Allelopathic potential of Annona muricata (L.) on physiological and biochemical changes of Vigna radiata (L.) and Eleusine coracana (L.) Gaertn.

Edwina Kannan Leela Palayian   

Open Access   

Published:  Jan 06, 2022


An experiment was conducted to assess the allelopathic potential of leaf extract of Annona muricata (L.) on physiological and biochemical changes of Vigna radiata (L.) and Eleusine coracana (L.) Gaertn. Annona muricata inhibits and stimulates other plants’ growth, a phenomenon called “allelopathic effect.” Crude water extract of the leaves of A. muricata was treated on V. radiata and E. coracana in different concentrations such as 1%, 2%, 3%, 4%, and 5%, respectively. A control crop was treated with distilled water. The morphological and physiological effects on V. radiata and E. coracana were observed and recorded. Data were recorded on seed germination (after 10 days of seed sowing), growth, and biochemical composition (15 days old seedlings). Reduction in germination percentage of V. radiata and E. coracana was recorded using leaf extract of A. muricata. The outcome of this research work showed that the extracts from the leaves of the A. muricata plant inhibit and stimulate the growth and development of the test seedlings. The biochemical constituents of test seedlings, such as chl a, chl b, carotenoid, protein, amino acid, carbohydrate, peroxidase, and catalase were observed. The extracts were absorbed through the roots of these plants, affecting physiological processes such as photosynthetic and transpiratory rates, stomatal closure, and reduced chlorophyll content, resulting in wilting, tissue maceration, and stunted growth. Between the two crops, more allelopathic effect was recorded in E. coracana. These results showed that there are differences in allelopathic inhibition and stimulation of crops.

Keyword:     Allelopathy biochemical physiological germination Annona muricata Vigna radiata Eleusine coracana


Kannan E, Palayian L. Allelopathic potential of Annona muricata (L.) on physiological and biochemical changes of Vigna radiata (L.) and Eleusine coracana (L.) Gaertn. J Appl Biol Biotech, Online First.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1.Mbagwu FN. The allelopathic effects of crude water extracts of Annona muricata on common weed. Int J Nat Appl Sci 2006;2(4):341-4.

2. Oracz K, Bouteau HE, Farrant JM, Cooper K, Belghazi M, Job C, et al. ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation. Plant J 2007;50:452-65.

3. Bais HP, Vepachedu R, Gilroy S, Callaway RM, Vivanco JM. Allelopathy and exotic plant invasion: from molecules and genes to species interaction . Science 2003;301:1377-80.

4. Duke SO, Dayan FE, Rimando AM, Schrader KK, Aliotta G, Oliva A, et al. Chemicals from nature for weed management. Weed Sci 2002;50:138-51.[0138:IPCFNF]2.0.CO;2

5. Duke SO, Dayan FE. Modes of action of phytotoxins from plants. In: Reigosa MJ, Pedrol N, González L (eds.). Allelopathy: A Physiological Process with Ecological Implications, Springer, Amsterdam, The Netherlands, pp 511-36, 2006.

6. He HB, Wang HB, Fang CX, Lin ZH, Yu ZM, Lin WX. Separation of allelopathy from resource competition using rice/barnyard grass mixed-cultures. PLoS One 2012;7:e37201.

7. An Y, Ma Y, Shui J. Switchgrass (Panicum virgatum L.) plants and switchgrass residue reduce the biomass and density of associated weeds. Acta Agric Scand 2013;63:107-13.

8. Khanh T, Chung M, Xuan T, Tawata S. The exploitation of crop allelopathy in sustainable agricultural production. Agron Crop Sci 2005;191:172-84.

9. Scavo A, Restuccia A, Mauromicale G. Allelopathy: principles and basic aspects for agroecosystem control. J Sustain Rev 2018;28:47- 101.

10. Jacob J, Sreekumar KM, Rekha P. Allelopathic effects of leaf leachates of multipurpose trees on vegetables. Allelopathy J 2001;19:507-16.

11. Peng SL, Wen J, Guo QF. Mechanism and active variety of allelochemicals. Acta Bot Sin 2004;46:757-66.

12. Albuquerque MB, Santos RC, Lima LM, Melo Filho PDA, Nogueira RJMC, Câmara CAG, et al. Allelopathy, an alternative tool to improve cropping systems. Rev Agron Sust Dev 2010;31:379-95.

13. Fernandez C, Santonja M, Gros R, Monnier Y, Chomel M, Baldy V, et al. Allelochemicals of Pinus halepensis as drivers of biodiversity in Mediterranean open mosaic habitats during the colonization stage of secondary succession. J Chem Ecol 2013;39:298-311.

14. Coria-Téllez AV, Montalvo-Gónzalez E, Yahia EM, Obledo-Vázquez EN. Annona muricata: a comprehensive review on its traditional medicinal uses. Arab J Chem 2016;5:662-91.

15. Lowery. O.H, Rosenbrough, N.J, Farr. A.L., Randall. R. J. "Protein measurement with the folin phenol reagent". J Biol Chem 1951;193:265-27.

16. Venkateshwarlu G, Ravindra V, Challa P. Mangiferin: an allelopathin from mango (Mangifera indica L.) leaves. Allelopathy J 2001;8(2):221-4.

17. Sahoo UK, Jeeceelee L, Vanlalhriatpuia K, Upadhyaya K, Lalremruati JH. Allellopathic effects of leaf leachate of Mangifera indica L. on initial growth parameters of few home garden food crops. World Appl Sci J 2010;10(12):1438-47.

18. Rice EL. Allelopathy. 2nd edition, Academic Press, Orlando, FL, 1984.

19. Shahena S, Rajan M, Chandran V, Mathew L. Allelopathic effect of Wedelia trilobata L., on the germination and growth of Cicer arietinum, Vigna unguiculata, and Vigna radiata seedlings. J App Biol Biotech 2021;9(2):93-114.

20. Murimwa JC, Rugare JT, Mabasa S, Mandumbu R. Allelopathic effects of aqueous extracts of sorghum (Sorghum bicolor L. Moench) on the early seedling growth of Sesame (Sesamum indicum L.) varieties and selected weeds. Int J Agron 2019;2019:1-12.

21. Mangao AM, Arreola SLB, Gabriel EVS, Salamanez KC. Aqueous extract from leaves of Ludwigia hyssopifolia (G. Don) exell as potential bioherbicide. J Sci Food Agric 2020;100:1185-94.

22. Islam AKMM, Hasan MM, Yeasmin S, Abedin MA, Kader MA, Rashid MH, et al. Bioassay screening of tropical tree sawdust for allelopathic proper ties and their field performance against paddy weeds. Fundam Appl Agric 2019;4(3):906-15.

23. Abu-Romman S, Shatnawi M, Shibli R. Allelopathic effects of spurge (Euphorbia hierosolyminata) on wheat (Triticum durum). AEJAES 2010;7(3):298-302.

24. Arowosgabe S, Wintola OA, Afolayan AJ. Phytochemical constituents and allelopathic effect of Aloe ferox Mill. root extract on tomato. JMPR, 2012;6(11):2094-9.

25. Morikawa CIO, Miyaura R, de Lourdes Tapia y Figueroa M, Rengifo Salgado EL, Fujii Y. Screening of 170 Peruvian plant species for allelopathic activity by using the sandwich method. Weed Biol Manage 2012;12:1-11.

26. Qin B, Perry LG, Broeckling CD, Du J, Stermitz FR, Paschke MW, et al. Phytotoxic allelochemicals from roots and root exudates of leafy spurge (Euphorbia esula L.). Plant Signal Behav 2006;1:323-7.

27. Rudrappa T, Quinn WJ, Stanley-Wall NR, Bais HP. A degradation product of the salicylic acid pathway triggers oxidative stress resulting in down-regulation of Bacillus subtilis biofilm formation on Arabidopsis thaliana roots. Planta 2007;226:283-97.

28. Yu JH, Zhang Y, Niu CX, Li JJ. Effects of two kinds of allelochemicals on photosynthesis and chlorophyll fluorescence parameters of Solanum melongena L. seedlings. Chin J Appl Econ 2006;17:1629-32.

29. Weir TL, Park SW, Vivanco JM. Biochemical and physiological mechanism mediated by allelochemicals. Curr Opin Plant Biol 2004;7:472-9.

30. Wang CM, Chen HT, Li TC, Weng JH, JhanYL, Lin SX. The role of pentacyclic triterpenoids in the allelopathic effects of Alstonia scholaris. J Chem Ecol 2014;40:90-8.

31. Poonpaiboonpipat T, Pangnakoran U, Suvunnamek U, Teerarark M, Charoenying P, Laosinwatanna C. Phytotoxic effect of essential oil from Cymbopogan citratus and its physiological effects of banyard grass. Indian Crop Prod 2003;41:403-7.

32. Batish RD, Setia N, Singh HP, Kohli RK. Phytotoxicity of lemonscented eucalypt oil and its potential use as a bioherbicide. Crop Prot 2004;23:1209-14.

33. Singh HP, Batish DR, Setia N, Kohli RK. Herbicidal activity of volatile essential oils from Eucalyptus citriodora against Parthenium hysterophorus. Ann Appl Biol 2005;146:89-94.

34. Kaur S, Singh HP, Mittal S, Batish DR, Kohli RK. Phytotoxic effects of volatile oil from Artemisia scoparia against weeds and its possible use as a bioherbicide. Ind Crops Prod 2010;32:54-61.

35. Scott P. Physiology and behaviour of plants. John Wiley & Sons Inc., Hoboken, NJ, pp 20-1, 2008.

36. Frabboni T, Tarantino D, Petruzzi F, Disciglio G. Bio-herbicidal effects of oregano and rosemary essential oils on chamomile (Matricaria chamomilla L.) crop in organic farming system. Agronomy 2019;9(9):475.

37. Ojija F, Arnold SEJ, Treydte AC. Bio-herbicide potential of naturalised Desmodium uncinatum crude leaf extract against the invasive plant species Parthenium hysterophorus. Biol Invasions 2019;21:3641-53.

38. Dankov K, Busheva M, Stefanov D, Apostolova EL. Relationship between the degree of carotenoid depletionand function of the photosynthetic apparatus. J Photochem Photobiol B 2009;96:49-56. 39.

39. Siyar. S, Majeed A., Muhammad Z.,. Ali .H., and Inayat. N., "Allelopathic effect of aqueous extracts of three weed species on the growth and leaf chlorophyll content of bread wheat," Acta Ecologica Sinica, vol. 39, no. 1, pp. 63-68, 2019.

40. Sofi PA, Djanaguiraman M, Siddique KHM, Prasad PVV. Reproductive fitness in common bean (Phaseolus vulgaris L.) under drought stress is associated with root length and volume. Indian J Plant Physiol 2018;23(4):796-809.

41. Zeng RS, Luo SM, Shi YH, Shi MB, Tu CY. Physiological and biochemical mechanism of allelopathy of secalonic acid F on higher plants. Agron J 2001;93:72-9.

42. Li ZH, Wang Q, Ruan X, Pan CD, Jiang DA. Phenolics and plant allelopathy. Molecules 2010;15:8933-52.

43. Fang C, Li Y, Li C, Li B, Ren Y, Zheng H, et al. Identification and comparative analysis of microRNAs in barnyardgrass (Echinochloa crus-galli) in response to rice allelopathy. Plant Cell Environ 2015;38:1368-81.

44. Kato-Noguchi H, Ota K. Biological activities of rice allelochemicals momilactone A and B. J Rice Res 2013;1, 108-12.

45. Yang QH, Ye WH, Liao FL, Yin XJ. Effects of allelochemicals on seed germination. Chin J Ecol 2005;24:1459-65.

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