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Volume: 2, Issue: 2, March-April, 2014
Gene expression study of Saccharomyces cerivisae GPH1 gene in response to chemical modulators
Prasad M. P.
The aim of the present study was to evaluate the gene expression of GPH1 gene of Saccharomyces cerivisae after it was induced to different stress conditions. The S. cerivisae was procured from MTCC and grown in YPD medium with different modulators like NaCl, Glucose, Calcium chloride, Sodium chloride, Potassium Nitrate, Arabinose and Dipotassium hydrogen orthophosphate. The RNA was isolated and analyzed on formaldehyde gel electrophoresis and determined by Nanodrop spectrophotometer. The cDNA was synthesized and used for Real Time PCR. Quantification was based on a standard curve, prepared from samples of known template concentration. The concentration of unknown sample is then determined by Ct value. There is a varied response to glycogen metabolism in S. cerivisae to environmental stress conditions such as nutrient limitation and heat shock. This response is mediated, in part, by the regulation of the glycogen metabolic genes. The results indicated that gene expression was high in modulator 3 and lowest in 2. Environmental stress induces a number of glycogen metabolic genes, including GPH1, which encodes glycogen phosphorylase. This study confirmed induction patterns of gene encoding glycogen phosphorylase GPH1 after treatment with modulators. This study gives an insight to the transcriptional control of GPH1 after modulator treatment.
Keywords: Saccharomyces cerivisae, Gene Expression, Modulator, GPH1, RT-PCR.