Home >Archive

Volume: 3, Issue: 4, July-August, 2015
DOI: 10.7324/JABB.2015.3407

Research Article

Visible Diode Laser Enhancement of Exotic DNA Uptake by Fowl Sperm

Essam A. El-Gendy1, Mona M. Abdelaziz2, Mohamed M. Abdelfattah2, Mohamed S. Salama3, YhiaM. Badr2

  Author Affiliations


Abstract

An experiment was conducted to assess the use of low power laser irradiation and lipofectin to enhance fowl sperm uptake of exogenous DNA. Semen samples of 10 roosters were collected and pooled. The pooled sample was diluted with a semen extender and then divided into 6 aliquots for 6 different treatments. The treatments included different combinations of the inclusion of exogenous DNA (bacterial plasmid pUC18), the exposure to low power laser irradiation and the transfection with lipofectin (5%). Laser irradiation was by using visible diode laser (650 nm) at energy dose of 4 J/cm2. The recognition of the plasmid DNA in the sperm was by using two specific oligonucleotides (forward and reverse) to prime a 420-bp fragment on the pMB1 rep of the plasmid. The results indicated that low power laser irradiation enhanced the sperm uptake of the plasmid DNA. Also, lipofectin enhanced the introduction of the plasmid DNAinto the sperm, whether the semen was laser irradiated or not.

Keywords:

Fowl sperm, laser irradiation, lipofectin, exotic DNA.



Citation: El-Gendy E.A., Abdelaziz M.M, Abdelfattah M.M, Salama M.S, Badr Y.M. Visible Diode Laser Enhancement of Exotic DNA Uptake by Fowl Sperm. J App Biol Biotech. 2015: 3 (04): 032-037.


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Lillico SG, McGrew MJ, Sherman A, Sang HM. Transgenic chickens as bioreactors for protein-based drugs. Drug Discovery Today. 2005; 10: 191-196.

2. Smith K, Spadafora C. Sperm-mediated gene transfer: applications and implications. BioEssays: news and reviews in molecular, cellular and developmental biology. 2005; 27: 551-562.

3. Lavitrano M, Busnelli M, Cerrito MG, Giovannoni R, Manzini S, Vargiolu A. Sperm-mediated gene transfer. Reproduction, Fertility and Development. 2006; 18: 19–23.

4. El-Gendy EA, Gad AY, Mostageer A. Sperm-mediated gene transfer in poultry 1. The relationship with cock sperm viability. Arab Journal of Biotechnology. 2007; 10: 1-12.

5. Zani M, Lavitrano M, French D, Lulli V, Maione B, Sperandio S, Spadafora C. The Mechanism of Binding of Exogenous DNA to Sperm Cells: Factors Controlling the DNA Uptake. Experimental Cell Research. 1995; 217: 57-64.

6. Gandolfi F. Sperm-mediated transgenesis. Theriogenology. 2000; 53: 127-137.

7. Francolini M, Lavitrano M, Lamia CL, French D, Frati L, Cotelli F, Spadafora C. Evidence for nuclear internalization of exogenous DNA into mammalian sperm cells. Molecular Reproduction and Development. 1993; 34: 133-139.

8. AnzarM,Buhr MM. Spontaneous uptake of exogenous DNA by bull spermatozoa. Theriogenology.2006;65:683-690.

9. Lavitrano M, French D, Zani M, Frati L, Spadafora C. The interaction between exogenous DNA and sperm cells. Molecular Reproduction and Development. 1992; 31: 161-169.

10. Camaioni A, Russo MA, Odorisio T, Gandolfi F, Fazio VM, Siracusa G. Uptake of exogenous DNA by mammalian spermatozoa: specific localization of DNA on sperm heads. Journal of Reproduction and Fertility. 1992; 96: 203-212.

11. Nakanishi A, Iritani A. Gene transfer in the chicken by sperm-mediated methods. Molecular Reproduction and Development. 1993; 36: 258-261.

12. El-Gendy EA, Ahmed MM, El-Tantawy SM. Development of transgenic chickens by sperm-mediated gene transfer. African Journal of Biotechnology. 2013; 12: 2755-2763.

13. Karu TI. Molecular mechanism of the therapeutic effect of low-intensity laser radiation. Laser in Life Sci. 1988; 2: 53–74.

14. Mester E, Mester AF, Mester A. The biomedical effects of laser application. Lasers in Surgery and Medicine. 1985; 5: 31-39.

15. Tafur J, Mills PJ. Low-intensity light therapy: exploring the role of redox mechanisms. Photomedicine and Laser Surgery. 2008; 26: 323-328.

16. Karu TI. Primary and secondary mechanisms of action of visible to near-IR radiation on cells. Journal of Photochemistry and Photobiology B: Biology. 1999; 49: 1-17.

17. Wong-Riley MT, Bai X, Buchmann E, Whelan HT. Light-emitting diode treatment reverses the effect of TTX on cytochrome oxidase in neurons. NeuroReport. 2001; 12: 3033-3037.

18. Grossman N, Schneid N, Reuveni H, Halevy S, Lubart R. 780 nm low power diode laser irradiation stimulates proliferation of keratinocyte cultures: involvement of reactive oxygen species. Lasers in Surgery and Medicine. 1998; 22: 212-218.

19. Karu TI. Mitochondrial signaling in mammalian cells activated by red and near-IR radiation. Journal of Photochemistry and Photobiology. 2008; 84: 1091-1099.

20. Gao X, Xing D. Molecular mechanisms of cell proliferation induced by low power laser irradiation. Journal of Biomedical Science. 2009; 16: 4.

21. Yakimenko I, Besulin V, Testik A. The effects of low intensity red laser irradiation on hatching eggs in chicken and quail. International Journal of Poultry Science. 2002; 1: 6-8.

22. El-Gendy EA. A model for the genetic employment of chickens local to warm climate 1. Crossing with a fast growing strain and growth patterns of the crossbreds. International Journal of Poultry Science.2009;8: 299-306.

23. Lake PE. Fowl semen as collected by the massage method. The Journal of Agricultural Science. 1957; 49: 120-126.

24. Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene. 1985;33:103-119.

25. Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielsen M. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proceedings of the National Academy of Sciences. 1987; 84: 7413-7417.

26. Sexton TJ. A new poultry semen extender 1. effect of extension on the fertility of chicken semen. Poultry Science. 1977; 56: 1443-1446.

27. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual. NY, USA: Cold Spring Harbor Laboratory Press, Cold Spring Harbor; 1989.

28. Walker S, Sofia MJ, Axelrod HR. Chemistry and cellular aspects of cationic facial amphiphiles. Advanced Drug Delivery Reviews. 1998; 30: 61-71.

29. Leventis R, Silvius JR. Interactions of mammalian cells with lipid dispersions containing novel metabolizable cationic amphiphiles. Biochimica et Biophysica Acta (BBA) – Biomembranes. 1990; 1023: 124-132.

30. Behr JP. Gene transfer with synthetic cationic amphiphiles: prospects for gene therapy. Bioconjugate Chemistry.1994;5: 382-389.

31. Axelrod HR, Sofia MJ, Walker S. Chemistry and cellular aspects of cationic facial amphiphiles. Advanced Drug Delivery Reviews. 1998; 30: 61-71.

32. Lanes CF, Sampaio LA, Marins LF. Evaluation of DNase activity in seminal plasma and uptake of exogenous DNA by spermatozoa of the Brazilian flounder Paralichthys orbignyanus. Theriogenology. 2009; 71: 525-533.

33. Carballada R,Esponda P. Regulation of foreign DNA uptake by mouse spermatozoa. Experimental Cell Research.2001;262: 104-113.

34. Sato F, Soh T, Hattori MA, Fujihara N. Evaluation of deoxyribonuclease activity in seminal plasma of ejaculated chicken semen. Asian Journal of Andrology. 2003; 5:213-216.

35. Nechaevsky YV, Ivanov VA. DNase II in spermatozoa of the loach misgurnus fossilis L. Biochemistry. 1999; 64: 488-493.

36. Collares T, Campos VF, DeLeon PM, Cavalcanti PV, Amaral MG, Dellagostin OA,Deschamps JC,Seixas FK. Transgene transmission in chickens by sperm-mediated gene transfer after seminal plasma removal and exogenous DNA treated with dimethylsulfoxide or N,N-dimethylacetamide. Journalof Bioscience. 2011;36:613-620

37. Lubart R, Friedmann H, Levinshal T, Lavie R, Breitbart H. Effect of light on calcium transport in bull sperm cells. Journal of Photochemistry and Photobiology B: Biology. 1992; 15: 337-341.

38. Lubart R, Friedmann H, Sinykov M, Cohen N, Breitbart H. Changes in calcium transport in mammalian sperm mitochondria and plasma membranes caused by 780 nm irradiation. Lasers in Surgery and Medicine. 1997; 21: 493-499.

39. Iaffaldano N, Rosato MP, Paventi G, Pizzuto R, Gambacorta M, Manchisi A and Passarella S. The irradiation of rabbit sperm cells with He–Ne laser prevents their in vitro liquid storage dependent damage. Animal Reproduction Science.2010;119: 123-129.

Article Metrics

Similar Articles