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Volume: 3, Issue: 3, May-June, 2015
Genotoxic effect of distillery effluent on root tip cells of Allium sativum L.
K. L. Hemavathi, N. K. Hemanth Kumar, Shobha Jagannath
In the current study an attempt has been made to evaluate the cytotoxic efficacy of distillery effluent on somatic cells of Allium sativum. The garlic bulbs were treated with different concentrations of effluent viz. 2.5, 5, 7.5 and 10% at room temperature. The percentage mitotic index was found to be decreased significantly as the concentration of the effluent increased, except in 2.5% concentration, where the mitotic index is higher than control. The chromosomal abnormalities were found to be increased as the effluent concentration increased when compared to control. The observed chromosomal aberrations were laggards, fragmented chromosome, fragmented anaphase and metaphase, chromosomal bridges, micronuclei, lobed nucleus, scattered chromosome, disoriented anaphase, Polyploidy cells. According to present findings 2.5% concentration of the effluent was found to enhance the rate of cell division when compare to control on the contrary higher of the effluent showed negative effects on mitotic division in somatic cells of Garlic.
Keywords: Distillery, mitotic index, garlic, chromosome.