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Volume: 3, Issue: 3, May-June, 2015
DOI: 10.7324/JABB.2015.3308

Research Article

Genotoxic effect of distillery effluent on root tip cells of Allium sativum L.

K. L. Hemavathi, N. K. Hemanth Kumar, Shobha Jagannath

Abstract

In the current study an attempt has been made to evaluate the cytotoxic efficacy of distillery effluent on somatic cells of Allium sativum. The garlic bulbs were treated with different concentrations of effluent viz. 2.5, 5, 7.5 and 10% at room temperature. The percentage mitotic index was found to be decreased significantly as the concentration of the effluent increased, except in 2.5% concentration, where the mitotic index is higher than control. The chromosomal abnormalities were found to be increased as the effluent concentration increased when compared to control. The observed chromosomal aberrations were laggards, fragmented chromosome, fragmented anaphase and metaphase, chromosomal bridges, micronuclei, lobed nucleus, scattered chromosome, disoriented anaphase, Polyploidy cells. According to present findings 2.5% concentration of the effluent was found to enhance the rate of cell division when compare to control on the contrary higher of the effluent showed negative effects on mitotic division in somatic cells of Garlic.

Keywords: Distillery, mitotic index, garlic, chromosome.

References

1. Saxena HM, Environmental Studies. Rawat Publications, New Delhi: 2006: pp.94-97.

2. Mishra K, Cytotoxic Effect Of Distillery Waste water on Allium cepa L..Bulletin Environmental Contamination Toxicology. 1993: 50: 199-204.

3. Al-Rashed MF, Sherif MM. Water Resources in the GCC Countries: an overview. Water Resource Management. 2000; 14(1):59-73.

4. Singh PK, Sharma KP. Biomethanated distillery waste utilization by wetland plant: A New Approach. World Journal of Agricultural Science. 2012: 8(1): 96-103.

5. Kovacs A, Mesgaros M. Water as Irrigation; Water polluted with various compounds. TISCIA, 1986;21 : 25-29.

6. Gori R, Ferrini F, Nicese FP, Lubello C. Effect of Reclaimed waste water on the growth and nutrient content of three landscape shrubs. Journal of Environmental Horticulture. 2000;18 (2) : 108-114.

7. Egbenni POU, Okolie PN, Adejuyitan OE, Sobande AO, Akinyemi O. Effect of Industrial effluents on the growth and anatomical structure of Abelmoschus esculentus (Okra). African Journal of Biotechnology. 2009;8 (14) : 3251-3260.

8. Ray M. Barman. Effects of Industrial Waste water on Root Meristem Cells of Allium sativum L. Phytomorphology. 1987; 37:359-364.

9. Shamim AA, Bahar AS, Khan S. Genotoxic effect of smoke as a pollutant on Cestrum diurnum L., Advances in Plant Science. 2002;15:573-577.

10. Mishra V, Pandey SD. Effect of Distillery effluent and Leachates of Industrial Sludge on the germination of Black Gram (Phaseolus mungu). PollutionResesrch. 2002;21:461-467.

11. Dhankar R, Khatri S, Dahiaya JS. Inhibition of Nitrate reductase activity in some crop plants raised with sewage waste water. Journal of Ecophysiological Occupational Health. 2002; 2: 235-242.

12. Singh A, Agarwal SB, Rai JPN, Singh P. 2002. Assessment of Pulp and Paper mill effluent on growth , yield and nutrient quality of Wheat (Triticum aestivum). Journal of Environmental Biology. 2002; 28: 283-288.

13. Subramanian KA, Singal SK, Saxena M, Singhal S. Utilization of liquid biofuels in automotive diesel engines: an Indian perspective. Biomass and Bioenergy. 2005;29 (1) : 65-72.

14. Uppal J. Water utilization and effluent treatment in the Indian alcohol industry \— an overview. In Indo workshop and promoting efficient water in agrobased industries. New Delhi.2004;15-16.

15. Saxena PN, Murthy RC, Gupta SK. Evaluation of Cytogenetic effects of deltamethrin in root meristem cells of Allium sativum L. and Allium cepa L.: A possible mechanism of Chromosome damage. Toxicology and Environmental Chemistry. 2009;91 (3) : 577-594.

16. Earnst WHO, Peterson PJ. 1994. The Role of Biomarkers in Environmental Assessment (4). Terrestrial Plants Exotoxicology. 1994;3:180-190.

17. Hose JE. Large scale Genotoxicity Asssessment in the Marine environment. Environment Health Perspectives,1994;102:29-32.

18. Fiskesjo G. The Allium test as a Standard in Environmental Monitoring. Hereditas, 1985;102(1):99-112.

19. Fiskesjo G. Allium test.In votro toxicity testing protocols. Methods in Molecular Biology. 1995;43:119-127.

20. Bushra A, Abdul FM, Naimat AM, Ahmad M. Clastogenecity of Pentachlorophenol, 2,4-D and Butachlor evaluated by Allium root tip test. Mutation Research. 2002;514:105-113.

21. Srivastava S, Jain R. Effect of distillery spent wash on Cytomorphological behavior of Sugarcane settlings. Journal of Environmental Biology. 2010;3(5):809-812.

22. Gonzalez-Fernandez A, Lopez-Saez JF. Effect of caffeine and adenosine on G2 repair, mitotic delay and chromosome damage. Mutation Research. 1985;149:275-281.

23. Chauhan LK, Saxena PN, Gupta SK. Evaluation of Cytogenetic effects of Isoproturon on the root meristem cells of Allium sativum L. .Biomedical and Environmental Science. 2001;14(3):214-219.

24. Fenech M. The in-vitro micronucleus technique. Mutation Research.2002;45: 81-95.

25. Luo LZ, Werner KM, Gollin SM, Saunders WS. Cigarette smoke induces anaphase bridges and genomic imbalance in normal cells. Mutation Research. 2004; 554: 375 \— 378.

26. Grant WF. Chromosome Aberrations in Plants as a Monitoring System. Environment Health Perspectives. 1978; 27: 37-43.

27. Chakraborty R, Mukherjee AK, Mukherjee A. Evaluation of genotoxicity of Coal fly ash in Allium cepa L. root cells combining comet assay with the Allium test. Environment Moniterring Assessment. 2008;153:351-357.

28. Nielson MH, Rank J. Screening of Toxicity and Genotoxicity in waste water by the use of the Allium test. Hereditas, 1994; 12 :249-254.

29. Gomurgen AN. 2000. Cytological effect of the Herbicide 2, 4-D isooctylesteer 8% on root mitosis of Allium cepa L. Cytologia. 2000; 65:383-388.

30. Patil BC, Bhat BI. A Comparitive study of MH and EMS in the induction of chromosomal aberrations on lateral root meristem in Clitoria ternatia L. Cytologia. 2000; 57: 259-264.

How to cite this article:
Hemavathi K. L., Hemanthkumar N. K. and Shobha Jagannath. Genotoxic effect of distillery effluent on root tip cells of Allium sativum L. J App Biol Biotech. 2015; 3 (03): 038-041.

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