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Volume: 2, Issue: 6, Nov-Dec, 2014
DOI: 10.7324/JABB.2014.2605

Research Article

Clonal propagation of female plants of Garcinia indica Choiss: a tree species of high medicinal value

Swapna Deodhar, Kiran Pawar, Nitasha Singh, R.J. Thengane, S.R. Thengane


Fruits of Garcinia indica, a medicinally important tree species from Western Ghats of India is a rich source of medicinally important (-) Hydroxycitric acid that shows anticholesterol and antiobesity activities. Since this species is polygamodioecious, sex of the plant can be identified only after flowering that takes approximately 7-8 yrs. From commercial and horticultural point of view, plantation of female trees is much preferred. In this study, an efficient protocol for propagation of female plants was developed using in vitro techniques. For establishment of sterile cultures, fungal contamination and browning of explants were the major hurdles. Season of plant material collection and culture vessels used for in vitro sprouting were also observed significant factors in establishment of sterile cultures. The use of WPM basal fortified with 2% sucrose and 8.87 µM BAP resulted maximum sterile and healthy buds. TDZ in the range 0.22 – 4.54 µM along with BAP induced multiple shoots. Highest 63% rooting was obtained on WPM medium with 4.90 µM IBA whereas, maximum 60% plantlets survived in green house after their transfer to soil.

Keywords: Garcinia indica, Clonal propagation, medicinal plants.


1. Shara M, Ohia SE, Schmidt RE, Yasmin T, Zerdetta-Smith A, Kincaid A, Bagchi M, Chatterjee A, Bagachi D. and Stohs SJ. Physio-chemical properties of a novel (-) Hydroxycitric acid extract and its effect on body weight selected organ weight hepatic lipid peroxidation and DNA fragmentation hematology and clinical chemistry and histopathological changes over a period of 90 days. Molecular and Cellular Biochemistry. 2004; 260(1): 171-186.

2. Preuss HG, Bagchi D, Bagchi M, Rao CVS, Satyanarayana S and Dey DK. Efficiency of novel natural extract of (-) Hydroxycitric acid (HCA-SX) and a combination of HCA-SX niacin bound chromium and Gymnema sylvestre extract in weight management in human volunteers: A pilot study, Nutrition Research. 2004; 24: 55-58.

3. Jena S, Jayaprakasha GK, Singh P, and Sakariah KK. Chemistry and Biochemistry of (-) Hydroxy Citric Acid, Journal of Agriculture Food Chemistry. 2002; 50(1): 10-22.

4. Watson JA, Fang M, and Lowenstin JM. Tricarbollylate and hydrooxycitrate substrate and inhibitors of ATP: citrate oxaloacetate lyase, Archive of Biochemistry and Biophysics. 1969: 135: 209-217.

5. Reddy SY. and Prabhakar JV. Cocoa butter extenders from kokum (Garcinia indica) and phulwara (Madhuca butyraceae) butter, J,Ann, Oil Chem, Soc. 1994; 71: 217-219.

6. Sundaram BM, Gopalkrishnan C, Subramanian S. and Shankaranarayan D. Anti microbial activity of Garcinia mangostana. 1983; Planta Medica 4: 59-60,

7. Iwu MM, Igboko OA, Okunji CO. and Tempests MS, Anti diabetic and aldose reductase activities of bioflavanones of Garcinia kola. J, Pharm, Pharmacol. 1990; 42: 290-292.

8. Chen S, Wan M., Lok BN. Active constituents against HIV-1 protease from Garcinia mangostana, Planta Med. 1996; 62: 381-82.

9. George GL, Lakshmanan R, Mathew KM, and Rao TS. Review of research in kokum camboge and relates species, Journal of Spices and Aromatic Crops. 2002; 11: 1-12.

10. Malik SK, Chaudhary R, Abraham Z. Desiccation-freezing sensitivity and longevity in seeds of Garcinia indica G, combogia and G, xanthochymus, Seed Science Technology. 2005; 33 (2).

11. Haldankar PM, Salvi MJ, Joshi GD, and Patil JL. Factors influencing softwood grafting in kokam (Garcinia indica Choisy), Indian Cocoa Arecanut Spices Journal. 1993; 17: 15-18.

12. Nawala RN, Karmarkar MS. Softwood grafting technique for rapid multiplication of high yielding clones in kokum (Garcinia indica Choisy). Indian Cocoa Arecanut Spices Journal. 1997;21: 76-77.

13. Rajasekharan PE, and Ganeshan S. Conservation of medicinal plant biodiversity\-an Indian perspective. J Med Arom Plants. 2002; 24: 132.

14. Engelmann F. Importance of cryopreservation for the conservation of plant genetic resources, In Engelmann F Takagi H (Eds) Cryopreservation of Tropical Plant germplasm Current research progress and Application, JIRCAS Tsukuba Japan/IPGRI Rome Italy. 2000; 8-20.

15. Normah MN, Nor-Azza AB. and Alidun R. Factors affecting invitro shoot proliferation and exvitro estsblishment of mangosteen. Plant Cell Tissue Org, Cult. 1995; 43: 291-294,

16. Goh C, Lakshmanan P. and Loh C. High frequency direct shoot bud regeneration from excised leaves of mangosteen ( Garcinia mangostana L,). Plant Sci. 1994; 101: 173-180,

17. Te-Chato S. and Lim M. Plant regeneration of mangosteen via nodular callus formation. Plant Cell Tissue Org, Cult. 1999; 59: 89-93.

18. Thengane SR, Deodhar SR, Bhosle SV. and Rawal SK. Repetitive somatic embryogenesis and plant regeneration in Garcinia indica Choiss, In vitro cellular and developmental biology \— Plant. 2006; 42: 256-261.

19. Deodhar SR, Thengane RJ, and Thengane SR. De novo shoot regeneration from root cultures of Garcinia indica Choiss. Indian Journal of Experimental Biology. 2008; 46: 482-486.

20. Malik SK, Chaudhary R. and Kalia RJ. Rapid in vitro multiplication and conservation of Garcinia indica: A tropical medicinal tree species. Scientia Horticulture. 2005; 106: 539-553.

21. Kulkarni M. and Deodhar M. In vitro regeneration and hydroxycitric acid production in tissue culture of Garcinia indica Choiss, Indian J, Biotech. 2002; I: 301-304.

22. Deshpande RS, Chavan SS. and Dhonuske BL. In-vitro shoot regeneration in kokum (Garcinia indica Choiss). Annals of Plant Physiology. 1999; 13: 31-34.

23. Mathew KM, Rao YS, Kuruvilla KM, Lakshmanan R, George GL, Madhusoodanan KJ. and Potty SN. Multiple shoot regeneration in kokum and camboge. Journal of spices and aromatic crops. 2001; 10: 151-152.

24. Loyd C. and McCown B. Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot tip culture. Int, Plant Propagation Soc, Proc. 1980; 30: 421-427.

25. Snedecor GW. and Cochran WG. 1967; Statistical methods Mohan Primlani Oxford and IBH publishing Co, New Delhi 569-571.

26. Diaz-Sala C, Rey M, and Rodriguez R. In vitro establishment of cyclonal chain from nodal segments and apical buds of adult hazel (Corylus avellana L,). Plant Cell Tissue Organ culture. 1990; 23: 151-157.

27. Yu X. and Reed BM. A micropropagation system for hazelnut (Corylus avellana L,), HortScience. 1995; 30: 120-123.

28. Nas Mehmet Nuri. Inclusion of polyamines in the medium improves shoot elongation in hazelnut (Corylus avellana L,) Micropropagation, Turk J Agri For. 2004; 28: 189-194.

29. Das DK, Shiva Prakash N. and Bhalla-Sarin N. Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn,). Plant Cell Reports. 1999; 18: 691\—695.

30. Jaime A, Teixeira and Da Silva, Tissue culture of Chrysanthemum: A review. Propagation of ornamental plants. 2003; 3(2): 23-29.

31. Raj SK, Singh R, Pandey SK, and Singh BP. Agrobacterium mediated tomato transformation and regeneration of transgenic lines expressing tomato leaf curl virus coat protein gene for resistance against TLCV infection, Research communications. Current Science. 2005; 10(88): 1674-1679.

32. Sapountzakis G. and Tsaftaris AS In vitro regeneration and genetic transformation of parthenocarpic cucumber hybrid ISHS Acta Horticulturae. 2002; 579: II Balkan Symposium on Vegetables and Potatoes.

33. Niedz RP. Using isothiazolone biocides to control microbial and fungal contaminants in plant tissue cultures. Hort Technology. 1998; 8: 598\—601.

34. Babaoglu M. and Yorgancilar M. TDZ-specific plant regeneration in salad burnet. Plant Cell Tissue Organ Culture. 2000; 440: 31\—34.

35. Biroscikova M, Spisakova K, Liptak S, Pichler V. and Durkovie J. Micropropagation of mature wych elm (Ulmus glabra Huds,). Plant Cell Reports. 2004; 22: 640-644.

36. Hutchinson JF. Factors affecting shoot proliferation and root initiation in organ culture of apple 'Northern Spy'. Scientia Horticulture. 1984; 22: 347-358.

37. Litz RE. and Conover RA. Effect of sex type season and other factors on in vitro establishment and culture of Carica papaya L, explant. Journal of American society Horticulture Science. 1981; 106, 792-794.

38. Sutter EG. and Barker PB. In vitro propagation of mature Liquidambar atyraciflua. Plant Cell Tissue Organ Cultur. 1987; 5: 13-21,

39. Amin MN. and Jaiswal VS. Rapid clonal propagation of Guava through in vitro shoot proliferation on nodal explants of mature trees. Plant Cell Tissue Organ Culture. 1987; 9: 235-243,

40. McClelland Smith MAL. Vessel type Closure and explant orientation influence in vitro performance of five woody species. Horticulture Science. 1990; 25: 797-800.

41. Martin KP. Raid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour a rare rheophytic woody medicinal plant. 2003; Plant Cell reports. 21: 415-420.

42. Hiregoudar LV, Murthy HN, Bhat JG, Nayeem A, Hema BP, Hahn EJ. and Paek KY. Rapid clonal propagation of Vitex trifolia, Biologia plantarum. 2006; 50(2): 291-294.

43. Faisal M. and Anis M. Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia. Bilogia Plantarum. 2006; 50: 437-440.

44. Mehta UJ, Sahasrabudhe N. and Hazra S. Thidiazuron-induced morphogenesis in tamarind seedlings. In Vitro Cellular & Developmental Biology - Plant. 2005; 41(3): 240-243.

45. Mithila J, Hall JC, Victor JMR. and Saxena PK. Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl,). Plant Cell Reports. 2003; 21: 408\—414.

46. Neves LO, Tomaz L. and Fevereiro MPS. Micropropagation of Medicago truncatula Gaertn, cv, Jemalong and Medicago truncatula ssp, Narbonensis. Plant Cell Tissue and Organ Culture. 2001; 67 (1): 81-84.

47. Srivatanakul M, Park S, Sanders J, Salas M. and Smith R. Multiple bud regeneration of kenaf (Hibiscus cannabinus L,) from bud apex culture system, Plant Cell Reports. 2000; 19(12): 1165-1170.

48. Murthy BNS, Victor J, Singh RP, Fletcher RA. and Saxena PK. In vitro regeneration of chickpea (Cicer arietinum L,): stimulation of direct organogenesis and somatic embryogenesis by Thidiazuron. Plant Growth Regulators. 1996; 19: 233\—240.

49. Faisal M, Ahmad N. and Anis M. Shoot multiplication in Rauvolfia tetraphylla L, using thidiazuron. Plant Cell Tissue Organ Culture. 2005; 80: 187\—190.

50. Huetteman CA. and Preece JE. Thidiazuron: a potent cytokinin for woody plant cell tissue culture. Plant Cell Tissue Organ Culture. 1993; 33: 105\—121.

51. Chalupa V. Effect of Benzylaminopurine and Thidiazuron on in vitro shoot proliferation of Tilia cordata MILL, Sorbus aucuparia L, and Robinia pseudoacacia L. Biologia Plantarum. 1987; 29(6): 425-429.

52. Fukui H, Kano T, Takeuch S. and Nakamura M. In vitro growth of Japanese persimmon (Diospyros kaki Thunb,) seedling plants. Res, Bull, Fac, Agr, Gifu, Uni. 1987; 52: 25-30.

53. Bahrany Abdulaziz Swing. Effect of phytohormones on in vitro shoot multiplication and rooting of lime Citrus aurantifolia (Christm,). Scientia Horticulturae. 2002; 95(4): 285-295.

54. Sasikumar S, Raveendar S, Premkumar A, Ignacimuthu S. and Agastian P. Micropropagation of Baliospermum montanum (Willd) Muell, Arg, \— Athreatened medicinal plant. Indian J of Biotechnology. 2009; 8: 223-226.

55. George S, Geetha SP and Balachandran I. Micropropagation of Baliospermum montanum (Willd) Muell \— Arg \— A red listed medicinal plant. J of plant Sciences. 2008; 3(1): 111-115.

56. Haw AB. and Keng CL. Micropropagation of Spilanthes acmella L, a bio-insecticide plant through proliferation of multiple shoots. J Appl Hortic. 2003; 5(2): 65\—68.

57. Wala BB. and Jasrai YT. Micropropagation of an endangered medicinal plant: Curculigo orchioides Gaertn. Plant Tissue Culture. 2003; 13: 13\—19.

58. Joshi MS. and Thengane SR. In vitro propagation of Azadirachta indica A, Juss, (neem) by shoot proliferation. Indian Journal of Experimental Biology. 1996; 34: 480\—482.

How to cite this article:
Swapna Deodhar, Kiran Pawar, Nitasha Singh, Thengane RJ, Thengane SR. Clonal propagation of female plants of Garcinia indica Choiss: a tree species of high medicinal value. J App Biol Biotech. 2014; 2 (06): 018-025. DOI: 10.7324/JABB.2014.2605



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