amelioration with spice active enrichment: relevance to neuropathy. Neurotoxicology. 2012; 33:1254-64.

29. Kumar S, Thomas A, Pillai M.K.K. Involvement of mono-oxygenases as a major mechanism of deltamethrin resistance in larvae of three species of mosquitoes. Indian J ExpBiol. 1991; 29: 379-84.

30. Meydani. M. Nutrition interventions in aging and age-associated diseases. Ann NY Acad Sci. 2001; 928:226-35.

31. Gupta A.K, Tandon N. Reviews on Indian Medicinal Plants.Vol. 3. New Delhi: Indian Council of Medical Research, 2004.

32. Bao, Y., Fenwick, R.G. Phytochemicals in Health and Disease. Marcel Dekker Inc., Basel, New York, pp. 2004; 331-342.

33. Joseph JA, Shukitt-Hale B, Lau FC. Fruit polyphenols and their effects on neuronal signaling and behavior in senescence. Ann N Y AcadSci. 2007; 1100:470\—485.

34. Liu R.H. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr. 2004; 134:3479S-3485S.

35. Shahidi F, Janitha P.K, Wanasundara P.D. Phenolic antioxidants. Critical Reviews of Food Science and Nutrition. 1992; 32:67-103.

36. Havsteen B. Flavonoids a class of natural products of high pharmacological potency. Biochem Pharmacol. 1983; 30:1141-1148.

37. Lee K.P, Simpson S.J, Clissold F.J, Brooks R, Ballard JW, Taylor PW, Soran N, Raubenheimer D. Lifespan and reproduction in Drosophila: New insights from nutritional geometry. ProcNatlAcadSci USA. 2008; 105:2498-2503.

38. Skorupa D.A, Dervisefendic A, Zwiener J, Pletcher S.D. Dietary composition specifies consumption, obesity, and lifespan in Drosophila melanogaster. Aging Cell. 2008; 7:478-490.

39. Papatheodorou I, Petrovs R. and Thornton J.M. Comparison of the mammalian insulin signalling pathway to invertebrates in the context of FOXO-mediated ageing. Bioinformatics Advance Access. 2014; 1-5.

40. Brack C, Bechter-Thuring E, Labuhn M. N-acetylcysteine slows down aging increases the life span of Drosophila melanogaster. Cell MolLife Sci. 1997; 53: 960-966.

41. Driver C, Georgeou A. Variable effects of vitamin E on Drosophila longevity. Biogerontology. 2003; 4:91-95.

42. Sun X, Seeberger J, Alberico T, Wang C, Wheeler C.T, Schauss A.G, Zou S. Açai Palm Fruit (Euterpeoleracea Mart.) pulp improves survival of flies on a high fat diet. Exp Gerontol.2010; 45: 243\—25

43. Hsin-Ping Liu, Rong-Fu Chang, Yih-Shyuan Wu,Wei-Yong Lin, and Fuu-Jen Tsai. The Yang-Tonifying Herbal Medicine Cynomorium songaricum Extends Lifespan and Delays Aging in Drosophila . Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 735481, pp 1-11

44. Aigaki T. and Ohba S. Effect of mating status on Drosophila virilis lifespan. Exp.Rerontol.1984; 19:267-278.

45. Cordts, R. and Partridge, L. Courtship reduces longevity of male Drosophila melanogaster. Animal Behavior. 1996; 52: 269-278.

46. Partridge.L, Ewing.E, Chandler. A. Male size and mating success in Drosophila melanogaster. The role of female and male behaviour. Anim Behav. 1987; 35:555-562.

47. Bahadorani S, Bahadorani P, Phillips JP, Hilliker AJ. The effects of vitamin supplementation on Drosophila life span under normoxia and under oxidative stress. J. Gerontol. A Biol. Sci. Med. Sci. 2008; 63, 35-42.

48. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010; 4:118-126.

49. Sohal R.S, Mockett R.J, Orr W.C. Mechanisms of aging: an appraisal of the oxidative stress hypothesis. Free Rad Biol Med. 2002; 33: 575-586.

50. Leopold J.A, Zhang Y.Y, Scribner A.W, Stanton R.C, Loscalzo J. Glucose-6-Phosphate Dehydrogenase Overexpression Decreases Endothelial Cell OxidantStress and Increases Bioavailable Nitric Oxide. Arterioscler Thromb Vasc Biol. 2003; 23:411-417.

51. Meister. A. Glutathione metabolism and its selective modification. J BiolChem.1988; 263:17205-17208.

52. Fridovich I. Superoxide anion radical (O2-), superoxide dismutases, and related matters. J. BiolChem .1997; 272: 18515-18517.

53. Kirkman H.N, Rolfo M, Ferraris A.M, Gaetani G.F. Mechanisms of protection of catalase by NADPH Kinetics and stoichiometry. Journal of Biological Chemistry. 1999; 274: 13908-13914.

54. Geer B.W, Lindel, D.L,Lindell O.M. Relationship of the oxidative pentose shunt pathway to lipid synthesis in Drosophila melanogaster. Biochem Genetics. 1979; 17: 881-895.

55. Geer BW, Laurie-ahlberg.C.C. Genetic variation in dietary sucrose modulation of enzyme activities in Drosophila melanogaster. Genet. Res. Camb. 1984; 43: 307-321.

56. L S Luckinbill, V Riha, S Rhine and T A Grudzien. 1990 The role of glucose-6-phosphate dehydrogenase in the evolution of longevity in Drosophila melanogaster.

57. Legan S.K,Rebrin I, Mockett R.J, Radyuk S.N, Klichko V.I, Orr W.C, Sohal R.S. Over expression of Glucose-6-phosphate Dehydrogenase extends the life span of Drosophila melanogaster. Journal of Biological Chemistry.2008; 283:32492-32499.

58. Bochdanovits Z. and Jong G. D. Experimental evolution in drosophila melanogaster: interaction of Temperature and food quality selection regimes. Evolution. 2003; 57(8), pp. 1829-1836.

How to cite this article:
Kiran Kumar K.V., Prasanna K.S., Ashadevi J.S. Asparagus racemosus extract increases the life span in Drosophila melanogaster. J App Biol Biotech. 2015; 3 (04): 049-055.

ARTICLE TOOLS

ARTICLE STATISTICS

  • Viewed: 146
  • Downloaded: 137

  Search by Author


      

  Search in Pubmed


   Search in Google Scholar