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Volume: 2, Issue: 6, Nov-Dec, 2014

Nov-Dec, 2014

DOI: 10.7324/JABB.2014.2601

Research Article

Enzymes and qualitative phytochemical screening of endophytic fungi isolated from Lantana camara Linn. Leaves

Mbouobda Hermann Desire1 2, Fotso Bernard1 2, Muyang Rosaline Forsah1 3, Chiatoh Thaddeus Assang1, Omokolo Ndoumou Denis2

  Author Affiliations


Abstract

Plants harbor endophytes, capable of producing metabolites and enzymes with functions similar to host plant extracts. An inventory of Lantana camara’s endophytes could better explain the role played by extracts of this plant in its defense functions against pathogens. This study aimed at isolating and screening endophytic fungi from L. camara leaves for amylases, lipases and laccases production and for the production of some secondary metabolites using standard procedures. In-vitro culture techniques with Potato Dextrose Agar (PDA) as culture medium were used to isolate endophytes from L. camara leaf tissues. Isolates identification were done using macroscopic and microscopic characteristics. Three endopyhtic fungi were isolated and labeled LI, LII, and LIII; LI and LII had a slimy texture and produced no reproductive structures (spores, conidia, septate hyphae etc) hence identified as Mycelia sterilia. LIII was white, with an uneven surface growth, cottony dry aerial mycelium, visible hyphae with little branching and individual hyphae were septate, hence a Geotrichium sp. All isolates produced amylases and lipases while LIII solely produced laccase. The extract of LIII showed the presence of tannins and cardiac glycosides and absence of phenols and saponnins. The anti pathogenic roles of L. camara extract are thanks to the presence of endophytes in its tissues which produce enzymes that mediate plant defense functions as well as secondary metabolites with anti-pathogenic properties.

Keywords:

Endophytic fungi, extracellular enzymes, secondary metabolites, plant defense.



Citation: Mbouobda Hermann Desire, Fotso Bernard, Muyang Rosaline Forsah, Chiatoh Thaddeus Assang, Omokolo Ndoumou Denis. Enzymes and qualitative phytochemical screening of endophytic fungi isolated from Lantana camara Linn. Leaves. J App Biol Biotech. 2014; 2 (06): 001-006. DOI: 10.7324/JABB.2014.2601


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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