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Volume: 7, Issue: 1, Jan-Feb, 2019
DOI: 10.7324/JABB.2019.70105

Research Article

Influence of silver nitrate and glutamine on in vitro organogenesis of Lota Bhot (Capsicum chinense Jacq.), an indigenous pungent pepper variety of Assam


Geetashree Bora1 2, Hemanta Kumar Gogoi1, Pratap Jyoti Handique2

  Author Affiliations


Abstract

Northeast India including Assam is one of the richest centers of pepper diversity. Chilli peppers are valued commercially owing to their pungency ingredient, capsaicin. Capsicum chinense variety Lota Bhot is an extremely pungent chilli pepper indigenous to Assam with tremendous potential for pharmaceutical applications. An attempt was made to standardize an efficient micropropagation protocol using silver nitrate (AgNO3 ) and glutamine as the key components alongside other plant hormones. In vitro propagation was carried out with seed and shoot tip explants. MS medium supplemented with different combinations of plant growth hormones (BAP, GA3 , NAA, and IBA), fortified with AgNO3 and glutamine was used for regeneration of Lota Bhot. Addition of AgNO3 and glutamine into the culture medium greatly improved proliferation in selected In vitro plantlets. A maximum number of multiple shots was recorded in plantlets derived from shoot tips. The process of flower induction and fruit formation In vitro were found to be more productive when treated with AgNO3 along with GA3 . IBA generated better results in the process of root initiation than NAA. The response of shoot tip explants towards In vitro processes was better than seed explants. Sterile soil enriched with vermicompost upgraded the chances of acclimatization of In vitro raised healthy plantlets.

Keywords:

Glutamine, In vitro propagation, Lota Bhot, and Silver nitrate.



Citation: Bora G, Gogoi HK, Handique PJ. Influence of silver nitrate and glutamine on in vitro organogenesis of Lota Bhot (Capsicum chinense Jacq.), an indigenous pungent pepper variety of Assam. J App Biol Biotech. 2019;7(01):021-028. DOI: 10.7324/JABB.2019.70105


Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

References

1. Sanatombi K, Sharma GJ. Micropropagation of Capsicum annum L. using axillary shoot explants. Sci. Hort 2007; 113: 96-99. https://doi.org/10.1016/j.scienta.2007.01.020

2. Bosland PW. Capsicums: Innovative uses of an ancient crop. In: J. Janick (ed.), Progress in new crops. Arlington;1996; 479-487.

3 Odland ML, Porter AM. A study of natural crossing in pepper (Capsicum frutescens L.). J. Am. Soc. Hort. Sci 1941; 38:585-588.

4 Franceschetti U. Natural cross pollination in pepper (Capsicum annuum L.). Proc. Eucarpia Meeting on Genetic and Breeding of Capsicum. Turin, Italy; 1971; 346-353.

5. Tanksley SD. High rates of cross-pollination in chile pepper. HortScience 1984; 19:580-582.

6. Bosland PW, Votava EJ. Peppers: vegetables and spice Capsicums. Crop Production Science in Horticulture. England; 2000; 12: 204.

7. De A K. Capsicum: The Genus Capsicum. Medicinal and aromatic plants. Industrial Profiles Taylor & Francis, London and New York; 2003; 33: 275.

8 Ramage CM, Leung DWM. Influence of BA and sucrose on the competence and determination of Pepper (Capsicum annum L var. Sweet Banana) hypocotyl cultures during shoot formation. Plant Cell Reports 1996; 15: 974-979. https://doi.org/10.1007/BF00231599

9. Franck-Duchenne M, Wang Y, Tahar SF, Beachy RN. In vitro stem elongation of sweet pepper in media containing 24-epi-brassinolide. Plant Cell Tiss. Org. Cult 1998; 53: 79- 84. https://doi.org/10.1023/A:1006071803855

10. Mihalka V, Fari M, Szasz A, Balazs E. Optimized protocols for efficient regeneration and gene transfer in pepper (Capsicum annuum L.). J. Plant Biotechnol 2000; 2: 143-149.

11. Mohiuddin AKM, Chowdhury MKU, Zaliha C, Abdullah, Napis S. Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration. Plant Cell, Tissue and Organ Cult 1997; 51: 75-78. https://doi.org/10.1023/A:1005814514409

12. Williams J, Pink DAC, Biddington NL. Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera. Plant Cell, Tissue and Organ Cult 1990; 21: 61-66. https://doi.org/10.1007/BF00034493

13. Wua LM, Weia YM, Zhenga YL. . Effects of silver nitrate on the tissue culture of immature wheat embryos. Russian J of Plant Physiol 2006; 53: 530–534. https://doi.org/10.1134/S1021443706040157

14 Sandra AT, Maira O. Effect of culture medium consistence and silver nitrate on micropropagation of two potato (Solanum tuberosum) cultivars. Rev. Colomb. Biotecnol 2013; 2: 55-62.

15. Sharma A, Kumar V, Giridhar P, Ravishankar GA. Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation. Electronic J of Biotech 2008; 11: 1-6. https://doi.org/10.2225/vol11-issue2-fulltext-8

16. Pawar B, Kale P, Bahurupe J, Jadhav A, Kale A, Pawar S. Proline and glutamine improve in vitro callus induction and subsequent shooting in rice. Science Direct Rice Science 2015; 22: 283-289. https://doi.org/10.1016/j.rsci.2015.11.001

17. Chowdhry CN, Tyagi AK, Maheshwari N, Maheshwari SC. 1993. Effect of L-proline and L-tryptophan on somatic embryogenesis and plantlet regeneration of rice (Oryza sativa L. cv. Pusa 169). Plant Cell Tiss Org 1993; 32: 357–36. https://doi.org/10.1007/BF00042300

18. Ge XJ, Chu ZH, Lin YJ, Wang SP. A tissue culture system for different germplasms of indica rice. Plant Cell Rep 2006; 25: 392–402. https://doi.org/10.1007/s00299-005-0100-7

19. Shahsavari E. Impact of tryptophan and glutamine on the tissue culture of upland rice. Plant Soil Environ 2011; 57: 7–10. https://doi.org/10.17221/116/2010-PSE

20 Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962;15:473-97. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

21 Song JY, Sivanesan I, An CG, Jeong BR. Adventitious shoot regeneration from leaf explants of miniature paprika (Capsicum annuum) 'Hivita Red' and 'Hivita Yellow'. African J of Biotechnol 2010; 9: 2768-2773.

22. Otroshy M, Moradi K, Nekouei MK. The effect of different cytokenins in propagation of Capsicum annuum by in vitro nodal cutting. Trakia J of Sci 2011; 9: 21-30.

23. Buzzy NS, Flick AC, Perez FB, Peniche MCM, Castillo PYZ, Ruiz AS, Colli AZ, Alonso OG, Ham MLM. Regeneration of Hebenero pepper (Capsicum chinense J.) via organogenesis. HortSci 2005; 40: 1829-1831.

24. Kehie M, Kumaria S, Tandon P. In vitro plantlet regeneration from nodal segments and shoot tips of Capsicum chinense Jacq. cv. Naga King Chili. Biotech 2012; 2:31–35. https://doi.org/10.1007/s13205-011-0025-5

25. Hyde CL, Phillips GC. Silver nitrate promotes shoot development and plant regeneration of chili pepper (Capsicum annuum L) via organogenesis. In Vitro Cell Dev. Biol. Plant 1996; 32: 72-80. https://doi.org/10.1007/BF02823134

26. Ashrafuzzaman M, Hossain MM, Ismail MR, Haque MS, Shahidullah SM, Zaman S. Regeneration potential of seedling explants of chilli (Capsicum annuum). African J of Biotechnol 2009; 8: 591-596.

27. Sheeja TE, Mandal AB. In vitro flowering and fruiting in tomato (Lycopersicon esculentum Mill.). AsPac J. Mol. Biol. Biotechnol 2003; 11: 37-42.

28. AnantasaranJ, Songklanakarin KK. Influence of medium formula and silver nitrate on in vitro plant regeneration of Zinnia cultivars. J. Sci. Technol 2008; 30:1-6.

29. Shahsavari E. Impact of tryptophan and glutamine on the tissue culture of upland rice. Plant Soil Environ 2011; 57: 7–10. https://doi.org/10.17221/116/2010-PSE

30. Beyer EM. Silver ion: A potent anti-ethylene agent in cucumber and tomato. HortSci 1976;11: 175-196.

31. Bais HP, Sudha GS, Ravishankar GA. Putrescine and AgNO3 influences shoot multiplication, in vitro flowering and endogenous titers of polyamines in Cichorium intybus L. cv. Lucknow Local. J of Plant Growth Regul 2000; 19: 238-248. ¬33. Kumari M, Patade VY, Ahmed Z. Establishment of high regeneration potential in sweet pepper (Capsicum annuum L) cultivars Yolo Wonder and California Wonder. World J of Sci and Technol 2012; 2:26-35.

32. Ebida AIA, Hu C. In vitro morphogenic responses and plant regeneration from pepper (Capsicum annum L. cv Early California Wonder) seedling explants. Plant Cell Rep 1993; 13: 107-110. https://doi.org/10.1007/BF00235301

34. Hegde V, Partap PS, Yadav RC. Plant regeneration from hypocotyl explants in Capsicum (Capsicum annuum L.). Int.J.Curr.Microbiol.App.Sci 2017; 6: 545-557. https://doi.org/10.20546/ijcmas.2017.607.066

35. Sanatombi K, Sharma GJ. In vitro propagation of Capsicum chinense Jacq. Biologia Plantarium 2008; 52:517–520. https://doi.org/10.1007/s10535-008-0100-x

36. Bodhipadma K, Leung DWM. In vitro fruiting and seed set of Capsicum annuum L. cv. Sweet banana. In Vitro Cellular and Developmental Biol –Plant 2003; 39: 536-539. https://doi.org/10.1079/IVP2003441

37. Siddique I, Anis M. Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L. Indian J of Biotechnol 2006; 5:303–308.

38. Azad H, Schoonbeek S, Mahmoudi H, Derudder B, Maeyer PD, Witlox F. Organic agriculture and sustainable food production system: Main potentials. Agric. Ecosyst. Environ 2011; 144: 92-94. https://doi.org/10.1016/j.agee.2011.08.001

39. Ponti TD, Rijk B, Martin K, Ittersum V. The crop yield gap between organic and conventional agriculture. Agricultural Systems 2012; 108: 1-9. https://doi.org/10.1016/j.agsy.2011.12.004

40 Bhat TA, Gupta M, Ganai MA, Ahanger RA, Bhat HA. Yield, soil health and nutrient utilization of field pea (Pisum sativum L.) as affected by phosphorus and biofertilizers under subtropical conditions of Jammu. International J of Modern Plant and Animal Sci 2013; 1: 1-8.

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