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Volume: 3, Issue: 1, Jan-Feb, 2015
DOI: 10.7324/JABB.2015.3103

Research Article

Optimization of oyster (Pleurotus ostreatus) mushroom cultivation using locally available substrates and materials in Debre Berhan, Ethiopia

Asmamaw Tesfaw1, Abebe Tadesse1, Gebre Kiros2

  Author Affiliations


Techniques to grow oyster mushrooms from culture to harvest were evaluated using locally available materials. Oyster was successfully grown in Potato Dextrose Agar (PDA). Spawn for Pleurotus ostreatus was prepared from sorghum and wheat without showing significance preference on them. Wheat straw, barley straw, sinar straw, waste paper, and gabi wastes were used as substrates; waste paper and gabi wastes alone or in mixture with saw dust yielded more oyster than wheat straw, barley straw, sinar straw. Substrates were reused and the yield was slower when it is compared to original substrates and they were found contaminated during pasteurization. Effects of pore size, temperature, and relative humidity on growth of mushrooms were evaluated. Pin hole size, high temperature (25oC) and high relative humidity were optimal for oyster growth. This temperature is optimal for spawn running both in cultivation and spawn production.


Oyster mushroom, Pleorotus ostreatus, spawn, primordia, mushroom cultivation.

Citation: Asmamaw Tesfaw, Abebe Tadesse, Gebre Kiros. Optimization of oyster (Pleurotus ostreatus) mushroom cultivation using locally available substrates and materials in Debre Berhan, Ethiopia. J App Biol Biotech. 2015; 3 (01): 015-020. DOI: 10.7324/JABB.2015.3103

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.


1. Mandeel Q, Al-Laith A, Mohamed S. Cultivation of oyster mushrooms (Pleurotus spp.) on various lignocellulosic wastes. World Journal of Microbiology and Biotechnology. 2005;21(4):601-7.

2. Chang S-T. World Production of Cultivated Edible and Medicinal Mushrooms in 1997 with Emphasis on< i> Lentinus edodes(Berk.) Sing, in China. International Journal of Medicinal Mushrooms. 1999;1(4):291-300.

3. Obodai M, Cleland-Okine J, Vowotor K. Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. Journal of Industrial Microbiology and Biotechnology. 2003;30(3):146-9.

4. Sánchez C. Cultivation of Pleurotus ostreatus and other edible mushrooms. Applied microbiology and biotechnology. 2010;85(5):1321-37.

5. Zhang Y, Geng W, Shen Y, Wang Y, Dai Y-C. Edible Mushroom Cultivation for Food Security and Rural Development in China: BioInnovation, Technological Dissemination and Marketing. Sustainability. 2014;6(5):2961-73.

6. Garcha H, Khanna P, Soni G. Nutritional importance of mushrooms. In: Chang S, Buswell J, Chiu S, editors. Mushroom biology and mushroom products Chinese University. Hong Kong: Chinese University Press; 1993.

7. Tibuhwa DD. Wild mushroom-an underutilized healthy food resource and income generator: experience from Tanzania rural areas. Journal of ethnobiology and ethnomedicine. 2013;9(1):49.

8. World Bank. Ethiopia: Country economic memorandum Geneva World Bank, 2004.

9. Dawit A. Mushroom Cultivation: A practical approach. Addis Ababa: Birhannaselam printing enterprise; 1998.

10. Oei P, van Nieuwenhuijzen B. Small-scale mushroom cultivation: oyster, shiitake and wood ear mushrooms. Wageningen, The Netherlands: Digigrafi; 2005.

11. Oei P. Mushroom cultivation: appropriate technology for mushroom growers: Backhuys Publishers; 2003.

12. Sarker N, Hossain M, Sultana N, Mian I, IJ SK, Ruhul Amin S. Effect of Different pH Levels on the Growth and Yield of P. ostreatus (Jacquin ex Fr.) Kummer. Bangladesh Journal of Mushroom. 2007;1(1):57-67.

13. Stamets P. Growing gourmet and medicinal mushrooms: a companion guide to the mushroom cultivator. Hong Kong: Random House LLC; 2011.

14. Alam N, Lee J, Lee T. Mycelial growth conditions and phylogenetic relationships of Pleurotus ostreatus. World applied science journal 2010;9(8):928-37.

15. Nwokoye AI, Kuforiji O, Oni P. Studies on Mycelial Growth Requirements of Pleurotus Ostreatus (Fr.) Singer. International Journal of Basic and Applied Sciences. 2010;10(2):47-53.

16. Yildiz S, Yildiz ÜC, Gezer ED, Temiz A. Some lignocellulosic wastes used as raw material in cultivation of the< i> Pleurotus ostreatus culture mushroom. Process Biochemistry. 2002;38(3):301-6.

17. Baysal E, Peker H, Yalinkiliç MK, Temiz A. Cultivation of oyster mushroom on waste paper with some added supplementary materials. Bioresource Technology. 2003;89(1):95-7.

18. Pant D, Reddy UG, Adholeya A. Cultivation of oyster mushrooms on wheat straw and bagasse substrate amended with distillery effluent. World Journal of Microbiology and Biotechnology. 2006;22(3):267- 75.

19. Ruiz-Rodriguez A, Soler-Rivas C, Polonia I, Wichers HJ. Effect of olive mill waste (OMW) supplementation to Oyster mushrooms substrates on the cultivation parameters and fruiting bodies quality. International Biodeterioration & Biodegradation. 2010;64(7):638-45.

20. Marino RH, da Eira AF, Kuramae EE, Queiroz EC. Morphomolecular characterization of Pleurotus ostreatus (Jacq. Fr.) kummer strains in relation to luminosity and temperature of frutification. Scientia Agricola. 2003; 60(3): 531-535.

21. Zervakis G, Philippoussis A, Ioannidou S, Diamantopoulou P. Mycelium growth kinetics and optimal temperature conditions for the cultivation of edible mushroom species on lignocellulosic substrates. Folia microbiologica. 2001;46(3):231-4.

22. Sher H, Al-Yemeni M, Bahkali AH, Sher H. Effect of environmental factors on the yield of selected mushroom species growing in two different agro ecological zones of Pakistan. Saudi Journal of Biological Sciences. 2010;17(4):321-6.

23. Shah Z, Ashraf M, Ishtiaq M. Comparative study on cultivation and yield performance of oyster mushroom (Pleurotus ostreatus) on different substrates (wheat straw, leaves, sawdust). Pakistan Journal of Nutrition. 2004;3(3):158-60.

24. Prakash P, Aashish Bohra A, Neil J, Sivasubramnian. Effect of Humic Acid on Pleurotus Ostreatus Mushroom Cultivation and Analysis of Their Nutrient Contents. Research Journal of Agriculture and Biological Sciences. 2010;6(6):1067-70.

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