Home >Archive

Volume: 3, Issue: 3, May-June, 2015
DOI: 10.7324/JABB.2015.3303

Research Article

Antioxidant effect of Tryptophan on biochemical parameters in the haemolymph and fat body of final instar larvae of silk insect, Bombyx mori

K. P. Priya Bhaskaran, P. U. Bindu, K. Rukhsana, V. P. Akhilesh, E. K. Jisha Krishnan, C. D. Sebastian

  Author Affiliations


Abstract

Ageing is a natural life process whose manifestations are familiar and unambiguous. Oxidative modification of cellular molecules by reactive oxygen species and impaired antioxidant mechanism play unique role in a variety of age-associated degenerations. As a defence, cells have developed antioxidant defence system of a group of enzymes including catalases and peroxidases destroying toxic molecules. The natural antioxidant mechanism of an organism may be insufficient and external dietary administration of anti oxidant compounds play vital role in defence against ageing. In the present study, the antioxidant effects of reducing amino acid tryptophan in the final instar larvae of silkworm, Bombyx mori. The turnover of total protein, amino acid and glucose was evaluated. The total haemolymph protein of treated larvae showed 34-94% increase when compared to normal and the pattern of the changes in the levels of fat body protein was same but with a change of 12 fold. The total content of free amino acids in the haemolymph of normal and treated larvae increased gradually from the period of 0 h to 96 h with a peak value at 96 h and then decreased. The total content of free amino acids in the fat body is much less than that found in the haemolymph. The peak glucose levels in the total larval haemolymph were almost 28 times to that found at the early stage in normal larvae and approximately 12 times in tryptophan treated larvae. The fat body glucose level showed a consistent reduction in the treated larvae.

Keywords:

Bombyx mori, ageing, tryptophan, total protein, free amino acids, glucose.



Citation: Priya Bhaskaran K. P., Bindu P. U., Rukhsana K., Akhilesh V. P., Jisha Krishnan E. K., and Sebastian C. D. Antioxidant effect of Tryptophan on biochemical parameters in the haemolymph and fat body of final instar larvae of silk insect, Bombyx mori. J App Biol Biotech. 2015; 3 (03): 011-015.


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Lean AJM, Counter DGL. Aging Biology and Geriatric Clinical Pharmacology. Pharmacology and Revolution, 2004; 56(2), 163-184.

2. Kolawole AO, Olajuyigbe FM, Ajele JO, Adedire CO. Activity of the antioxidant defense system in a typical bioinsecticide- and synthetic insecticide-treated cowpea storage beetle Callosobrochus maculatus F. (Coleoptera: Chrysomelidae). International Journal of Insect Science, 2014; 6: 99-108.

3. Cheng PC, Wang S, Chen J, Jiao R, Wang L, Li YM, Zuo Y, Liu Y, Lei L, Ma KY, Huang Y, Chen ZY. Biology of ageing and role of dietary antioxidants. Biomedical Research International, 2014; 14: 831-841.

4. Amritha S, Sumisha K, Sebastian CD. Effects of Antioxidant food Supplement on aging in Bombyx mori. International Research Journal of Biological Science, 2014; 3(4): 61-65.

5. Borek C. Antioxidant health effects of aged garlic extract. Journal of Nutrition, 2010; 131:1010-1015.

6. Izabela Sadowska-Bartosz, Grzegorz Bartosz. Effect of Antioxidants Supplementation on Aging and Longevity. Biomedical Research International, 2014; 404680: 17 pp.

7. Reddy KVR, Remadevi OK, Benchamin KV. Impact of the uzifly parasitization on the body growth, silk gland tissue somatic index and haemolymph properties of silkworm, Bombyx mori. Indian Journal of Sericulture, 1994; 39: 113-120.

8. Moosmann B, Behl C. Cytoprotective antioxidant function of tyrosine and tryptophan residues in transmembrane proteins. European Journal of Biochemistry, 2002; 5: 251-264.

9. Chipppendale GM, Kilby BA. Relationship between the proteins of the haemolymph and fat body during development of Pieris brassicae. Journal of Insect Physiology, 1969; 15: 905-926.

10. Whitton PS, Nicholson RA, Bell MF, Strang RHC. Biosynthesis of taurine in the tissue of the locust, Schistocerca americana gregaria and the effect of physiological and toxicological stresses on biosynthesis rate of the amino acids. Insect Biochemistry and .Molecular Biology, 1995; 25: 83-87.

11. Shigematsu H. Synthesis of blood proteins by the fat body in the silkworm Bombyx mori L. Nature, 1958; 182: 880-882.

12. Lazar KV, Mohamed UVK. Amino transferases and glucose levels in developing larvae of the moth, Spodoptera mauritia. Journal of Animal Morphology and Physiology, 1998; 45: 41-43.

13. Lowry OH, Rosenburgh NJ, Farr AL, Randall RJ. Protein measurement with the Folin Phenol reagent Journal of Biochemistry, 1951; 193: 265-275.

14. Lee YP, Takahashi T. An improved colorimetric determination of amino acids with the use of ninhydrin. Analytical Biochemistry, 1966; 14: 71-77.

15. Morgan MRJ. Initial characterization of the gut cellobiase of the African Migratory locust, Locusta migratoria migratorioideas. Insect Biochemistry, 1975; 5: 251-264.

16. Chen PS. Aminoacid and Protein Metabolism. Comprehensive Insect Physiology and Biochemistry and Pharmacology, 1985; 10: 177-218.

17. Florkin M, Jeuniaux C. Haemolymph: Composition. The Physiology of Insecta. II Edition, Academic Press, London, 1974; 5: 255-307.

18. Terra WR, Ferreira C, Blangi AG. Distribution of nutrient reserves during spinning in tissues of the larva of the fly, Rhychosciara americana. Journal of Insect Physiology, 1975; 21: 1301-1301.

19. Tojo S, Betchaku T, Ziccardi VJ, Wyatt GR. Fat body protein granules and storage proteins in the silk moth, Hyalophora cecropia. Journal of Cell Biology, 1978; 78: 823-838.

Article Metrics

Similar Articles

Effect of polyamines on parental and hybrid strains of Bombyx mori
Aparna Yerra, Anitha Mamillapalli