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Volume: 3, Issue: 4, July-August, 2015
Modulation of phycobiliprotein production in Nostoc muscorum through culture manipulation
Onkar Nath Tiwari, Wangkhem Indira Devi, Chungkham Silvia, Angom Thadoi Devi, Gunapati Oinam, Oinam Avijeet Singh, Keithellakpam Ojit Singh, Thingujam Indrama, Aribam Subhalaxmi Sharma, Romi Khangem
Nostoc sp. BTA87 was procured from National fresh water cyanobacterial and microalgal repository of IBSD-DBT, Imphal, Manipur, India. This was identified as Nostoc muscorum based upon morphological characteristics and 16S rRNA gene sequence analysis. Cultivation of organism under two cultural conditions indicated that phycobiliproteins enhanced under dialyzed conditions in comparison to non-dialyzed conditions. Red light was more effective to green and blue light for enhanced production of phycocyanin and allophycocyanin whereas phycoerythrin was produced more under green light in dialyzed condition. The optimum pH for maximum production of all the three components of phycobiliproteins was 8.0. The results clearly indicated that the production of phycobiliproteins can be enhanced by cultivating cyanobacterium in dialyzed conditions at pH-8.0 under optimum light quality and Nostoc muscorum can be a potential candidate for the commercial production of phycobiliproteins.
Keywords: Dialyzed culture, pH, light quality, Nostoc muscorum, phycobiliproteins.