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Volume: 3, Issue: 2, March-April, 2015
Cytological Effects of Herbicide Butachlor 50 EC on Somatic cells of Triticum aestivum L.
N. K. Hemanth Kumar, Shobha Jagannath
The widespread use of the herbicides for weed control and crop productivity in modern agriculture exerts a threat on economically important crops by way of cytological damage to the cells of crop plant or side effects. In the present study the cytotoxic effects of herbicide butachlor were investigated in the somatic cells of wheat. The wheat grains were treated with different concentrations of herbicide (0.15-1.0ppm) at room temperature. The percentage mitotic index decreased significantly as the concentration of the herbicide increased when compared to control. The chromosomal abnormalities were found to be increased as the concentration of the herbicide increased when compared to control. The observed chromosomal irregularities were sticky chromatin, chromosomal bridge, nuclear lesion, scattered chromosome, fragmented metaphase, fragmented anaphase, multipolar chromosome and micronuclei. According to our findings we can say that butachlor can produce negative side effects on mitotic division in somatic cells of wheat.
Keywords: Butachlor, Triticum aestivum, Chromosomal abnormalities, Mitosis.