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Volume: 6, Issue: 6, Nov-Dec, 2018
DOI: 10.7324/JABB.2018.60612

Research Article

Antibacterial and antifungal activities of leaf and stem of Marsilea minuta L. against selected microbial pathogens

Govindaraj Sabithira, Rajangam Udayakumar

  Author Affiliations


Abstract

Aim: The aim of the present study was to determine the antimicrobial activity of different solvent extracts of leaf and stem of Marsilea minuta (L.) against some selected pathogenic microorganisms. Materials and Methods: The antibacterial and antifungal studies were carried out by well diffusion method in the Research Laboratory, Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam - 612 001, Tamil Nadu, India, between October and December 2015. Wells of 6mm diameter were punched in the agar medium and filled with different volume of extracts (50mg/ml) like 50, 75 and 100μl contains 2.5, 3.75 and 5mg of extract, respectively. The antimicrobial activity of different solvents such as aqueous, methanol, ethanol, and diethyl ether extracts of the leaf and stem of M. minuta at different concentrations was analyzed against selected bacterial pathogens such as Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescens, and Streptococcus pyogenes and the fungal pathogens such as Aspergillus niger, Aspergillus flavus, Aspergillus terreus, Trichoderma viride, and Fusarium solani. Results: The leaf and stem extracts showed antibacterial and antifungal activities by the formation of zone of inhibition ranging from 07 ± 0.8 to 25 ± 0.9 mm. Among the tested concentrations, 5 mg of both leaf and stem extracts showed best antibacterial and antifungal activities than other concentrations such as 2.5 and 3.75 mg. The maximum level of zone of inhibition 25 ± 0.9 mm was observed in aqueous stem extract against K. pneumonia whereas the minimum level of zone of inhibition 8 ± 0.4 mm was observed in the methanol leaf extract against S. pyogenes. The antifungal activity of tested solvent extracts of leaf showed positive results against in all fungal strains used in the present study. The aqueous leaf extracts showed maximum level of zone of inhibition 25±0.9mm against T. viride. The methanol stem extracts showed the minimum level of zone of inhibition 07±0.8mm against A. terreus. Conclusion: The results of this study confirmed the antimicrobial activity of leaf and stem of M. minuta and it may be source for the discovery of novel antimicrobial compounds.

Keywords:

Marsilea minuta, Antimicrobial activity, Well diffusion method, Microorganisms, Leaf, Stem.



Citation: Sabithira G, Udayakumar R. Antibacterial and antifungal activities of leaf and stem of Marsilea minuta (L.) against selected microbial pathogens. J App Biol Biotech. 2018;6(06):71-78. DOI: 10.7324/JABB.2018.60612


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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