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Volume: 7, Issue: 1, Jan-Feb, 2019
DOI: 10.7324/JABB.2019.70109

Research Article

A study of endophytic fungi Neofusicoccum ribis from Gandaria (Bouea macrophylla Griffith) as enzyme inhibitor, antibacterial, and antioxidant


Trisanti Anindyawati1, Praptiwi2

  Author Affiliations


Abstract

This study aims to evaluate the potential of endophytic fungi isolated from Gandaria (Bouea macrophylla Griffith) as sources of enzyme inhibitor, antibacterial, and antioxidant and identify the chemical compounds of active extract. The endophytic fungi were identified as fungi closely related to the genus Neofusicoccum ribis based on sequence analysis of the 28S rDNA and internal transcribed spacer region. N. ribis was cultured on potato dextrose broth and extracted with hexane, ethyl acetate, and partition between methanol and ethyl acetate, respectively. Bioactivity screenings as enzyme inhibitor (acetylcholinesterase/acetylcholinesterase inhibitor (AChE) and β-glucosidase), antibacterial, and antioxidant were conducted by thin-layer chromatography-bioautography method. The chemical compounds were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the ethyl acetate extract of N. ribis had the activity as enzyme inhibitor for AChE and β-glucosidase. The minimum inhibitory concentration values of N. ribis extract against Staphylococcus aureus and Escherichia coli were in the range of 512–>512 µg/mL. Hexane extract had better antioxidant activity (IC50 = 479.66 µg/mL) than ethyl acetate extract (IC50 = 928.09–1102.60 µ g/mL). The GC-MS analysis showed that 12 identified chemical compounds were found in the ethyl acetate extract of N. ribis.

Keywords:

Endophytic fungi, Neofusicoccum ribis, Enzyme inhibitor, Antibacterial, Antioxidant.



Citation: Anindyawati T, Praptiwi P. A study of endophytic fungi Neofusicoccum ribis from Gandaria (Bouea macrophylla Griffith) as enzyme inhibitor, antibacterial, and antioxidant. J App Biol Biotech. 2019;7(01):48-53. DOI: 10.7324/JABB.2019.70109


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Petrini O, Sieber TN, Toti L, Viret O. Ecology, metabolite production, and substrate utilization in endophyticfungi. Nat. Toxin, 1992;1:185-196. https://doi.org/10.1002/nt.2620010306

2. Nair DN, Padmavathy S. Impact of endophyticmicroorganisms on plants, environment and humans. The Scientific World J, 2014; ID 250693.

3. Khaswar RN, Misra A, Gond SK, Stierle A, Stierle D. Anticancer compounds derived from fungal endophytes: their importance and future challenges. Nat Prod Rep,2011; 28: 1208-1228. https://doi.org/10.1039/c1np00008j

4. Zhou X, Zhu H, Liu L, Lin J, Tang K. A review: recent advances and future prospects of taxol-producing endophytic fungi. ApplMicrobiolBiotechnol, 2010; 86: 1707-1717. https://doi.org/10.1007/s00253-010-2546-y

5. Strobel GA. Endophytes as sources of bioactive products. Microbes Infect,2003a;5(6):535-544. https://doi.org/10.1016/S1286-4579(03)00073-X

6. Hussain H, KlicheSpory C, AlHarrasi A, AlRawahi A, Abbas G, Green IR, Schulz B, Krohn K, Shah A. Antimicrobial constituents from three endophytic fungi. Asian Pac J Trop Med, 2014; 7(1): S224-S227. https://doi.org/10.1016/S1995-7645(14)60236-4

7. Strobel GA, Daisy B. Bioprospecting for microbial endophytes and their natural products. MicrobiolMolBiolRev, 2003b; 67(4):491–502. https://doi.org/10.1128/MMBR.67.4.491-502.2003

8. Bhagobaty RK, Joshi SR. Metabolite profiling of endophytic fungal isolates of five ethno-pharmacologically important plants of Meghalaya, India. J Metabolomics SystBiol, 2011; 2(2):20–31.

9. Kaewpongumpai S,Poeaim S, Vanijajiva O. Sequence-related amplified polymorphism (SRAP) analysis for studying genetic characterization of Boueamacrophylla. Biodiversitas,2016; 17(1): 539-543. https://doi.org/10.13057/biodiv/d170221

10. Rajan NS, Bhat R, Karim, AA. Preliminary studies on the evaluation of nutritional composition of unripe and ripe 'Kundang'fruit (BoueamacrophyllaGriffith).Int'l Food Res J,2014;21(3): 985-990.

11. Rajan NS, Rajan, Bhat R. Antioxidant compounds and antioxidant activities in unripe and ripe kundang fruits (BoueamacrophyllaGriffith). Fruits, 2016; 71(1): 41-47. https://doi.org/10.1051/fruits/2015046

12. Yang Z, Zhang X, Duan D, Song Z, Yang M, Li S. Modified TLC bioautographic method for screening acetylcholinesterase inhibitors from plant extracts. J Sep Sci, 2009; 32: 3257-3259. https://doi.org/10.1002/jssc.200900266

13. Simoes-Pires CA, Hmicha B, Marston A, Hostettman K.A TLC bioautographic method for the detection of \a- and \ß-glucosidase inhibitors in plant extracts.Phytochem Anal, 2009;20(6): 511-515. doi: 10.1002/pca.1154. https://doi.org/10.1002/pca.1154

14. Perumal S, Pillai S, Cai LW, Mahmud R, Ramanathan S. Determination of minimum inhibitory concentration of Euphorbia hirta (L.) extracts by tetrazolium microplate Assay. J of Nat Products, 2012; 5: 68-76.

15. Lee SY, Mediani A, Ashikin NAH, Azliana ABS, Abas F. Antioxidant and \a-glucosidase inhibitory activities of the leaf and stem of selected traditional medicinal plants. Int'l Food Res. J, 2014; 21(1): 165-172.

16. Shahverdi AR, AbdolpourF, Monsef-Esfahani HR, Farsam H. A TLCbioautographic assay for the detection of nitrofurantoin resistance reversal compound.JChromatogr B AnalytTechnolBiomedLife Sci, 2007; 850: 528–530. https://doi.org/10.1016/j.jchromb.2006.11.011

17. Bag A, Chattopadhyay RR. 2015. Evaluation of Synergistic Antibacterial and Antioxidant Efficacy of Essential Oils of Spices and Herbs in Combination. PLOS ONE.https://doi.org/10.1371/journal.pone.0131321 [Accessed 16 October 2017]. https://doi.org/10.1371/journal.pone.0131321

18. Marston A, Kissling J, Hostettman KA. A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants.Phytochem Anal, 2002; 13(1):51- 54. https://doi.org/10.1002/pca.623

19. Dewanjee S,Gangopadhyay M, Bhattacharyaa N, Khanra R, Dua TK. Bioautography and its scope in the field of natural product chemistry. J of Pharm Anal, 2015; 5(2): 75-84. https://doi.org/10.1016/j.jpha.2014.06.002

20. Pandey S, Sree A, Dash SS and Sethi DP. A novel method for screening beta-glucosidase inhibitors. BMC Microbiology 2013; 13:55 http://www.biomedcentral.com/1471-2180/13/55 [Accessed 16 October 2017]. https://doi.org/10.1186/1471-2180-13-55

21. Mehta A, Zitzmann N, RuddPM, BlockTM, DwekRA. \a-Glucosidase inhibitors as potential broad-based anti-viral agents, FEBS Lett, 1998; 430: 17-22. https://doi.org/10.1016/S0014-5793(98)00525-0

22. Silva MTG, SimasSM, BatistaTGFM, Cardarelli P, Tomassini TCB. Studies on antimicrobial activity, in vitro, ofPhysalisangulata L. (Solanaceae) fraction and physalin B bringing out the importance of assay determination.Mem. Inst. Oswaldo Cruz, 2005; 100(7):779-782. https://doi.org/10.1590/S0074-02762005000700018

23. Pessini GL, Filho BPD, Nakamura CV, Cortez DAG. Antibacterial activity of extracts and neolignans from Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck. Mem Inst Oswaldo Cruz, 2003; 98(8): 1115-1120. https://doi.org/10.1590/S0074-02762003000800025

24. Kumar S, Pandey AK. Medicinal attributes of Solanumxanthocarpum fruit consumed by several tribal communities as food: an in vitro antioxidant, anticancer and antiHIV perspective. BMC Comp Alt Med,2014; 14:112–119. https://doi.org/10.1186/1472-6882-14-112

25. Akpuaka A, Ekwenchi MM, Dashak DA, Dildar A. Biological activities of characterized isolates of n-hexane extract of Azadirachtaindica A. Juss (Neem) leaves. New York SciJ, 2013;6(6):119-124.

26. Sharma D, Pramanik A, Agrawal PK. Evaluation of bioactive secondary metabolites from endophytic fungus Pestalotiopsisneglecta BAB-5510 isolated from leaves of CupressustorulosaD.Don. 3 Biotech, 2016;6: 210-214. DOI 10.1007/s13205-016-0518-3.

27. Belakhdar G, Benjouad A, Abdennebi EH.Determination of some bioactive chemical constituents from ThesiumhumileVahl. JMaterEnvironSci, 2015; 6(10): 2778-2783.

28. Nandhini, US, Sangareshwari S, Lata K. Gas chromatography-mass spectrometry analysis of bioactive constituents from the marine Streptomyces. Asian JPharm Clin Res,2015; 8(2): 244-246.

29. Muthusamy K, Gopal V, SivanandhamV. GC-MS analysis of bioactive components of karisalaikarpachooranam- a siddha poly herbal formulation. Int'l J of Phytopharm, 2015; 6(3): 131-136.

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