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Volume: 3, Issue: 1, Jan-Feb, 2015
DOI: 10.7324/JABB.2015.3103

Research Article

Optimization of oyster (Pleurotus ostreatus) mushroom cultivation using locally available substrates and materials in Debre Berhan, Ethiopia

Asmamaw Tesfaw1, Abebe Tadesse1, Gebre Kiros2

  Author Affiliations


Abstract

Techniques to grow oyster mushrooms from culture to harvest were evaluated using locally available materials. Oyster was successfully grown in Potato Dextrose Agar (PDA). Spawn for Pleurotus ostreatus was prepared from sorghum and wheat without showing significance preference on them. Wheat straw, barley straw, sinar straw, waste paper, and gabi wastes were used as substrates; waste paper and gabi wastes alone or in mixture with saw dust yielded more oyster than wheat straw, barley straw, sinar straw. Substrates were reused and the yield was slower when it is compared to original substrates and they were found contaminated during pasteurization. Effects of pore size, temperature, and relative humidity on growth of mushrooms were evaluated. Pin hole size, high temperature (25oC) and high relative humidity were optimal for oyster growth. This temperature is optimal for spawn running both in cultivation and spawn production.

Keywords:

Oyster mushroom, Pleorotus ostreatus, spawn, primordia, mushroom cultivation.



Citation: Asmamaw Tesfaw, Abebe Tadesse, Gebre Kiros. Optimization of oyster (Pleurotus ostreatus) mushroom cultivation using locally available substrates and materials in Debre Berhan, Ethiopia. J App Biol Biotech. 2015; 3 (01): 015-020. DOI: 10.7324/JABB.2015.3103


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Mandeel Q, Al-Laith A, Mohamed S. Cultivation of oyster mushrooms (Pleurotus spp.) on various lignocellulosic wastes. World Journal of Microbiology and Biotechnology. 2005;21(4):601-7.

2. Chang S-T. World Production of Cultivated Edible and Medicinal Mushrooms in 1997 with Emphasis on

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