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Volume: 2, Issue: 5, Sep-Oct, 2014
Bioactivity assessment of endophytic fungi associated with Centella asiatica and Murraya koengii
Archana Nath, Jyoti Pathak and SR Joshi
The present study attempted to evaluate the antimicrobial and antioxidant activity of fungal endophytes associated with Centella asiatica and Murraya koengii, the ethnomedicinal plants used in traditional practices . Two endophytic fungi Aspergillus oryzae CeR1 and Colletotrichum gloeosporioides MKL1 were isolated from C. asiatica and M. koengii respectively which were characterized morphologically and by using rDNA- internal transcribed spacer. The total antioxidant power and free radical scavenging activity of the fungal extracts was estimated using the total phenolic content and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay along with the flavonoids and alkaloids content. The DPPH free radical assay showed that the ethanolic extract of endophytic fungi Aspergillus oryzae CeR1 had higher radical scavenging activity than Colletotrichum gloeosporioides MKL1. Antimicrobial activity of fungal extract tested against three bacterial strain namely, Staphylococcus aureus MTCC 96, Streptococcus pyogenes MTCC1925, Enteroccocus faecalis MTCC2729 and one fungal strain Candida albicans MTCC183 showed inhibition of growth of test organisms except Staphylococcus aureus MTCC96.
Keywords: Centella asiatica; Murraya koengii; Endophytic fungi; Ethanolic extract; Antioxidant; Antimicrobial.