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Volume: 3, Issue: 4, July-August, 2015
DOI: 10.7324/JABB.2015.3408

Research Article

Effect of external pH on cyanobacterial phycobiliproteins production and ammonium excretion

Ojit Singh Keithellakpam1, Tiwari Onkar Nath1, Avijeet Singh Oinam1, Indrama Thingujam1, Gunapati Oinam1, Sharma Gauri Dutt2

  Author Affiliations


Abstract

In the present study, cyanobacterial strains isolated from Loktak Lake were investigated for their possibility of increasing the content of phycobiliproteins and ammonium excretion under different levels of pH. At pH 5.0, maximum amount of PE was observed in Nostoc sp. BTA-61 (111.10±9.43 µgmg-1). Maximum PC was expressed in Phormidium sp. BTA-1048 (155.17±10.15 µgmg-1). APC content was observed maximum in Nostoc commune BTA-67 (81.45±7.68 µgmg-1). At pH 6.0, high PE was observed in Nostoc sp. BTA-61 (125.11±11.60 µgmg-1). PC was maximum in Phormidium sp. BTA-1048 (168.15±9.58 µgmg-1). APC was highest in Nostoc commune BTA-67 (91.41±2.88 µgmg-1). Regarding at pH 7.0, PE was found in high quantity in Nostoc sp. BTA-61 (127.91±14.91 µgmg-1). PC was maximum in Nostoc commune BTA-67 (128.52±5.66 µgmg-1). APC was highest in Nostoc commune BTA-67 (96.40±3.52 µgmg-1). pH 8.0 showed high PE content in Nostoc sp. BTA-61 (115.75±6.27 µgmg-1). PC was highest in Phormidium sp. BTA-1048 (160.09±10.56 µgmg-1). APC was maximum in Nostoc commune BTA-67 (84.15±5.30 µgmg-1). pH 9.0 showed maximum PE content in Nostoc sp. BTA-61 (95.39±4.87 µgmg-1). PC was high in Phormidium sp. BTA-1048 (148.13±9.20 µgmg-1). APC was highest in Nostoc commune BTA-67 (77.43±5.78 µgmg-1). PE, PC and APC concentration was significantly higher (LSD test, p<0.05) in pH 7.0 when compared with the other pH 5.0, 6.0, 8.0 and 9.0. In this study, maximum ammonium excretion was observed in Nostoc muscorum BTA-950 in all the different pH and minimum in Anabaena sp. BTA-964. The findings indicates that these cyanobacterial strains may be proved as promising microorganism for the phycobiliproteins production and ammonium excretion which could be exploited biotechnologically for the benefit of commercial applications.

Keywords:

Cyanobacteria, Loktak Lake, pH, Phycobiliproteins, Ammonium excretion.



Citation: Ojit S.K., Tiwari O.N., Avijeet S.O, Indrama Th., Gunapati O., Sharma G.D. Effect of external pH on cyanobacterial phycobiliproteins production and ammonium excretion. J App Biol Biotech. 2015; 3 (04): 038-042.


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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