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Volume: 1, Issue: 2, July- Aug, 2013
DOI: 10.7324/JABB.2013.1204

Research Article

Effect of Wild and Mutant Strain of Lasiodiplodia Pseudotheobromae Mass Produced on Rice Bran as a Potential Bioherbicide Agents for Weeds under Solid state Fermentation

Adetunji C. O., Oloke J.K.

Abstract

Local available agro-industrial waste from rice bran was evaluated in the laboratory for use as substrate for mass culturing of Lasiodiplodia pseudotheobromae. Boiling water and Anona senegalensis extracts were added to the substrate under solid state fermentation (SSF). The effects of lengthy exposure to Ultraviolet (UV) radiations (1h 30min,1hours, 30 minutes) on the conidia production were assessed after 14days with 12h photoperiod at 25-30oC. The laboratory studies showed that rice bran with boiling water in nylon bag inoculated with the mutant strain of Lasiodiplodia pseudotheobromae exposed for 1hour 30 minutes had the highest number of conidia with 3.80 × 109 cfu.g-1 followed by Lasiodiplodia pseudotheobromae exposed to 30 minutes U.V. radiation with conidia number of 2.08 × 109 cfu.g-1 while the wild type had a conidia of 1.66 × 109 cfu.g-1 after 14days with 12hours photoperiod at 25-30ºC. Therefore, agro-industrial wastes from rice bran could be suitable for mass production of bioformulated products of Lasiodiplodia pseudotheobromae which are used for the control of weeds in Nigeria.

Keywords: Agro-industrial wastes, conidia production, solid state fermentation, Lasiodiplodia pseudotheobromae, Rice bran.

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