Research Article | Volume: 10, Issue: 1, January, 2022

Biodegradation and detoxification of low-density polyethylene by an indigenous strain Bacillus licheniformis SARR1

Ritu Rani Jitender Rathee Poonam Kumari Nater Pal Singh Anita Rani Santal   

Open Access   

Published:  Jan 07, 2022

DOI: 10.7324/JABB.2021.100102
Abstract

Plastics are synthetic polymers, which are frequently used in daily life for a wide range of purposes. These plastic wastes are accumulated and generate plastic pollution in the environment. It needs many years for complete deterioration in the environmental conditions. Biodegradation is the most promising method to treat plastic pollution, as the microorganisms utilize the low-density polyethylene (LDPE) as a sole source of carbon, and this indicates an innovative approach to manage the problem of plastic waste. The isolate SARR1 was identified as Bacillus licheniformis using the National Center for Biotechnology Information (NCBI) database. The isolate SARR1 showed an LDPE removal rate (K) of 0.069 g day−1 with a half-life of approximately 335.32 days to degrade LDPE strips. The biomass production was 0.98 ± 0.006 gl−1 (Xm) during the incubation of 30 days, and the percentage of crystallinity was significantly decreased from 71.69% to 50.78% due to biodegradation. The esterase and lipase activity of isolate SARR1 was studied using UV visible spectroscopy. The gas chromatography-mass spectrometry analysis confirmed the synthesis of acetone, diazene dimethyl, and carbamimidothioic acid, 1-methylethyl ester with different peak area percentages of 23.38%, 65.58%, and 11.04%, respectively. Seed germination study showed that the compounds formed after biodegradation of LDPE by bacterial strain SARR1 were eco-friendly.


Keyword:     Detoxification LDPE Bacillus licheniformis UV visible spectroscopy GC-MS


Citation:

Rani R, Rathee J, Kumari P, Singh NP, Santal AR. Biodegradation and detoxification of low-density polyethylene by an indigenous strain Bacillus licheniformis. J Appl Biol Biotech. 2022; 10(01):9–21.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Reference

1. Sridharan R, Vetriselvan M, Krishnaswamy VG, Rishin H. Integrated approach in LDPE degradation-an application using Winogradsky column, computational modeling, and pathway prediction. J Hazard Mater 2021;412:125336. https://doi.org/10.1016/j.jhazmat.2021.125336

2. El-Sayed MT, Rabie GH, Hamed EA. Biodegradation of low-density polyethylene (LDPE) using the mixed culture of Aspergillus carbonarius and A. fumigates. Environ Dev Sustainability 2021;23:1- 29. https://doi.org/10.1007/s10668-021-01258-7

3. Dey A, Dhumal CV, Sengupta P, Kumar A, Pramanik NK, Alam T. Challenges and possible solutions to mitigate the problems of single-use plastics used for packaging food items: a review. J Food Sci Technol 2020;58:1-19. https://doi.org/10.1007/s13197-020-04885-6

4. Sojak L, Kubinec R, Jurdakova H, Hájeková E, Bajus M. GC-MS of polyethylene and polypropylene thermal cracking products. Petrol Coal 2006;48(1):1-14.

5. Lee DH, Kim Y, Fearing RS, Maboudian R. Effect of fiber geometry on macroscale friction of ordered low-density polyethylene nanofiber arrays. Langmuir 2011;27(17):11008-16. https://doi.org/10.1021/la201498u

6. Gupta KK, Devi D. Characteristics investigation on biofilm formation and biodegradation activities of Pseudomonas aeruginosa strain ISJ14 colonizing low density polyethylene (LDPE) surface. Heliyon 2020;6(7):e04398. https://doi.org/10.1016/j.heliyon.2020.e04398

7. Yoon MG, Jeon HJ, Kim MN. Biodegradation of polyethylene by a soil bacterium and AlkB cloned recombinant cell. J Biorem Biodegrad 2012;3(4):1-8.

8. Montazer Z, Najafi MBH, Levin DB. Challenges with verifying microbial degradation of polyethylene. Polymers 2020;12:123. https://doi.org/10.3390/polym12010123

9. Jeon HJ, Kim MN. Functional analysis of alkane hydroxylase system derived from Pseudomonas aeruginosa E7 for low molecular weight polyethylene biodegradation. Int Biodeterior Biodegrad 2015;103:141-6. https://doi.org/10.1016/j.ibiod.2015.04.024

10. Ghosh S, Qureshi A, Purohit HJ. Microbial degradation of plastics: biofilms and degradation pathways. Contaminants in agriculture and environment: health risks and remediation, Agriculture and Environmental Science Academy, Haridwar, India. vol. 1, pp 184-99, 2019. https://doi.org/10.26832/AESA-2019-CAE-0153-014

11. Koutny M, Lemaire J, Delort AM. Biodegradation of polyethylene films with prooxidant additives. Chemosphere 2006;64(8):1243-52. https://doi.org/10.1016/j.chemosphere.2005.12.060

12. Joo S, Cho IJ, Seo H, Son HF, Sagong HY, Shin TJ, et al. Structural insight into molecular mechanism of poly (ethylene terephthalate) degradation. Nat Commun 2018;9(1):1-12. https://doi.org/10.1038/s41467-018-02881-1

13. Deep A, Bhatt D, Shrivastav V, Bhardwaj SK, Malik P. Synthesis, characterization and applications of polyolefin based eco-friendly polymer composites. In: Sustainable polymer composites and nanocomposites, pp 65-103, 2019. https://doi.org/10.1007/978-3-030-05399-4_3

14. Pathak VM, Navneet. Review on the current status of polymer degradation: a microbial approach. Bioresour Bioprocess 2017; 4:1-31. https://doi.org/10.1186/s40643-017-0145-9

15. Sangale MK, Shahnawaz M, Ade AB. Gas chromatography-mass spectra analysis and deleterious potential of fungal based polythene-degradation products. Sci Rep 2019;9:1-6. https://doi.org/10.1038/s41598-018-37738-6

16. Sarkhel R, Sengupta S, Das P, Bhowal A. Comparative biodegradation study of polymer from plastic bottle waste using novel isolated bacteria and fungi from marine source. J Polym Res 2019;27(1):1-8. https://doi.org/10.1007/s10965-019-1973-4

17. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991;173:697-703. https://doi.org/10.1128/jb.173.2.697-703.1991

18. Santal AR, Singh NP, Saharan BS. Biodegradation and detoxification of melanoidin from distillery effluent using an aerobic bacterial strain SAG 5 of Alcaligenes faecalis. J Hazard Mater 2011;193:319-24. https://doi.org/10.1016/j.jhazmat.2011.07.068

19. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.

20. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547-9. https://doi.org/10.1093/molbev/msy096

21. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613-7. https://doi.org/10.1099/ijsem.0.001755

22. Abraham J, Ghosh E, Mukherjee P, Gajendiran A. Microbial degradation of low density polyethylene. Environ Progress Sustainable Energy 2017;36:147-54. https://doi.org/10.1002/ep.12467

23. Alaribe FO, Agamuthu P. Assessment of phytoremediation potentials of Lantana camara in Pb impacted soil with organic waste additives. Ecol Eng 2015;83:513-20. https://doi.org/10.1016/j.ecoleng.2015.07.001

24. Auta HS, Emenike CU, Jayanthi B, Fauziah SH. Growth kinetics and biodeterioration of polypropylene microplastics by Bacillus sp. and Rhodococcus sp. isolated from mangrove sediment. Mar Pollut Bull 2018;127:15-21. https://doi.org/10.1016/j.marpolbul.2017.11.036

25. Kay MJ, McCabe RW, Morton LHG. Chemical and physical changes occurring in polyester polyurethane during biodegradation. Int Biodeterior Biodegrad 1993;31(3):209-25. https://doi.org/10.1016/0964-8305(93)90006-N

26. Khandare SD, Chaudhary DR, Jha B. Marine bacterial biodegradation of low-density polyethylene (LDPE) plastic. Biodegradation 2021;32:1-17. https://doi.org/10.1007/s10532-021-09927-0

27. Al-Salem SM, Behbehani MH, Al-Hazza'a A, Arnold JC, Alston SM, Al-Rowaih AA, et al. Study of the degradation profile for virgin linear low-density polyethylene (LLDPE) and polyolefin (PO) plastic waste blends. J Mater Cycles Waste Manage 2019;21:1106-22. https://doi.org/10.1007/s10163-019-00868-8

28. Novotný ?, Malachová K, Adamus G, Kwiecie? M, Lotti N, Soccio M, et al. Deterioration of irradiation/high-temperature pretreated, linear low-density polyethylene (LLDPE) by Bacillus amyloliquefaciens. Int Biodeterior Biodegrad 2018;132:259-67. https://doi.org/10.1016/j.ibiod.2018.04.014

29. Esmaeili A, Pourbabaee AA, Alikhani HA, Shabani F, Esmaeili E. Biodegradation of low-density polyethylene (LDPE) by mixed culture of Lysinibacillus xylanilyticus and Aspergillus niger in soil. PLoS One 2013;8:e71720. https://doi.org/10.1371/journal.pone.0071720

30. Kyaw BM, Champakalakshmi R, Sakharkar MK, Lim CS, Sakharkar KR. Biodegradation of low density polythene (LDPE) by Pseudomonas species. Indian J Microbiol 2012;52:411-9. https://doi.org/10.1007/s12088-012-0250-6

31. Shahnawaz M, Sangale MK, Ade AB. Bacteria-based polythene degradation products: GC-MS analysis and toxicity testing. Environ Sci Pollut Res 2016;23:10733-41. https://doi.org/10.1007/s11356-016-6246-8

32. Singh S, Tripathi DK, Dubey NK, Chauhan DK. Effects of nano-materials on seed germination and seedling growth: striking the slight balance between the concepts and controversies. Mater Focus 2016;5:195-201. https://doi.org/10.1166/mat.2016.1329

33. Santal AR, Singh NP, Saharan BS. A novel application of Paracoccus pantotrophus for the decolorization of melanoidins from distillery effluent under static conditions. J Environ Manage 2016;169:78-83. https://doi.org/10.1016/j.jenvman.2015.12.016

34. Kebrom TH, Woldesenbet S, Bayabil HK, Garcia M, Gao M, Ampim P, et al. Evaluation of phytotoxicity of three organic amendments to collard greens using the seed germination bioassay. Environ Sci Pollut Res 2019;26(6):5454-62. https://doi.org/10.1007/s11356-018-3928-4

35. Sarker RK, Chakraborty P, Paul P, Chatterjee A, Tribedi P. Degradation of low-density poly ethylene (LDPE) by Enterobacter cloacae AKS7: a potential step towards sustainable environmental remediation. Arch Microbiol 2020;202:2117-25. https://doi.org/10.1007/s00203-020-01926-8

36. Bhatia M, Girdhar A, Tiwari A, Nayarisseri A. Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach. SpringerPlus 2014;3(1):1-10. https://doi.org/10.1186/2193-1801-3-497

37. Li Z, Wei R, Gao M, Ren Y, Yu B, Nie K, et al. Biodegradation of low-density polyethylene by Microbulbifer hydrolyticus {IRE}-31. J Environ Manage 2020;263:110402. https://doi.org/10.1016/j.jenvman.2020.110402

38. Amodu OS, Ojumu TV, Ntwampe SKO. Kinetic modelling of cell growth, substrate utilization, and biosurfactant production from solid agrowaste (Beta vulgaris) by Bacillus licheniformis STK 01. Can J Chem Eng 2016;94:2268-75. https://doi.org/10.1002/cjce.22631

39. Hadad D, Geresh S, Sivan A. Biodegradation of polyethylene by the thermophilic bacterium Brevibacillus borstelensis. J Appl Microbiol 2005;98:1093-100. https://doi.org/10.1111/j.1365-2672.2005.02553.x

40. Montazer Z, Habibi Najafi MB, Levin DB. Microbial degradation of low-density polyethylene and synthesis of polyhydroxyalkanoate polymers. Can J Microbiol 2019;65:224-34. https://doi.org/10.1139/cjm-2018-0335

41. Kumar D, Kumar L, Nagar S, Raina C, Parshad R, Gupta VK. Screening, isolation and production of lipase/esterase producing Bacillus sp. strain DVL2 and its potential evaluation in esterification and resolution reactions. Arch Appl Sci Res 2012;4(4):1763-70.

42. Montazer Z, Habibi-Najafi MB, Mohebbi M, Oromiehei A. Microbial degradation of UV-pretreated low-density polyethylene films by novel polyethylene-degrading bacteria isolated from plastic-dump soil. J Polym Environ 2018;26:3613-25. https://doi.org/10.1007/s10924-018-1245-0

43. Kunlere IO, Fagade OE, Nwadike BI. Biodegradation of low density polyethylene (LDPE) by certain indigenous bacteria and fungi. Int J Environ Stud 2019;76:428-40. https://doi.org/10.1080/00207233.2019.1579586

44. Mukherjee S, RoyChaudhuri U, Kundu PP. Biodegradation of polyethylene via complete solubilization by the action of Pseudomonas fluorescens, biosurfactant produced by Bacillus licheniformis and anionic surfactant. J Chem Technol Biotechnol 2017;93:1300-11. https://doi.org/10.1002/jctb.5489

45. Das MP, Kumar S. An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens. 3 Biotech 2015;5: 81-6. https://doi.org/10.1007/s13205-014-0205-1

46. Maroof L, Khan I, Yoo HS, Kim S, Park HT, Ahmad B, et al. Identification and characterization of low density polyethylene-degrading bacteria isolated from soils of waste disposal sites. Environ Eng Res 2021;26(3):109-19. https://doi.org/10.4491/eer.2020.167

47. Abdel-Kader MS, Ghorab MM, Alsaid MS, Alqasoumi SI. Design, synthesis, and anticancer evaluation of some novel thiourea, carbamimidothioic acid, oxazole, oxazolidine, and 2-amino-1- phenylpropyl-2-chloroacetate derived from L-norephedrine. Russ J Bioorg Chem 2016;42:434-40. https://doi.org/10.1134/S1068162016040026

48. Pramila R. Brevibacillus parabrevis, Acinetobacter baumannii and Pseudomonas citronellolis - potential candidates for biodegradation of low density polyethylene (LDPE). J Bacteriol Res 2012;4(1):9-14. https://doi.org/10.5897/JBR12.003

49. Shahnawaz M, Sangale MK, Ade AB. Analysis of the plastic degradation products. Bioremediation technology for plastic waste, Springer Nature, Switzerland, AG. pp 93-101, 2019. https://doi.org/10.1007/978-981-13-7492-0_9

50. Pathak VM, Kumar N. Implications of SiO 2 nanoparticles for in vitro biodegradation of low-density polyethylene with potential isolates of Bacillus Pseudomonas, and their synergistic effect on Vigna mungo growth. Energy Ecol Environ 2017;2:418-27. https://doi.org/10.1007/s40974-017-0068-5

51. Balestri E, Menicagli V, Ligorini V, Fulignati S, Raspolli Galletti AM, et al. Phytotoxicity assessment of conventional and biodegradable plastic bags using seed germination test. Ecol Indic 2019;102:569-80. https://doi.org/10.1016/j.ecolind.2019.03.005

52. Mittler R, Vanderauwera S, Gollery M, Van Breusegem F. Reactive oxygen gene network of plants. Trends Plant Sci 2004;9:490-8. https://doi.org/10.1016/j.tplants.2004.08.009

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