Home >Current Issue

Volume: 7, Issue: 1, Jan-Feb, 2019
DOI: 10.7324/JABB.2019.70111

Research Article

Bioluminescence of Vibrio fischeri: A novel application for PSP quantification


Mohamed Blaghen1, 2, Abdel-hamid Abdallah Abakar1

  Author Affiliations


Abstract

Paralytic Shellfish Poison (PSP) are the most harmful neurotoxins create a serious public health problem. It is important to assess PSP in Shellfish destined for human consumption. However, recommended methods have some limitations for example in the case of Mouse Bioassay (MBA) showed a low sensitivity and reproducibility, and undesirability for ethical reasons; while physico-chemical techniques rest expensive and time-consuming. The main objective of this study, after discovered that PSP inhibited the luminescence of Vibrio fischeri, was the quantification of PSP by using Bioluminescence Inhibition Assay (BIA), and comparing the results obtained with those determined by MBA and LC-MS. Bivalve used were collected from Corniche Martil, Kabila and Oued Laou, along the Mediterranean coast of Morocco in Mars-2015. Results showed a weak correlation between LC-MS and MBA with r = 0.11, while, the correlation between LC-MS and BIA was very strong with r = 0.97, which suggests that, BIA could offer an interesting additional assessment of PSP risk. In addition, after seen its rapidity, ease, reliability, sensitivity, reproducibility and cost effectiveness, it would be eligible to use for monitoring in surveillance programs.

Keywords:

Bioluminescence, Vibrio fischeri, Acanthocardia Tuberculatum, bivalve, PSP, STX.



Citation: Blaghen M, Abakar AA. Bioluminescence of Vibrio fischeri: A novel application for PSP quantification. J App Biol Biotech. 2019;7(01):060- 064. DOI: 10.7324/JABB.2019.70111


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Wiese M, D'Agostino PM, Mihali TK, et al. Neurotoxic Alkaloids: STX and Its Analogs. Mar Drugs 2010; 8:2185-2211. https://doi.org/10.3390/md8072185

2. Vale C, Alfonso A, Vieytes MR, et al. In Vitro and in Vivo Evaluation of Paralytic Shellfish Poisoning Toxin Potency and the Influence of the pH of Extraction. Anal Chem 2008; 80:1770-1776. https://doi.org/10.1021/ac7022266

3. Wong C-K, Hung P, Ng EAL, et al. Operational application of a rapid antibody-based detection assay for first line screening of paralytic shellfish toxins in shellfish. Harmful Algae 2010; 9:636-646. https://doi.org/10.1016/j.hal.2010.05.004

4. Anderson DM, Alpermann TJ, Cembella AD, et al. The globally distributed genus Alexandrium: Multifaceted roles in marine ecosystems and impacts on human health. Harmful Algae 2012; 14:10-35. https://doi.org/10.1016/j.hal.2011.10.012

5. Lawrence JF, Niedzwiadek B, Menard C. Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection: collaborative study. J AOAC Int 2005; 88:1714-1732.

6. Lehane L. Paralytic shellfish poisoning: a potential public health problem. Med J Aust 2001; 175:29-31.

7. Lehane L, Australia. Dept. of Agriculture F, Animal FNO of, et al. Paralytic shellfish poisoning: a review. Canberra: Animal Health Science and Emergency Management Branch, Agriculture, Fisheries and Forestry.

8. Sommer H, Meyer KF. Paralytic Shell-Fish Poisoning. Arch Pathol 1937; 24:560-98.

9. Bricelj VM, Shumway SE. Paralytic Shellfish Toxins in Bivalve Molluscs: Occurrence, Transfer Kinetics, and Biotransformation. Rev Fish Sci 2010; 6:315-383. https://doi.org/10.1080/10641269891314294

10. Park DL, Guzman-Perez SE, Lopez-Garcia R. Aquatic biotoxins: design and implementation of seafood safety monitoring programs. Rev Environ Contam Toxicol 1999; 161:157-200. https://doi.org/10.1007/978-1-4757-6427-7_2

11. Fernández ML, Cembella AD. Mammalian bioassays. 1995. In: Hallegraeff GM, Anderson DM, Cembella AD, eds. Manual on Harmful Marine Microalgae. UNESCO, France 213-228.

12. Van Dolah FM, Ramsdell JS. Review and assessment of in vitro detection methods for algal toxins. J AOAC Int 2001; 84:1617-1625.

13. Li Z-Y, Chen J-H, Li X, et al. Rapid Screening, Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database. Chin J Anal Chem Chin VERSION 2013; 41:979. https://doi.org/10.1016/S1872-2040(13)60739-2

14. Botana LM, Vilario N, Alfonso A, et al. The problem of toxicity equivalent factors in developing alternative methods to animal bioassays for marine-toxin detection. TrAC Trends Anal Chem 2010; 29:1316-1325. https://doi.org/10.1016/j.trac.2010.09.004

15. Jaime E, Hummert C, Hess P, et al. Determination of paralytic shellfish poisoning toxins by highperformance ion-exchange chromatography. J Chroma - togr, 2001; 43-49. https://doi.org/10.1016/S0021-9673(01)01181-5

16. Fonfría ES, Vilari-o N, Campbell K, et al. Paralytic Shellfish Poisoning Detection by Surface Plasmon Resonance-Based Biosensors in Shellfish Matrixes. Anal Chem 2007; 79:6303-6311. https://doi.org/10.1021/ac070362q

17. Campbell K, Haughey SA, van den Top H, et al. Single laboratory validation of a surface plasmon resonance biosensor screening method for paralytic shellfish poisoning toxins. Anal Chem 2010; 82:2977- 2988. https://doi.org/10.1021/ac1000338

18. Haughey SA, Campbell K, Yakes BJ, et al. Comparison of biosensor platforms for surface plasmon resonance based detection of paralytic shellfish toxins. Talanta 2011; 85:519-526. https://doi.org/10.1016/j.talanta.2011.04.033

19. Oshima Y. 1995a. Post-column derivatization HPLC methods for paralytic shellfish poisons. In: Hallegraeff GM, Anderson DM, Cembella AD, eds. Manual on Harmful Marine Microalgae. UNESCO, France 81-94.

20. Oshima Y. Postcolumn derivatization liquid chromatographic method for paralytic shellfish toxins. J AOAC Int 1995b; 78:528-532.

21. Turrell E, Stobo L, Lacaze J-P, et al. Optimization of hydrophilic interaction liquid chromatography/mass spectrometry and development of solid-phase extraction for the determination of paralytic shellfish poisoning toxins. J AOAC Int 2008; 91:1372-1386.

22. Sayfritz SJ, Aasen JAB, Aune T. Determination of paralytic shellfish poisoning toxins in Norwegian shellfish by liquid chromatography with fluorescence and tandem mass spectrometry detection. Toxicon 2008; 52:330-340. https://doi.org/10.1016/j.toxicon.2008.06.001

23. Zhuo L, Yin Y, Fu W, et al. Determination of paralytic shellfish poisoning toxins by HILIC?MS/MS coupled with dispersive solid phase extraction. Food Chem 2013; 137:115-121. https://doi.org/10.1016/j.foodchem.2012.10.010

24. Boundy MJ, Selwood AI, Harwood DT, et al. Development of a sensitive and selective liquid chromatography? mass spectrometry method for high throughput analysis of paralytic shellfish toxins using graphitised carbon solid phase extraction. J Chromatogr A 2015; 1387:1-12. https://doi.org/10.1016/j.chroma.2015.01.086

25. Dell'Aversano C, Hess P, Quilliam MA. Hydrophilic interaction liquid chromatography--mass spectrometry for the analysis of paralytic shellfish poisoning (PSP) toxins. J Chromatogr A 2005; 1081:190-201. https://doi.org/10.1016/j.chroma.2005.05.056

26. Diener M, Erler K, Christian B, et al. Application of a new zwitterionic hydrophilic interaction chromatography column for determination of paralytic shellfish poisoning toxins. J Sep Sci 2007; 30:1821-1826. https://doi.org/10.1002/jssc.200700025

27. Gago-Martínez A, Moscoso SA, Le\ão Martins JM, et al. Effect of pH on the oxidation of paralytic shellfish poisoning toxins for analysis by liquid chromatography. J Chromatogr A 2001; 905:351-357. https://doi.org/10.1016/S0021-9673(00)00963-8

28. Mattarozzi M, Milioli M, Bianchi F, et al. Optimization of a rapid QuEChERS sample treatment method for HILIC-MS 2 analysis of paralytic shellfish poisoning (PSP) toxins in mussels. Food Control 2016; 60:138-145. https://doi.org/10.1016/j.foodcont.2015.07.027

29. Solino L, Sureda FX, Diogne J. Evaluation of okadaic acid, dinophysistoxin-1 and dinophysistoxin-2 toxicity on Neuro-2a, NG108-15 and MCF-7 cell lines. Toxicol In Vitro 2015; 29:59-62. https://doi.org/10.1016/j.tiv.2014.09.002

30. Rodriguez LP, Vilario N, Molg J, et al. Development of a Solid-Phase Receptor-Based Assay for the Detection of Cyclic Imines Using a Microsphere-Flow Cytometry System. Anal Chem 2013; 85:2340- 2347. https://doi.org/10.1021/ac3033432

31. Turner AD, Tarnovius S, Johnson S, et al. Testing and application of a refined rapid detection method for paralytic shellfish poisoning toxins in UK shellfish. Toxicon 2015; 100:32-41. https://doi.org/10.1016/j.toxicon.2015.04.004

32. Harrison K, Johnson S, Turner AD. Application of rapid test kits for the determination of paralytic shellfish poisoning (PSP) toxins in bivalve molluscs from Great Britain. Toxicon 2016; 119:352-361. https://doi.org/10.1016/j.toxicon.2016.06.019

33. Ben-Gigirey B, Rodriguez-Velasco ML, Villar-Gonzalez A, et al. Influence of the sample toxic profile on the suitability of a high performance liquid chromatography method for official paralytic shellfish toxins control. J Chromatogr A 2007; 1140:78-87. https://doi.org/10.1016/j.chroma.2006.11.048

34. Parvez S, Venkataraman C, Mukherji S. A review on advantages of implementing luminescence inhibition test (Vibrio fischeri) for acute toxicity prediction of chemicals. Environ Int 2006; 32:265-268. https://doi.org/10.1016/j.envint.2005.08.022

35. Boyd EM, Killham K, Wright J, et al. Toxicity assessment of xenobiotic contaminated groundwater using lux modified Pseudomonas fluorescens. Chemosphere 1997; 35:1967-1985. https://doi.org/10.1016/S0045-6535(97)00271-3

36. Reemtsma T, Putschew A, Jekel M. Industrial wastewater analysis: a toxicity-directed approach. Waste Manag 1999; 19:181-188. https://doi.org/10.1016/S0956-053X(99)00011-2

37. Devare M, Bahadir M. Biological monitoring of landfill leachate using plants and luminescent bacteria. Chemosphere 1994; 28:261-271. https://doi.org/10.1016/0045-6535(94)90123-6

38. Abdallah AA, Daher A, Belghmi K, et al. Detoxification Assessment of Inorganic Mercury by Bioluminescence of Vibrio fischeri. Res J Environ Toxicol 2017; 11:104-111. https://doi.org/10.3923/rjet.2017.104.111

39. AOAC, 2000. Official Method 959.08. Paralytic Shellfish Poison Biological Method. Final Action. AOAC Official Methods of Analysis, 17th edition. Gaithersburg, Maryland 59-61.

40. Staack RF, Varesio E, Hopfgartner G. The combination of liquid chromatography/tandem mass spectrometry and chip-based infusion for improved screening and characterization of drug metabolites. Rapid Commun Mass Spectrom; 19:618-626. https://doi.org/10.1002/rcm.1829

41. Turrell EA, Lacaze JP, Stobo L. Determination of paralytic shellfish poisoning (PSP) toxins in UK shellfish. Harmful Algae 2007; 6:438- 448. https://doi.org/10.1016/j.hal.2006.12.002

42. Oshiro M, Pham L, Csuti D, et al. Paralytic shellfish poisoning surveillance in California using the Jellett Rapid PSP test. Harmful Algae 2006; 5:69-73. https://doi.org/10.1016/j.hal.2005.05.004

Article Metrics

Similar Articles